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1. 发明授权

US5667974A Method for detecting nucleic acid sequences using competitive amplification 失效
标题翻译：使用竞争性扩增检测核酸序列的方法
公开(公告)号：US5667974A
公开(公告)日：1997-09-16
申请号：US480220
申请日：1995-06-07
申请人： Larry Birkenmeyer , Isa K. Mushahwar
发明人： Larry Birkenmeyer , Isa K. Mushahwar
IPC分类号： C12Q1/68 , C12Q1/70 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6851 , C12Q1/6862 , C12Q1/706
摘要： The present invention provides a method for quantitatively detecting the amount of a target nucleic acid sequence which may be present in a test sample. A test sample which may contain a target nucleic acid sequence comprising target sequences X and Y is contacted with two primer sets, the first set being specific for target X and the second set being specific for target Y. The test sample also is contacted at the same time with an internal standard sequence IS, which is substantially derived from a combination of the first and second target sequences, and its corresponding oligonucleotide primers. Haptens are associated with the oligonucleotide primer sets in such a way that amplified target sequence products X and Y are detected by capture on a solid phase to which anti-hapten capture reagents are attached. A signal ratio of (X+Y)/S is determined to quantitate the amount of the target nucleic acid sequence contained in the sample.
摘要翻译：本发明提供了定量检测可能存在于测试样品中的靶核酸序列的量的方法。可以含有包含靶序列X和Y的靶核酸序列的测试样品与两个引物组接触，第一组对靶X是特异性的，第二组对靶标Y是特异性的。测试样品也在同时具有基本上衍生自第一和第二靶序列的组合的内标序列IS及其相应的寡核苷酸引物。半抗原与寡核苷酸引物组相关联，使得扩增的靶序列产物X和Y通过捕获在抗半抗原捕获试剂所连接的固相上而被检测。确定（X + Y）/ S的信号比来定量样品中包含的靶核酸序列的量。

2. 发明授权

US5955598A Primer compositions and kits for detecting hepatitis B virus 失效
标题翻译：用于检测乙型肝炎病毒的引物组合物和试剂盒
公开(公告)号：US5955598A
公开(公告)日：1999-09-21
申请号：US864404
申请日：1997-05-28
申请人： Larry Birkenmeyer , Isa K. Mushahwar
发明人： Larry Birkenmeyer , Isa K. Mushahwar
IPC分类号： C12Q1/68 , C12Q1/70 , C07H21/04
CPC分类号： C12Q1/6851 , C12Q1/6862 , C12Q1/706
摘要： The present invention provides a method for quantitatively detecting the amount of a target nucleic acid sequence which may be present in a test sample. A test sample which may contain a target nucleic acid sequence comprising target sequences X and Y is contacted with two primer sets, the first set being specific for target X and the second set being specific for target Y. The test sample also is contacted at the same time with an internal standard sequence IS, which is substantially derived from a combination of the first and second target sequences, and its corresponding oligonucleotide primers. Haptens are associated with the oligonucleotide primer sets in such a way that amplified target sequence products X and Y are detected by capture on a solid phase to which anti-hapten capture reagents are attached. A signal ratio of (X+Y)/S is determined to quantitate the amount of the target nucleic acid sequence contained in the sample.
摘要翻译：本发明提供了定量检测可能存在于测试样品中的靶核酸序列的量的方法。可以含有包含靶序列X和Y的靶核酸序列的测试样品与两个引物组接触，第一组对靶标X是特异性的，第二组对靶标Y是特异性的。测试样品也在同时具有基本上衍生自第一和第二靶序列的组合的内标序列IS及其相应的寡核苷酸引物。半抗原与寡核苷酸引物组相关联，使得扩增的靶序列产物X和Y通过捕获在抗半抗原捕获试剂所连接的固相上而被检测。确定（X + Y）/ S的信号比来定量样品中包含的靶核酸序列的量。

3. 发明授权

US5807670A Detection of hepatitis GB virus genotypes 失效
标题翻译：检测肝炎病毒基因型
公开(公告)号：US5807670A
公开(公告)日：1998-09-15
申请号：US580038
申请日：1995-12-21
申请人： Anthony Scott Muerhoff , John N. Simons , Thomas P. Leary , Larry Birkenmeyer , James C. Erker , Michelle Chalmers , George J. Dawson , Suresh M. Desai , Isa K. Mushahwar
发明人： Anthony Scott Muerhoff , John N. Simons , Thomas P. Leary , Larry Birkenmeyer , James C. Erker , Michelle Chalmers , George J. Dawson , Suresh M. Desai , Isa K. Mushahwar
IPC分类号： G01N33/566 , C07K14/18 , C12N15/09 , C12N15/113 , C12N15/86 , C12Q1/70 , G01N33/576
CPC分类号： C12N15/1131 , C07K14/005 , C12N15/86 , C12Q1/707 , C07K2319/00 , C12N2310/315 , C12N2310/3181 , C12N2310/33 , C12N2770/24222 , C12N2840/203 , C12Q1/6813
摘要： HGBV-C oligonucleotides from the 5' end of HGBV-C useful for the detection and genotyping of HGBV-C isolates. Also provided are assays which utilize these oligonucleotides.
摘要翻译：来自HGBV-C的5'端的HGBV-C寡核苷酸可用于HGBV-C分离物的检测和基因分型。还提供了利用这些寡核苷酸的测定法。

4. 发明授权

US5981172A Non-A, non-B, non-C, non-D, non-E Hepatitis reagents and methods for their use 失效
标题翻译：非A，非B，非C，非D，非E型肝炎试剂及其使用方法
公开(公告)号：US5981172A
公开(公告)日：1999-11-09
申请号：US417629
申请日：1995-04-06
申请人： John N. Simons , Tami J. Pilot-Matias , George J. Dawson , George G. Schlauder , Suresh M. Desai , Thomas P. Leary , Anthony Scott Muerhoff , Sheri L. Buijk , James Carl Erker , Isa K. Mushahwar
发明人： John N. Simons , Tami J. Pilot-Matias , George J. Dawson , George G. Schlauder , Suresh M. Desai , Thomas P. Leary , Anthony Scott Muerhoff , Sheri L. Buijk , James Carl Erker , Isa K. Mushahwar
IPC分类号： G01N33/53 , A61K38/00 , A61K39/00 , A61K39/29 , A61K39/395 , A61P1/16 , C07H21/04 , C07K14/02 , C07K14/08 , C07K14/10 , C07K14/18 , C07K16/10 , C12N5/10 , C12N15/09 , C12N15/40 , C12P21/02 , C12P21/08 , C12Q1/68 , C12Q1/70 , C12R1/19 , C12R1/92 , G01N33/531 , G01N33/566 , G01N33/576 , G01N33/577 , C07H21/02
CPC分类号： C07K14/005 , C12Q1/707 , G01N33/576 , A61K38/00 , A61K39/00 , C07K2319/00 , C12N2770/24222 , C12N2770/36022
摘要： Hepatitus GB Virus (HGBV) nucleic acid and amino acid sequences useful for a variety of diagnostic and theraeutic applications, kits for using the HGBV nucleic acid or amino acid sequences, HGBV immunogenic particles, and antibodies which specifically bind to HGBV. Also provided are methods for producing antibodies, polyclonal or monoclonal, from the HGBV nucleic acid or amino acid sequences.
摘要翻译：用于各种诊断和应用应用的肝癌GB病毒（HGBV）核酸和氨基酸序列，使用HGBV核酸或氨基酸序列的试剂盒，HGBV免疫原性颗粒和特异性结合HGBV的抗体。还提供了从HGBV核酸或氨基酸序列产生抗体，多克隆或单克隆抗体的方法。

5. 发明授权

US07556815B2 Hepatitis B virus surface antigen mutant and methods of detection thereof 失效
公开(公告)号：US07556815B2
公开(公告)日：2009-07-07
申请号：US11709364
申请日：2007-02-22
申请人： Paul F. Coleman , Isa K. Mushahwar
发明人： Paul F. Coleman , Isa K. Mushahwar
IPC分类号： A61K39/29 , C07H21/00 , C12N15/00 , C12N15/51 , G01N33/53
CPC分类号： C07K14/005 , C12N2730/10122 , G01N33/5764 , Y10S435/975 , Y10S530/826
摘要： The subject invention relates to a novel hepatitis B surface antigen mutant and methods of detecting this mutant, and/or antibodies thereto, in patient samples. In particular, the mutant contains a substitution of amino acid threonine for the amino acid alanine at position 123 in the amino acid sequence of the hepatitis B surface antigen (HBsAg) protein.

6. 发明授权

US07141242B2 Hepatitis B virus surface antigen mutant and methods of detection thereof 有权
公开(公告)号：US07141242B2
公开(公告)日：2006-11-28
申请号：US10847493
申请日：2004-05-17
申请人： Paul F. Coleman , Isa K. Mushahwar
发明人： Paul F. Coleman , Isa K. Mushahwar
IPC分类号： A61K39/29 , G01N33/53
CPC分类号： C07K14/005 , C12N2730/10122 , G01N33/5764 , Y10S435/975 , Y10S530/826
摘要： The subject invention relates to a novel hepatitis B surface antigen mutant and methods of detecting this mutant, and/or antibodies thereto, in patient samples. In particular, the mutant contains a substitution of amino acid threonine for the amino acid alanine at position 123 in the amino acid sequence of the hepatitis B surface antigen (HBsAg) protein.

7. 发明授权

US06455255B1 Method of performing subtractive hybridization using RDA 失效
标题翻译：使用RDA进行减法杂交的方法
公开(公告)号：US06455255B1
公开(公告)日：2002-09-24
申请号：US09631349
申请日：2000-08-02
申请人： Larry G. Birkenmeyer , Thomas P. Leary , A. Scott Muerhoff , Suresh M. Desai , Isa K. Mushahwar
发明人： Larry G. Birkenmeyer , Thomas P. Leary , A. Scott Muerhoff , Suresh M. Desai , Isa K. Mushahwar
IPC分类号： C12Q168
CPC分类号： C12Q1/6809 , C12Q2525/131 , C12Q2521/501
摘要： The subject invention relates to improvements in nucleic acid isolation, and more particularly, relates to modifications to the subtractive hybridization method and to reagents such as oligonucleotides that are useful when performing the method.
摘要翻译：本发明涉及核酸分离的改进，更具体地涉及减法杂交方法的修饰以及在进行该方法时有用的诸如寡核苷酸的试剂。

8. 发明授权

US5843450A Hepatitis GB Virus synthetic peptides and uses thereof 失效
标题翻译：乙型肝炎病毒合成肽及其用途
公开(公告)号：US5843450A
公开(公告)日：1998-12-01
申请号：US473475
申请日：1995-06-07
申请人： George J. Dawson , Tami J. Pilot-Matias , Dominique P. Bridon , Pamella A. Schroeder-Poliak , Mark F. Knigge , Keeve D. Jaffe , Isa K. Mushahwar
发明人： George J. Dawson , Tami J. Pilot-Matias , Dominique P. Bridon , Pamella A. Schroeder-Poliak , Mark F. Knigge , Keeve D. Jaffe , Isa K. Mushahwar
IPC分类号： G01N33/53 , A61K38/00 , A61K39/00 , A61K39/395 , C07K14/005 , C07K14/02 , C07K14/10 , C07K14/18 , C07K16/00 , C07K16/08 , C07K16/10 , C12N15/09 , C12P21/02 , C12P21/08 , C12Q1/70 , G01J3/453 , G01N33/576 , A61K39/12
CPC分类号： C07K14/005 , C07K16/109 , C12Q1/707 , G01N33/576 , A61K38/00 , A61K39/00 , C07K2319/00 , C12N2770/24222 , C12N2770/36022 , Y10S436/82 , Y10S977/918
摘要： Hepatitis GB Virus (HGBV) synthetic peptides useful for a variety of diagnostic and therapeutic applications, kits for using the HGBV nucleic acid or amino acid sequences and antibodies which specifically bind to HGBV. Also provided are methods for producing antibodies, polyclonal or monoclonal, from the HGBV peptides.
摘要翻译：可用于各种诊断和治疗应用的乙型肝炎病毒（HGBV）合成肽，使用HGBV核酸或氨基酸序列的试剂盒和特异性结合HGBV的抗体。还提供了用于从HGBV肽产生抗体，多克隆或单克隆抗体的方法。

9. 发明授权

US07202354B2 Hepatitis B virus surface antigen mutant and methods of detection thereof 失效
标题翻译：乙型肝炎病毒表面抗原突变体及其检测方法
公开(公告)号：US07202354B2
公开(公告)日：2007-04-10
申请号：US09821877
申请日：2001-03-30
申请人： Paul F. Coleman , Isa K. Mushahwar
发明人： Paul F. Coleman , Isa K. Mushahwar
IPC分类号： C07H21/00 , C12N15/00 , C12N15/51 , A61K39/29 , G01N33/53
CPC分类号： C07K14/005 , C12N2730/10122 , G01N33/5764 , Y10S435/975 , Y10S530/826
摘要： The subject invention relates to a novel hepatitis B surface antigen mutant and methods of detecting this mutant, and/or antibodies thereto, in patient samples. In particular, the mutant contains a substitution of amino acid threonine for the amino acid alanine at position 123 in the amino acid sequence of the hepatitis B surface antigen (HBsAg) protein.
摘要翻译：本发明涉及一种新型乙型肝炎表面抗原突变体和在该患者样品中检测该突变体和/或其抗体的方法。特别地，突变体含有氨基酸苏氨酸取代乙型肝炎表面抗原（HBsAg）蛋白质的氨基酸序列中123位的氨基酸丙氨酸。

10. 发明授权

US06558898B1 Non-A, non-B, non-C, non-D, non-E hepatitis reagents and methods for their use 失效
公开(公告)号：US06558898B1
公开(公告)日：2003-05-06
申请号：US08488446
申请日：1995-06-07
申请人： John N. Simons , Tami J. Pilot-Matias , George J. Dawson , George G. Schlauder , Suresh M. Desai , Thomas P. Leary , Anthony Scott Muerhoff , James Carl Erker , Sheri L. Buijk , Isa K. Mushahwar
发明人： John N. Simons , Tami J. Pilot-Matias , George J. Dawson , George G. Schlauder , Suresh M. Desai , Thomas P. Leary , Anthony Scott Muerhoff , James Carl Erker , Sheri L. Buijk , Isa K. Mushahwar
IPC分类号： C12Q170
CPC分类号： C07K14/005 , A61K39/00 , C07K16/109 , C07K2319/00 , C12N2770/24222 , C12Q1/707 , G01N33/576 , Y10S435/81 , Y10S530/826 , Y10S977/906 , Y10S977/915
摘要： Hepatitis GB Virus (HGBV) nucleic acid and amino acid sequences useful for a variety of diagnostic and therapeutic applications, kits for using the HGBV nucleic acid or amino acid sequences, HGBV immunogenic particles, and antibodies which specifically bind to HGBV. Also provided are methods for producing antibodies, polyclonal or monoclonal, from the HGBV nucleic acid or amino acid sequences.

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