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1. 发明申请

US20180045732A1 CORE/SHELL-TYPE FLUORESCENT DYE-CONTAINING NANOPARTICLE AND PRODUCTION METHOD OF THE SAME 审中-公开
公开(公告)号：US20180045732A1
公开(公告)日：2018-02-15
申请号：US15670411
申请日：2017-08-07
申请人： Konica Minolta, Inc.
发明人： Hironori ISHII , Takuji AIMIYA , Kensaku TAKANASHI , Shin NAKAYAMA
IPC分类号： G01N33/58 , C09B67/02 , C08J7/04 , G01N33/543
CPC分类号： G01N33/582 , C08F2/22 , C08F2/44 , C08J7/047 , C08J2325/14 , C08J2461/24 , C08J2461/26 , C08J2461/28 , C09B67/0005 , C09B67/0013 , C09B67/0097 , G01N33/54346 , G01N33/587 , C08F212/08 , C08F220/44 , C08F220/20 , C08F2220/287
摘要： The present invention provides a method of producing core/shell-type fluorescent dye-containing nanoparticles for immunohistochemical staining or live cell imaging, the method including: the step 1 of polymerizing monomers for thermoplastic resin synthesis in the presence of a fluorescent dye and thereby preparing core particles composed of a thermoplastic resin containing the fluorescent dye; and the step 2 of coating the core particles each with a shell layer composed of a thermosetting resin. By the method of producing fluorescent dye-containing nanoparticles according to the present invention, fluorescent dye-containing nanoparticles having a high brightness, whose dye does not elutes into water, physiological saline, culture medium and the like, can be produced, and the fluorescent dye-containing nanoparticles can be effectively utilized in immunohistochemical staining and live cell imaging.

2. 发明申请

US20180024059A1 BIOLOGICAL SUBSTANCE QUANTITATION METHOD, PATHOLOGICAL DIAGNOSIS SUPPORT SYSTEM AND RECORDING MEDIUM STORING COMPUTER READABLE PROGRAM 审中-公开
公开(公告)号：US20180024059A1
公开(公告)日：2018-01-25
申请号：US15549006
申请日：2015-02-10
申请人： Konica Minolta, Inc.
发明人： Hideki GOUDA , Kensaku TAKANASHI , Kohsuke GONDA , Noriaki OHUCHI , Mika WATANABE
IPC分类号： G01N21/64 , G01N33/53 , G06T7/90 , G01N33/50 , G01N33/58 , G06T7/00 , G01N21/93
CPC分类号： G01N21/6428 , G01N21/64 , G01N21/6486 , G01N21/93 , G01N33/48 , G01N33/483 , G01N33/5023 , G01N33/53 , G01N33/582 , G06T7/0016 , G06T7/90 , G06T2207/30024
摘要： A biological substance quantitation method includes the following. A fluorescent image is input, which represents an expression of a specific biological substance in a sample stained with a fluorescent substance by a fluorescent bright spot. A quantitative evaluation value of the fluorescent bright spot is calculated. A standard fluorescent image of a standard sample stained under a same condition as the sample and representing an expression of the biological substance in a standard sample, is input under a same condition as the fluorescent image. A quantitative evaluation value in the standard fluorescent image is calculated under a same condition as the fluorescent image. Based on a correlation between an expression amount of the biological substance in a standard sample measured in advance and the evaluation value in the standard fluorescent image, the evaluation value in the fluorescent image is converted to an expression amount of the biological substance in the sample.

3. 发明申请

US20160018300A1 STAINING AGENT FOR STAINING TISSUE, PRODUCTION METHOD FOR STAINING AGENT FOR STAINING TISSUE AND TISSUE STAINING KIT INCLUDING STAINING AGENT FOR STAINING TISSUE 有权
标题翻译：用于染色组织的染色剂，用于染色组织的染色剂的生产方法和组织染色试剂盒，包括用于染色组织的染色剂
公开(公告)号：US20160018300A1
公开(公告)日：2016-01-21
申请号：US14773148
申请日：2014-03-06
申请人： KONICA MINOLTA, INC.
发明人： Kensaku TAKANASHI , Yasushi NAKANO , Takeshi ISODA , Hideki GOUDA
IPC分类号： G01N1/30 , G01N33/52
CPC分类号： G01N1/30 , G01N33/52 , G01N2001/302
摘要： An object of the present invention is to provide: a staining agent for tissue staining which has an improved fluorescence signal evaluation accuracy; and a tissue staining kit comprising the staining agent. The staining agent for tissue staining contains, as a staining component, dye-resin particles comprising thermosetting resin particles and a fluorescent dye immobilized on the resin particles, wherein the resin particles contains a substituent having an electric charge opposite to that of the fluorescent dye and forms an ionic bond or a covalent bond with the fluorescent dye, and the dye-resin particles have a particle size variation coefficient of 15% or less.
摘要翻译：本发明的目的是提供：具有改善的荧光信号评价精度的组织染色用染色剂; 和包含染色剂的组织染色试剂盒。用于组织染色的染色剂包含作为染色成分的染料树脂颗粒，其包含固定在树脂颗粒上的热固性树脂颗粒和荧光染料，其中树脂颗粒含有具有与荧光染料相反的电荷的取代基，与荧光染料形成离子键或共价键，染料树脂粒子的粒径变化系数为15％以下。

4. 发明申请

US20160011179A1 RESIN PARTICLES FOR FLUORESCENT LABELS 审中-公开
标题翻译：用于荧光标签的树脂颗粒
公开(公告)号：US20160011179A1
公开(公告)日：2016-01-14
申请号：US14771988
申请日：2014-03-04
申请人： KONICA MINOLTA, INC.
发明人： Masaru TAKAHASHI , Kensaku TAKANASHI
IPC分类号： G01N33/533 , G01N33/52 , G01N1/30
CPC分类号： G01N33/533 , C08L101/00 , C09B67/001 , C09B67/0017 , G01N1/30 , G01N21/6428 , G01N33/52 , G01N2001/302 , G01N2021/6441 , G01N2458/00 , G01N2800/7028
摘要： An object of the present invention is to provide a fluorophore having excellent initial emission intensity and light resistance, which is not easily discolored and does not affect the evaluation of the number of bright spots even when it is slightly discolored. The resin particle for fluorescence labeling is characterized by comprising a fluorescent dye immobilized in a resin particle, the fluorescent dye satisfying both of the following conditions (1) and (2): (1) the fluorescent dye alone in an aqueous solution has a concentration-dependent peak emission intensity in a range of 30 to 80 μM; and (2) the emission intensity at a concentration of 100 μM is not less than 80% of the peak emission intensity. It is preferred that the Stoke's shift of the fluorescent dye in an aqueous solution is not less than 25 nm. The fluorescent dye may be subjected to a solubilization treatment (such as an acid treatment). It is preferred that the fluorescent dye be encapsulated in a resin particle comprising a thermosetting resin.
摘要翻译：本发明的目的是提供一种具有优异的初始发光强度和耐光性的荧光团，即使稍微变色，也不容易变色，也不会影响亮点数的评价。用于荧光标记的树脂颗粒的特征在于包含固定在树脂颗粒中的荧光染料，满足以下条件（1）和（2）两者的荧光染料：（1）在水溶液中单独的荧光染料具有浓度相关的峰值发射强度在30至80微米的范围内; （2）浓度为100μM时的发光强度不低于峰值发光强度的80％。优选的是Stoke在水溶液中荧光染料的移动不小于25nm。荧光染料可以进行溶解处理（例如酸处理）。荧光染料优选包封在包含热固性树脂的树脂颗粒中。

5. 发明申请

US20200165666A1 DILUENT FOR FLUORESCENT NANO PARTICLES, KIT FOR IMMUNOFLUORESCENT STAINING WHICH UTILIZES SAME, SOLUTION FOR IMMUNOFLUORESCENT STAINING, IMMUNOFLUORESCENT STAINING METHOD, AND GENE STAINING METHOD 审中-公开
公开(公告)号：US20200165666A1
公开(公告)日：2020-05-28
申请号：US16778698
申请日：2020-01-31
申请人： Konica Minolta, Inc.
发明人： Hideki GOUDA , Kensaku TAKANASHI
IPC分类号： C12Q1/6816 , G01N33/58 , G01N33/574 , G01N21/64 , C12Q1/6886 , G01N33/531 , G01N33/543 , G01N33/533
摘要： Provided is a means for detecting and quantifying a biological substance of interest with an improved accuracy by inhibiting non-specific adsorption of fluorescent nanoparticles and thereby reducing the background noise in immunostaining with fluorescent nanoparticles. Immunostaining is carried out upon diluting fluorescent nanoparticles with a fluorescent nanoparticle diluent which contains 1 to 5% (W/W) of a protein having a molecular weight of 40,000 or higher (e.g., BSA) and 1 to 3% (W/W) of a protein having a molecular weight of less than 40,000 (e.g., casein) and, when casein is used as a low-molecular-weight protein, it is preferred that the β-casein content in the casein is 10% (W/W) or less and the ratio of α-casein and β-casein (α-casein:β-casein) contained in the casein is 40:60 to 60:40 (taking the total amount of α-casein and β-casein as 100).

6. 发明申请

US20180362770A1 PHOSPHOR INTEGRATED DOTS NANOPARTICLES AND LABELING AGENT USING SAME 审中-公开
公开(公告)号：US20180362770A1
公开(公告)日：2018-12-20
申请号：US16061986
申请日：2016-12-06
申请人： Konica Minolta, Inc.
发明人： Takuji AIMIYA , Kensaku TAKANASHI
IPC分类号： C09B67/02 , C09B67/08 , C09B19/00 , C09B29/08 , C09B11/24 , C08J7/12 , C08K5/00 , C08L101/02
摘要： The present invention provides a nanoparticle containing a hydrophobic fluorescent substance accumulated therein and a thermosetting resin as a matrix, which nanoparticle, when used for labeling of a biological substance such as a protein or nucleic acid, has brightness sufficient for allowing pathological diagnosis using a fluorescence image obtained thereby. The present invention is a phosphor integrated dots nanoparticles wherein a thermosetting resin contains a structural unit formed from a raw material containing a hydrophobic substituent, and wherein a fluorescent substance is accumulated in the nanoparticle at least by hydrophobic interaction, preferably further by stacking interaction, with the hydrophobic substituent of the thermosetting resin.

7. 发明申请

US20180128719A1 STAINING AGENT FOR STAINING TISSUE, PRODUCTION METHOD FOR STAINING AGENT FOR STAINING TISSUE AND TISSUE STAINING KIT INCLUDING STAINING AGENT FOR STAINING TISSUE 审中-公开
公开(公告)号：US20180128719A1
公开(公告)日：2018-05-10
申请号：US15865680
申请日：2018-01-09
申请人： Konica Minolta, Inc.
发明人： Kensaku TAKANASHI , Yasushi NAKANO , Takeshi ISODA , Hideki GOUDA
IPC分类号： G01N1/30 , G01N33/52
CPC分类号： G01N1/30 , G01N33/52 , G01N2001/302
摘要： An object of the present invention is to provide: a staining agent for tissue staining which has an improved fluorescence signal evaluation accuracy; and a tissue staining kit comprising the staining agent. The staining agent for tissue staining contains, as a staining component, dye-resin particles comprising thermosetting resin particles and a fluorescent dye immobilized on the resin particles, wherein the resin particles contains a substituent having an electric charge opposite to that of the fluorescent dye and forms an ionic bond or a covalent bond with the fluorescent dye, and the dye-resin particles have a particle size variation coefficient of 15% or less.

8. 发明申请

US20180231466A1 BIOLOGICAL SUBSTANCE DETECTION METHOD 审中-公开
公开(公告)号：US20180231466A1
公开(公告)日：2018-08-16
申请号：US15950648
申请日：2018-04-11
申请人： Konica Minolta, Inc.
发明人： Kensaku TAKANASHI , Hideki GODA , Hisatake OKADA , Yasushi NAKANO , Kohsuke GONDA , Motohiro TAKEDA , Noriaki OHUCHI
IPC分类号： G01N21/64 , G01N33/483 , G01N33/58
CPC分类号： G01N21/6486 , G01N21/6428 , G01N21/6458 , G01N21/6489 , G01N33/4833 , G01N33/582 , G01N33/587 , G01N2021/6439
摘要： A biological substance detection method for detecting a biological substance specifically in a pathological specimen, includes a step of immunologically staining the pathological specimen using a fluorescent label, a step of staining the pathological specimen with a staining reagent for morphology observation purposes (eosin) to observe the morphology of the pathological specimen, a step of irradiating the stained pathological specimen with excited light to cause the emission of a fluorescent and detecting the biological substance in the pathological specimen. In the step of immunologically staining the pathological specimen, a special fluorescent particle for which the excitation wavelength appears in a region that is different from the excitation wavelength region of eosin is used as the fluorescent label.

9. 发明申请

US20170342470A1 DILUENT FOR FLUORESCENT NANO PARTICLES, KIT FOR IMMUNOFLUORESCENT STAINING WHICH UTILIZES SAME, SOLUTION FOR IMMUNOFLUORESCENT STAINING, IMMUNOFLUORESCENT STAINING METHOD, AND GENE STAINING METHOD 审中-公开
公开(公告)号：US20170342470A1
公开(公告)日：2017-11-30
申请号：US15534732
申请日：2015-12-09
申请人： Konica Minolta, Inc.
发明人： Hideki GOUDA , Kensaku TAKANASHI
IPC分类号： C12Q1/68 , G01N21/64 , G01N33/58 , G01N33/574
CPC分类号： C12Q1/6816 , C12Q1/6886 , C12Q2600/158 , G01N21/64 , G01N21/6428 , G01N33/48 , G01N33/53 , G01N33/531 , G01N33/533 , G01N33/536 , G01N33/54346 , G01N33/54393 , G01N33/5748 , G01N33/582 , G01N2021/6439 , G01N2333/71
摘要： [Problem ] Provided is a means for detecting and quantifying a biological substance of interest with an improved accuracy by inhibiting non-specific adsorption of fluorescent nanoparticles and thereby reducing the background noise in immunostaining with fluorescent nanoparticles. [Means for Solution] Immunostaining is carried out upon diluting fluorescent nanoparticles with a fluorescent nanoparticle diluent which contains 1 to 5% (W/W) of a protein having a molecular weight of 40,000 or higher (e,g., BSA) and 1 to 3% (W/W) of a protein having a molecular weight of less than 40,000 (eg ., casein) and, when casein is used as a low-molecular-weight protein, it is preferred that the κ-casein content in the casein is 10% (W/W) or less and the ratio of α-casein and β-casein (α-casein:β-casein) contained in the casein is 40:60 to 60:40 ( taking the total amount of α-casein and β-casein as 100).

10. 发明申请

US20170115300A1 BIOLOGICAL SUBSTANCE DETECTION METHOD 审中-公开
公开(公告)号：US20170115300A1
公开(公告)日：2017-04-27
申请号：US15399489
申请日：2017-01-05
申请人： Konica Minolta, Inc.
发明人： Kensaku TAKANASHI , Hisatake OKADA , Yasushi NAKANO , Kohsuke GONDA , Noriaki OHUCHI , Mika WATANABE
IPC分类号： G01N33/58 , G01N33/574 , G01N21/64 , G01N33/569
CPC分类号： G01N33/582 , G01N21/6428 , G01N33/56966 , G01N33/57492 , G01N2021/6439 , G01N2333/70596
摘要： The present invention provides a biological substance detection method for specifically detecting a biological substance from a pathological specimen, by which method, when immunostaining using a fluorescent label and staining for morphological observation using a staining agent for morphological observation are simultaneously performed, the results of fluorescence observation and immunostaining can be assessed properly even if the fluorescent label and/or the staining agent is/are deteriorated by irradiation with an excitation light. The biological substance detection method according to the present invention is characterized in that the brightness retention rate of an immunostained part is in a range of 80% to 120% in relation to the brightness retention rate of a part stained for morphological observation when the fluorescent label used for the immunostaining is observed.

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