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1. 发明授权

US08987232B2 Agent for ameliorating brain hypofunction 有权
标题翻译：改善脑功能减退症的药剂
公开(公告)号：US08987232B2
公开(公告)日：2015-03-24
申请号：US13035717
申请日：2011-02-25
申请人： Kunio Shiota , Masayoshi Kuwahara , Takefumi Kikusui , Shintaro Yagi
发明人： Kunio Shiota , Masayoshi Kuwahara , Takefumi Kikusui , Shintaro Yagi
IPC分类号： A61K31/7008 , C08B37/00 , A61P25/00 , A61P25/28
CPC分类号： A61K31/7008
摘要： Provided are effective and highly safe agents, medicaments and the like for ameliorating various disorders caused by brain hypofunction. Also provided is a preventing or ameliorating agent for brain hypofunction containing N-acetyl-D-mannosamine, a pharmaceutical composition for preventing, ameliorating or treating disorders due to brain hypofunction, containing an effective amount of N-acetyl-D-mannosamine and a pharmaceutically acceptable carrier, and a food comprising N-acetyl-D-mannosamine added thereto.
摘要翻译：提供了用于改善由脑功能不全引起的各种疾病的有效且高度安全的药剂，药物等。还提供了含有N-乙酰基-D-甘露糖胺的预防或改善剂，其用于预防，改善或治疗由于脑功能不全引起的疾病的药物组合物，其含有有效量的N-乙酰基-D-甘露糖胺和药学上可接受的载体和包含N-乙酰-D-甘露糖胺的食品。

2. 发明申请

US20100120036A1 METHOD FOR AMPLIFYING DNA FRAGMENT 审中-公开
标题翻译：放大DNA片段的方法
公开(公告)号：US20100120036A1
公开(公告)日：2010-05-13
申请号：US12530033
申请日：2008-03-05
申请人： Kunio Shiota , Shintaro Yagi , Yoko Takahashi , Satoshi Tanaka , Jun Ohgane
发明人： Kunio Shiota , Shintaro Yagi , Yoko Takahashi , Satoshi Tanaka , Jun Ohgane
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/683 , C12Q2531/113 , C12Q2525/155 , C12Q2521/331
摘要： To measure the methylation degree of a genomic DNA simply and correctly as well as exhaustively, there is provided a method for amplifying a DNA fragment, characterized by comprising the following steps (1) to (5): (1) digesting a sample double-stranded DNA with a first restriction enzyme; (2) adding a double-stranded DNA fragment consisting of a first oligonucleotide and a second oligonucleotide to a restriction enzyme cleavage end of the double-stranded DNA fragment produced in step (1) to obtain a DNA fragment; (3) digesting the DNA fragment obtained in step (2) with a second restriction enzyme; (4) allowing the double-stranded DNA fragment produced in step (3) to coexist with a third oligonucleotide and a fourth oligonucleotide in the presence of a ligase; and (5) heating the double-stranded DNA fragment obtained in step (4), thereby dissociating the double-stranded DNA fragment and then performing a polymerase chain reaction to selectively amplify a DNA fragment having the cleavage end of the first restriction enzyme and the cleavage end of the second restriction enzyme.
摘要翻译：为了简单正确地，全面地测定基因组DNA的甲基化程度，提供了扩增DNA片段的方法，其特征在于包括以下步骤（1）〜（5）：（1）用第一限制酶的双链DNA; （2）将由第一寡核苷酸和第二寡核苷酸组成的双链DNA片段加入步骤（1）中制备的双链DNA片段的限制酶切割末端以获得DNA片段; （3）用第二限制酶消化步骤（2）中获得的DNA片段; （4）在连接酶存在下使步骤（3）中产生的双链DNA片段与第三寡核苷酸和第四寡核苷酸共存; （5）加热步骤（4）中获得的双链DNA片段，从而解离双链DNA片段，然后进行聚合酶链式反应以选择性扩增具有第一限制酶切割末端的DNA片段，第二限制酶的切割末端。

3. 发明申请

US20090221066A1 Anti-Methylated Dna Antibody and Method for Production Thereof 审中-公开
标题翻译：抗甲基化Dna抗体及其生产方法
公开(公告)号：US20090221066A1
公开(公告)日：2009-09-03
申请号：US12225675
申请日：2007-03-27
申请人： Kunio Shiota , Shintaro Yagi , Fumiko Sunaga , Keiji Hirabayashi
发明人： Kunio Shiota , Shintaro Yagi , Fumiko Sunaga , Keiji Hirabayashi
IPC分类号： C12N5/22 , C07K16/00
CPC分类号： C07K16/18 , C07K16/44
摘要： The present invention provides a method of preparing an anti-methylated DNA antibody, comprising a step of administering to an animal an oligodeoxyribonucleotide containing a methylated cytosine(s) and an oligodeoxyribonucleotide containing no methylated cytosine. The antibody prepared by this method is capable of detecting a DNA containing a methylated cytosine(s) in a state close to the state of a biological component.
摘要翻译：本发明提供了制备抗甲基化DNA抗体的方法，包括向动物施用含有甲基化胞嘧啶的寡脱氧核糖核苷酸和不含甲基化胞嘧啶的寡脱氧核糖核苷酸的步骤。通过该方法制备的抗体能够检测到接近生物成分状态的甲基化胞嘧啶的DNA。

4. 发明申请

US20060134659A1 Method for demethylating target methylated region of genome DNA 审中-公开
标题翻译：基因组DNA靶甲基化区域去甲基化方法
公开(公告)号：US20060134659A1
公开(公告)日：2006-06-22
申请号：US11221012
申请日：2005-09-06
申请人： Kunio Shiota , Satoshi Tanaka , Jun Ohgane , Takuya Imamura , Naka Hattori , Koichiro Nishino , Soshi Yamamoto , Naoko Hattori
发明人： Kunio Shiota , Satoshi Tanaka , Jun Ohgane , Takuya Imamura , Naka Hattori , Koichiro Nishino , Soshi Yamamoto , Naoko Hattori
IPC分类号： C12Q1/68 , C12N15/87
CPC分类号： C12N15/63 , A01K2217/05 , A61K48/00
摘要： It is an object of the present invention to provide a demethylation method that, from among multiple methylated regions present in the genome DNA of a living cell, can demethylate a specified methylated region only without influencing the other methylated regions, and to achieve the object, a target methylated region present in either a sense or antisense DNA chain in genome DNA of a living cell is demethylated, by artificially generating in the living cell the following (a) RNA comprising a base sequence equivalent to the base sequence of a region comprising all or part of the target methylated region in the DNA chain where the target methylated region is present, or (b) RNA comprising a base sequence equivalent to the base sequence of a region comprising a region neighboring the target methylated region in the DNA chain where the target methylated region is present.
摘要翻译：本发明的目的是提供一种去甲基化方法，其可以在活细胞的基因组DNA中存在的多个甲基化区域中仅对特定的甲基化区域进行去甲基化而不影响其他甲基化区域，为了达到上述目的，存在于活细胞的基因组DNA中的正义或反义DNA链中的目标甲基化区域被去甲基化，通过在活细胞中人造生成以下（a）RNA，其包含与包含全部的区域的碱基序列相当的碱基序列或其中存在目标甲基化区域的DNA链中的部分目标甲基化区域，或（b）包含与DNA链中与靶甲基化区域相邻的区域的碱基序列等同的碱基序列的RNA，其中目标甲基化区存在。

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