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1. 发明申请

US20090208941A1 Method for investigating cytosine methylations in dna 审中-公开
标题翻译：在dna中调查胞嘧啶甲基化的方法
公开(公告)号：US20090208941A1
公开(公告)日：2009-08-20
申请号：US11989081
申请日：2006-07-19
申请人： Kurt Berlin , Matthias Schuster , Philipp Schatz
发明人： Kurt Berlin , Matthias Schuster , Philipp Schatz
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827
摘要： A method for sensitive and specific detection of cytosine methylation is described wherein the DNA to be analyzed is subjected to initial enrichment. More specifically, said enrichment can be effected in a methylation-specific, sequence-specific or origin-specific fashion. Thereafter, the enriched DNA is converted in a methylation-specific fashion. The converted DNA can be analyzed using various methods, particularly real-time PCR methods.
摘要翻译：描述了用于敏感和特异性检测胞嘧啶甲基化的方法，其中待分析的DNA经受初始富集。更具体地，所述富集可以以甲基化特异性，序列特异性或起始特异性方式实现。此后，富集的DNA以甲基化特异性方式转化。可以使用各种方法，特别是实时PCR方法分析转化的DNA。

2. 发明申请

US20060166201A1 Method for the detection of nucleic acid sequences by means of crackable probe molecules 审中-公开
标题翻译：通过可裂解的探针分子检测核酸序列的方法
公开(公告)号：US20060166201A1
公开(公告)日：2006-07-27
申请号：US10526277
申请日：2003-09-01
申请人： Philipp Schatz , Matthias Schuster , Kurt Berlin
发明人： Philipp Schatz , Matthias Schuster , Kurt Berlin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q2563/167
摘要： The present invention describes a method for the detection of nucleic acid sequences, which is characterized in that the following steps are conducted: a) at least one nucleic acid is bound to a solid phase; b) probe molecules are hybridized to the nucleic acids in a sequence-specific manner, whereby the probe molecules are provided with a cleavable bond and a mass label, which is specific for the probe molecule; c) removal of the unhybridized probe molecules; d) contacting of the hybridized probe molecules with a matrix, which cleaves said cleavable bonds and at the same time serves as the matrix in a MALDI mass spectrometer; e) detection of the mass label at those positions where the nucleic acid was bound.
摘要翻译：本发明描述了用于检测核酸序列的方法，其特征在于进行以下步骤：a）至少一种核酸与固相结合; b）探针分子以序列特异性方式与核酸杂交，由此提供探针分子具有可切割键和对于探针分子是特异性的质量标记; c）去除未杂交的探针分子; d）将杂交的探针分子与基质接触，所述基质切割所述可切割键，并同时用作MALDI质谱仪中的基质; e）在核酸结合的那些位置检测质量标签。

3. 发明申请

US20080124735A1 Method for detection of one or more CpG positions 审中-公开
标题翻译：用于检测一个或多个CpG位置的方法
公开(公告)号：US20080124735A1
公开(公告)日：2008-05-29
申请号：US11974919
申请日：2007-10-16
申请人： Matthias Schuster , Philipp Schatz , Esmeralda Heiden
发明人： Matthias Schuster , Philipp Schatz , Esmeralda Heiden
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q2537/164
摘要： The invention relates generally to novel and substantially improved methods for detecting CpG positions. Particular aspects relate to a method for detection of one or more CpG positions. Said method comprises: providing a sample, binding a protein or peptide onto the DNA of said sample, hybridizing a probe onto the DNA of said sample, detecting a signal determined by said bound proteins or peptides and by said hybridized probes. Thereby one or more CpG positions are detected in a methylation specific and sequence specific manner.
摘要翻译：本发明一般涉及用于检测CpG位置的新颖且显着改进的方法。特定方面涉及用于检测一个或多个CpG位置的方法。所述方法包括：提供样品，将蛋白质或肽结合到所述样品的DNA上，将探针与所述样品的DNA杂交，检测由所述结合的蛋白质或肽和所述杂交探针确定的信号。从而以甲基化特异性和序列特异性方式检测一个或多个CpG位置。

4. 发明申请

US20090004646A1 Method for Quantifying Methylated Dna 审中-公开
标题翻译：甲基化Dna的定量方法
公开(公告)号：US20090004646A1
公开(公告)日：2009-01-01
申请号：US11629743
申请日：2005-06-15
申请人： Matthias Schuster , Philipp Schatz
发明人： Matthias Schuster , Philipp Schatz
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2537/164 , C12Q2521/331
摘要： The inventive method makes it possible to quantify methylated DNA by combining the restricted digestion and real-time PCR. For this purpose, the inventive method consists in isolating the examined DNA from a biological sample, in reacting the isolated AND with a methylation specific restriction enzyme, in amplifying by means of a real-time PCR, wherein the amplified products are formed only when the DNA is precut-off and, afterwards, in calculated the methylated and unmethylated DNA proportion in the initial sample with the aid of a reference measurement. Said method is particularly suitable for diagnosis and prognosis cancer and other diseases associated with a methylation state modification and for predicting the drug effects.
摘要翻译：本发明的方法使得可以通过组合限制性消化和实时PCR来定量甲基化DNA。为此目的，本发明的方法在于通过实时PCR扩增从分离出的生物样品中的DNA，使分离的AND与甲基化特异性限制酶反应，其中扩增产物仅在 DNA被去除，然后借助于参考测量计算初始样品中的甲基化和非甲基化DNA比例。所述方法特别适用于与甲基化状态修饰相关的癌症和其他疾病的诊断和预后，并用于预测药物作用。

5. 发明申请

US20090075251A1 Method for analysis of cytosine methylation 审中-公开
标题翻译：胞嘧啶甲基化分析方法
公开(公告)号：US20090075251A1
公开(公告)日：2009-03-19
申请号：US10594013
申请日：2005-03-24
申请人： Dimo Dietrich , Philipp Schatz , Matthias Schuster , Antje Kluth
发明人： Dimo Dietrich , Philipp Schatz , Matthias Schuster , Antje Kluth
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q2525/143 , C12Q2523/125
摘要： A method for the analysis of cytosine methylations in DNA is described. Here, the DNA to be investigated is first chemically or enzymatically converted. Then a promoter is introduced into the DNA. After this, the DNA is converted to RNA. The methylation pattern of the DNA can be concluded in different ways by means of an analysis of the RNA. The RNA is preferably fragmented chemically or enzymatically prior to the analysis, whereby the fragmenting results depend on the methylation pattern of the DNA. The method according to the invention is particularly suitable for the diagnosis and prognosis of cancer disorders and other diseases associated with a modification of the methylation pattern.
摘要翻译：描述了分析DNA中胞嘧啶甲基化的方法。这里，待研究的DNA首先被化学或酶转化。然后将启动子引入DNA。之后，将DNA转化为RNA。 DNA的甲基化模式可以通过RNA的分析以不同的方式得出结论。优选在分析之前将RNA片段化学或酶促，由此片段化结果取决于DNA的甲基化模式。根据本发明的方法特别适合于与甲基化模式的改变相关的癌症病症和其它疾病的诊断和预后。

6. 发明申请

US20110059432A1 METHOD FOR PROVIDING DNA FRAGMENTS DERIVED FROM A REMOTE SAMPLE 审中-公开
标题翻译：用于提供从远程样品衍生的DNA片段的方法
公开(公告)号：US20110059432A1
公开(公告)日：2011-03-10
申请号：US11910887
申请日：2006-04-17
申请人： Matthias Ballhause , Kurt Berlin , Theo De Vos , Dimo Dietrich , Volker Liebenberg , Catherine Lofton-Day , Joe Lograsso , Jennifer Maas , Fabian Model , Matthias Schuster , Andrew Z. Sledziewski , Reimo Tetzner
发明人： Matthias Ballhause , Kurt Berlin , Theo De Vos , Dimo Dietrich , Volker Liebenberg , Catherine Lofton-Day , Joe Lograsso , Jennifer Maas , Fabian Model , Matthias Schuster , Andrew Z. Sledziewski , Reimo Tetzner
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34 , G01N33/48
摘要： Aspects of the present invention relate to compositions and methods for providing DNA fragments from a remote sample. In particular aspects a remote sample comprising DNA is provided, DNA is isolated from the remote sample, and the isolated DNA is treated in a way which allows differentiation of methylated and unmethylated cytosine. Additional, particular embodiments provide compositions and methods for methylation analysis of DNA derived from a remote sample. Other aspects provide for compositions and methods of whole genome amplification of bisulfite treated DNA.
摘要翻译：本发明的方面涉及从远程样品提供DNA片段的组合物和方法。在特定方面，提供了包含DNA的远程样品，从远程样品中分离DNA，并以允许甲基化和非甲基化胞嘧啶分化的方式处理分离的DNA。另外，具体实施方案提供了用于从远程样品衍生的DNA的甲基化分析的组合物和方法。其他方面提供了亚硫酸氢盐处理的DNA的全基因组扩增的组合物和方法。

7. 发明授权

US10731215B2 Method for determining the presence or absence of methylation in a sample 有权
公开(公告)号：US10731215B2
公开(公告)日：2020-08-04
申请号：US11910887
申请日：2006-04-17
申请人： Matthias Ballhause , Kurt Berlin , Theo De Vos , Dimo Dietrich , Volker Liebenberg , Catherine Lofton-Day , Joe Lograsso , Jennifer Maas , Fabian Model , Matthias Schuster , Andrew Z. Sledziewski , Reimo Tetzner
发明人： Matthias Ballhause , Kurt Berlin , Theo De Vos , Dimo Dietrich , Volker Liebenberg , Catherine Lofton-Day , Joe Lograsso , Jennifer Maas , Fabian Model , Matthias Schuster , Andrew Z. Sledziewski , Reimo Tetzner
IPC分类号： C12Q1/68 , C12Q1/6883 , C12N15/10 , C12Q1/6806 , C07H21/04
摘要： Aspects of the present invention relate to compositions and methods for providing DNA fragments from a remote sample. In particular aspects a remote sample comprising DNA is provided, DNA is isolated from the remote sample, and the isolated DNA is treated in a way which allows differentiation of methylated and unmethylated cytosine. Additional, particular embodiments provide compositions and methods for methylation analysis of DNA derived from a remote sample. Other aspects provide for compositions and methods of whole genome amplification of bisulfite treated DNA. Other aspects provide methods for determining the presence or absence of methylation of at least one cytosine, or a series of cytosines in cis, in human DNA of a blood sample, a plasma sample, a serum sample or a urine sample from a human individual.

8. 发明申请

US20080220418A1 Method For Providing Dna Fragments Derived From An Archived Sample 有权
标题翻译：提供从存档样本派生的Dna片段的方法
公开(公告)号：US20080220418A1
公开(公告)日：2008-09-11
申请号：US11664367
申请日：2005-09-30
申请人： Matthias Ballhause , Kurt Berlin , Dimo Dietrich , Antje Lukas , Matthias Schuster , Ute Wagner , Reinhold Wasserkort , Heike Ziebarth
发明人： Matthias Ballhause , Kurt Berlin , Dimo Dietrich , Antje Lukas , Matthias Schuster , Ute Wagner , Reinhold Wasserkort , Heike Ziebarth
IPC分类号： C12Q1/68 , C07H1/00 , C12P19/34
CPC分类号： C12Q1/6806 , C12N15/1003 , C12Q1/6858 , C12Q1/686 , C12Q2523/125 , C12Q2527/137 , C12Q2527/149 , C12Q2531/107
摘要： Aspects of the present invention relate to compositions and methods for providing DNA fragments from an archived sample (e.g., paraffin-embedded and/or fixed-tissue biopsies, etc). Particular aspects provide methods whereby high yields of DNA are isolated as well as a substantial portion of the DNA consists of long DNA fragments, and where the isolated genomic DNA is free of associated or cross-linked contaminants like proteins, peptides, amino acids or RNA. The methods are facile, cost-effective, an are characterized by high reproducibility and reliability. Particular aspects provide methods for providing DNA fragments derived from an archived sample, wherein the yield of DNA before, for example, an amplification step is at least 20%, and amplicons up to a length of about 1,000 base pairs are amplifiable.
摘要翻译：本发明的方面涉及从归档样品（例如石蜡包埋和/或固定组织活组织检查等）提供DNA片段的组合物和方法。具体方面提供了分离DNA的高产率以及大部分DNA由长DNA片段组成并且分离的基因组DNA不含相关或交联的污染物如蛋白质，肽，氨基酸或RNA的方法。这些方法是容易的，具有成本效益的，其特征在于重现性和可靠性高。特定方面提供用于提供衍生自归档样品的DNA片段的方法，其中在例如扩增步骤之前的DNA的产量为至少20％，并且可扩增至约1000个碱基对的扩增子。

9. 发明授权

US08679745B2 Method for providing DNA fragments derived from an archived sample 有权
标题翻译：提供衍生自归档样品的DNA片段的方法
公开(公告)号：US08679745B2
公开(公告)日：2014-03-25
申请号：US11664367
申请日：2005-09-30
申请人： Matthias Ballhause , Kurt Berlin , Dimo Dietrich , Antje Kluth Lukas , Matthias Schuster , Ute Wagner , Reinhold Wasserkort , Heike Ziebarth
发明人： Matthias Ballhause , Kurt Berlin , Dimo Dietrich , Antje Kluth Lukas , Matthias Schuster , Ute Wagner , Reinhold Wasserkort , Heike Ziebarth
IPC分类号： C12Q1/68 , C12P19/34 , B01L3/00
CPC分类号： C12Q1/6806 , C12N15/1003 , C12Q1/6858 , C12Q1/686 , C12Q2523/125 , C12Q2527/137 , C12Q2527/149 , C12Q2531/107
摘要： Aspects of the present invention relate to compositions and methods for providing DNA fragments from an archived sample (e.g., paraffin-embedded and/or fixed-tissue biopsies, etc). Particular aspects provide methods whereby high yields of DNA are isolated as well as a substantial portion of the DNA consists of long DNA fragments, and where the isolated genomic DNA is free of associated or cross-linked contaminants like proteins, peptides, amino acids or RNA. The methods are facile, cost-effective, and are characterized by high reproducibility and reliability. Particular aspects provide methods for providing DNA fragments derived from an archived sample, wherein the yield of DNA before, for example, an amplification step is at least 20%, and amplicons up to a length of about 1,000 base pairs are amplifiable.
摘要翻译：本发明的方面涉及从归档样品（例如石蜡包埋和/或固定组织活组织检查等）提供DNA片段的组合物和方法。具体方面提供了分离DNA的高产率以及大部分DNA由长DNA片段组成并且分离的基因组DNA不含相关或交联的污染物如蛋白质，肽，氨基酸或RNA的方法。该方法简便，成本效益高，重现性和可靠性高。特定方面提供用于提供衍生自归档样品的DNA片段的方法，其中在例如扩增步骤之前的DNA的产量为至少20％，并且可扩增至约1000个碱基对的扩增子。

10. 发明授权

US08900829B2 Methods and nucleic acids for analyses of cellular proliferative disorders 有权
标题翻译：用于分析细胞增殖性疾病的方法和核酸
公开(公告)号：US08900829B2
公开(公告)日：2014-12-02
申请号：US13096932
申请日：2011-04-28
申请人： Juergen Distler , Thomas Hildmann , Ralf Lesche , Catherine Lofton-Day , Fabian Model , Matthias Schuster , Andrew Z. Sledziewski , Reimo Tetzner , Xiaoling Song
发明人： Juergen Distler , Thomas Hildmann , Ralf Lesche , Catherine Lofton-Day , Fabian Model , Matthias Schuster , Andrew Z. Sledziewski , Reimo Tetzner , Xiaoling Song
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6806 , C12Q2600/106 , C12Q2600/112 , C12Q2600/154 , C12Q2600/158
摘要： Aspects of the invention provide methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. Particular aspects disclose and provide genomic sequences the methylation patterns of which have substantial utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.
摘要翻译：本发明的方面提供用于检测或检测和区分肝细胞增殖性疾病或用于检测或检测和鉴别结直肠细胞增殖性疾病之间或之中的方法，核酸和试剂盒。具体方面公开并提供基因组序列，其甲基化模式具有用于改善所述疾病类别的检测和分化的实质性用途，从而能够改善患者的诊断和治疗。

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