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1. 发明授权

US09388376B2 Method for counting subject matters to be counted in container 有权
标题翻译：计算容器中计数的事项的方法
公开(公告)号：US09388376B2
公开(公告)日：2016-07-12
申请号：US14023575
申请日：2013-09-11
申请人： Ryo Suenaga , Yoichi Ishizaki , Satoshi Tanaka , Takahiko Totani , Kyohei Ota
发明人： Ryo Suenaga , Yoichi Ishizaki , Satoshi Tanaka , Takahiko Totani , Kyohei Ota
IPC分类号： C12M1/34 , C12M1/26 , C12M1/00 , C12M1/06
CPC分类号： C12M41/40 , C12M23/14 , C12M23/34 , C12M27/02 , C12M33/18 , C12M41/36
摘要： A method for counting counted targets disposed in a liquid enclosed in a container, includes adjusting a thickness of at least a part of the container; setting at least a part of the adjusted part as a measurement target region; and counting a number of counted targets in the measurement target region.
摘要翻译：一种用于计数设置在容纳在容器中的液体中的计数目标的方法，包括调节容器的至少一部分的厚度; 将所述经调整部分的至少一部分设定为测量对象区域; 并计数测量目标区域中的多个计数目标。

2. 发明申请

US20130164831A1 MULTILAYER FILM AND CELL CULTURE CONTAINER 审中-公开
标题翻译：多层膜和细胞培养容器
公开(公告)号：US20130164831A1
公开(公告)日：2013-06-27
申请号：US13818862
申请日：2011-09-05
申请人： Satoshi Tanaka , Takahiko Totani , Yoichi Ishizaki , Kyohei Ota , Ryo Suenaga , Masahiro Kuninori
发明人： Satoshi Tanaka , Takahiko Totani , Yoichi Ishizaki , Kyohei Ota , Ryo Suenaga , Masahiro Kuninori
IPC分类号： C12M1/04
CPC分类号： C12M23/24 , B32B1/02 , B32B1/08 , B32B7/02 , B32B27/08 , B32B27/32 , B32B2250/02 , B32B2250/03 , B32B2250/242 , B32B2307/31 , B32B2307/412 , B32B2307/72 , B32B2307/724 , B32B2307/732 , B32B2323/046 , B32B2439/40 , C12M23/14 , C12M23/20 , Y10T428/2495 , Y10T428/24992
摘要： Provided are a multilayer film having excellent gas permeability and excellent handling properties and hence is suited for forming a cell culture container, and a cell culture container formed by using the same.A multilayer film used for forming a cell culture container, comprising: a base material composed of a polyethylene-based resin having a density of 0.87 g/cm3 to 0.90 g/cm3; and an inner layer composed of a polyethylene-based resin having a density of 0.896 g/cm3 to 0.93 g/cm3 and forming a cell culture surface. A cell culture container is formed by using this multilayer film.
摘要翻译：提供了具有优异的透气性和优异的处理性能的多层膜，因此适合于形成细胞培养容器和使用该多孔膜形成的细胞培养容器。一种用于形成细胞培养容器的多层膜，包括：由密度为0.87g / cm 3至0.90g / cm 3的聚乙烯类树脂构成的基材; 以及由密度为0.896g / cm 3至0.93g / cm 3的聚乙烯类树脂构成的内层，形成细胞培养面。通过使用该多层膜形成细胞培养容器。

3. 发明申请

US20110318725A1 CELL CULTURE METHOD, CELL CULTURE DEVICE, METHOD FOR COUNTING SUBSJECT MATTERS TO BE COUNTED IN CONTAINER AND DEVICE FOR COUNTING 审中-公开
标题翻译：细胞培养方法，细胞培养装置，用于计数在集装箱中计数的备份物和用于计数的装置的方法
公开(公告)号：US20110318725A1
公开(公告)日：2011-12-29
申请号：US13138589
申请日：2010-03-02
申请人： Ryo Suenaga , Yoichi Ishizaki , Satoshi Tanaka , Takahiko Totani , Kyohei Ota
发明人： Ryo Suenaga , Yoichi Ishizaki , Satoshi Tanaka , Takahiko Totani , Kyohei Ota
IPC分类号： C12N5/09 , C12Q3/00 , C12Q1/02 , C12M3/00 , C12M1/36
CPC分类号： C12M41/40 , C12M23/14 , C12M23/34 , C12M27/02 , C12M33/18 , C12M41/36
摘要： Cell aggregate formation control and cell aggregate disintegration control are carried out with respect to cells in a culture container to increase cell proliferation efficiency. The number of the cells in the culture container is counted without disassembly of a culture system and irrespective of the density of the cells.An external force is applied to the culture container to carry out at least one of cell aggregate formation control and cell aggregate disintegration control with respect to cells in the culture container, thus culturing the cells. The external force is applied to the culture container by pressing an agitating member onto a top surface of the culture container placed in flat orientation to a predetermined pressing degree, and moving the agitating member in a horizontal direction at a predetermined speed, thus controlling at least one of cell aggregate formation and cell aggregate disintegration with respect to the cells in the culture container. A method for counting counted targets disposed in a liquid enclosed in a container includes adjusting a thickness of at least a part of the container. At least a part of the adjusted part is set as a measurement target region. A number of counted targets in the measurement target region is counted.
摘要翻译：对培养容器中的细胞进行细胞聚集体形成控制和细胞聚集体分解控制，以提高细胞增殖效率。在不拆卸培养系统的情况下对培养容器中的细胞数进行计数，而不考虑细胞的密度。对培养容器施加外力，对培养容器中的细胞进行细胞聚集体形成控制和细胞聚集体分解控制中的至少一种，从而培养细胞。通过将搅拌构件按照预定的压制程度将培养容器的上表面压入培养容器的上表面并以预定的速度沿水平方向移动搅拌构件，将外力施加到培养容器上，从而至少控制相对于培养容器中的细胞的细胞聚集体形成和细胞聚集体分解中的一种。用于计数设置在容纳在容器中的液体中的计数目标的方法包括调节至少一部分容器的厚度。调整后的部分的至少一部分被设定为测量对象区域。对测量目标区域中的多个计数目标进行计数。

4. 发明申请

US20100062530A1 CELL CULTURE METHOD, CELL CULTURE SYSTEM AND CULTURE MEDIUM ADJUSTEMENT APPARATUS 有权
标题翻译：细胞培养方法，细胞培养系统和培养中等调节剂
公开(公告)号：US20100062530A1
公开(公告)日：2010-03-11
申请号：US12450480
申请日：2008-04-24
申请人： Satoshi Tanaka , Yoichi Ishizaki , Ryo Suenaga , Yoriko Tokita , Masahito Kogure
发明人： Satoshi Tanaka , Yoichi Ishizaki , Ryo Suenaga , Yoriko Tokita , Masahito Kogure
IPC分类号： C12N5/02 , C12M1/00
CPC分类号： C12M41/32 , C12M23/14 , C12M23/26 , C12M27/02 , C12M29/26 , C12M33/18 , C12M41/26 , C12M41/44
摘要： In a cell culture in a sealed cell culture container, gradual deterioration of culture environment is prevented. A cell culture method in which cell culture is conducted by adding a fresh culture medium to a culture container in which cells and a culture medium are enclosed, which includes the steps ofadjusting the pH of said culture medium to be added to be higher than the pH which is optimum for culture of the cells, and/or adjusting the partial pressure of dissolved carbon dioxide of the culture medium to be added to be lower than the pressure which is optimum for culture of the cells, and/or adjusting the partial pressure of dissolved oxygen of the culture medium to be added to be higher than the pressure which is optimum for culture of the cells; and mixing the adjusted culture medium and the culture medium in said culture container to adjust at least any of the pH, the partial pressure of dissolved carbon dioxide and the partial pressure of dissolved oxygen to be optimum for culture of said cells, thereby to culture the cells in the culture container.
摘要翻译：在密闭的细胞培养容器中的细胞培养物中，防止了培养环境的逐渐恶化。一种细胞培养方法，其中通过向封闭细胞和培养基的培养容器中加入新鲜培养基进行细胞培养，其包括将待加入的培养基的pH调节至高于调节细胞培养最适宜的pH值，和/或将待添加的培养基溶解二氧化碳的分压调整至低于细胞培养最佳的压力，和/或调节分压的待添加培养基的溶解氧高于培养细胞的最佳压力; 并将调节后的培养基和培养液混合，调节pH，溶解二氧化碳的分压和溶解氧的分压中的至少任意一种，使其培养成最适于培养所述细胞，细胞在培养容器中。

5. 发明授权

US09441193B2 Cell culture apparatus, cell culture system and cell culture method 有权
标题翻译：细胞培养装置，细胞培养系统和细胞培养方法
公开(公告)号：US09441193B2
公开(公告)日：2016-09-13
申请号：US12450481
申请日：2008-04-25
申请人： Satoshi Tanaka , Yoichi Ishizaki , Ryo Suenaga , Yoriko Tokita , Masahito Kogure
发明人： Satoshi Tanaka , Yoichi Ishizaki , Ryo Suenaga , Yoriko Tokita , Masahito Kogure
IPC分类号： C12M1/34 , C12M1/00 , C12M1/06 , C12M1/26
CPC分类号： C12M23/34 , C12M23/14 , C12M27/02 , C12M29/00 , C12M33/18
摘要： By maintaining the cell density during culture at an appropriate level and eliminating the procedure of transferring cells from a container to another container when a large amount of cells are cultured, damage on cells can be reduced, risk of contamination can be lowered, labor saving can be attained and automation can be attained.A cell culture apparatus including: a culture container 11 formed of a soft packing material and having an enclosure part 11-1 in which a culture medium and/or cells being cultured are enclosed is placed; a container table 12 on which the culture container 11 is placed; a roller 13 which is placed on the upper surface of the culture container 11 and divides the enclosure part 11-1 into two or more chambers; and a driving means 15 which relatively moves the roller 13 or the culture container 11, thereby to change the volume of the culture part 11-11 in the enclosure part 11-1 in which a culture medium or cells being cultured are enclosed.
摘要翻译：一种细胞培养装置，其特征在于，包括：由软性包装材料形成的培养容器11，具有包封培养基和/或培养细胞的封闭部11-1; 容器台12，其上放置有培养容器11; 辊13，其放置在培养容器11的上表面上并将外壳部分11-1分成两个或更多个腔室; 以及驱动装置15，其使辊13或培养容器11相对移动，从而改变包封培养基或培养细胞的包封部11-1中的培养部11-11的体积。

6. 发明授权

US09714408B2 Cell culture method 有权
公开(公告)号：US09714408B2
公开(公告)日：2017-07-25
申请号：US12450480
申请日：2008-04-24
申请人： Satoshi Tanaka , Yoichi Ishizaki , Ryo Suenaga , Yoriko Tokita , Masahito Kogure
发明人： Satoshi Tanaka , Yoichi Ishizaki , Ryo Suenaga , Yoriko Tokita , Masahito Kogure
IPC分类号： C12N5/02 , C12M1/34 , C12M1/00 , C12M1/06 , C12M1/26
CPC分类号： C12M41/32 , C12M23/14 , C12M23/26 , C12M27/02 , C12M29/26 , C12M33/18 , C12M41/26 , C12M41/44
摘要： In a cell culture in a sealed cell culture container, gradual deterioration of culture environment is prevented. A cell culture method in which cell culture is conducted by adding a fresh culture medium to a culture container in which cells and a culture medium are enclosed, which includes the steps ofadjusting the pH of said culture medium to be added to be higher than the pH which is optimum for culture of the cells, and/or adjusting the partial pressure of dissolved carbon dioxide of the culture medium to be added to be lower than the pressure which is optimum for culture of the cells, and/or adjusting the partial pressure of dissolved oxygen of the culture medium to be added to be higher than the pressure which is optimum for culture of the cells; and mixing the adjusted culture medium and the culture medium in said culture-container to adjust at least any of the pH, the partial pressure of dissolved carbon dioxide and the partial pressure of dissolved oxygen to be optimum for culture of said cells, thereby to culture the cells in the culture container.

7. 发明申请

US20100075406A1 CELL CULTURE APPARATUS, CELL CULTURE SYSTEM AND CELL CULTURE METHOD 有权
标题翻译：细胞培养装置，细胞培养系统和细胞培养方法
公开(公告)号：US20100075406A1
公开(公告)日：2010-03-25
申请号：US12450481
申请日：2008-04-25
申请人： Satoshi Tanaka , Yoichi Ishizaki , Ryo Suenaga , Yoriko Tokita , Masahito Kogure
发明人： Satoshi Tanaka , Yoichi Ishizaki , Ryo Suenaga , Yoriko Tokita , Masahito Kogure
IPC分类号： C12M1/34 , C12M1/00
CPC分类号： C12M23/34 , C12M23/14 , C12M27/02 , C12M29/00 , C12M33/18
摘要： By maintaining the cell density during culture at an appropriate level and eliminating the procedure of transferring cells from a container to another container when a large amount of cells are cultured, damage on cells can be reduced, risk of contamination can be lowered, labor saving can be attained and automation can be attained.A cell culture apparatus including: a culture container 11 formed of a soft packing material and having an enclosure part 11-1 in which a culture medium and/or cells being cultured are enclosed is placed; a container table 12 on which the culture container 11 is placed; a roller 13 which is placed on the upper surface of the culture container 11 and divides the enclosure part 11-1 into two or more chambers; and a driving means 15 which relatively moves the roller 13 or the culture container 11, thereby to change the volume of the culture part 11-11 in the enclosure part 11-1 in which a culture medium or cells being cultured are enclosed.
摘要翻译：通过在培养期间维持细胞密度达到适当的水平，并且消除了当培养大量细胞时将细胞从容器转移到另一容器的过程，可以降低细胞损伤，降低污染的风险，节省劳力实现并实现自动化。一种细胞培养装置，其特征在于，包括：由软性包装材料形成的培养容器11，具有包封培养基和/或培养细胞的封闭部11-1; 容器台12，其上放置有培养容器11; 辊13，其放置在培养容器11的上表面上并将外壳部分11-1分成两个或更多个腔室; 以及驱动装置15，其使辊13或培养容器11相对移动，从而改变包封培养基或培养细胞的包封部11-1中的培养部11-11的体积。

8. 发明申请

US20050196567A1 Heat-resistant resin container and method of producing the same 有权
标题翻译：耐热性树脂容器及其制造方法
公开(公告)号：US20050196567A1
公开(公告)日：2005-09-08
申请号：US11071444
申请日：2005-03-04
申请人： Tsutomu Iwasaki , Masahito Kogure , Hisao Iwamoto , Ryo Suenaga
发明人： Tsutomu Iwasaki , Masahito Kogure , Hisao Iwamoto , Ryo Suenaga
IPC分类号： B32B1/08
CPC分类号： B32B7/12 , B32B27/08 , B32B27/306 , B32B27/325 , B32B27/34 , B32B27/36 , B32B2250/24 , B32B2250/40 , B32B2272/00 , B32B2307/306 , B32B2307/412 , B32B2307/558 , B32B2307/7244 , B32B2307/738 , B32B2439/00 , Y10T428/1352
摘要： A container with a flange having excellent heat resistance and impact resistance in the lower part of the barrel portion and having excellent transparency in the wall despite the container is formed by molding an amorphous polyester sheet, and a method of producing the same.
摘要翻译：具有凸缘的容器在筒部的下部具有优异的耐热性和耐冲击性，并且尽管容器通过模制无定形聚酯片而形成，但是壁的透明性优异，并且其制造方法。

9. 发明授权

US07833467B2 Polyester container having excellent heat resistance and shock resistance and method of producing the same 有权
标题翻译：具有优异的耐热性和抗冲击性的聚酯容器及其制造方法
公开(公告)号：US07833467B2
公开(公告)日：2010-11-16
申请号：US11658508
申请日：2005-07-25
申请人： Ryo Suenaga , Tsutomu Iwasaki , Taku Hosokai , Toshiki Sakaguchi , Hisao Iwamoto
发明人： Ryo Suenaga , Tsutomu Iwasaki , Taku Hosokai , Toshiki Sakaguchi , Hisao Iwamoto
IPC分类号： B29C49/18 , B29C49/64
CPC分类号： B29C49/12 , B29C45/00 , B29C49/06 , B29C49/4823 , B29C51/04 , B29C51/08 , B29C61/02 , B29C2049/1219 , B29C2049/4828 , B29C2049/4838 , B29C2049/4848 , B29C2791/001 , B29C2791/007 , B29K2995/0039 , B29K2995/0041 , B29L2031/7132 , B65D1/16 , Y10S264/906 , Y10T428/1328 , Y10T428/1352
摘要： A polyester container obtained by stretch-forming a preform of a thermoplastic polyester resin formed by injection forming wherein the central portion of the bottom wall is the remaining part of the gate at the time of injection forming, and is formed relatively thicker than the bottom wall surrounding the central portion, and the central portion of the bottom wall is substantially amorphous, and the bottom wall surrounding the central portion is oriented and crystallized. The container exhibits excellent heat resistance and shock resistance despite a thick portion that is the remaining portion of the injection gate is formed at the center of the bottom portion.
摘要翻译：一种聚酯容器，其通过拉伸成形由注射成型形成的热塑性聚酯树脂的预成型体而获得，其中底壁的中心部分是注射成型时的浇口的剩余部分，并且形成为比底壁围绕中心部分，并且底壁的中心部分基本上是无定形的，并且围绕中心部分的底壁被定向和结晶。尽管在底部的中心处形成注入口的剩余部分的厚部分，但是容器表现出优异的耐热性和抗冲击性。

10. 发明授权

US06971370B2 Common rail type fuel injection system 失效
标题翻译：共轨式燃油喷射系统
公开(公告)号：US06971370B2
公开(公告)日：2005-12-06
申请号：US10968080
申请日：2004-10-20
申请人： Ryo Suenaga , Namoru Oki
发明人： Ryo Suenaga , Namoru Oki
IPC分类号： F02M51/00 , F02D41/04 , F02D41/20 , F02D41/38 , F02D41/40 , F02D45/00 , F02M1/00 , F02M37/04
CPC分类号： F02D41/3836 , F02D41/3845 , F02D2250/04
摘要： When a pressure-feeding period of a supply pump and an injection period of an injector overlap and an actual injection quantity is affected by a pump pressure-feeding quantity of fuel supplied by the supply pump, an engine control unit (ECU) calculates the pump pressure-feeding quantity supplied during the injection period and calculates a correction value in accordance with the pump pressure-feeding quantity. The ECU corrects a command injection quantity with the correction value. Thus, even if injection start timing changes in accordance with a change in an operating state and if the pump pressure-feeding quantity supplied during the injection period changes because of the change in the injection start timing, variation in the actual injection quantity can be inhibited. As a result, the injector can inject an optimum quantity of the fuel.
摘要翻译：当供给泵的加压期间和喷射器的喷射期间重叠，实际喷射量受供给泵供给的燃料的泵的供给量的影响时，发动机控制单元（ECU）计算泵在喷射期间供给的加压量，根据泵的供给量计算出修正值。 ECU用修正值校正指令喷射量。因此，即使喷射开始定时根据运转状态的变化而变化，并且由于喷射开始时刻的变化，喷射期间内供给的泵压力进给量发生变化，因此能够抑制实际喷射量的变化。结果，喷射器可以喷射最佳数量的燃料。

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