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1. 发明申请

US20100311063A1 METHODS AND COMPOSITIONS FOR LABELING NUCLEIC ACIDS 有权
标题翻译：标记核酸的方法和组合物
公开(公告)号：US20100311063A1
公开(公告)日：2010-12-09
申请号：US12785999
申请日：2010-05-24
申请人： Kyle R. Gee , Brian Agnew , Adrian Salic , Timothy J. Mitchison
发明人： Kyle R. Gee , Brian Agnew , Adrian Salic , Timothy J. Mitchison
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/68 , C12Q1/6816 , C12Q1/6841 , G01N33/5011 , G01N33/582 , C12Q2525/101 , C12Q2563/107
摘要： The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.
摘要翻译：本发明涉及体外和体内标记核酸聚合物的方法。提供了包括掺入核酸聚合物的核苷酸类似物与附着于标记物上的试剂之间的[3 + 2]环加成的某些方法。提供了包括掺入核酸聚合物的核苷酸类似物与包含连接到标签上的取代的三芳基膦的试剂之间的施陶丁格连接的其它方法。这种方法不需要固定和变性，因此可以应用于活细胞和生物体中核酸聚合物的标记。还提供了测量细胞增殖的方法。在这些方法中，测量结合到DNA中的标记量作为细胞增殖的指示。本发明的方法可用于各种应用，包括涉及细胞增殖的疾病和病症的临床诊断，毒性测定以及用于研究染色体超微结构的工具。

2. 发明授权

US07767421B2 Methods and compositions for labeling nucleic acids 有权
标题翻译：用于标记核酸的方法和组合物
公开(公告)号：US07767421B2
公开(公告)日：2010-08-03
申请号：US11588697
申请日：2006-10-27
申请人： Kyle R. Gee , Brian Agnew , Adrian Salic , Timothy J. Mitchison
发明人： Kyle R. Gee , Brian Agnew , Adrian Salic , Timothy J. Mitchison
IPC分类号： C12P19/34 , C07H21/02
CPC分类号： C12Q1/6806 , C12Q1/68 , C12Q1/6816 , C12Q1/6841 , G01N33/5011 , G01N33/582 , C12Q2525/101 , C12Q2563/107
摘要： The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.
摘要翻译：本发明涉及体外和体内标记核酸聚合物的方法。提供了包括掺入核酸聚合物的核苷酸类似物与附着于标记物上的试剂之间的[3 + 2]环加成的某些方法。提供了包括掺入核酸聚合物的核苷酸类似物与包含连接到标签上的取代的三芳基膦的试剂之间的施陶丁格连接的其它方法。这种方法不需要固定和变性，因此可以应用于活细胞和生物体中核酸聚合物的标记。还提供了测量细胞增殖的方法。在这些方法中，测量结合到DNA中的标记量作为细胞增殖的指示。本发明的方法可用于各种应用，包括涉及细胞增殖的疾病和病症的临床诊断，毒性测定以及用于研究染色体超微结构的工具。

3. 发明授权

US08541570B2 Methods and compositions for labeling nucleic acids 有权
标题翻译：用于标记核酸的方法和组合物
公开(公告)号：US08541570B2
公开(公告)日：2013-09-24
申请号：US12785999
申请日：2010-05-24
申请人： Kyle R. Gee , Brian Agnew , Adrian Salic , Timothy J. Mitchison
发明人： Kyle R. Gee , Brian Agnew , Adrian Salic , Timothy J. Mitchison
IPC分类号： C07H19/06 , C07F9/02 , G01N33/48
CPC分类号： C12Q1/6806 , C12Q1/68 , C12Q1/6816 , C12Q1/6841 , G01N33/5011 , G01N33/582 , C12Q2525/101 , C12Q2563/107
摘要： The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.
摘要翻译：本发明涉及体外和体内标记核酸聚合物的方法。提供了包括掺入核酸聚合物的核苷酸类似物与附着于标记物上的试剂之间的[3 + 2]环加成的某些方法。提供了包括掺入核酸聚合物的核苷酸类似物与包含连接到标签上的取代的三芳基膦的试剂之间的施陶丁格连接的其它方法。这种方法不需要固定和变性，因此可以应用于活细胞和生物体中核酸聚合物的标记。还提供了测量细胞增殖的方法。在这些方法中，测量结合到DNA中的标记量作为细胞增殖的指示。本发明的方法可用于各种应用，包括涉及细胞增殖的疾病和病症的临床诊断，毒性测定以及用于研究染色体超微结构的工具。

4. 发明申请

US20100261181A1 LABELING AND DETECTION OF NUCLEIC ACIDS 审中-公开
标题翻译：标记和检测核酸
公开(公告)号：US20100261181A1
公开(公告)日：2010-10-14
申请号：US12729903
申请日：2010-03-23
申请人： Brian Agnew , Maura J. Ford , Kyle R. Gee , Kapil Kumar
发明人： Brian Agnew , Maura J. Ford , Kyle R. Gee , Kapil Kumar
IPC分类号： C12Q1/68 , C12P19/34 , G01N33/48 , C07H21/00 , C12N9/12 , C12N9/00
CPC分类号： C12Q1/6806 , C07D207/40 , C07D263/32 , C07D295/22 , C07D307/58 , C07D311/88 , C09B11/24 , C12Q1/68 , C12Q1/6841 , C12Q1/686 , G01N33/5308 , G01N33/531 , G01N2333/91245 , Y10T436/143333
摘要： Provided in certain embodiments are new methods for forming azido modified nucleic acid conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling nucleic acids with an azide group.
摘要翻译：在某些实施方案中提供了形成报道分子，载体分子或固体支持物的叠氮基修饰的核酸缀合物的新方法。在其它实施方案中提供了用叠氮化物基团酶标记核酸的方法。

5. 发明授权

US08716033B2 Oligosaccharide modification and labeling of proteins 有权
公开(公告)号：US08716033B2
公开(公告)日：2014-05-06
申请号：US11674136
申请日：2007-02-12
申请人： Brian Agnew , Schuyler B. Corry , Kyle R. Gee
发明人： Brian Agnew , Schuyler B. Corry , Kyle R. Gee
IPC分类号： G01N33/532 , G01N33/534 , G01N33/533 , C07K1/10
CPC分类号： C12P21/005 , A61K47/549 , A61K47/61 , C07K1/13 , C07K14/47 , C07K16/00 , C07K2317/14 , C07K2317/24 , C07K2317/41 , C12Y302/01096 , G01N33/5005 , G01N33/5008 , G01N33/531 , G01N33/532 , G01N33/533 , G01N33/534 , G01N33/581 , G01N33/582 , G01N33/583 , Y10T436/17
摘要： The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention.

6. 发明授权

US08114636B2 Labeling and detection of nucleic acids 有权
标题翻译：核酸的标记和检测
公开(公告)号：US08114636B2
公开(公告)日：2012-02-14
申请号：US11674623
申请日：2007-02-13
申请人： Brian Agnew , Maura J. Ford , Kyle R. Gee , Kapil Kumar
发明人： Brian Agnew , Maura J. Ford , Kyle R. Gee , Kapil Kumar
IPC分类号： C12P19/34 , C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6806 , C07D207/40 , C07D263/32 , C07D295/22 , C07D307/58 , C07D311/88 , C09B11/24 , C12Q1/68 , C12Q1/6841 , C12Q1/686 , G01N33/5308 , G01N33/531 , G01N2333/91245 , Y10T436/143333
摘要： Provided in certain embodiments are new methods for forming azido modified nucleic acid conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling nucleic acids with an azide group.
摘要翻译：在某些实施方案中提供了形成报道分子，载体分子或固体支持物的叠氮基修饰的核酸缀合物的新方法。在其它实施方案中提供了用叠氮化物基团酶标记核酸的方法。

7. 发明授权

US07776533B2 Compositions and methods for detection and isolation of phosphorylated molecules 有权
公开(公告)号：US07776533B2
公开(公告)日：2010-08-17
申请号：US11552275
申请日：2006-10-24
申请人： Brian Agnew , Joseph Beechem , Kyle R Gee , Richard P Haugland , Thomas H Steinberg , Wayne F Patton
发明人： Brian Agnew , Joseph Beechem , Kyle R Gee , Richard P Haugland , Thomas H Steinberg , Wayne F Patton
IPC分类号： G01N33/53
CPC分类号： G01N33/5008 , C12Q1/42 , C12Q1/485 , G01N33/502 , G01N33/5091 , G01N2333/4709 , Y10T436/142222 , Y10T436/145555 , Y10T436/147777 , Y10T436/201666
摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.

8. 发明申请

US20110207171A1 OLIGOSACCHARIDE MODIFICATION AND LABELING OF PROTEINS 有权
标题翻译：蛋白质的寡糖修饰和标记
公开(公告)号：US20110207171A1
公开(公告)日：2011-08-25
申请号：US13007506
申请日：2011-01-14
申请人： Brian Agnew , Schuyler B. CORRY , Kyle R. GEE
发明人： Brian Agnew , Schuyler B. CORRY , Kyle R. GEE
IPC分类号： C12P33/00 , C12P21/00 , C07K16/00 , C12N9/96
CPC分类号： C12P21/005 , A61K47/549 , A61K47/61 , C07K1/13 , C07K14/47 , C07K16/00 , C07K2317/14 , C07K2317/24 , C07K2317/41 , C12Y302/01096 , G01N33/5005 , G01N33/5008 , G01N33/531 , G01N33/532 , G01N33/533 , G01N33/534 , G01N33/581 , G01N33/582 , G01N33/583 , Y10T436/17
摘要： The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention.
摘要翻译：本发明通常涉及在寡糖键上官能化蛋白质，特别是抗体的方法，通过修饰糖基化来人源化抗体的方法，以及与修饰的寡糖连接的新型抗体。本发明还涉及可用于产生本发明抗体的试剂盒。

9. 发明授权

US07445894B2 Compositions and methods for detection and isolation of phosphorylated molecules 有权
标题翻译：用于磷酸化分子检测和分离的组合物和方法
公开(公告)号：US07445894B2
公开(公告)日：2008-11-04
申请号：US10821522
申请日：2004-04-09
申请人： Brian Agnew , Kyle R. Gee , Vladimir V. Martin
发明人： Brian Agnew , Kyle R. Gee , Vladimir V. Martin
IPC分类号： G01N33/50
CPC分类号： G01N33/5008 , G01N33/502 , G01N33/5091 , G01N2333/4709
摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
摘要翻译：本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在磷酸化结合化合物和导致三元复合物的磷酸化靶分子。本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。

10. 发明授权

US08785212B2 Oligosaccharide modification and labeling of proteins 有权
标题翻译：寡糖修饰和蛋白质标记
公开(公告)号：US08785212B2
公开(公告)日：2014-07-22
申请号：US13007506
申请日：2011-01-14
申请人： Brian Agnew , Kyle Gee , Schuyler Corry
发明人： Brian Agnew , Kyle Gee , Schuyler Corry
IPC分类号： G01N33/532 , G01N33/534 , G01N33/533 , G01N33/50 , A61K47/48
CPC分类号： C12P21/005 , A61K47/549 , A61K47/61 , C07K1/13 , C07K14/47 , C07K16/00 , C07K2317/14 , C07K2317/24 , C07K2317/41 , C12Y302/01096 , G01N33/5005 , G01N33/5008 , G01N33/531 , G01N33/532 , G01N33/533 , G01N33/534 , G01N33/581 , G01N33/582 , G01N33/583 , Y10T436/17
摘要： The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention.
摘要翻译：本发明通常涉及在寡糖键上官能化蛋白质，特别是抗体的方法，通过修饰糖基化来人源化抗体的方法，以及与修饰的寡糖连接的新型抗体。本发明还涉及可用于产生本发明抗体的试剂盒。

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