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1. 发明授权

US06905825B2 Method for isolating and purifying nucleic acids 有权
标题翻译：分离和纯化核酸的方法
公开(公告)号：US06905825B2
公开(公告)日：2005-06-14
申请号：US10020906
申请日：2001-12-19
申请人： Kyoko Kojima , Satoshi Ozawa
发明人： Kyoko Kojima , Satoshi Ozawa
IPC分类号： G01N30/26 , B01J20/283 , C07H21/00 , C12N15/09 , C12N15/10 , G01N30/88 , C12Q1/68 , C07H21/04 , C12N1/20 , C12N15/00 , C12N15/63
CPC分类号： C12N15/1006
摘要： A method for isolating and purifying nucleic acids with an improved recovery yield is provided. A mixed solution containing the nucleic acids, salts, and an organic solvent is contacted with an adsorption support to cause the nucleic acids to be adsorbed on the support. Then, the nucleic acids are desorbed from the support using an elution buffer. At least one compound containing 2 to 10 carbon atoms as selected from the group consisting of aliphatic ether, aliphatic ester, and aliphatic ketone is used as the organic solvent. The method improves the yield of nucleic acids collection, is easy to implement and less susceptible to contamination.
摘要翻译：提供了一种分离和纯化具有提高的回收率的核酸的方法。将含有核酸，盐和有机溶剂的混合溶液与吸附载体接触以使核酸被吸附在载体上。然后，使用洗脱缓冲液将核酸从支持物解吸。使用至少一种选自脂族醚，脂族酯和脂族酮的含有2至10个碳原子的化合物作为有机溶剂。该方法提高了核酸收集的产量，易于实施，不易受污染。

2. 发明授权

US07241572B2 Method for isolating and purifying nucleic acids 有权
标题翻译：分离和纯化核酸的方法
公开(公告)号：US07241572B2
公开(公告)日：2007-07-10
申请号：US10790231
申请日：2004-03-02
申请人： Kyoko Kojima , Satoshi Ozawa
发明人： Kyoko Kojima , Satoshi Ozawa
IPC分类号： C12Q1/68 , C12N15/00
CPC分类号： C12N15/1006
摘要： A method for isolating and purifying nucleic acids with an improved recovery yield is provided. A mixed solution containing the nucleic acids, salts, and an organic solvent is contacted with an adsorption support to cause the nucleic acids to be adsorbed on the support. Then, the nucleic acids are desorbed from the support using an elution bugger. At least one compound containing 2 to 10 carbon atoms as selected from the group consisting of aliphatic ether, aliphatic ester, and aliphatic ketone is used as the organic solvent. The method improves the yield of nucleic acids collection, is easy to implement and less susceptible to contamination.
摘要翻译：提供了一种分离和纯化具有提高的回收率的核酸的方法。将含有核酸，盐和有机溶剂的混合溶液与吸附载体接触以使核酸被吸附在载体上。然后，使用洗脱剂将核酸从支持物解吸。使用至少一种选自脂族醚，脂族酯和脂族酮的含有2至10个碳原子的化合物作为有机溶剂。该方法提高了核酸收集的产量，易于实施，不易受污染。

3. 发明授权

US09232883B2 Endoscope apparatus 有权
标题翻译：内窥镜装置
公开(公告)号：US09232883B2
公开(公告)日：2016-01-12
申请号：US13017349
申请日：2011-01-31
申请人： Satoshi Ozawa , Takayuki Iida , Yasuhiro Minetoma , Akihiko Erikawa
发明人： Satoshi Ozawa , Takayuki Iida , Yasuhiro Minetoma , Akihiko Erikawa
IPC分类号： A61B1/06 , A61B1/00 , A61B1/04 , A61B1/07
CPC分类号： A61B1/00188 , A61B1/043 , A61B1/063 , A61B1/0638 , A61B1/0653 , A61B1/0684 , A61B1/07
摘要： To provide an endoscope apparatus in which the observation image can be varied continuously as the observation magnification is varied by a zoom magnification varying manipulation so that an observation image suitable for an endoscope diagnosis is obtained at each observation magnification, and to thereby prevent the operator from feeling uncomfortable and increase the accuracy of a diagnosis. An endoscope apparatus is equipped with illuminating unit having plural light sources which generate light beams having different spectra, for illuminating an observation subject; imaging unit for imaging the observation subject; observation magnification varying unit for varying observation magnification of the imaging of the imaging unit; and light quantity ratio varying unit for continuously varying an emission light quantity ratio between the plural light sources according to the observation magnification that is set by the observation magnification varying unit.
摘要翻译：为了提供一种内窥镜装置，其中，随着观察倍率通过变焦倍率变化操作而变化，观察图像可以连续变化，从而在每个观察放大倍数下获得适合于内窥镜诊断的观察图像，从而防止操作者感觉不舒服，提高了诊断的准确性。一种内窥镜装置具备：具有多个光源的照明单元，该光源产生具有不同光谱的光束，用于照明观察对象物; 用于对观察对象成像的成像单元; 用于改变所述成像单元的成像的观察倍率的观察倍率变化单元; 以及光量比变化单元，用于根据由观察倍率变化单元设定的观察倍率连续地改变多个光源之间的发射光量比。

4. 发明授权

US08936548B2 Method of controlling endoscope and endoscope 有权
标题翻译：内窥镜和内窥镜控制方法
公开(公告)号：US08936548B2
公开(公告)日：2015-01-20
申请号：US12890357
申请日：2010-09-24
申请人： Satoshi Ozawa , Akihiko Erikawa , Azuchi Endo
发明人： Satoshi Ozawa , Akihiko Erikawa , Azuchi Endo
IPC分类号： A61B1/06 , A61B6/00 , A61B1/05
CPC分类号： A61B1/0638 , A61B1/05 , A61B1/063 , A61B1/0646 , A61B1/0653
摘要： An endoscope 100 includes a first light source 45 that emits white illumination light, a second light source 47 that emits narrow-band light and an imaging section that has an imaging device 21 having plural detection pixels and images a region to be observed. The imaging section is caused to output a captured image signal including both a return light component of the white illumination light from the region to be observed by and a return light component of the narrow-band light the white illumination light. From the captured image signal, the return light component of the narrow-band light is selectively extracted, and a brightness level of the extracted return light component of the narrow-band light is changed by changing a light amount of light emitted from the second light source 47.
摘要翻译：内窥镜100包括发射白色照明光的第一光源45，发射窄带光的第二光源47和具有多个检测像素的成像装置21和要观察的区域的图像的摄像部。使得成像部分输出包括来自待观察区域的白色照明光的返回光分量和窄带光的白光照明光的返回光分量的捕获图像信号。从拍摄图像信号中，选择性地提取窄带光的返回光分量，并且通过改变从第二光发射的光的光量来改变所提取的窄带光的返回光分量的亮度水平来源47。

5. 发明申请

US20120225448A1 Substrate with Photo-Controllable Cell Adhesion Property, Method for Analyzing and Fractionating Cells, and Device for Analysis and Fractionation of Cells 审中-公开
标题翻译：具有光可控细胞粘附性质的基质，分析细胞分离方法和分离细胞分离装置
公开(公告)号：US20120225448A1
公开(公告)日：2012-09-06
申请号：US13509338
申请日：2010-11-04
申请人： Hisashi Sugiyama , Satoshi Takahashi , Kenko Uchida , Satoshi Ozawa
发明人： Hisashi Sugiyama , Satoshi Takahashi , Kenko Uchida , Satoshi Ozawa
IPC分类号： C12Q1/02 , C12M1/34
CPC分类号： C12M47/04 , C12N1/02 , C12N11/02
摘要： When cells are analyzed, fractionated, and incubated while keeping the cells alive, real-time operations can be performed more easily and the cells can be incubated while removing unnecessary cells from the incubated cells to purify the cells being incubated. Furthermore, desired cells are separated through analysis from the incubated cells, and the purity, recovery, and viability of the cells are heightened. Use is made of a substrate having photo-controllable cell adhesion properties, the substrate comprising a transparent base and, formed thereon, a film of a material which has photo-controllable cell adhesion properties and has been obtained by bonding a cell-adhesive material to a cell-non-adhesive material through photo-dissociable groups. Cell images are detected and analyzed to obtain information about the location of desired cells. On the basis of the information, a space is formed between cells and the material having photo-controllable cell adhesion properties is cut, by means of second light irradiation. Meanwhile, by means of first light irradiation, the surface of the substrate is changed from a cell-adhesive surface to a cell-non-adhesive surface, thereby separating the cell(s) from the substrate. Thus, cells can be analyzed and fractionated while keeping the cells alive.
摘要翻译：当细胞分析，分级和孵育同时保持细胞活着时，可以更容易地进行实时操作，并且可以孵育细胞，同时从培养的细胞中除去不需要的细胞以纯化待孵育的细胞。此外，通过从培养的细胞的分析分离所需的细胞，并且提高细胞的纯度，回收率和存活力。使用具有光可控细胞粘附性质的基材，所述基材包括透明基材，并且在其上形成具有光可控细胞粘附性的材料的膜，并且通过将细胞粘合剂材料粘合到通过光解离基团的细胞非粘合材料。检测和分析细胞图像以获得关于所需细胞位置的信息。基于该信息，在单元之间形成空间，通过第二次光照射切断具有光可控细胞粘附性的材料。同时，通过第一光照射，将基板的表面从电池 - 粘合剂表面改变为电池非粘合剂表面，从而将电池与基板分离。因此，可以在保持细胞活着的同时分析和分级细胞。

6. 发明授权

US07927886B2 Hazardous substance removing method, hazardous substance removing material used therein such as air filter, mask, wipe sheet, and the like, and storage method thereof 有权
标题翻译：有害物质去除方法，其中使用的危险物质去除材料如空气过滤器，掩模，擦拭纸等，及其储存方法
公开(公告)号：US07927886B2
公开(公告)日：2011-04-19
申请号：US12275500
申请日：2008-11-21
申请人： Satoshi Ozawa , Jun-ichiro Arai
发明人： Satoshi Ozawa , Jun-ichiro Arai
IPC分类号： G01N33/543
CPC分类号： D06M16/003 , A61L9/014 , A61L9/16
摘要： A hazardous substance (20) is removed by using a hazardous substance removing material (10) in which a support (11) supports an antibody (12). Humidity of the ambient atmosphere of the antibody (12) is controlled so that the antibody (12) becomes active.
摘要翻译：通过使用其中载体（11）支持抗体（12）的有害物质去除物质（10）除去有害物质（20）。控制抗体（12）的环境气氛的湿度，使得抗体（12）变得活性。

7. 发明授权

US07754478B2 Device for cell culture 有权
标题翻译：细胞培养装置
公开(公告)号：US07754478B2
公开(公告)日：2010-07-13
申请号：US10583511
申请日：2004-12-15
申请人： Tsutomu Suzuki , Hiroshi Inomata , Yoshihiro Komori , Hiroshi Tachikui , Naruo Watanabe , Satoshi Ozawa , Yuji Oka , Minoru Ueda , Yasushi Nomura , Masako Nomura, legal representative , Isao Shindo
发明人： Tsutomu Suzuki , Hiroshi Inomata , Yoshihiro Komori , Hiroshi Tachikui , Naruo Watanabe , Satoshi Ozawa , Yuji Oka , Minoru Ueda , Yasushi Nomura , Isao Shindo
IPC分类号： C12M1/02 , C12M1/34
CPC分类号： C12M41/14 , C12M23/22 , C12M27/10 , C12M41/48 , Y10S435/809
摘要： A device for cell culture capable of automatically performing operations for cell culture over several days to several months while minimizing the risk of contamination. A new medicine can be supplied to an incubator means by using a medicine supply means or unnecessary wastewater can be discharged from the incubator means by using a wastewater discharge means without taking out the incubator means disposed in heat insulation box means from a heat insulation box, and the state of the cell culture can be observed with the incubator means formed in the heat insulation box means. Accordingly, the outside air does not enter directly into the incubator means during culturing, and the risk of contamination is completely eliminated. As a result, the culturing operations can be automatically performed over a long period.
摘要翻译：用于细胞培养的装置，其能够在几天至几个月内自动执行细胞培养的操作，同时最小化污染的风险。可以通过使用药物供给装置向培养箱装置提供新药，或者可以通过使用废水排放装置将不需要的废水从培养箱装置排出，而不从隔热箱取出设置在隔热箱装置中的培养箱装置，并且可以用在隔热箱装置中形成的培养箱装置来观察细胞培养的状态。因此，外部空气在培养期间不直接进入培养箱装置，并且完全消除污染的风险。结果，可以长时间自动进行培养操作。

8. 发明申请

US20100001047A1 WAVE SOLDERING TANK 有权
标题翻译： WAVE SOLDERING坦克
公开(公告)号：US20100001047A1
公开(公告)日：2010-01-07
申请号：US12225925
申请日：2007-04-02
申请人： Mitsuo Zen , Hirokazu Ichikawa , Satoshi Ozawa
发明人： Mitsuo Zen , Hirokazu Ichikawa , Satoshi Ozawa
IPC分类号： B23K31/02
CPC分类号： B23K3/0653 , B23K1/0016 , B23K1/085 , B23K2101/42 , H05K3/3468
摘要： In a wave soldering tank using a conventional screw pump, pulsation sometimes occurred in which molten solder spouting from the discharge nozzle moved up and down. The cause of the occurrence of the pulsation in a screw pump was that the gap between the screw pump and the casing was wide, so there was reverse flow through the gap. It is conceivable to narrow the gap, but if the gap is made narrow, the screw pump ends up contacting the casing if the screw pump becomes eccentric.A wave soldering tank according to the present invention makes the diameter of the inlet in the bottom of the casing smaller than the diameter of the screw pump so that reverse flow does not take place even if the gap between the screw pump and the casing is wide. Flow is made more stable by providing outwardly flaring guide walls on the outlet of the casing.
摘要翻译：在使用常规螺杆泵的波峰焊槽中，有时会发生从排出喷嘴喷出的熔融焊料上下移动的脉动。螺杆泵中发生脉动的原因是螺杆泵与壳体之间的间隙较宽，因此通过间隙有反向流动。可以想到缩小间隙，但是如果间隙变窄，则如果螺杆泵变得偏心，则螺杆泵最终接触壳体。根据本发明的波峰焊槽使得壳体底部的入口的直径小于螺杆泵的直径，使得即使螺杆泵和壳体之间的间隙较宽，也不会发生反向流动。通过在外壳的出口上提供向外扩张的导向壁，使流动更加稳定。

9. 发明授权

US07515244B2 Print production system 有权
标题翻译：打印生产系统
公开(公告)号：US07515244B2
公开(公告)日：2009-04-07
申请号：US11395307
申请日：2006-04-03
申请人： Satoshi Ozawa
发明人： Satoshi Ozawa
IPC分类号： G03B27/52 , G06F3/12
CPC分类号： G03B27/52 , G03B2206/00 , G06Q30/00 , H04N1/00132 , H04N1/00137 , H04N1/00143 , H04N1/00167 , H04N1/00188
摘要： The print production system includes at least one order receiver including an image acquisition unit for acquiring first image data, an input unit for inputting a print order of a second image data in the first image data and a reception processing unit outputting order information and second image data related to each other by the reception processing unit, and an image forming apparatus including an image processing unit for performing image processing on the second image data to create third image data, and a print production unit for producing prints of the third image data. At least one of transfer of the second image data from the reception processing unit to the image processing unit and transfer of the third image data from the image processing unit to the print production unit is performed such that image data of images of a predetermined number of frames are transferred at a time.
摘要翻译：印刷生产系统包括至少一个订单接收器，包括用于获取第一图像数据的图像获取单元，用于输入第一图像数据中的第二图像数据的打印顺序的输入单元和输出订单信息和第二图像的接收处理单元由接收处理单元相关的数据以及包括用于对第二图像数据执行图像处理以创建第三图像数据的图像处理单元的图像形成装置，以及用于产生第三图像数据的打印的打印生成单元。执行将第二图像数据从接收处理单元传送到图像处理单元并将第三图像数据从图像处理单元传送到打印制作单元中的至少一个，使得预定数量的图像数据的图像数据帧一次传送。

10. 发明申请

US20090078263A1 Hazardous substance removing method, hazardous substance removing material used therein such as air filter, mask, wipe sheet, and the like, and storage method thereof 有权
标题翻译：有害物质去除方法，其中使用的危险物质去除材料如空气过滤器，掩模，擦拭纸等，及其储存方法
公开(公告)号：US20090078263A1
公开(公告)日：2009-03-26
申请号：US12275500
申请日：2008-11-21
申请人： Satoshi Ozawa , Jun-ichiro Araj
发明人： Satoshi Ozawa , Jun-ichiro Araj
IPC分类号： A62B7/10
CPC分类号： D06M16/003 , A61L9/014 , A61L9/16
摘要： A hazardous substance (20) is removed by using a hazardous substance removing material (10) in which a support (11) supports an antibody (12). Humidity of the ambient atmosphere of the antibody (12) is controlled so that the antibody (12) becomes active.
摘要翻译：通过使用其中载体（11）支持抗体（12）的有害物质去除物质（10）除去有害物质（20）。控制抗体（12）的环境气氛的湿度，使得抗体（12）变得活性。

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