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1. 发明授权

US08101819B2 DGAT genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants 有权
标题翻译： DGAT基因用于增加油籽植物中的种子储存脂质生成和改变的脂肪酸分布
公开(公告)号：US08101819B2
公开(公告)日：2012-01-24
申请号：US12470569
申请日：2009-05-22
申请人： Keith Roesler , Knut Meyer , Howard Glenn Damude , Bo Shen , Changjiang Li , Ericka Bermudez , Mitchell C. Tarczynski
发明人： Keith Roesler , Knut Meyer , Howard Glenn Damude , Bo Shen , Changjiang Li , Ericka Bermudez , Mitchell C. Tarczynski
IPC分类号： A01H1/00 , A01H5/00 , A01H5/10 , A01H15/00 , C12N5/14 , C07H21/04
CPC分类号： C12N15/8247 , A23K10/30 , A23L11/00 , A23V2002/00 , C12N9/1029 , C12Y203/0102
摘要： Transgenic oilseeds having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of null segregant oilseeds are described. Novel DGAT genes are used to achieve the increase in seed storage lipids.
摘要翻译：描述了与无分离油籽油的总脂肪酸含量相比，具有总脂肪酸含量增加至少10％和改变的脂肪酸分布的转基因油籽。新型DGAT基因用于实现种子储存脂质的增加。

2. 发明授权

US08455714B2 DGAT genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants 有权
标题翻译： DGAT基因用于增加油籽植物中的种子储存脂质生成和改变的脂肪酸分布
公开(公告)号：US08455714B2
公开(公告)日：2013-06-04
申请号：US13329939
申请日：2011-12-19
申请人： Keith Roesler , Knut Meyer , Howard Glenn Damude , Bo Shen , Changjiang Li , Ericka Bermudez , Mitchell C. Tarczynski
发明人： Keith Roesler , Knut Meyer , Howard Glenn Damude , Bo Shen , Changjiang Li , Ericka Bermudez , Mitchell C. Tarczynski
IPC分类号： A01H1/00 , A01H5/00 , A01H5/10 , C12N15/82
CPC分类号： C12N15/8247 , A23K10/30 , A23L11/00 , A23V2002/00 , C12N9/1029 , C12Y203/0102
摘要： Transgenic oilseeds having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of null segregant oilseeds are described. Novel DGAT genes are used to achieve the increase in seed storage lipids.
摘要翻译：描述了与无分离油籽油的总脂肪酸含量相比，具有总脂肪酸含量增加至少10％和改变的脂肪酸分布的转基因油籽。新型DGAT基因用于实现种子储存脂质的增加。

3. 发明申请

US20080313770A1 Methods and compositions for modulating tocol content 审中-公开
标题翻译：用于调节肉鸡内容物的方法和组合物
公开(公告)号：US20080313770A1
公开(公告)日：2008-12-18
申请号：US11655654
申请日：2007-01-19
申请人： Knut Meyer , Bo Shen , Mitchell C. Tarczynski
发明人： Knut Meyer , Bo Shen , Mitchell C. Tarczynski
IPC分类号： C12N15/29 , A01H5/00
CPC分类号： C12N15/8247 , A61K9/0024 , A61K9/0043 , C12N15/8243 , C12N15/8271
摘要： Compositions comprising a modulated tocol content in a plant or plant part are provided. In specific embodiments, the compositions and methods of the invention modulate tocol content by modulating the level of a polypeptide having a LEC1-type B domain in combination with modulating the level of at least one other polypeptide involved in tocol biosynthesis. Plants, plant parts, grain, seed and oil having the modulated tocol level are also provided. Methods to enhance oxidative stress tolerance of a plant or plant part, increase shelf-life, enhance the nutritional value, and improve tissue quality are also provided.
摘要翻译：提供了包含植物或植物部分中调制的多克隆内容物的组合物。在具体实施方案中，本发明的组合物和方法通过调节具有LEC1型B结构域的多肽的水平并结合调节参与多巴胺生物合成的至少一种其他多肽的水平来调节多克隆含量。还提供了植物，植物部分，具有调制多酚水平的谷物，种子和油。还提供了增强植物或植物部分的氧化应激耐受性，增加保质期，增强营养价值和改善组织质量的方法。

4. 发明授权

US07981677B2 Identifying a high oil phenotype in maize by detecting a marker locus genetically linked with a QTL6 region 有权
标题翻译：通过检测与QTL6区域遗传连锁的标记位点，识别玉米高油表型
公开(公告)号：US07981677B2
公开(公告)日：2011-07-19
申请号：US11680906
申请日：2007-03-01
申请人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
发明人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
IPC分类号： C12Q1/68 , C07H21/04 , G01N33/92
CPC分类号： A01H1/04 , C12N9/1029 , C12N15/8247 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/172 , Y10T436/104165
摘要： Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci that are genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant or plant part thereof. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods of the invention for manipulating oil and/or oleic acid content and/or oleic acid/lineolic acid ratio of a plant or plant part thereof, for marker-assisted selection of a plant, for example, a maize plant, or plant part thereof, having an increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio, and for marker-assisted breeding of the high oil and/or high oleic acid trait.
摘要翻译：提供了与玉米数量性状基因座6（QTL6）相关的成分及其使用方法。所述组合物是与QTL6遗传连锁的新型分子标记基因座，其与植物或植物部分的油含量增加和/或油酸含量增加和/或油酸/亚油酸含量增加相关。这些新型标记物的特征在于相对于非高油非高油酸玉米植物的QTL6区域的相应标记基因座存在至少一个多态性。在一些实施方案中，新的标记基因座包含玉米DGAT1-2多肽或其生物活性变体的编码序列。本发明的标记基因座及其合适的片段可用于本发明的操作用于标记辅助选择的植物或植物部分的油和/或油酸含量和/或油酸/线性酸比例的方法中具有增加的油含量和/或油酸含量增加和/或油酸/亚油酸比例增加的植物，例如玉米植物或其植物部分，以及高油的标记辅助育种和/或高油酸性状。

5. 发明申请

US20110010795A9 AP2 DOMAIN TRANSCRIPTION FACTOR ODP2 (OVULE DEVELOPMENT PROTEIN 2) AND METHODS OF USE 有权
标题翻译： AP2域转录因子ODP2（OVULE DEVELOPMENT蛋白2）及其使用方法
公开(公告)号：US20110010795A9
公开(公告)日：2011-01-13
申请号：US12503482
申请日：2009-07-15
申请人： William J. Gordon-Kamm , Timothy G. Helentjaris , Keith S. Lowe , Bo Shen , Mitchell C. Tarczynski , Peizhong Zheng
发明人： William J. Gordon-Kamm , Timothy G. Helentjaris , Keith S. Lowe , Bo Shen , Mitchell C. Tarczynski , Peizhong Zheng
IPC分类号： C12N15/82 , C12N15/11 , C12N15/00 , A01H5/00 , C12N5/04
CPC分类号： C12N15/8243 , A01H1/08 , C07K14/415 , C12N15/8201 , C12N15/8205 , C12N15/8209 , C12N15/8223 , C12N15/8231 , C12N15/8233 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8287 , Y02A40/146
摘要： Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译：提供了调节植物发育的方法和组合物。提供编码Ovule Development Protein 2（ODP2）蛋白的核苷酸序列和氨基酸序列。这些序列可以用于各种方法，包括调节发育，发育途径，改变植物中的油含量，提高转化效率，调节胁迫耐受性和调节植物的再生能力。还提供了转化的植物，植物细胞，组织和种子。

6. 发明授权

US07812216B2 Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods of use 有权
标题翻译：与玉米的数量性状基因座6（QTL6）和使用方法有关的成分
公开(公告)号：US07812216B2
公开(公告)日：2010-10-12
申请号：US11680922
申请日：2007-03-01
申请人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
发明人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
IPC分类号： A01H1/00 , A01H5/00 , A01H5/10 , C12N15/82
CPC分类号： C12N15/8247 , C12N9/1029 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/172
摘要： Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci that are genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant or plant part thereof. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods of the invention for manipulating oil and/or oleic acid content and/or oleic acid/lineolic acid ratio of a plant or plant part thereof, for marker-assisted selection of a plant, for example, a maize plant, or plant part thereof, having an increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio, and for marker-assisted breeding of the high oil and/or high oleic acid trait.
摘要翻译：提供了与玉米数量性状基因座6（QTL6）相关的成分及其使用方法。所述组合物是与QTL6遗传连锁的新型分子标记基因座，其与植物或植物部分的油含量增加和/或油酸含量增加和/或油酸/亚油酸含量增加相关。这些新型标记物的特征在于相对于非高油非高油酸玉米植物的QTL6区域的相应标记基因座存在至少一个多态性。在一些实施方案中，新的标记基因座包含玉米DGAT1-2多肽或其生物活性变体的编码序列。本发明的标记基因座及其合适的片段可用于本发明的操作用于标记辅助选择的植物或植物部分的油和/或油酸含量和/或油酸/线性酸比例的方法中具有增加的油含量和/或增加的油酸含量和/或油酸/亚油酸比例增加的植物，例如玉米植物或其植物部分，以及用于高油的标记辅助育种和/或高油酸性状。

7. 发明申请

US20090235391A1 Alteration of Oil Traits in Plants 有权
标题翻译：植物油性状改变
公开(公告)号：US20090235391A1
公开(公告)日：2009-09-17
申请号：US12429498
申请日：2009-04-24
申请人： Stephen M. Allen , William B. Allen , Rebecca E. Cahoon , Sabine Epelbaum , Omolayo O. Famodu , Leslie T. Harvell , Todd J. Jones , Anthony J. Kinney , Theodore M. Klein , Changjiang Li , Igor Cunha Oliveira , Hajime Sakai , Bo Shen , Mitchell C. Tarczynski
发明人： Stephen M. Allen , William B. Allen , Rebecca E. Cahoon , Sabine Epelbaum , Omolayo O. Famodu , Leslie T. Harvell , Todd J. Jones , Anthony J. Kinney , Theodore M. Klein , Changjiang Li , Igor Cunha Oliveira , Hajime Sakai , Bo Shen , Mitchell C. Tarczynski
IPC分类号： C12N15/82 , C12N15/29 , A01H5/00 , A01H5/10 , A01H1/04
CPC分类号： C12N15/8247 , C07K14/415
摘要： The preparation and use of nucleic acid fragments useful in altering the oil phenotype in plants are disclosed. Chimeric constructs incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. Methods for altering the oil phenotype in plants using such nucleic acid fragments also are disclosed.
摘要翻译：公开了可用于改变植物油表型的核酸片段的制备和使用。掺入此类核酸片段和合适调节序列的嵌合构建体可用于产生具有改变的脂质谱的转基因植物。还公开了使用这种核酸片段改变植物油表型的方法。

8. 发明授权

US07579529B2 AP2 domain transcription factor ODP2 (ovule development protein 2) and methods of use 有权
标题翻译： AP2结构域转录因子ODP2（胚珠发育蛋白2）及其使用方法
公开(公告)号：US07579529B2
公开(公告)日：2009-08-25
申请号：US11045802
申请日：2005-01-28
申请人： William J. Gordon-Kamm , Timothy G. Helentjaris , Keith S. Lowe , Bo Shen , Mitchell C. Tarczynski , Peizhong Zheng
发明人： William J. Gordon-Kamm , Timothy G. Helentjaris , Keith S. Lowe , Bo Shen , Mitchell C. Tarczynski , Peizhong Zheng
IPC分类号： C12N15/29 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8243 , A01H1/08 , C07K14/415 , C12N15/8201 , C12N15/8205 , C12N15/8209 , C12N15/8223 , C12N15/8231 , C12N15/8233 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8287 , Y02A40/146
摘要： Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译：提供了调节植物发育的方法和组合物。提供编码Ovule Development Protein 2（ODP2）蛋白的核苷酸序列和氨基酸序列。这些序列可以用于各种方法，包括调节发育，发育途径，改变植物中的油含量，提高转化效率，调节胁迫耐受性和调节植物的再生能力。还提供了转化的植物，植物细胞，组织和种子。

9. 发明授权

US07560623B2 Enzymatic methods for modulating the levels of organic sulfur compounds in plants 有权
标题翻译：用于调节植物中有机硫化合物水平的酶法
公开(公告)号：US07560623B2
公开(公告)日：2009-07-14
申请号：US11365135
申请日：2006-03-01
申请人： Mitchell C. Tarczynski , Changjiang Li , Bo Shen
发明人： Mitchell C. Tarczynski , Changjiang Li , Bo Shen
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8243 , C12N15/8242
摘要： Methods for modulating levels of at least one organic sulfur compound in plants are provided. Also provided are plants and seeds produced by the methods. The methods comprise stably transforming a plant with a DNA construct encoding a cystathionine gamma synthase enzyme, a serine acetyl transferase enzyme and/or other sulfur assimilating enzymes capable of altering the level of at least one organic sulfur compound.
摘要翻译：提供了调节植物中至少一种有机硫化合物水平的方法。还提供了通过这些方法生产的植物和种子。所述方法包括用编码胱硫醚γ-合成酶的DNA构建体，丝氨酸乙酰转移酶和/或能够改变至少一种有机硫化合物的水平的其它硫同化酶稳定转化植物。

10. 发明授权

US07038109B1 Enzymatic methods for modulating the levels of organic sulfur compounds in plants 有权
公开(公告)号：US07038109B1
公开(公告)日：2006-05-02
申请号：US10108739
申请日：2002-03-29
申请人： Mitchell C. Tarczynski , Changjiang Li , Bo Shen
发明人： Mitchell C. Tarczynski , Changjiang Li , Bo Shen
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8243 , C12N15/8242
摘要： Methods for modulating levels of at least one organic sulfur compound in plants are provided. Also provided are plants and seeds produced by the methods. The methods comprise stably transforming a plant with a DNA construct encoding a cystathionine gamma synthase enzyme, a serine acetyl transferase enzyme and/or other sulfur assimilating enzymes capable of altering the level of at least one organic sulfur compound.

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