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1. 发明申请

US20100120036A1 METHOD FOR AMPLIFYING DNA FRAGMENT 审中-公开
标题翻译：放大DNA片段的方法
公开(公告)号：US20100120036A1
公开(公告)日：2010-05-13
申请号：US12530033
申请日：2008-03-05
申请人： Kunio Shiota , Shintaro Yagi , Yoko Takahashi , Satoshi Tanaka , Jun Ohgane
发明人： Kunio Shiota , Shintaro Yagi , Yoko Takahashi , Satoshi Tanaka , Jun Ohgane
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/683 , C12Q2531/113 , C12Q2525/155 , C12Q2521/331
摘要： To measure the methylation degree of a genomic DNA simply and correctly as well as exhaustively, there is provided a method for amplifying a DNA fragment, characterized by comprising the following steps (1) to (5): (1) digesting a sample double-stranded DNA with a first restriction enzyme; (2) adding a double-stranded DNA fragment consisting of a first oligonucleotide and a second oligonucleotide to a restriction enzyme cleavage end of the double-stranded DNA fragment produced in step (1) to obtain a DNA fragment; (3) digesting the DNA fragment obtained in step (2) with a second restriction enzyme; (4) allowing the double-stranded DNA fragment produced in step (3) to coexist with a third oligonucleotide and a fourth oligonucleotide in the presence of a ligase; and (5) heating the double-stranded DNA fragment obtained in step (4), thereby dissociating the double-stranded DNA fragment and then performing a polymerase chain reaction to selectively amplify a DNA fragment having the cleavage end of the first restriction enzyme and the cleavage end of the second restriction enzyme.
摘要翻译：为了简单正确地，全面地测定基因组DNA的甲基化程度，提供了扩增DNA片段的方法，其特征在于包括以下步骤（1）〜（5）：（1）用第一限制酶的双链DNA; （2）将由第一寡核苷酸和第二寡核苷酸组成的双链DNA片段加入步骤（1）中制备的双链DNA片段的限制酶切割末端以获得DNA片段; （3）用第二限制酶消化步骤（2）中获得的DNA片段; （4）在连接酶存在下使步骤（3）中产生的双链DNA片段与第三寡核苷酸和第四寡核苷酸共存; （5）加热步骤（4）中获得的双链DNA片段，从而解离双链DNA片段，然后进行聚合酶链式反应以选择性扩增具有第一限制酶切割末端的DNA片段，第二限制酶的切割末端。

2. 发明申请

US20060134659A1 Method for demethylating target methylated region of genome DNA 审中-公开
标题翻译：基因组DNA靶甲基化区域去甲基化方法
公开(公告)号：US20060134659A1
公开(公告)日：2006-06-22
申请号：US11221012
申请日：2005-09-06
申请人： Kunio Shiota , Satoshi Tanaka , Jun Ohgane , Takuya Imamura , Naka Hattori , Koichiro Nishino , Soshi Yamamoto , Naoko Hattori
发明人： Kunio Shiota , Satoshi Tanaka , Jun Ohgane , Takuya Imamura , Naka Hattori , Koichiro Nishino , Soshi Yamamoto , Naoko Hattori
IPC分类号： C12Q1/68 , C12N15/87
CPC分类号： C12N15/63 , A01K2217/05 , A61K48/00
摘要： It is an object of the present invention to provide a demethylation method that, from among multiple methylated regions present in the genome DNA of a living cell, can demethylate a specified methylated region only without influencing the other methylated regions, and to achieve the object, a target methylated region present in either a sense or antisense DNA chain in genome DNA of a living cell is demethylated, by artificially generating in the living cell the following (a) RNA comprising a base sequence equivalent to the base sequence of a region comprising all or part of the target methylated region in the DNA chain where the target methylated region is present, or (b) RNA comprising a base sequence equivalent to the base sequence of a region comprising a region neighboring the target methylated region in the DNA chain where the target methylated region is present.
摘要翻译：本发明的目的是提供一种去甲基化方法，其可以在活细胞的基因组DNA中存在的多个甲基化区域中仅对特定的甲基化区域进行去甲基化而不影响其他甲基化区域，为了达到上述目的，存在于活细胞的基因组DNA中的正义或反义DNA链中的目标甲基化区域被去甲基化，通过在活细胞中人造生成以下（a）RNA，其包含与包含全部的区域的碱基序列相当的碱基序列或其中存在目标甲基化区域的DNA链中的部分目标甲基化区域，或（b）包含与DNA链中与靶甲基化区域相邻的区域的碱基序列等同的碱基序列的RNA，其中目标甲基化区存在。

3. 发明授权

US10394311B2 Apparatus and method for information processing 有权
公开(公告)号：US10394311B2
公开(公告)日：2019-08-27
申请号：US15481853
申请日：2017-04-07
申请人： Satoshi Tanaka
发明人： Satoshi Tanaka
IPC分类号： G06F1/324 , G06F1/3293 , G03G15/00 , G06F1/12 , G06F1/3206 , G06F1/3287 , G06F3/12 , G06F9/4401 , G06F9/46 , G06F1/3234 , G06F1/3203
摘要： An information processing apparatus includes a first processor and a second processor. The first processor is operable to perform first processing and second processing in one of a plurality of operating states. The operating states includes a normal state and a power-saving state. The power-saving state is a state in which the first processor consumes less power than the normal state. The second processor is operable to perform the second processing while consuming less power than the first processor does. The second processor detects the first processing that the second processor is not able to perform. The second processor also causes the first processor to transition from the sleep state to the power-saving state to perform the first processing when the first processor is in a sleep state in which a power supply is stopped.

4. 发明授权

US09714408B2 Cell culture method 有权
公开(公告)号：US09714408B2
公开(公告)日：2017-07-25
申请号：US12450480
申请日：2008-04-24
申请人： Satoshi Tanaka , Yoichi Ishizaki , Ryo Suenaga , Yoriko Tokita , Masahito Kogure
发明人： Satoshi Tanaka , Yoichi Ishizaki , Ryo Suenaga , Yoriko Tokita , Masahito Kogure
IPC分类号： C12N5/02 , C12M1/34 , C12M1/00 , C12M1/06 , C12M1/26
CPC分类号： C12M41/32 , C12M23/14 , C12M23/26 , C12M27/02 , C12M29/26 , C12M33/18 , C12M41/26 , C12M41/44
摘要： In a cell culture in a sealed cell culture container, gradual deterioration of culture environment is prevented. A cell culture method in which cell culture is conducted by adding a fresh culture medium to a culture container in which cells and a culture medium are enclosed, which includes the steps ofadjusting the pH of said culture medium to be added to be higher than the pH which is optimum for culture of the cells, and/or adjusting the partial pressure of dissolved carbon dioxide of the culture medium to be added to be lower than the pressure which is optimum for culture of the cells, and/or adjusting the partial pressure of dissolved oxygen of the culture medium to be added to be higher than the pressure which is optimum for culture of the cells; and mixing the adjusted culture medium and the culture medium in said culture-container to adjust at least any of the pH, the partial pressure of dissolved carbon dioxide and the partial pressure of dissolved oxygen to be optimum for culture of said cells, thereby to culture the cells in the culture container.

5. 发明授权

US09603114B2 Communication apparatus 有权
公开(公告)号：US09603114B2
公开(公告)日：2017-03-21
申请号：US14037599
申请日：2013-09-26
申请人： Satoshi Tanaka
发明人： Satoshi Tanaka
IPC分类号： H04W4/00 , H04B5/00 , H04B7/00 , H04W60/00 , H04W48/18 , H04W8/00 , H04W84/12 , H04W88/06 , H04W12/04
CPC分类号： H04W48/18 , H04W4/80 , H04W8/005 , H04W12/04 , H04W48/08 , H04W60/00 , H04W84/12 , H04W88/06
摘要： A first communication apparatus may receive first identification information for identifying a first wireless network and second identification information for identifying a second wireless network via a first type of interface from a second communication apparatus, in a case where the second communication apparatus currently belongs to both the first wireless network and the second wireless network. The first communication apparatus may select, from among the first and second wireless networks, by using the first and the second identification information, at least one wireless network to which the first communication apparatus currently belongs, and execute a wireless communication for target data with the second communication apparatus via a second type of interface by using a target wireless network included in the selected at least one wireless network.

6. 发明授权

US09388376B2 Method for counting subject matters to be counted in container 有权
标题翻译：计算容器中计数的事项的方法
公开(公告)号：US09388376B2
公开(公告)日：2016-07-12
申请号：US14023575
申请日：2013-09-11
申请人： Ryo Suenaga , Yoichi Ishizaki , Satoshi Tanaka , Takahiko Totani , Kyohei Ota
发明人： Ryo Suenaga , Yoichi Ishizaki , Satoshi Tanaka , Takahiko Totani , Kyohei Ota
IPC分类号： C12M1/34 , C12M1/26 , C12M1/00 , C12M1/06
CPC分类号： C12M41/40 , C12M23/14 , C12M23/34 , C12M27/02 , C12M33/18 , C12M41/36
摘要： A method for counting counted targets disposed in a liquid enclosed in a container, includes adjusting a thickness of at least a part of the container; setting at least a part of the adjusted part as a measurement target region; and counting a number of counted targets in the measurement target region.
摘要翻译：一种用于计数设置在容纳在容器中的液体中的计数目标的方法，包括调节容器的至少一部分的厚度; 将所述经调整部分的至少一部分设定为测量对象区域; 并计数测量目标区域中的多个计数目标。

7. 发明授权

US09296305B2 Charging apparatus for vehicles 有权
标题翻译：车辆充电设备
公开(公告)号：US09296305B2
公开(公告)日：2016-03-29
申请号：US13976618
申请日：2011-12-26
申请人： Shinji Ichikawa , Naomi Okamura , Satoshi Tanaka , Atsuhisa Ando , Hiroyuki Okada , Hiroshi Suzuki , Yoshinori Aikawa , Shougo Inamori
发明人： Shinji Ichikawa , Naomi Okamura , Satoshi Tanaka , Atsuhisa Ando , Hiroyuki Okada , Hiroshi Suzuki , Yoshinori Aikawa , Shougo Inamori
IPC分类号： H02J7/00 , H02J7/14 , B60L11/18 , H01M10/44 , H02J7/02
CPC分类号： B60L11/1824 , B60L11/1816 , B60L11/1838 , B60L11/184 , H01M10/44 , H02J5/00 , H02J7/0004 , H02J7/02 , Y02T10/7005 , Y02T10/7072 , Y02T10/92 , Y02T90/121 , Y02T90/128 , Y02T90/14 , Y02T90/163 , Y02T90/169 , Y04S30/14
摘要： Provided is a charging apparatus for vehicles which charges a vehicle provided with a charging control circuit via a charging cable, comprising a charging control device which controls electrical charging to the vehicle by transmitting a charging control signal to the charging control circuit on the vehicle side, a charging control signal transmission restricting device which restricts transmission of the charging control signal, and a resetting device which forcibly resets the charging control signal when said restriction on transmission of the charging control signal is withdrawn.
摘要翻译：提供一种用于通过充电电缆对设置有充电控制电路的车辆充电的车辆的充电装置，包括充电控制装置，其通过向车辆侧的充电控制电路发送充电控制信号来控制对车辆的充电，限制充电控制信号的发送的充电控制信号发送限制装置，以及当所述充电控制信号的发送限制被撤销时强制复位充电控制信号的复位装置。

8. 发明授权

US09224077B2 Control apparatus, image forming apparatus, image forming system, control method, and computer-readable medium 有权
标题翻译：控制装置，图像形成装置，图像形成系统，控制方法以及计算机可读介质
公开(公告)号：US09224077B2
公开(公告)日：2015-12-29
申请号：US13370084
申请日：2012-02-09
申请人： Satoshi Tanaka
发明人： Satoshi Tanaka
IPC分类号： G06K15/02 , H04N1/40 , G06F3/12 , G03G15/08
CPC分类号： G06K15/1868
摘要： A control apparatus includes an operation section, a correction section, and a controller. The operation section performs an operation for forming an image having a predetermined density. The correction section corrects a value of the density. In a case where an image formation condition is switched from a first image formation condition to a second image formation condition, when image formation under the second image formation condition is to be performed on at least a predetermined number of recording media or to be performed for at least a predetermined period, the controller performs control so that the correction section executes a process of correcting the value. When the image formation is not to be performed on at least the predetermined number of recording media or not to be performed for at least the predetermined period, the controller performs control so that the correction section does not execute the process.
摘要翻译：控制装置包括操作部，校正部和控制器。操作部分执行用于形成具有预定浓度的图像的操作。校正部分校正密度值。在将图像形成条件从第一图像形成条件切换到第二图像形成条件的情况下，当要在至少预定数量的记录介质上执行在第二图像形成条件下的图像形成时，或者为至少预定时间段，控制器执行控制，使得校正部分执行校正该值的处理。当至少在至少预定数量的记录介质上执行图像形成或至少在预定时间段内执行图像形成时，控制器执行控制，使得校正部分不执行处理。

9. 发明授权

US09161683B2 Electronic endoscopic apparatus 有权
标题翻译：电子内窥镜装置
公开(公告)号：US09161683B2
公开(公告)日：2015-10-20
申请号：US13527098
申请日：2012-06-19
申请人： Takayuki Sato , Kaoru Kotoda , Hisashi Nishimura , Kazuhiro Takizawa , Motoo Azuma , Satoshi Tanaka , Naruyasu Kobayashi
发明人： Takayuki Sato , Kaoru Kotoda , Hisashi Nishimura , Kazuhiro Takizawa , Motoo Azuma , Satoshi Tanaka , Naruyasu Kobayashi
IPC分类号： H04N7/18 , A61B1/04 , A61B1/00
CPC分类号： A61B1/04 , A61B1/00006 , A61B1/00009 , A61B1/00013 , A61B1/00016 , A61B1/0002 , H04N5/0675 , H04N5/123
摘要： A CMOS sensor converts optical information into an electric signal, and outputs the converted electric signal as an image signal. An imaging clock generating unit generates an imaging clock acting as a source of a drive signal that drives the CMOS sensor. A drive signal generating unit generates an imaging synchronization signal and a drive signal based on the imaging clock. A display clock generating unit generates a display clock. A display synchronization signal generating unit generates a display synchronization signal based on the display clock. A phase comparing unit compares a phase of the imaging synchronization signal with a phase of the display clock, and controls oscillation of the display clock generating unit based on a result of the comparison.
摘要翻译： CMOS传感器将光学信息转换为电信号，并将转换的电信号作为图像信号输出。成像时钟产生单元产生作为驱动CMOS传感器的驱动信号的源的成像时钟。驱动信号生成单元基于成像时钟生成成像同步信号和驱动信号。显示时钟生成单元生成显示时钟。显示同步信号生成单元基于显示时钟生成显示同步信号。相位比较单元将成像同步信号的相位与显示时钟的相位进行比较，并且基于比较的结果来控制显示时钟生成单元的振荡。

10. 发明申请

US20140363339A1 STEAM STERILIZER 有权
标题翻译：蒸汽杀菌剂
公开(公告)号：US20140363339A1
公开(公告)日：2014-12-11
申请号：US14371555
申请日：2012-01-30
申请人： Chihiro Kinebuchi , Eiichi Minemura , Satoshi Tanaka , Haruo Machida , Akihiro Miyamoto , Kouji Tanaka , Hiroshi Karasawa
发明人： Chihiro Kinebuchi , Eiichi Minemura , Satoshi Tanaka , Haruo Machida , Akihiro Miyamoto , Kouji Tanaka , Hiroshi Karasawa
IPC分类号： A61L2/07
CPC分类号： A61L2/07 , F04F5/04 , F04F5/20
摘要： Provided is a steam sterilizer capable of performing water vapor sterilization without using a vacuum pump as a mechanism for bringing the inside of a pressure vessel into vacuum. The steam sterilizer includes a pressure vessel (32) which stores an object to be sterilized and in which sterilization is performed with water vapor supplied from a steam piping, and vacuum-generating means (34) which is connected to the pressure vessel (32) and brings the inside of the pressure vessel (32) into a vacuum state. The vacuum-generating means (34) includes a water ejector (48), a tank (50) for storing supply water supplied from an outside, and a pump (52) for supplying the supply water inside the tank (50) to the water ejector (48).
摘要翻译：提供一种能够不使用真空泵进行水蒸气灭菌的蒸汽灭菌器，作为使压力容器内部成为真空的机构。蒸汽灭菌器包括：压力容器（32），其存储要被灭菌的物体，并且利用从蒸汽管道供应的水蒸气进行灭菌;以及与压力容器（32）连接的真空发生装置（34）并使压力容器（32）的内部成为真空状态。真空发生装置（34）包括水喷射器（48），用于存储从外部供应的供水的罐（50）和用于将水（50）内的供给水供应到水的泵（52）喷射器（48）。

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