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1. 发明授权

US06268197B1 Xyloglucan-specific alkaline xyloglucanase from bacillus 失效
标题翻译：来自芽孢杆菌的葡聚糖特异性碱性木葡聚糖酶
公开(公告)号：US06268197B1
公开(公告)日：2001-07-31
申请号：US09110959
申请日：1998-07-07
申请人： Martin Schulein , Helle Outtrup , Per Lina Jorgensen , Mads Eskelund Bjornvad
发明人： Martin Schulein , Helle Outtrup , Per Lina Jorgensen , Mads Eskelund Bjornvad
IPC分类号： C12N942
CPC分类号： C12N9/2434 , C11D3/38636
摘要： A xyloglucanase having a relative xyloglucanase activity of at least 50% at pH 7 and either no or an insignificant cellulolytic activity is obtainable e.g. from a strain of Bacillus. A xyloglucanase comprising an amino acid sequence as shown in positions 30-261 of SEQ ID NO:2 or homologues may be derived from eg Bacillus licheniformis, ATCC 14580, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 88 to nucleotide 783; and a xyloglucanase comprising an amino acid sequence as shown in positions 1-537 of SEQ ID NO:4 or homologues may be derived from eg B. agaradhaerens, NCIMB 40482, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:3 from nucleotide 1 to nucleotide 1611. The xyloglucanases are useful e.g. in cleaning compositions and for treatment of cellulosic fibres.
摘要翻译：在pH7下具有至少50％的相对木葡聚糖酶活性和无或不显着的纤维素分解活性的木葡聚糖酶可以例如获得。来自芽孢杆菌菌株。包含SEQ ID NO：2的位置30-261所示的氨基酸序列或同源物的木葡聚糖酶可以衍生自例如地衣芽孢杆菌ATCC 14580，并且可以由包含如SEQ ID NO所示的核苷酸序列的多核苷酸分子编码：1个从核苷酸88到核苷酸783; 并且包含SEQ ID NO：4的位置1-537所示的氨基酸序列或同源物的木葡聚糖酶可以衍生自例如琼脂糖琼脂糖凝胶，NCIMB 40482，并且可以由包含SEQ ID NO：4所示的核苷酸序列的多核苷酸分子编码 SEQ ID NO：3从核苷酸1到核苷酸1611.木葡聚糖酶是有用的，例如在清洁组合物和纤维素纤维的处理中。

2. 发明授权

US07141403B2 Endo-beta-1,4-glucanases 有权
标题翻译：内-β-1,4-葡聚糖酶
公开(公告)号：US07141403B2
公开(公告)日：2006-11-28
申请号：US11044363
申请日：2005-01-26
申请人： Helle Outtrup , Martin Schulein, deceased , Torben Henriksen, legal representative , Mads Eskelund Bjornvad , Keith Gibson
发明人： Helle Outtrup , Martin Schulein, deceased , Torben Henriksen, legal representative , Mads Eskelund Bjornvad , Keith Gibson
IPC分类号： C12N9/24 , C12N9/42 , C12N15/74 , C12P19/04 , C07H21/04
CPC分类号： C11D3/386
摘要： The present invention relates to an enzyme exhibiting endo-beta-1,4-glucanase activity (EC 3.2.1.4), which is a) a polypeptide encoded by the DNA sequence of positions 1 to 2322 of SEQ ID NO: 1; b) a polypeptide produced by culturing a cell comprising the sequence of SEQ ID NO: 1 under conditions wherein the DNA sequence is expressed; c) an endo-beta-1,4-glucanase enzyme having a sequence of at least 97% identity to the amino acid sequence of position 1 to position 773 of SEQ ID NO: 2; and fragments thereof exhibiting endo-beta-1,4-glucanase activity, and d) a polypeptide having endo-beta-1,4-glucanase activity that is encoded by a polynucleotide that hybridizes with the nucleotide sequence shown in positions 1–2322 of SEQ ID NO: 1, is useful for detergent and textile applications.
摘要翻译：本发明涉及显示内β-1,4-葡聚糖酶活性的酶（EC 3.2.1.4），其是a）由SEQ ID NO：1的1至2322位的DNA序列编码的多肽; b）通过在表达DNA序列的条件下培养包含SEQ ID NO：1的序列的细胞产生的多肽; c）具有与SEQ ID NO：2的位置1至位置773的氨基酸序列具有至少97％同一性的序列的内切-β-1,4-葡聚糖酶; 和表现出内切-β-1,4-葡聚糖酶活性的片段，以及d）具有内切-β-1,4-葡聚糖酶活性的多肽，其由与SEQ ID NO：1-2322所示核苷酸序列杂交的多核苷酸编码 SEQ ID NO：1可用于洗涤剂和纺织品应用。

3. 发明授权

US6017749A Alkalophilic Bacillus sp. AC13 and protease, xylanase, cellulase obtainable therefrom 失效
标题翻译：嗜碱芽孢杆菌 AC13和蛋白酶，木聚糖酶，可从其获得的纤维素酶
公开(公告)号：US6017749A
公开(公告)日：2000-01-25
申请号：US21279
申请日：1998-02-10
申请人： Helle Outtrup , Claus Dambmann , Arne Agerlin Olsen , Henrik Bisg.ang.rd-Frantzen , Martin Schulein
发明人： Helle Outtrup , Claus Dambmann , Arne Agerlin Olsen , Henrik Bisg.ang.rd-Frantzen , Martin Schulein
IPC分类号： C11D3/386 , C12N1/21 , C12N9/24 , C12N9/42 , C12N9/54 , C12N15/55 , D21C5/00 , C12N9/26
CPC分类号： C12Y302/01032 , C11D3/386 , C11D3/38636 , C11D3/38645 , C12N9/2437 , C12N9/248 , C12N9/54 , C12R1/07 , C12Y302/01004 , C12Y302/01008 , D21C5/005
摘要： This invention relates to Alkalophilic Bacillus sp. AC13 microorganisms and cultures, and mutants and variants thereof, and to enzymes obtainable from the microorganism.
摘要翻译：本发明涉及嗜碱芽孢杆菌属 AC13微生物和培养物及其突变体和变体，以及可从微生物获得的酶。

4. 发明申请

US20050112749A1 ENDO-BETA-1,4-GLUCANASE FROM BACILLUS 有权
标题翻译：来自BACILLUS的内切-14-GLUCANASE
公开(公告)号：US20050112749A1
公开(公告)日：2005-05-26
申请号：US10479446
申请日：2002-06-06
申请人： Helle Outtrup , Martin Schulein , Mads Eskelund , Keith Gibson
发明人： Helle Outtrup , Martin Schulein , Mads Eskelund , Keith Gibson
IPC分类号： C11D3/386 , C12N9/24 , C07H21/04 , C12N15/74 , C12P19/04
CPC分类号： C11D3/386
摘要： An enzyme exhibiting endo-beta-1,4-glucanase activity (EC 3.2.1.4), which is selected from one of a) a polypeptide encoded by the DNA sequence of positions 1 to 2322 of SEQ ID NO:1; b) a polypeptide produced by culturing a cell comprising the sequence of SEQ ID NO:1 under conditions wherein the DNA sequence is expressed; c) an endo-beta-1,4-glucanase enzyme having a sequence of at least 97% identity to the amino acid sequence of position 1 to position 773 of SEQ ID NO:2; and fragments thereof exhibiting endo-beta-1,4-glucanase activity, and d) a polypeptide having endo-beta-1,4-glucanase activity that is encoded by a polynucleo-tide that hybridizes with the nucleotide sequence shown in positions 1-2322 of SEQ ID NO:1, is useful for detergent and textile applications.
摘要翻译：显示内β-1,4-葡聚糖酶活性的酶（EC 3.2.1.4），其选自a）由SEQ ID NO：1的1至2322位的DNA序列编码的多肽; b）通过在表达DNA序列的条件下培养包含SEQ ID NO：1的序列的细胞产生的多肽; c）具有与SEQ ID NO：2的位置1至位置773的氨基酸序列具有至少97％同一性的序列的内切-β-1,4-葡聚糖酶; 和表现出内切-β-1,4-葡聚糖酶活性的片段，以及d）具有内切-β-1,4-葡聚糖酶活性的多肽，其与通过多核苷酸编码的多核苷酸杂交，所述多核苷酸与位置1- SEQ ID NO：1的2322可用于洗涤剂和纺织品应用。

5. 发明授权

US5770424A DNA constructs and methods of producing xylanolytic enzymes 失效
标题翻译： DNA构建体和生产木聚糖酶的方法
公开(公告)号：US5770424A
公开(公告)日：1998-06-23
申请号：US698978
申请日：1996-08-16
申请人： Helle Outtrup , Claus Dambmann , Arne Agerlin Olsen , Henrik Bisg.ang.rd-Frantzen , Martin Schulein , Per Linaa Jorgensen
发明人： Helle Outtrup , Claus Dambmann , Arne Agerlin Olsen , Henrik Bisg.ang.rd-Frantzen , Martin Schulein , Per Linaa Jorgensen
IPC分类号： C12N9/24 , C12N9/42 , C07H21/04 , C12N1/20 , C12N15/00
CPC分类号： C12Y302/01032 , C12N9/248 , C12N9/2482 , C12R1/07 , C12Y302/01008
摘要： The present invention is directed to isolated nucleic acid constructs comprising a nucleic acid sequence encoding xylanolytic enzymes derived from a strain of Bacillus agaradherens, recombinant vectors and host cells comprising such constructs, and methods for obtaining xylanolytic enzymes.
摘要翻译：本发明涉及分离的核酸构建体，其包含编码源自芽孢杆菌属芽孢杆菌的重组载体和宿主细胞的木聚糖酶的核酸序列，以及获得木聚糖分解酶的方法。

6. 发明授权

US5741693A Alkalophilic bacillus SP. AC13 and protease, xylanase, cellulase obtainable therefrom 失效
标题翻译：嗜碱芽孢杆菌SP。 AC13和蛋白酶，木聚糖酶，可从其获得的纤维素酶
公开(公告)号：US5741693A
公开(公告)日：1998-04-21
申请号：US343600
申请日：1994-11-30
申请人： Helle Outtrup , Claus Dambmann , Arne Agerlin Olsen , Henrik Bisg.ang.rd-Frantzen , Martin Schulein
发明人： Helle Outtrup , Claus Dambmann , Arne Agerlin Olsen , Henrik Bisg.ang.rd-Frantzen , Martin Schulein
IPC分类号： C11D3/386 , C12N1/20 , C12N9/24 , C12N9/42 , C12N9/54 , C12R1/07 , C12S3/08 , D21C5/00 , D21C9/10 , C12N9/50
CPC分类号： C12Y302/01032 , C11D3/386 , C11D3/38636 , C11D3/38645 , C12N9/2437 , C12N9/248 , C12N9/54 , C12R1/07 , C12Y302/01004 , C12Y302/01008 , D21C5/005
摘要： The present invention relates to proteases obtained from a strain of Bacillus sp. AC13, and having (a) an apparent molecular weight of approximately 30 kD as determined by SDS-PAGE; (b) an isoelectric point of approximately 9.3 as determined by isoelectric focusing on LKB Ampholine PAG plates; (c) a pH optimum above 10 determined at 25.degree. C. with casein as substrate; (d) a temperature optimum in the range of 45.degree.-55.degree. C. determined at pH 9.5 with casein as substrate; and (e) immunochemical properties identical or partially identical to those of a protease derived from Bacillus sp. AC13, NCIMB No. 40482.
摘要翻译： PCT No.PCT / DK93 / 00218 Sec。 371日期1994年11月30日 102（e）日期1994年11月30日PCT提交1993年7月2日PCT公布。公开号WO94 / 01532 1994年1月20日的日期本发明涉及从芽孢杆菌属菌株获得的蛋白酶。 AC13，并且具有（a）通过SDS-PAGE测定的约30kD的表观分子量; （b）通过等电聚焦在LKB两性苯胺PAG板上测定的约9.3的等电点; （c）在25℃下用酪蛋白作为底物测定的pH最佳值大于10; （d）在pH 9.5下测定最适温度为45-55℃的温度，酪蛋白作为底物; 和（e）与衍生自芽孢杆菌属的蛋白酶的免疫化学特性相同或部分相同的免疫化学性质。 AC13，NCIMB No.40482。

7. 发明申请

US20050215450A1 Endo-beta-1,4-glucanases 有权
标题翻译：内-β-1,4-葡聚糖酶
公开(公告)号：US20050215450A1
公开(公告)日：2005-09-29
申请号：US11044363
申请日：2005-01-26
申请人： Helle Outtrup , Martin Schulein , Torben Henriksen , Mads Bjornvad , Keith Gibson
发明人： Helle Outtrup , Martin Schulein , Torben Henriksen , Mads Bjornvad , Keith Gibson
IPC分类号： C11D3/386 , C07H21/04 , C12N1/21 , C12N9/42 , C12N15/74 , C12Q1/68
CPC分类号： C11D3/386
摘要： The present invention relates to an enzyme exhibiting endo-beta-1,4-glucanase activity (EC 3.2.1.4), which is a) a polypeptide encoded by the DNA sequence of positions 1 to 2322 of SEQ ID NO: 1; b) a polypeptide produced by culturing a cell comprising the sequence of SEQ ID NO: 1 under conditions wherein the DNA sequence is expressed; c) an endo-beta-1,4-glucanase enzyme having a sequence of at least 97% identity to the amino acid sequence of position 1 to position 773 of SEQ ID NO: 2; and fragments thereof exhibiting endo-beta-1,4-glucanase activity, and d) a polypeptide having endo-beta-1,4-glucanase activity that is encoded by a polynucleotide that hybridizes with the nucleotide sequence shown in positions 1-2322 of SEQ ID NO: 1, is useful for detergent and textile applications.
摘要翻译：本发明涉及显示内β-1,4-葡聚糖酶活性的酶（EC 3.2.1.4），其是a）由SEQ ID NO：1的1至2322位的DNA序列编码的多肽; b）通过在表达DNA序列的条件下培养包含SEQ ID NO：1的序列的细胞产生的多肽; c）具有与SEQ ID NO：2的位置1至位置773的氨基酸序列具有至少97％同一性的序列的内切-β-1,4-葡聚糖酶; 和表现出内切-β-1,4-葡聚糖酶活性的片段，以及d）具有内切-β-1,4-葡聚糖酶活性的多肽，其由与SEQ ID NO：1-2322所示核苷酸序列杂交的多核苷酸编码 SEQ ID NO：1可用于洗涤剂和纺织品应用。

8. 发明授权

US5945327A DNA constructs and methods of producing cellulytic enzymes 失效
公开(公告)号：US5945327A
公开(公告)日：1999-08-31
申请号：US870180
申请日：1997-06-06
申请人： Helle Outtrup , Claus Dambmann , Arne Agerlin Olsen , Henrik Bisg.ang.rd-Frantzen , Martin Schulein , Per Linaa Jorgensen , Mads E. Bjoernvad
发明人： Helle Outtrup , Claus Dambmann , Arne Agerlin Olsen , Henrik Bisg.ang.rd-Frantzen , Martin Schulein , Per Linaa Jorgensen , Mads E. Bjoernvad
IPC分类号： C11D3/386 , C12N1/21 , C12N9/24 , C12N9/42 , C12N9/54 , D21C5/00 , C07H21/04 , C12N15/55
CPC分类号： C12Y302/01032 , C11D3/386 , C11D3/38636 , C11D3/38645 , C12N9/2437 , C12N9/248 , C12N9/54 , C12R1/07 , C12Y302/01004 , C12Y302/01008 , D21C5/005 , Y10S435/832
摘要： An isolated nucleic acid constructs encoding cellulytic enzymes derived from a strain of Bacillus agaradherens, recombinant vectors and host cells comprising such constructs, and methods for obtaining cellulytic enzymes.

9. 发明授权

US5696068A Alkalophilic Bacillus sp. AC13 and protease, xylanase, cellulase obtainable therefrom 失效
标题翻译：嗜碱芽孢杆菌 AC13和蛋白酶，木聚糖酶，可从其获得的纤维素酶
公开(公告)号：US5696068A
公开(公告)日：1997-12-09
申请号：US469047
申请日：1995-06-06
申请人： Helle Outtrup , Claus Dambmann , Arne Agerlin Olsen , Henrik Bisg.ang.rd-Frantzen , Martin Schulein
发明人： Helle Outtrup , Claus Dambmann , Arne Agerlin Olsen , Henrik Bisg.ang.rd-Frantzen , Martin Schulein
IPC分类号： C11D3/386 , C12N1/20 , C12N9/24 , C12N9/42 , C12N9/54 , C12R1/07 , C12S3/08 , D21C5/00 , D21C9/10
CPC分类号： C12Y302/01032 , C11D3/386 , C11D3/38636 , C11D3/38645 , C12N9/2437 , C12N9/248 , C12N9/54 , C12R1/07 , C12Y302/01004 , C12Y302/01008 , D21C5/005
摘要： The present invention relates to novel microorganisms, novel enzymes obtainable herefrom, and to a method of producing the novel enzymes. More specifically, the invention relates to novel enzymes obtainable from strains of the novel alkalophilic species Bacillus sp. AC13. Moreover, the invention relates to a method for producing the enzymes of the invention, and to the use of the enzymes in detergents or in the paper pulp industry.
摘要翻译：本发明涉及新的微生物，可从其获得的新型酶以及生产新型酶的方法。更具体地，本发明涉及可从新型嗜碱性芽孢杆菌属菌株获得的新型酶。 AC13。此外，本发明涉及本发明的酶的制备方法，以及在洗涤剂或造纸工业中使用酶的方法。

10. 发明授权

US5981253A Alkalophilic Bacillus sp. AC13 and protease, xylanase, cellulase obtainable therefrom 失效
标题翻译：嗜碱芽孢杆菌 AC13和蛋白酶，木聚糖酶，可从其获得的纤维素酶
公开(公告)号：US5981253A
公开(公告)日：1999-11-09
申请号：US470398
申请日：1995-06-06
申请人： Helle Outtrup , Claus Dambmann , Arne Agerlin Olsen , Henrik Bisg.ang.rd-Frantzen , Martin Schulein
发明人： Helle Outtrup , Claus Dambmann , Arne Agerlin Olsen , Henrik Bisg.ang.rd-Frantzen , Martin Schulein
IPC分类号： C11D3/386 , C12N1/21 , C12N9/24 , C12N9/42 , C12N9/54 , C12N15/55 , D21C5/00 , C12N9/26
CPC分类号： C12Y302/01032 , C11D3/386 , C11D3/38636 , C11D3/38645 , C12N9/2437 , C12N9/248 , C12N9/54 , C12R1/07 , C12Y302/01004 , C12Y302/01008 , D21C5/005
摘要： The present invention relates to novel microorganisms, novel enzymes obtainable herefrom, and to a method of producing the novel enzymes. More specifically, the invention relates to novel enzymes obtainable from strains of the novel alkalophilic species Bacillus sp. AC13. Moreover, the invention relates to a method for producing the enzymes of the invention, and to the use of the enzymes in detergents or in the paper pulp industry.
摘要翻译：本发明涉及新的微生物，可从其获得的新型酶以及生产新型酶的方法。更具体地，本发明涉及可从新型嗜碱性芽孢杆菌属菌株获得的新型酶。 AC13。此外，本发明涉及本发明的酶的制备方法，以及在洗涤剂或造纸工业中使用酶的方法。

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