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1. 发明授权

US07445894B2 Compositions and methods for detection and isolation of phosphorylated molecules 有权
标题翻译：用于磷酸化分子检测和分离的组合物和方法
公开(公告)号：US07445894B2
公开(公告)日：2008-11-04
申请号：US10821522
申请日：2004-04-09
申请人： Brian Agnew , Kyle R. Gee , Vladimir V. Martin
发明人： Brian Agnew , Kyle R. Gee , Vladimir V. Martin
IPC分类号： G01N33/50
CPC分类号： G01N33/5008 , G01N33/502 , G01N33/5091 , G01N2333/4709
摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
摘要翻译：本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在磷酸化结合化合物和导致三元复合物的磷酸化靶分子。本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。

2. 发明授权

US07129346B2 Crown ether derivatives 有权
标题翻译：冠醚衍生物
公开(公告)号：US07129346B2
公开(公告)日：2006-10-31
申请号：US10634336
申请日：2003-08-04
申请人： Kyle R. Gee , Vladimir V. Martin
发明人： Kyle R. Gee , Vladimir V. Martin
IPC分类号： C07D225/00 , C07D267/22
CPC分类号： C07H21/00 , C07D273/00 , C09B57/10 , C09B69/00 , G01N33/582 , G01N33/84
摘要： The invention describes crown ether chelators, including crown ethers having the formula and aza-substituted and thia-substituted analogs thereof. These crown ethers may be substituted by a dye moiety, a chemically reactive group, a conjugated substance, or a combination thereof. Chelators that are substituted by fluorescent dyes are particularly useful as indicators for metal cations, particularly Na+ and K+ ions, and particularly where binding of the target ion results in a change in the fluorescence properties of the indicator that can be correlated with the ion concentration. Methods are provided for utilizing reactive groups on the chelators for conjugation to dyes, lipids and polymers and methods for enhancing entry of the indicators into living cells.
摘要翻译：本发明描述了冠醚螯合剂，包括具有下式的冠醚和其氮杂取代和硫取代的类似物。这些冠醚可以被染料部分，化学反应性基团，共轭物质或其组合取代。被荧光染料取代的螯合剂特别可用作金属阳离子，特别是Na +和K + +离子的指示剂，特别是当目标离子的结合导致变化时在可以与离子浓度相关的指示剂的荧光特性中。提供了利用螯合剂上的反应性基团与染料偶联的方法，脂质和聚合物以及用于增强指示剂进入活细胞的方法。

3. 发明申请

US20100167333A1 Heavy Metal Binding Compounds and Their Method of Use 有权
标题翻译：重金属结合化合物及其使用方法
公开(公告)号：US20100167333A1
公开(公告)日：2010-07-01
申请号：US12636687
申请日：2009-12-11
申请人： Kyle R. Gee , Jolene Bradford , Vladimir V. Martin
发明人： Kyle R. Gee , Jolene Bradford , Vladimir V. Martin
IPC分类号： C12Q1/02 , G01N1/30 , G01N33/20 , C07K2/00 , C07K14/76 , C07K14/435 , C07K16/00 , C07K14/42 , A61K38/18 , C07H21/04 , C08B37/02 , C07D413/04 , C07D209/20 , C07D311/90
CPC分类号： C07D413/04 , C07D209/14 , C07D335/12 , C09B11/28
摘要： The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of heavy metal ions in a sample. This metal chelating moiety is a —N,N,O-triacetic acid analog of BAPTA and has the following formula
摘要翻译：本发明提供金属螯合剂和促进样品中重金属离子结合，检测，监测和定量的方法。该金属螯合部分是BAPTA的-N，N，O-三乙酸类似物，具有下式

4. 发明授权

US07579463B2 Crown ether derivatives 失效
标题翻译：冠醚衍生物
公开(公告)号：US07579463B2
公开(公告)日：2009-08-25
申请号：US11538010
申请日：2006-10-02
申请人： Kyle R. Gee , Vladimir V. Martin
发明人： Kyle R. Gee , Vladimir V. Martin
IPC分类号： C07D225/00 , C07D267/22
CPC分类号： C07D273/00 , C07D413/04 , C07D413/14
摘要： The invention describes crown ether chelators, including crown ethers having the formula:
摘要翻译：本发明描述了冠醚螯合剂，包括具有下式的冠醚：

5. 发明申请

US20100047821A1 CROWN ETHER DERIVATIVES 有权
标题翻译： CROWN ETHER DORIVATIVES
公开(公告)号：US20100047821A1
公开(公告)日：2010-02-25
申请号：US12546179
申请日：2009-08-24
申请人： Kyle R. Gee , Vladimir V. Martin
发明人： Kyle R. Gee , Vladimir V. Martin
IPC分类号： G01N33/53 , C07D273/00
CPC分类号： C07D273/00 , C07D413/04 , C07D413/14
摘要： The invention describes crown ether chelators, including crown ethers having the formula:
摘要翻译：本发明描述了冠醚螯合剂，包括具有下式的冠醚：

6. 发明授权

US07521577B2 Heavy metal binding compounds and their method of use 有权
标题翻译：重金属结合化合物及其使用方法
公开(公告)号：US07521577B2
公开(公告)日：2009-04-21
申请号：US11622900
申请日：2007-01-12
申请人： Kyle R. Gee , Jolene Bradford , Vladimir V. Martin
发明人： Kyle R. Gee , Jolene Bradford , Vladimir V. Martin
IPC分类号： C07C229/00 , C07C321/00 , C07C205/00
CPC分类号： C07D413/04 , C07D209/14 , C07D335/12 , C09B11/28
摘要： The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of heavy metal ions in a sample. This metal chelating moiety is a —N,N,O-triacetic acid analog of BAPTA and has the following formula
摘要翻译：本发明提供金属螯合剂和促进样品中重金属离子结合，检测，监测和定量的方法。该金属螯合部分是BAPTA的-N，N，O-三乙酸类似物，具有下式

7. 发明授权

US06962992B2 Crown ether derivatives 失效
标题翻译：冠醚衍生物
公开(公告)号：US06962992B2
公开(公告)日：2005-11-08
申请号：US10026302
申请日：2001-12-19
申请人： Vladimir V. Martin , Kyle R. Gee , Richard P. Haugland , Zhenjun Diwu
发明人： Vladimir V. Martin , Kyle R. Gee , Richard P. Haugland , Zhenjun Diwu
IPC分类号： C07D273/00 , C07H21/00 , C09B57/10 , C09B69/00 , G01N33/58 , G01N33/84 , C07D413/04 , C07D273/08 , C07D317/28
CPC分类号： A61K49/0052 , A61K49/0021 , A61K49/0041 , C07D273/00 , C07H21/00 , C09B11/24 , C09B23/04 , C09B57/00 , C09B57/10 , C09B69/00 , G01N33/582 , G01N33/84
摘要： The invention describes crown ether chelators, including crown ethers having the formula and aza-substituted and thia-substituted analogs thereof. These crown ethers are substituted by a dye moiety, a chemically reactive group, a conjugated substance, or a combination thereof. Chelators that are substituted by fluorescent dyes are particularly useful as indicators for metal cations, particularly Na+ and K+ ions, and particularly where binding of the target ion results in a change in the fluorescence properties of the indicator that can be correlated with the ion concentration. Methods are provided for utilizing reactive groups on the chelators for conjugation to dyes, lipids and polymers and methods for enhancing entry of the indicators into living cells.
摘要翻译：本发明描述了冠醚螯合剂，包括具有下式的冠醚和其氮杂取代和硫取代的类似物。这些冠醚被染料部分，化学反应性基团，共轭物质或其组合取代。被荧光染料取代的螯合剂特别可用作金属阳离子，特别是Na +和K + +离子的指示剂，特别是当目标离子的结合导致变化时在可以与离子浓度相关的指示剂的荧光特性中。提供了利用螯合剂上的反应性基团与染料偶联的方法，脂质和聚合物以及用于增强指示剂进入活细胞的方法。

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