专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US08071351B2 Family 44 xyloglucanases 有权
标题翻译：家族44木葡聚糖酶
公开(公告)号：US08071351B2
公开(公告)日：2011-12-06
申请号：US12779112
申请日：2010-05-13
申请人： Kirk Schnorr , Martin Schulein , Hanne Dela, legal representative , Per Lina Jorgensen
发明人： Kirk Schnorr , Martin Schulein , Per Lina Jorgensen
IPC分类号： C12N9/42 , C12N15/00 , C12N1/20 , C11D3/386 , C12Q1/34 , C12P21/04 , C07H21/04
CPC分类号： C12N9/24 , C11D3/38636 , C11D3/38645 , C12N9/2405 , C12N9/248 , C12Y302/01151 , D06L4/40 , D06M16/003
摘要： The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus Paenibacillus, especially from a strain of Paenibacillus polymyxa or Paenibacillus sp. The xyloglucanases exhibit high performance in conventional detergent compositions.
摘要翻译：本发明涉及属于糖基水解酶家族44的木葡聚糖酶，并且具有pH5至pH8之间至少30％的相对木葡聚糖酶活性，来源于Paenibacillus属，特别是来自多粘杆菌（Paenibacillus polymyxa）或Paenibacillus sp。木葡聚糖酶在常规洗涤剂组合物中表现出高性能。

2. 发明授权

US07361736B2 Family 44 xyloglucanases 有权
标题翻译：家族44木葡聚糖酶
公开(公告)号：US07361736B2
公开(公告)日：2008-04-22
申请号：US10896555
申请日：2004-07-22
申请人： Kirk Schnorr , Per Lina Jorgensen , Hanne Dela, legal representative
发明人： Martin Schulein
IPC分类号： C07K14/00 , C07H21/04 , A61K38/00
CPC分类号： C12N9/24 , C11D3/38636 , C11D3/38645 , C12N9/2405 , C12N9/248 , C12Y302/01151 , D06L4/40 , D06M16/003
摘要： The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus Paenibacillus especially from a strain of Paenibacillus polymyxa or Paenibacillus sp. The xyloglucanases exhibit high performance in conventional detergent compositions.
摘要翻译：本发明涉及属于糖基水解酶家族44的木葡聚糖酶，并且具有pH5至pH8之间至少30％的相对木葡聚糖酶活性，来源于Paenibacillus属，特别是源于多粘杆菌（Paenibacillus polymyxa）或Paenibacillus sp。木葡聚糖酶在常规洗涤剂组合物中表现出高性能。

3. 发明申请

US20080138880A1 FAMILY 44 XYLOGLUCANASES 审中-公开
标题翻译：家庭44 XYLOGLUCANASES
公开(公告)号：US20080138880A1
公开(公告)日：2008-06-12
申请号：US11970559
申请日：2008-01-08
申请人： Kirk Schnorr , Per Lina Jorgensen , Martin Schulein , Hanne Dela
发明人： Kirk Schnorr , Per Lina Jorgensen , Martin Schulein , Hanne Dela
IPC分类号： C12N9/24 , D06M16/00
CPC分类号： C12N9/24 , C11D3/38636 , C11D3/38645 , C12N9/2405 , C12N9/248 , C12Y302/01151 , D06L4/40 , D06M16/003
摘要： The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus Paenibacillus, especially from a strain of Paenibacillus polymyxa or Paenibacillus sp. The xyloglucanases exhibit high performance in conventional detergent compositions.
摘要翻译：本发明涉及属于糖基水解酶家族44的木葡聚糖酶，并且具有pH5至pH8之间至少30％的相对木葡聚糖酶活性，来源于Paenibacillus属，特别是来自多粘杆菌（Paenibacillus polymyxa）或Paenibacillus sp。木葡聚糖酶在常规洗涤剂组合物中表现出高性能。

4. 发明授权

US08354263B2 Family 44 xyloglucanases 有权
公开(公告)号：US08354263B2
公开(公告)日：2013-01-15
申请号：US13287166
申请日：2011-11-02
申请人： Kirk Schnorr , Per Lina Jorgensen , Hanne Dela
发明人： Kirk Schnorr , Martin Schulein , Per Lina Jorgensen
IPC分类号： C12N9/42 , C12N15/00 , C12N1/20 , C11D3/386 , C12Q1/34 , C12P21/04 , C07H21/04
CPC分类号： C12N9/24 , C11D3/38636 , C11D3/38645 , C12N9/2405 , C12N9/248 , C12Y302/01151 , D06L4/40 , D06M16/003
摘要： The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus Paenibacillus, especially from a strain of Paenibacillus polymyxa or Paenibacillus sp. The xyloglucanases exhibit high performance in conventional detergent compositions.

5. 发明申请

US20120079665A1 FAMILY 44 XYLOGLUCANASES 有权
标题翻译：家庭44 XYLOGLUCANASES
公开(公告)号：US20120079665A1
公开(公告)日：2012-04-05
申请号：US13287166
申请日：2011-11-02
申请人： Kirk Schnorr , Per Lina Jorgensen , Martin Schulein , Hanne Dela
发明人： Kirk Schnorr , Per Lina Jorgensen , Martin Schulein , Hanne Dela
IPC分类号： C12N9/24 , C11D3/386 , D06L1/20 , C12S11/00
CPC分类号： C12N9/24 , C11D3/38636 , C11D3/38645 , C12N9/2405 , C12N9/248 , C12Y302/01151 , D06L4/40 , D06M16/003
摘要： The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus Paenibacillus, especially from a strain of Paenibacillus polymyxa or Paenibacillus sp. The xyloglucanases exhibit high performance in conventional detergent compositions.
摘要翻译：本发明涉及属于糖基水解酶家族44的木葡聚糖酶，并且具有pH5至pH8之间至少30％的相对木葡聚糖酶活性，来源于Paenibacillus属，特别是来自多粘杆菌（Paenibacillus polymyxa）或Paenibacillus sp。木葡聚糖酶在常规洗涤剂组合物中表现出高的性能。

6. 发明申请

US20100212091A1 FAMILY 44 XYLOGLUCANASES 有权
标题翻译：家庭44 XYLOGLUCANASES
公开(公告)号：US20100212091A1
公开(公告)日：2010-08-26
申请号：US12779112
申请日：2010-05-13
申请人： Kirk Schnorr , Per Lina Jorgensen , Martin Schulein , Hanne Dela
发明人： Kirk Schnorr , Per Lina Jorgensen , Martin Schulein , Hanne Dela
IPC分类号： C11D3/386 , C12N9/24
CPC分类号： C12N9/24 , C11D3/38636 , C11D3/38645 , C12N9/2405 , C12N9/248 , C12Y302/01151 , D06L4/40 , D06M16/003
摘要： The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus Paenibacillus, especially from a strain of Paenibacillus polymyxa or Paenibacillus sp. The xyloglucanases exhibit high performance in conventional detergent compositions.
摘要翻译：本发明涉及属于糖基水解酶家族44的木葡聚糖酶，并且具有pH5至pH8之间至少30％的相对木葡聚糖酶活性，来源于Paenibacillus属，特别是来自多粘杆菌（Paenibacillus polymyxa）或Paenibacillus sp。木葡聚糖酶在常规洗涤剂组合物中表现出高性能。

7. 发明授权

US6060274A Extracellular expression of cellulose binding domains (CBD) using Bacillus 失效
标题翻译：使用芽孢杆菌的纤维素结合结构域（CBD）的细胞外表达
公开(公告)号：US6060274A
公开(公告)日：2000-05-09
申请号：US959212
申请日：1997-10-28
申请人： Mads Eskelund Bjornvad , Martin Schulein , Per Lina Jorgensen
发明人： Mads Eskelund Bjornvad , Martin Schulein , Per Lina Jorgensen
IPC分类号： C12N15/09 , C07K14/195 , C07K14/21 , C07K14/33 , C12N1/21 , C12P21/02 , C12Q1/68 , C12R1/07 , C12P21/06 , C12N1/20 , C12N15/00
CPC分类号： C07K14/195 , C07K14/21 , C07K14/33
摘要： The present invention relates to Bacillus hosts transformed with a vector comprising a DNA sequence encoding for a cellulose binding domain (CBD) and capable of expressing said sequence, the expressed polypeptide protein consisting essentially of one or more non-catalytic domains; the cellulose binding domain having a molecular weight in the range of from 4 kD to 35 kD and being obtainable from a microorganism or from a plant, preferably from a bacterium or a fungus; the Bacillus host e.g. being one of the species Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus stearothermophilos, and Bacillus amyloliquefaciens; and a Bacillus expression vector carrying an inserted DNA sequence encoding for a cellulose binding domain; and a method for producing a cellulose binding domain polypeptide in a Bacillus host cell.
摘要翻译：本发明涉及用包含编码纤维素结合结构域（CBD）并能够表达所述序列的DNA序列的载体转化的芽孢杆菌宿主，所述表达的多肽蛋白质基本上由一个或多个非催化结构域组成; 所述纤维素结合结构域具有4kD至35kD的分子量，可从微生物或植物，优选来自细菌或真菌得到; 芽孢杆菌宿主例如是枯草芽孢杆菌，地衣芽孢杆菌，巨大芽孢杆菌，嗜热脂肪芽孢杆菌和解淀粉芽孢杆菌之一; 和携带编码纤维素结合结构域的插入DNA序列的芽孢杆菌表达载体; 以及在芽孢杆菌宿主细胞中生产纤维素结合结构域多肽的方法。

8. 发明授权

US06268197B1 Xyloglucan-specific alkaline xyloglucanase from bacillus 失效
标题翻译：来自芽孢杆菌的葡聚糖特异性碱性木葡聚糖酶
公开(公告)号：US06268197B1
公开(公告)日：2001-07-31
申请号：US09110959
申请日：1998-07-07
申请人： Martin Schulein , Helle Outtrup , Per Lina Jorgensen , Mads Eskelund Bjornvad
发明人： Martin Schulein , Helle Outtrup , Per Lina Jorgensen , Mads Eskelund Bjornvad
IPC分类号： C12N942
CPC分类号： C12N9/2434 , C11D3/38636
摘要： A xyloglucanase having a relative xyloglucanase activity of at least 50% at pH 7 and either no or an insignificant cellulolytic activity is obtainable e.g. from a strain of Bacillus. A xyloglucanase comprising an amino acid sequence as shown in positions 30-261 of SEQ ID NO:2 or homologues may be derived from eg Bacillus licheniformis, ATCC 14580, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 88 to nucleotide 783; and a xyloglucanase comprising an amino acid sequence as shown in positions 1-537 of SEQ ID NO:4 or homologues may be derived from eg B. agaradhaerens, NCIMB 40482, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:3 from nucleotide 1 to nucleotide 1611. The xyloglucanases are useful e.g. in cleaning compositions and for treatment of cellulosic fibres.
摘要翻译：在pH7下具有至少50％的相对木葡聚糖酶活性和无或不显着的纤维素分解活性的木葡聚糖酶可以例如获得。来自芽孢杆菌菌株。包含SEQ ID NO：2的位置30-261所示的氨基酸序列或同源物的木葡聚糖酶可以衍生自例如地衣芽孢杆菌ATCC 14580，并且可以由包含如SEQ ID NO所示的核苷酸序列的多核苷酸分子编码：1个从核苷酸88到核苷酸783; 并且包含SEQ ID NO：4的位置1-537所示的氨基酸序列或同源物的木葡聚糖酶可以衍生自例如琼脂糖琼脂糖凝胶，NCIMB 40482，并且可以由包含SEQ ID NO：4所示的核苷酸序列的多核苷酸分子编码 SEQ ID NO：3从核苷酸1到核苷酸1611.木葡聚糖酶是有用的，例如在清洁组合物和纤维素纤维的处理中。

9. 发明申请

US20050009166A1 Cellulase variants 审中-公开
标题翻译：纤维素酶变体
公开(公告)号：US20050009166A1
公开(公告)日：2005-01-13
申请号：US10919195
申请日：2004-08-16
申请人： Kim Andersen , Martin Schulein , Hanne Dela , Lars Christiansen , Bo Damgaard , Claus Von der Osten
发明人： Kim Andersen , Martin Schulein , Hanne Dela , Lars Christiansen , Bo Damgaard , Claus Von der Osten
IPC分类号： C12N15/09 , C11D3/386 , C12N1/15 , C12N1/21 , C12N9/42 , C12R1/645 , C12S11/00 , C12S99/00 , D06L4/40 , D06M16/00 , D21B1/02 , D21C5/02 , D21H21/24 , C07H21/04 , C12N15/74
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.
摘要翻译：本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。鉴定酶的表面暴露的氨基酸残基; e。鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。通过使用常规蛋白质工程技术进行取代，缺失或插入。还描述了通过该方法获得的纤维素酶变体。

10. 发明申请

US20050250181A1 Cell-wall degrading enzyme variants 审中-公开
标题翻译：细胞壁降解酶变体
公开(公告)号：US20050250181A1
公开(公告)日：2005-11-10
申请号：US10926709
申请日：2004-08-26
申请人： Sanne Schroder Glad , Carsten Andersen , Martin Schulein , Hanne Dela , Torben Frandsen
发明人： Sanne Schroder Glad , Carsten Andersen , Martin Schulein , Hanne Dela , Torben Frandsen
IPC分类号： C07H21/04 , C11D3/386 , C11D7/42 , C12N1/21 , C12N9/18 , C12N9/88 , C12N15/74
CPC分类号： C11D3/38636 , C12N9/88
摘要： The present invention relates to variants of a cell-wall degrading enzyme having a beta-helix structure, which variant has at least one substituent in a position determined by identifying all residues potentially belonging to a stack; characterizing the stack as interior or exterior; characterizing the stack as polar, hydrophobic or aromatic/heteroaromatic based on the dominating characteristics of the parent or wild-type enzyme stack residues and/or its orientation relative to the beta-helix (interior or exterior); optimizing all stack positions of a stack either to hydrophobic aliphatic amino acids, hydrophobic aromatic or polar amino acids by allowing mutations within one or all positions to amino acids belonging to one of these groups; measuring thermostability of the variants by DSC or an application-related assay such as a Pad-Steam application test; and selecting the stabilized variants. Variants of a wild-type parent pectate lyase (EC 4.2.2.2) having the conserved amino acid residues D111, D141 or E141, D145, K165, R194 and R199 when aligned with the pectate lyase comprising the amino acid sequence of SEQ ID NO: 2 are preferred.
摘要翻译：本发明涉及具有β-螺旋结构的细胞壁降解酶的变体，该变体具有至少一个取代基，其位置通过鉴定潜在属于堆叠的所有残基而确定; 将堆栈表征为内部或外部; 基于母体或野生型酶堆残基的主导特征和/或其相对于β-螺旋（内部或外部）的取向，将所述叠层表征为极性，疏水性或芳族/杂芳族化合物; 通过在属于这些基团之一的氨基酸的一个或所有位置内允许突变将堆叠的所有堆叠位置优化为疏水性脂族氨基酸，疏水性芳族或极性氨基酸; 通过DSC测量变体的热稳定性或应用相关测定，例如Pad-Steam应用测试; 并选择稳定的变体。当与包含SEQ ID NO：1的氨基酸序列的果胶酸裂合酶比对时，具有保守氨基酸残基D111，D141或E141，D145，K165，R194和R199的野生型亲本果胶酸裂解酶（EC 4.2.2.2） 2是优选的。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式