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    • 1. 发明授权
    • Lower-power laser and arc hybrid welding method and device
    • 低功率激光和电弧复合焊接方法及装置
    • US08969755B2
    • 2015-03-03
    • US13518364
    • 2010-12-21
    • Liming LiuGang SongZhaodong Zhang
    • Liming LiuGang SongZhaodong Zhang
    • B23K28/02B23K26/20B23K26/14B23K26/06B23K9/16
    • B23K28/02B23K9/16B23K26/0622B23K26/20B23K26/24B23K26/348
    • A low-power laser and arc hybrid welding method includes the steps of matching laser pulses with arc phases, and inducing compress arcs by the laser pulses. A laser peak pulse is triggered from half of the positive half-wave to half of the negative half-wave of alternating arc current. The sum of laser peak pulse width and laser basic pulse width is equal to the time width from a laser pulse triggered point to a negative half-wave end point of the alternating arc current. A welding device for carrying out the method is disclosed. An angle formed by the axis of a laser beam (1) and the vertical direction is in the range of −50°-50°. An angle formed by the axis of the laser beam (1) and the axis of an arc torch (2) is in the range of 20°-120°. Arc power density is adjusted minutely in the range of 103-105 by adjusting the alternating arc current, protrusion quantity of an electrode (3) and gas flow velocity of a nozzle. The method and the device can reduce the consumption and save the cost.
    • 低功率激光和电弧混合焊接方法包括以下步骤:将激光脉冲与电弧相匹配,并通过激光脉冲引发压缩弧。 激光峰值脉冲从正半波的一半触发到交变电弧电流的负半波的一半。 激光峰值脉冲宽度和激光基本脉冲宽度的总和等于从激光脉冲触发点到交变电弧电流的负半波终点的时间宽度。 公开了一种用于实施该方法的焊接装置。 由激光束(1)的轴线和垂直方向形成的角度在-50°-50°的范围内。 由激光束(1)的轴线和电弧割炬(2)的轴线形成的角度在20°-120°的范围内。 通过调节交流电弧电流,电极(3)的突出量和喷嘴的气体流速,可以在103-105的范围内微调电弧功率密度。 该方法和设备可以减少消耗并节省成本。
    • 2. 发明申请
    • Lower-Power Laser And ARC Hybrid Welding Method And Device
    • 低功率激光和ARC混合焊接方法和装置
    • US20130001205A1
    • 2013-01-03
    • US13518364
    • 2010-12-21
    • Liming LiuGang SongZhaodong Zhang
    • Liming LiuGang SongZhaodong Zhang
    • B23K26/00
    • B23K28/02B23K9/16B23K26/0622B23K26/20B23K26/24B23K26/348
    • A low-power laser and arc hybrid welding method includes the steps of matching laser pulses with arc phases, and inducing compress arcs by the laser pulses. A laser peak pulse is triggered from half of the positive half-wave to half of the negative half-wave of alternating arc current. The sum of laser peak pulse width and laser basic pulse width is equal to the time width from a laser pulse triggered point to a negative half-wave end point of the alternating arc current. A welding device for carrying out the method is disclosed. An angle formed by the axis of a laser beam (1) and the vertical direction is in the range of −50°-50°. An angle formed by the axis of the laser beam (1) and the axis of an arc torch (2) is in the range of 20°-120 °. Arc power density is adjusted minutely in the range of 103-105 by adjusting the alternating arc current, protrusion quantity of an electrode (3) and gas flow velocity of a nozzle. The method and the device can reduce the consumption and save the cost.
    • 低功率激光和电弧混合焊接方法包括以下步骤:将激光脉冲与电弧相匹配,并通过激光脉冲引发压缩弧。 激光峰值脉冲从正半波的一半触发到交变电弧电流的负半波的一半。 激光峰值脉冲宽度和激光基本脉冲宽度的总和等于从激光脉冲触发点到交变电弧电流的负半波终点的时间宽度。 公开了一种用于实施该方法的焊接装置。 由激光束(1)的轴线和垂直方向形成的角度在-50°-50°的范围内。 由激光束(1)的轴线和电弧割炬(2)的轴线形成的角度在20°-120°的范围内。 通过调节交流电弧电流,电极(3)的突出量和喷嘴的气体流速,可以在103-105的范围内微调电弧功率密度。 该方法和设备可以减少消耗并节省成本。
    • 3. 发明申请
    • Novel remombinant staphylokinase derivant and its preparation method and application thereof
    • 新型重组葡激酶衍生物及其制备方法及应用
    • US20050202000A1
    • 2005-09-15
    • US10182160
    • 2001-01-23
    • Houyan SongGang Song
    • Houyan SongGang Song
    • C07K14/31C12N15/54A61K38/48C07H21/04C12N9/64C12N15/09C12P21/06
    • C07K14/31
    • The present invention relates to the biotechnology field, more particularly, to novel recombinant staphylokinase (RGD/KGD-Sak) derivatives and the preparation the thereof. The derivatives, have a low polymerizing ability, low immunogenicity and a bifunctionality of thrombolytics and anticoagulant. Based on the line, structural analysis of the monomer and dimer of recombinant staphylokinases and their biochemical properties, we designed two novel bifunctional staphylokinase molecular structures. Mutant genes were constructed by PCR site-directed mutagenesis which were then recombined with a prokaryotic vector and used to transform E. coli. Engineered strains with a high expression level were selected by screening and propagated by fermentation, followed by disruption of the cells, centrifugation to collect inclusion bodies, renaturation, and purification of RGD/KGD-SAK through a two-step method. After lyophilized, the polymerizing ability and immunogenicity of the products decreased significantly. The derivatives can not only activate fibrinogen to lyse thrombus, but also significantly inhibit the platelet aggregation induced by ADP, suggesting that they have the bifunctionality of thrombolytics and anticoagulant.
    • 本发明涉及生物技术领域,更具体地涉及新型重组葡激酶(RGD / KGD-Sak)衍生物及其制备。 衍生物具有低聚合能力,低免疫原性和溶栓剂和抗凝血剂的双功能性。 基于线条,重组葡萄球菌激酶单体和二聚体的结构分析及其生物化学性质,设计了两种新型双功能葡萄球菌激酶分子结构。 突变基因通过PCR定点诱变构建,然后与原核载体重组,并用于转化大肠杆菌。 选择具有高表达水平的工程菌株,通过发酵筛选和繁殖,随后破碎细胞,通过两步法离心收集RGD / KGD-SAK的包涵体,复性和纯化。 冻干后,产物的聚合能力和免疫原性显着降低。 该衍生物不仅可以激活纤维蛋白原裂解血栓,还可以显着抑制ADP诱导的血小板聚集,这表明它们具有溶血栓剂和抗凝血剂的双功能性。