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1. 发明授权

US5231193A Process for producing ellagic acid 失效
标题翻译：生产鞣花酸的方法
公开(公告)号：US5231193A
公开(公告)日：1993-07-27
申请号：US854799
申请日：1992-03-25
申请人： Kiyoshi Mizusawa , Yasuhiko Imai , Katsumi Yuasa , Hirokazu Koyama , Nobuyuki Yamaji , Shigehiro Kataoka , Tetsuya Oguma
发明人： Kiyoshi Mizusawa , Yasuhiko Imai , Katsumi Yuasa , Hirokazu Koyama , Nobuyuki Yamaji , Shigehiro Kataoka , Tetsuya Oguma
IPC分类号： C07D493/06
CPC分类号： C07D493/06
摘要： A process for producing ellagic acid comprising reacting a tannin having gallic acid residues attached as constituents with an oxidizing agent at pH 7 or higher.
摘要翻译：一种生产鞣花酸的方法，包括使具有作为成分附着的没食子酸残基的单宁与pH7或更高的氧化剂反应。

2. 发明授权

US5110734A Purified cyclodextrinase 失效
标题翻译：纯化CYCLODEXTRINASE
公开(公告)号：US5110734A
公开(公告)日：1992-05-05
申请号：US533856
申请日：1990-06-06
申请人： Tetsuya Oguma , Mamoru Kikuchi , Kiyoshi Mizusawa , Shoichi Tokutake , Nobuyuki Yamaji
发明人： Tetsuya Oguma , Mamoru Kikuchi , Kiyoshi Mizusawa , Shoichi Tokutake , Nobuyuki Yamaji
IPC分类号： C12N9/24 , C12P19/14
CPC分类号： C12Y302/01054 , C12N9/2402 , C12P19/14
摘要： The present invention relates to a novel cyclodextrinase having the specific physicochemical characteristics relating to action, substrate specificities, optimum pH, stable pH, inhibition, activation and molecular, weight. This novel enzyme can be produced by culturing a specified microorganism belonging to the genus Bacillus. The invention also provides a process for preparing maltooligosaccharides by acting the present enzyme to cyclodextrins.
摘要翻译：本发明涉及具有与作用，底物特异性，最佳pH，稳定pH，抑制，活化和分子重量有关的特定物理化学特性的新型环糊精酶。这种新型酶可以通过培养属于芽孢杆菌属的特定微生物来产生。本发明还提供了通过将本发明酶作用于环糊精来制备麦芽低聚糖的方法。

3. 发明授权

US5238825A Preparation and use of a cyclodextrinase for preparing maltooligosaccharides 失效
标题翻译：制备和应用环糊精酶制备麦芽低聚糖
公开(公告)号：US5238825A
公开(公告)日：1993-08-24
申请号：US835592
申请日：1992-02-14
申请人： Tetsuya Oguma , Mamoru Kikuchi , Kiyoshi Mizusawa , Shoichi Tokutake , Nobuyuki Yamaji
发明人： Tetsuya Oguma , Mamoru Kikuchi , Kiyoshi Mizusawa , Shoichi Tokutake , Nobuyuki Yamaji
IPC分类号： C12N9/24 , C12P19/14
CPC分类号： C12Y302/01054 , C12N9/2402 , C12P19/14
摘要： The present invention relates to the preparation of a cylodextrinase having specific physicochemical characteristics relating to action, substrate specificities, optimum pH, stable pH, inhibition, activation and molecular weight by culturing a specified microorganism belonging to the genus Bacillus, and also provides a process for preparing maltooligosaccharides by using the present enzyme on cylodextrins.
摘要翻译：本发明涉及通过培养属于芽孢杆菌属的特定微生物来制备具有与作用，底物特异性，最佳pH，稳定pH，抑制，活化和分子量相关的特定物理化学特性的环糊精酶，并且还提供了通过使用本发明的酶对环糊精制备麦芽低聚糖。

4. 发明授权

US4247632A Methylguanidine-decomposing enzyme and process for its production 失效
标题翻译：甲基胍分解酶及其生产工艺
公开(公告)号：US4247632A
公开(公告)日：1981-01-27
申请号：US37028
申请日：1979-05-08
申请人： Motoo Nakajima , Kiyoshi Mizusawa , Yoshio Shirokane
发明人： Motoo Nakajima , Kiyoshi Mizusawa , Yoshio Shirokane
IPC分类号： C12N9/78 , C12Q1/34 , C12Q1/58
CPC分类号： C12Q1/34 , C12N9/78 , G01N2333/978 , Y10S435/829
摘要： A novel methylguanidine-decomposing enzyme can be obtained by cultivating in a medium a bacterium belonging to Genus Alcaligenes and having an ability to produce a methylguanidine-decomposing enzyme. This methylguanidine-decomposing enzyme has an ability to decompose methylguanidine into methylamine and urea. Its optimum pH range is 10.9-12.3 and its stable pH range is 5.0-10.6.
摘要翻译：通过在属于产碱杆菌属的细菌的培养基中培养并具有产生甲基胍分解酶的能力，可以获得新的甲基胍分解酶。该甲基胍分解酶具有将甲基胍分解成甲胺和尿素的能力。其最适pH范围为10.9-12.3，其稳定pH范围为5.0-10.6。

5. 发明授权

US5665584A DNA fragment containing a tannase gene, a recombinant plasmid, a process for producing tannase, and a promoter 失效
标题翻译：含有鞣酸酶基因的DNA片段，重组质粒，鞣酸酶的制备方法和启动子
公开(公告)号：US5665584A
公开(公告)日：1997-09-09
申请号：US460860
申请日：1995-06-05
申请人： Osamu Hatamoto , Teruo Watarai , Kiyoshi Mizusawa , Eiichi Nakano
发明人： Osamu Hatamoto , Teruo Watarai , Kiyoshi Mizusawa , Eiichi Nakano
IPC分类号： C12N15/09 , C07H21/04 , C12N9/18 , C12N15/00 , C12R1/69
CPC分类号： C12N9/18
摘要： The present invention relates to a DNA fragment of 3,563 base pairs containing a gene coding for tannase and derived from a microorganism belonging to the genus Aspergillus, with the following restriction enzyme map: ##STR1## B: Bam HI, H: Hind III, K: Kpn I, S: Sal I, X: Xba I; a DNA fragment containing a tannase gene coding for the amino acid sequence of (SEQ ID NO:4); a recombinant plasmid comprising the DNA fragment containing the tannase gene inserted into a plasmid vector; a process for producing tannase, comprising culturing a microorganism belonging to the genus Aspergillus capable of producing tannase in medium with the recombinant plasmid, and recovering tannase from the culture; and a promoter represented by the nucleotide sequence of (SEQ ID NO:1). Tannase can be efficiently produced according to the present invention.
摘要翻译：本发明涉及含有编码鞣酸酶的基因并衍生自属于曲霉属的微生物的3,563个碱基对的DNA片段，其具有以下限制酶图：B：Bam HI，H：Hind III，K ：Kpn I，S：Sal I，X：Xba I; 含有编码（SEQ ID NO：4）的氨基酸序列的鞣酸酶基因的DNA片段; 包含插入质粒载体中的含有鞣酸酶基因的DNA片段的重组质粒; 一种鞣酸酶的制造方法，其特征在于，在培养基中培养能够在培养基中生产鞣酸酶的微生物属于属于曲霉属的微生物，从培养物中回收鞣酸酶; 和由（SEQ ID NO：1）的核苷酸序列表示的启动子。根据本发明可以有效地制备鞣酸酶。

6. 发明授权

US4503147A Monomethylamine-oxidizing enzyme 失效
标题翻译：单甲胺氧化酶
公开(公告)号：US4503147A
公开(公告)日：1985-03-05
申请号：US435316
申请日：1982-10-19
申请人： Motoo Nakajima , Kazuo Nakamura , Yoshio Shirokane , Kiyoshi Mizusawa
发明人： Motoo Nakajima , Kazuo Nakamura , Yoshio Shirokane , Kiyoshi Mizusawa
IPC分类号： C12N9/06 , C12Q1/26 , C12R1/05 , C12R1/07
CPC分类号： C12N9/0022 , C12Q1/26 , Y10S435/829 , Y10S435/832
摘要： A monomethylamine-oxidizing enzyme can be obtained by cultivating in a medium a strain which belongs to Genus Bacillus and has an ability to produce a monomethylamine-oxidizing enzyme. This enzyme exhibits several beneficial properties including the ability to oxidatively deaminate the amino group of monomethylamine to produce formaldehyde, ammonia, and hydrogen peroxide. The enzyme exhibits a high substrate specificity for monomethylamine, ethylamine, and n-proplyamine while showing no substrate specificity for benzylamine, dimethylamine, trimethylamine, ethylenediamine and tryamine. In addition, the enzyme is stable through an elevated temperature range permitting faster reaction rates and therefore a shorter overall quantitative evaluation. Another property includes a low Km value which allows smaller quantities of the enzyme to be employed per sample.
摘要翻译：通过在培养基中培养属于芽孢杆菌属的菌株并具有产生单甲胺氧化酶的能力，可以获得单甲胺氧化酶。该酶显示出几种有益的性质，包括使一甲胺的氨基氧化脱氨以产生甲醛，氨和过氧化氢的能力。该酶对单甲胺，乙胺和正丙胺显示出高的底物特异性，同时对苄胺，二甲胺，三甲胺，乙二胺和试胺没有底物特异性。此外，酶在升高的温度范围内是稳定的，允许更快的反应速率，因此较短的总体定量评估。另一种性质包括低Km值，其允许每个样品使用较少量的酶。

7. 发明授权

US4438206A Method for determination of formaldehyde 失效
标题翻译：甲醛测定方法
公开(公告)号：US4438206A
公开(公告)日：1984-03-20
申请号：US441926
申请日：1982-11-15
申请人： Motoo Nakajima , Kiyoshi Mizusawa
发明人： Motoo Nakajima , Kiyoshi Mizusawa
IPC分类号： G01N21/76 , G01N21/78 , G01N31/22 , G01N33/50 , G01N21/64 , G01N21/77
CPC分类号： G01N31/22 , Y10T436/202499
摘要： In a method of formaldehyde determination by measuring the fluorescence of a fluorescent substance formed by allowing a formaldehyde-containing solution to react with a reagent capable of forming said fluorescent substance from formaldehyde, the improvement comprising measuring the fluorescence in the presence of a serum albumin.
摘要翻译：在通过测量通过使含甲醛溶液与能够从甲醛形成所述荧光物质的试剂反应形成的荧光物质的荧光测定的方法中，所述改进包括在血清白蛋白存在下测量荧光。

8. 发明授权

US4061540A Cholesterol oxidaze and process for preparing same 失效
标题翻译：胆固醇氧化物及其制备方法
公开(公告)号：US4061540A
公开(公告)日：1977-12-06
申请号：US700859
申请日：1976-06-29
申请人： Fumihiko Yoshida , Kiyoshi Mizusawa , Kazuo Nakamura
发明人： Fumihiko Yoshida , Kiyoshi Mizusawa , Kazuo Nakamura
IPC分类号： C12N9/04 , C12D13/10
CPC分类号： C12N9/0006 , Y10S435/815 , Y10S435/843
摘要： A novel cholesterol oxidase can be obtained by culturing a strain belonging to the Genus Corynebacterium and having an ability to produce cholesterol oxidase in a medium containing cholesterol.
摘要翻译：可以通过培养属于棒杆菌属的菌株并且具有在含有胆固醇的培养基中产生胆固醇氧化酶的能力来获得新的胆固醇氧化酶。

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