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1. 发明授权

US06569637B1 Diagnostic reagents for renal function disorders and method for analyzing urine samples 失效
标题翻译：肾功能障碍诊断试剂及尿样分析方法
公开(公告)号：US06569637B1
公开(公告)日：2003-05-27
申请号：US09403093
申请日：1999-10-15
申请人： Kazumasa Aoyagi , Motoo Nakajima , Kenkiti Koiso
发明人： Kazumasa Aoyagi , Motoo Nakajima , Kenkiti Koiso
IPC分类号： C12Q166
CPC分类号： C12Q1/34 , C12Q1/66
摘要： The present invention aims at providing a reagent and a method for simply, rapidly, and accurately diagnosing renal dysfunction. According to the present invention, free ATP in a urine sample from a patient suffering from renal dysfunction is determined by a bioluminescent technique using luciferin and luciferase. By determining the value, it is possible to simply, rapidly, and accurately diagnose common renal dysfunction. Moreover, according to the present invention, it is possible to avoid renal dysfunction caused by side-effects of drugs, by collecting daily the urine of a patient who has been administered with carcinostatic that is toxic to kidney such as cisplatin or methotrexate, and analyzing the changes in the ATP level in the urine sample by a bioluminescent technique, thereby detecting hypersensitivity of the patient caused by such drug or by an excessive administration of such drug, or thereby determining an optimal dose (a maximum acceptable dose) of such drug.
摘要翻译：本发明旨在提供一种用于简单，快速和准确地诊断肾功能障碍的试剂和方法。根据本发明，来自患有肾功能障碍的患者的尿样中的游离ATP通过使用荧光素的生物发光技术测定，萤光素酶。通过确定该值，可以简单，快速，准确地诊断常见的肾功能障碍。此外，根据本发明，可以通过每天收集已经施用了对肾脏有毒的癌症的患者的尿液（例如顺铂或甲氨蝶呤）并分析，可以避免由药物的副作用引起的肾功能障碍通过生物发光技术在尿液样品中的ATP水平的变化，从而检测由这种药物引起的患者的超敏反应或通过过量施用该药物，或由此确定这种药物的最佳剂量（最大可接受剂量）。

2. 发明授权

US06238857B1 Method for analyzing intracellular components 有权
标题翻译：分析细胞内成分的方法
公开(公告)号：US06238857B1
公开(公告)日：2001-05-29
申请号：US09555682
申请日：2000-06-02
申请人： Noriaki Hattori , Keiko Yajitate , Motoo Nakajima , Seiji Murakami
发明人： Noriaki Hattori , Keiko Yajitate , Motoo Nakajima , Seiji Murakami
IPC分类号： C12Q100
CPC分类号： G01N33/66 , C12Q1/66 , C12Q2304/60 , G01N2400/16 , G01N2400/18
摘要： A method for analyzing an intracellular component comprising the following steps, and a reagent kit comprising (a) an extraction reagent, (b) branched dextrin or a derivative thereof, and (c) a reagent for analyzing an intracellular component: (1) step of adding an extraction reagent to a sample containing cells to extract the intracellular component; (2) step of adding branched dextrin or a derivative thereof to the sample containing the extraction reagent; and (3) step of analyzing the extracted intracellular component.
摘要翻译：一种分析细胞内成分的方法，包括以下步骤，以及试剂盒，其包含（a）提取试剂，（b）支链糊精或其衍生物，和（c）用于分析细胞内成分的试剂：（1）向含有细胞的样品中添加提取试剂以提取细胞内成分;（2）将含有支链糊精或其衍生物的支链糊精或其衍生物加入到含有提取试剂的样品中的步骤; 和（3）分析提取的细胞内成分的步骤。

3. 发明授权

US4247632A Methylguanidine-decomposing enzyme and process for its production 失效
标题翻译：甲基胍分解酶及其生产工艺
公开(公告)号：US4247632A
公开(公告)日：1981-01-27
申请号：US37028
申请日：1979-05-08
申请人： Motoo Nakajima , Kiyoshi Mizusawa , Yoshio Shirokane
发明人： Motoo Nakajima , Kiyoshi Mizusawa , Yoshio Shirokane
IPC分类号： C12N9/78 , C12Q1/34 , C12Q1/58
CPC分类号： C12Q1/34 , C12N9/78 , G01N2333/978 , Y10S435/829
摘要： A novel methylguanidine-decomposing enzyme can be obtained by cultivating in a medium a bacterium belonging to Genus Alcaligenes and having an ability to produce a methylguanidine-decomposing enzyme. This methylguanidine-decomposing enzyme has an ability to decompose methylguanidine into methylamine and urea. Its optimum pH range is 10.9-12.3 and its stable pH range is 5.0-10.6.
摘要翻译：通过在属于产碱杆菌属的细菌的培养基中培养并具有产生甲基胍分解酶的能力，可以获得新的甲基胍分解酶。该甲基胍分解酶具有将甲基胍分解成甲胺和尿素的能力。其最适pH范围为10.9-12.3，其稳定pH范围为5.0-10.6。

4. 发明授权

US5891659A Bioluminescent adenosine phosphate ester assay and reagent 失效
标题翻译：生物发光磷酸腺苷酯试剂和试剂
公开(公告)号：US5891659A
公开(公告)日：1999-04-06
申请号：US805613
申请日：1997-02-26
申请人： Seiji Murakami , Tatsuya Sakakibara , Naoki Eisaki , Motoo Nakajima , Kazuhiro Imai
发明人： Seiji Murakami , Tatsuya Sakakibara , Naoki Eisaki , Motoo Nakajima , Kazuhiro Imai
IPC分类号： C12Q1/48 , C12Q1/66
CPC分类号： C12Q1/48 , C12Q1/66
摘要： There is provided a bioluminescence reagent comprising at least pyruvate orthophosphate dikinase, phosphoenolpyruvic acid, pyrophosphoric acid, magnesium ion or another metallic ions, luciferin and luciferase, which reagent is such that the amount of luminescence is maintained in a high level and moreover stably without decaying for a long time in a bioluminescence reaction, and there is provided a method for quantitatively determining an adenosine phosphate ester or a substance taking part in the ATP conversion reaction in high sensitivity and high accuracy using an inexpensive and simple measuring apparatus.
摘要翻译：提供了至少包含丙酮酸正磷酸二激酶，磷酸烯醇丙酮酸，焦磷酸，镁离子或其他金属离子，荧光素和荧光素酶的生物发光试剂，其使得发光量保持在高水平，而且稳定而不衰变并且提供了使用便宜且简单的测量装置以高灵敏度和高精度定量测定参与ATP转化反应的磷酸腺苷酯或物质的方法。

5. 发明授权

US06200767B1 ATP eliminator and the process for determining biological cells 有权
标题翻译： ATP消除剂和确定生物细胞的过程
公开(公告)号：US06200767B1
公开(公告)日：2001-03-13
申请号：US09227108
申请日：1999-01-05
申请人： Tatsuya Sakakibara , Seiji Murakami , Noriaki Hattori , Keiko Yajitate , Teruo Watarai , Motoo Nakajima , Kazuhiro Imai
发明人： Tatsuya Sakakibara , Seiji Murakami , Noriaki Hattori , Keiko Yajitate , Teruo Watarai , Motoo Nakajima , Kazuhiro Imai
IPC分类号： C12Q166
CPC分类号： C12Q1/66 , C12Q1/34 , G01N2333/978
摘要： The present invention provides a process for eliminating effectively ATP in a sample, using adenosine phosphate deaminase alone or in combination with at least one enzyme from the group consisting of apyrase, alkaline phosphatase, acid phosphatase, hexokinase and adenosine triphosphatase, a process for determining biological cells contained in foods and beverages in convenient and precise manner in combination with bioluminescence method, and a reagent for the determination thereof.
摘要翻译：本发明提供了一种用于单独使用磷酸腺苷脱氨酶或与至少一种由apyrase，碱性磷酸酶，酸性磷酸酶，己糖激酶和腺苷三磷酸酶组成的组组合的酶来去除样品中的ATP的方法，以生物发光法结合方便，精确地包含在食品和饮料中的细胞，以及用于确定食品和饮料的试剂。

6. 发明授权

US5891702A ATP eliminator and the process for determining biological cells 失效
标题翻译： ATP消除剂和确定生物细胞的过程
公开(公告)号：US5891702A
公开(公告)日：1999-04-06
申请号：US780161
申请日：1996-12-26
申请人： Tatsuya Sakakibara , Seiji Murakami , Noriaki Hattori , Keiko Yajitate , Teruo Watarai , Motoo Nakajima , Kazuhiro Imai
发明人： Tatsuya Sakakibara , Seiji Murakami , Noriaki Hattori , Keiko Yajitate , Teruo Watarai , Motoo Nakajima , Kazuhiro Imai
IPC分类号： C12Q1/06 , C12Q1/34 , C12Q1/66 , G01N21/76 , C12N9/02 , C12N9/14 , C12N9/78 , C12Q1/42
CPC分类号： C12Q1/66 , C12Q1/34 , G01N2333/978
摘要： The present invention provides a process for eliminating effectively ATP in a sample, using adenosine phosphate deaminase alone or in combination with at least one enzyme selected from the group consisting of apyrase, alkaline phosphatase, acid phosphatase, hexokinase and adenosine triphosphatase, a process for determining biological cells contained in foods and beverages in convenient and precise manner in combination with bioluminescence method, and a reagent for the determination thereof.
摘要翻译：本发明提供了一种使用磷酸腺苷脱氨酶单独或与至少一种选自腺苷磷酸酶，碱性磷酸酶，酸性磷酸酶，己糖激酶和腺苷三磷酸酶的酶组合来消除样品中的ATP的方法，确定方法以生物发光法结合方便，精确地包含在食品和饮料中的生物细胞，以及用于确定的试剂。

7. 发明授权

US5246834A Enzyme immunoassay method employing acetate kinase 失效
标题翻译：使用乙酸激酶的酶免疫方法
公开(公告)号：US5246834A
公开(公告)日：1993-09-21
申请号：US836859
申请日：1992-02-19
申请人： Akio Tsuji , Masako Maeda , Motoo Nakajima
发明人： Akio Tsuji , Masako Maeda , Motoo Nakajima
IPC分类号： G01N33/532 , C12Q1/66 , G01N33/535 , G01N33/543 , G01N33/58
CPC分类号： C12Q1/66 , G01N33/581
摘要： The present invention is intended to provide an EIA method utilizing bioluminescence using a firefly luciferase. The EIA method of the present invention is characterized by using an ATP-generating enzyme as a labeling enzyme, subjecting ATP generated to bioluminescence by the use of the firefly luciferase, and measuring the quantity of light emitted. Acetate kinase is a preferred enzyme used in the method.

8. 发明授权

US4769145A Centrifugal ultrafilter unit for ultrafiltration of biochemical solutions 失效
标题翻译：用于生化溶液超滤的离心超滤单元
公开(公告)号：US4769145A
公开(公告)日：1988-09-06
申请号：US713657
申请日：1985-03-19
申请人： Motoo Nakajima
发明人： Motoo Nakajima
IPC分类号： G01N33/48 , B01D61/18 , B01D63/08 , B04B5/02 , B01D13/00
CPC分类号： B01D61/18 , B01L3/5021 , B01L3/5635 , B01L2300/042 , B01L2300/0681 , B01L2400/0409
摘要： A separator (1; 51) including a solution reservoir (3; 53) having a solution chamber (3a; 53a) for putting therein a solution, a membrane support base (4; 54) joined to the solution reservoir, an ultrafiltration-oriented filtering membrane (5; 55) held between the solution reservoir and the support base, and a filtrate cup (2) attached to the support base, in which the solution reservoir and the support base are each respectively made of a thermoplastic material, wherein the solution reservoir is ultrasonically welded to the support base at a part (3b; 53b) thereof spaced apart by a predetermined distance (l.sub.1 ; l.sub.2) from the filtering membrane.
摘要翻译： 1.一种分离器（1; 51），包括：溶液储存器（3; 53），其具有用于放入溶液的溶液室（3a; 53a），连接到溶液储存器的膜支撑基底（4; 54），超滤取向保持在溶液储存器和支撑基座之间的过滤膜（5; 55）和附着到支撑基座的滤杯（2），其中溶液储存器和支撑基座分别由热塑性材料制成，其中溶液储存器在与过滤膜间隔开预定距离（l1; l2）的部分（3b; 53b）处超声波焊接到支撑基部。

9. 发明授权

US4552849A Method for determination of formaldehyde 失效
标题翻译：甲醛测定方法
公开(公告)号：US4552849A
公开(公告)日：1985-11-12
申请号：US632000
申请日：1984-07-18
申请人： Motoo Nakajima
发明人： Motoo Nakajima
IPC分类号： G01N21/78 , G01N21/64 , G01N21/76 , G01N31/00 , G01N31/22 , G01N21/77
CPC分类号： G01N21/643 , G01N21/6428 , Y10T436/202499
摘要： In a method of formaldehyde determination by measuring the fluorescence of a fluorescent substance formed by the reaction of a formaldehyde-containing solution with a fluorescent reagent capable of forming said fluorescent substance by the reaction with formaldehyde, the improvement which comprises using as said fluorescence reagent a compound represented by the general formula CH.sub.3 C(NH.sub.2).dbd.CHCO.sub.2 R, wherein R represents an alkyl group having 1 to 4 carbon atoms.
摘要翻译：在通过测量由含甲醛溶液与能够通过与甲醛反应形成所述荧光物质的荧光试剂的反应形成的荧光物质的荧光的甲醛测定方法中，包括使用作为所述荧光试剂a 由通式CH 3 C（NH 2）= CHCO 2 R表示的化合物，其中R表示具有1至4个碳原子的烷基。

10. 发明授权

US4503147A Monomethylamine-oxidizing enzyme 失效
标题翻译：单甲胺氧化酶
公开(公告)号：US4503147A
公开(公告)日：1985-03-05
申请号：US435316
申请日：1982-10-19
申请人： Motoo Nakajima , Kazuo Nakamura , Yoshio Shirokane , Kiyoshi Mizusawa
发明人： Motoo Nakajima , Kazuo Nakamura , Yoshio Shirokane , Kiyoshi Mizusawa
IPC分类号： C12N9/06 , C12Q1/26 , C12R1/05 , C12R1/07
CPC分类号： C12N9/0022 , C12Q1/26 , Y10S435/829 , Y10S435/832
摘要： A monomethylamine-oxidizing enzyme can be obtained by cultivating in a medium a strain which belongs to Genus Bacillus and has an ability to produce a monomethylamine-oxidizing enzyme. This enzyme exhibits several beneficial properties including the ability to oxidatively deaminate the amino group of monomethylamine to produce formaldehyde, ammonia, and hydrogen peroxide. The enzyme exhibits a high substrate specificity for monomethylamine, ethylamine, and n-proplyamine while showing no substrate specificity for benzylamine, dimethylamine, trimethylamine, ethylenediamine and tryamine. In addition, the enzyme is stable through an elevated temperature range permitting faster reaction rates and therefore a shorter overall quantitative evaluation. Another property includes a low Km value which allows smaller quantities of the enzyme to be employed per sample.
摘要翻译：通过在培养基中培养属于芽孢杆菌属的菌株并具有产生单甲胺氧化酶的能力，可以获得单甲胺氧化酶。该酶显示出几种有益的性质，包括使一甲胺的氨基氧化脱氨以产生甲醛，氨和过氧化氢的能力。该酶对单甲胺，乙胺和正丙胺显示出高的底物特异性，同时对苄胺，二甲胺，三甲胺，乙二胺和试胺没有底物特异性。此外，酶在升高的温度范围内是稳定的，允许更快的反应速率，因此较短的总体定量评估。另一种性质包括低Km值，其允许每个样品使用较少量的酶。

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