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1. 发明申请

US20180292320A1 METHODS AND SYSTEMS FOR BIOLOGICAL INSTRUMENT CALIBRATION 审中-公开
公开(公告)号：US20180292320A1
公开(公告)日：2018-10-11
申请号：US16010359
申请日：2018-06-15
申请人： Life Technologies Corporation
发明人： Yong Chu , Jeffrey Marks , Jacob Freudenthal , Thomas Wessel , David Woo
IPC分类号： G01N21/64 , C12Q1/686 , G01N21/27 , C12Q1/6851
摘要： In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging fluorescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.

2. 发明授权

US10385383B2 Systems and methods for biological analysis 有权
公开(公告)号：US10385383B2
公开(公告)日：2019-08-20
申请号：US15048324
申请日：2016-02-19
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Ronald Danehy , Gary Lim , Jacob Freudenthal
IPC分类号： C12Q1/6806 , C12M1/32 , C12M1/00 , B01L3/00
摘要： A case for containing a plurality of biological samples contains a base, a substrate and a cover. The substrate is attached to the base and comprises a plurality of reaction regions configured to receive one or more biological samples. The cover comprises an inner surface and is configured to be attached to the base such that the base and cover together provide a cavity containing the substrate. The inner surface includes a central area and a recessed area disposed about the central area. The central area is configured to cover the reaction regions, while the recessed area is offset from the central area in a direction normal to the inner surface.

3. 发明申请

US20160230209A1 System and Methods for Calibrating Binding Dyes 审中-公开
标题翻译：校准结合染料的系统和方法
公开(公告)号：US20160230209A1
公开(公告)日：2016-08-11
申请号：US15017034
申请日：2016-02-05
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Yong Chu , Jacob Freudenthal , Jeffrey Marks
IPC分类号： C12Q1/68 , G01N21/64
CPC分类号： C12Q1/686 , G01N21/274 , G01N21/6428 , G01N21/6452 , G01N21/6456 , G01N2021/6421 , G01N2021/6441 , G01N2201/127
摘要： Some embodiments describe a computer-implemented method for calibrating a fluorescent dye. The method can comprise imaging a sample holder, loaded into an instrument, at more than one channel. The sample holder can comprise a plurality of reaction sites and more than one dye type, with each dye occupying more than one reaction site. The method can further comprise identifying a peak channel for each dye on the sample holder, normalizing each channel to the peak channel for each dye, and producing a dye matrix that can comprise a set of dye reference values.
摘要翻译：一些实施例描述了用于校准荧光染料的计算机实现的方法。该方法可以包括在多个通道上成像加载到仪器中的样品架。样品架可以包括多个反应位点和多于一种染料类型，每种染料占据多于一个反应位点。该方法还可以包括鉴定样品架上每种染料的峰通道，将每个通道标准化为每种染料的峰通道，以及产生可包含一组染料参考值的染料基质。

4. 发明授权

US10501788B2 System and methods for calibrating binding dyes 有权
公开(公告)号：US10501788B2
公开(公告)日：2019-12-10
申请号：US15017034
申请日：2016-02-05
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Yong Chu , Jacob Freudenthal , Jeffrey Marks
IPC分类号： G01N33/48 , C12Q1/686 , G01N21/27 , G01N21/64 , G06G7/58
CPC分类号： C12Q1/686 , G01N21/274 , G01N21/6428 , G01N21/6452 , G01N21/6456 , G01N2021/6421 , G01N2021/6441 , G01N2201/127
摘要： Some embodiments describe a computer-implemented method for calibrating a fluorescent dye. The method can comprise imaging a sample holder, loaded into an instrument, at more than one channel. The sample holder can comprise a plurality of reaction sites and more than one dye type, with each dye occupying more than one reaction site. The method can further comprise identifying a peak channel for each dye on the sample holder, normalizing each channel to the peak channel for each dye, and producing a dye matrix that can comprise a set of dye reference values.

5. 发明授权

US10147182B2 Methods and systems for streamlining optical calibration 有权
公开(公告)号：US10147182B2
公开(公告)日：2018-12-04
申请号：US15350444
申请日：2016-11-14
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Jacob Freudenthal , Jeffrey Marks , Francis Cheng , Thomas Wessel
IPC分类号： G06K9/00 , G06T7/00 , G06T5/00 , G06T5/50
摘要： A method for calibrating a biological instrument is provided. The method comprises the steps of acquiring an image of at least one biological sample array, determining a first region of interest within the image, wherein the first region of interest comprises a first plurality of locations on the at least one biological array; and identifying within the first region of interest, a plurality of image elements associated with each of the first plurality of locations on the at least one biological array.

6. 发明申请

US20160231245A1 METHODS AND SYSTEMS FOR INSTRUMENT VALIDATION 审中-公开
标题翻译：仪器验证的方法和系统
公开(公告)号：US20160231245A1
公开(公告)日：2016-08-11
申请号：US15016485
申请日：2016-02-05
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Thomas Wessel , Yong Chu , Jacob Freudenthal , David Woo
IPC分类号： G01N21/64
CPC分类号： G01N21/6428 , G01N21/274 , G01N21/6452 , G01N21/6456 , G01N2021/6439 , G16B40/00
摘要： In one exemplary embodiment, a method for validating an instrument is provided. The method includes receiving amplification data from a validation plate to generate a plurality of amplification curves. The validation plate includes a sample of a first quantity and a second quantity, and each amplification curve includes an exponential region. The method further includes determining a set of fluorescence thresholds based on the exponential regions of the plurality of amplification curves and determining, for each fluorescence threshold of the set, a first set of cycle threshold (Ct) values of amplification curves generated from the samples of the first quantity and a second set of Ct values of amplification curves generated from the samples of the second quantity. The method includes calculating if the first and second quantities are sufficiently distinguishable based on Ct values at each of the plurality of fluorescence thresholds.
摘要翻译：在一个示例性实施例中，提供了用于验证仪器的方法。该方法包括从验证板接收放大数据以产生多个扩增曲线。验证板包括第一数量和第二数量的样本，并且每个扩增曲线包括指数区域。该方法还包括基于多个扩增曲线的指数区域来确定一组荧光阈值，并且针对该组的每个荧光阈值确定从样品中产生的扩增曲线的第一组循环阈值（Ct）值从第二数量的样本产生的第一数量和第二组扩增曲线的Ct值。该方法包括基于多个荧光阈值中的每个荧光阈值处的Ct值来计算第一和第二量是否足够可区分。

7. 发明授权

US10012590B2 Methods and systems for biological instrument calibration 有权
公开(公告)号：US10012590B2
公开(公告)日：2018-07-03
申请号：US15017249
申请日：2016-02-05
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Yong Chu , Jeffrey Marks , Jacob Freudenthal , Thomas Wessel , David Woo
IPC分类号： H01J40/14 , G01N21/64 , C12Q1/686 , G01N21/27 , C12Q1/6851
CPC分类号： G01N21/6428 , C12Q1/6851 , C12Q1/686 , G01N21/274 , G01N21/278 , G01N21/6452 , G01N21/6456 , G01N2021/6439 , G01N2021/6471 , G01N2201/127 , G01N2201/13 , G01N2333/922
摘要： In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.

8. 发明申请

US20160231246A1 METHODS AND SYSTEMS FOR BIOLOGICAL INSTRUMENT CALIBRATION 有权
标题翻译：生物仪器校准的方法和系统
公开(公告)号：US20160231246A1
公开(公告)日：2016-08-11
申请号：US15017249
申请日：2016-02-05
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Yong Chu , Jeffrey Marks , Jacob Freudenthal , Thomas Wessel , David Woo
IPC分类号： G01N21/64 , C12Q1/68
CPC分类号： G01N21/6428 , C12Q1/6851 , C12Q1/686 , G01N21/274 , G01N21/278 , G01N21/6452 , G01N21/6456 , G01N2021/6439 , G01N2021/6471 , G01N2201/127 , G01N2201/13 , G01N2333/922
摘要： In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.
摘要翻译：在一个示例性实施例中，提供了一种用于校准仪器的方法。该仪器包括能够成像来自多个反应位点的荧光发射的光学系统。该方法包括执行感兴趣区域（ROI）校准以确定图像中的反应位置位置。该方法还包括进行纯染料校准，以通过将荧光染料的原始光谱与荧光染料的纯光谱校准数据进行比较来确定每个反应位点中使用的荧光染料的贡献。该方法还包括执行仪器归一化校准以确定滤波器归一化因子。该方法包括执行RNase P验证以验证仪器能够区分两种不同数量的样品。

9. 发明申请

US20140242596A1 Systems and Methods for Biological Analysis 有权
标题翻译：生物分析系统与方法
公开(公告)号：US20140242596A1
公开(公告)日：2014-08-28
申请号：US14348881
申请日：2012-09-28
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Ronald Danehy , Gary Lim , Jacob Freudenthal
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , B01L3/50851 , B01L3/50855 , B01L3/50857 , B01L2200/026 , B01L2200/0642 , B01L2200/0684 , B01L2200/142 , B01L2300/0819 , B01L2300/0829 , B01L2300/0858 , B01L2300/0893 , B01L2300/161 , C12M23/12 , C12M23/38
摘要： A case for containing a plurality of biological samples contains a base, a substrate and a cover. The substrate is attached to the base and comprises a plurality of reaction regions configured to receive one or more biological samples. The cover comprises an inner surface and is configured to be attached to the base such that the base and cover together provide a cavity containing the substrate. The inner surface includes a central area and a recessed area disposed about the central area. The central area is configured to cover the reaction regions, while the recessed area is offset from the central area in a direction normal to the inner surface.
摘要翻译：用于容纳多个生物样品的壳体包含基底，基底和盖。底物附着到基底上，并且包括被配置成接收一个或多个生物样品的多个反应区域。盖子包括内表面，并且被构造成附接到基座，使得基座和盖子一起提供容纳基底的空腔。内表面包括中心区域和围绕中心区域设置的凹陷区域。中心区域构造成覆盖反应区域，而凹陷区域在垂直于内表面的方向上偏离中心区域。

10. 发明授权

US10648912B2 Methods and systems for instrument validation 有权
公开(公告)号：US10648912B2
公开(公告)日：2020-05-12
申请号：US15016485
申请日：2016-02-05
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Thomas Wessel , Yong Chu , Jacob Freudenthal , David Woo
IPC分类号： G01N21/64 , G01N21/27 , G16B40/00
摘要： In one exemplary embodiment, a method for validating an instrument is provided. The method includes receiving amplification data from a validation plate to generate a plurality of amplification curves. The validation plate includes a sample of a first quantity and a second quantity, and each amplification curve includes an exponential region. The method further includes determining a set of fluorescence thresholds based on the exponential regions of the plurality of amplification curves and determining, for each fluorescence threshold of the set, a first set of cycle threshold (Ct) values of amplification curves generated from the samples of the first quantity and a second set of Ct values of amplification curves generated from the samples of the second quantity. The method includes calculating if the first and second quantities are sufficiently distinguishable based on Ct values at each of the plurality of fluorescence thresholds.

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