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1. 发明授权

US07832143B2 High throughput methods for sampling seeds 有权
标题翻译：用于采样种子的高通量方法
公开(公告)号：US07832143B2
公开(公告)日：2010-11-16
申请号：US12358985
申请日：2009-01-23
申请人： Kevin L. Deppermann , Michael W. Petersen , Allen T. Christian , James J. Schaefer , Chad Allen Stendal
发明人： Kevin L. Deppermann , Michael W. Petersen , Allen T. Christian , James J. Schaefer , Chad Allen Stendal
IPC分类号： A01B79/00 , A01B79/02 , A01C1/00 , A01G1/00 , A01H3/00
CPC分类号： A01H1/02 , A01H1/04 , A01H5/10 , C12N15/8241 , C12Q1/6895 , C12Q2600/156
摘要： A high-throughput method is provided for analyzing individual seeds in a population of seeds. The method generally includes removing a tissue sample from at least one or more seeds in the population of seeds while preserving germination viability of the at least one or more seeds from which the tissue sample is taken, depositing the tissue sample into an individual compartment of a sample tray, contacting the tissue sample with an extraction buffer to remove DNA from the sample, and analyzing the extracted DNA for the presence or absence of a transgene of interest. The extraction buffer may be present in the individual compartment of the sample tray before the tissue sample is deposited into the individual compartment.
摘要翻译：提供了一种用于分析种子群体中的个体种子的高通量方法。该方法通常包括从种子群体中的至少一种或多种种子中去除组织样品，同时保持所述至少一种或多种种子的萌发活力，所述至少一种或多种种子从其中取出组织样品，将组织样品沉积到单个隔室中样品盘，将组织样品与提取缓冲液接触以从样品中除去DNA，并分析所提取的DNA是否存在感兴趣的转基因。在将组织样品沉积到单独隔室中之前，提取缓冲液可存在于样品盘的单独隔室中。

2. 发明申请

US20090215060A1 HIGH THROUGHPUT METHODS FOR SAMPLING SEEDS 有权
标题翻译：用于采摘种子的高通量方法
公开(公告)号：US20090215060A1
公开(公告)日：2009-08-27
申请号：US12358985
申请日：2009-01-23
申请人： Kevin L. Deppermann , Allen T. Christian , James J. Schaefer , Chad Allen Stendal
发明人： Kevin L. Deppermann , Allen T. Christian , James J. Schaefer , Chad Allen Stendal
IPC分类号： C12Q1/68
CPC分类号： A01H1/02 , A01H1/04 , A01H5/10 , C12N15/8241 , C12Q1/6895 , C12Q2600/156
摘要： A high-throughput method is provided for analyzing individual seeds in a population of seeds. The method generally includes removing a tissue sample from at least one or more seeds in the population of seeds while preserving germination viability of the at least one or more seeds from which the tissue sample is taken, depositing the tissue sample into an individual compartment of a sample tray, contacting the tissue sample with an extraction buffer to remove DNA from the sample, and analyzing the extracted DNA for the presence or absence of a transgene of interest. The extraction buffer may be present in the individual compartment of the sample tray before the tissue sample is deposited into the individual compartment.
摘要翻译：提供了一种用于分析种子群体中的个体种子的高通量方法。该方法通常包括从种子群体中的至少一种或多种种子中去除组织样品，同时保持所述至少一种或多种种子的萌发活力，所述至少一种或多种种子从其中取出组织样品，将组织样品沉积到单个隔室中样品盘，将组织样品与提取缓冲液接触以从样品中除去DNA，并分析所提取的DNA是否存在感兴趣的转基因。在将组织样品沉积到单独隔室中之前，提取缓冲液可存在于样品盘的单独隔室中。

3. 发明授权

US09464329B2 Portable systems and methods for amplifying nucleotides and detecting nucleotide sequences 有权
标题翻译：用于扩增核苷酸和检测核苷酸序列的便携式系统和方法
公开(公告)号：US09464329B2
公开(公告)日：2016-10-11
申请号：US13637303
申请日：2011-03-17
申请人： Edward J. Cargill , Kevin L. Deppermann , Brad D. White , William A. Grote , Elias J. Yannakakis , Andrew M. Singleton , G. David Grothaus , Tiffany L. Stephans , Allen T. Christian , Donald W. Mittanck , Yelena A. Dudin
发明人： Edward J. Cargill , Kevin L. Deppermann , Brad D. White , William A. Grote , Elias J. Yannakakis , Andrew M. Singleton , G. David Grothaus , Tiffany L. Stephans , Allen T. Christian , Donald W. Mittanck , Yelena A. Dudin , Ramesh Govinthasamy
IPC分类号： C12Q1/68 , G01N21/64
CPC分类号： C12Q1/6895 , C12Q1/6844 , C12Q2600/13 , G01N21/643 , G01N21/645 , G01N2021/6421 , G01N2021/6432 , G01N2021/6441 , G01N2201/0221 , G01N2201/024 , G01N2201/062 , G01N2201/0627
摘要： Portable systems and methods for amplifying nucleotides and for detecting nucleotide sequences in a sample are provided. The portable instruments and methods use RPA techniques for DNA amplification and detect sample fluorescence in response to amplification and/or to the presence of specific DNA sequences.
摘要翻译：提供了用于扩增核苷酸和检测样品中核苷酸序列的便携式系统和方法。便携式仪器和方法使用RPA技术进行DNA扩增，并响应于扩增和/或特定DNA序列的存在而检测样品荧光。

4. 发明申请

US20150203922A1 Portable Systems and Methods for Amplifying Nucleotides and Detecting Nucleotide Sequences 有权
标题翻译：用于扩增核苷酸并检测核苷酸序列的便携式系统和方法
公开(公告)号：US20150203922A1
公开(公告)日：2015-07-23
申请号：US13637303
申请日：2011-03-17
申请人： Edward J. Cargill , Kevin L. Deppermann , Brad D. White , William A. Grote , Elias J. Yannakakis , Andrew M. Singleton , D. David Grothaus , Tiffany L. Stephans , Allen T. Christian , Donald W. Mittanck , Yelena A. Dudin , Ramesh Govinthasamy
发明人： Edward J. Cargill , Kevin L. Deppermann , Brad D. White , William A. Grote , Elias J. Yannakakis , Andrew M. Singleton , D. David Grothaus , Tiffany L. Stephans , Allen T. Christian , Donald W. Mittanck , Yelena A. Dudin , Ramesh Govinthasamy
IPC分类号： C12Q1/68 , G01N21/64
CPC分类号： C12Q1/6895 , C12Q1/6844 , C12Q2600/13 , G01N21/643 , G01N21/645 , G01N2021/6421 , G01N2021/6432 , G01N2021/6441 , G01N2201/0221 , G01N2201/024 , G01N2201/062 , G01N2201/0627
摘要： Portable systems and methods for amplifying nucleotides and for detecting nucleotide sequences in a sample are provided. The portable instruments and methods use RPA techniques for DNA amplification and detect sample fluorescence in response to amplification and/or to the presence of specific DNA sequences.
摘要翻译：提供了用于扩增核苷酸和检测样品中核苷酸序列的便携式系统和方法。便携式仪器和方法使用RPA技术进行DNA扩增，并响应于扩增和/或特定DNA序列的存在而检测样品荧光。

5. 发明申请

US20130045166A1 Methods of Polynucleotide Detection 有权
标题翻译：多核苷酸检测方法
公开(公告)号：US20130045166A1
公开(公告)日：2013-02-21
申请号：US13501155
申请日：2010-10-15
申请人： Ryan K. Bartlett , Allen T. Christian , Mingqi Deng , Sonya J. Franklin , Larry A. Gilbertson , G. David Grothaus , R. Douglas Sammons , Jianping Xu
发明人： Ryan K. Bartlett , Allen T. Christian , Mingqi Deng , Sonya J. Franklin , Larry A. Gilbertson , G. David Grothaus , R. Douglas Sammons , Jianping Xu
IPC分类号： G01N21/64 , A61K49/00
CPC分类号： C12Q1/6844 , C12Q2543/101
摘要： The present invention provides methods of detecting for the presence of a polynucleotide in vivo. These methods are particularly useful for performing identification and/or analysis of samples or specimens in which it is impossible, impractical, or undesirable to move or remove them from their current environment. Methods of practicing the present invention for the purpose of identifying and/or analyzing transgenic plant tissue or cells, in addition to animal tissue or cells and bacterial cells are also provided.
摘要翻译：本发明提供了在体内检测多核苷酸的存在的方法。这些方法对于进行样品或样品的鉴定和/或分析特别有用，在样品或样品中，不可能，不切实际或不希望将其从当前环境中移出或移除。还提供了用于鉴定和/或分析转基因植物组织或细胞（除动物组织或细胞和细菌细胞）之外的实施本发明的方法。

6. 发明申请

US20110027874A1 Packed Bed for Nucleic Acid Capture and Amplification 审中-公开
标题翻译：用于核酸捕获和扩增的包装床
公开(公告)号：US20110027874A1
公开(公告)日：2011-02-03
申请号：US12899924
申请日：2010-10-07
申请人： Elizabeth K. Wheeler , Christopher G. Bailey , Allen T. Christian
发明人： Elizabeth K. Wheeler , Christopher G. Bailey , Allen T. Christian
IPC分类号： C12M1/00
CPC分类号： C12Q1/6834 , C12Q1/686 , C12Q2547/101 , C12Q2531/113 , C12Q2565/518
摘要： A system for nucleic acid capture and amplification comprising introducing a sample potentially containing the nucleic acid into a packed bed wherein the nucleic acid adheres to the packed bed, introducing an amplification mix into the packed bed, and thermal cycling the packed bed and the nucleic acid between denaturation and annealing temperatures for PCR amplification. One embodiment provides an apparatus for DNA capture and amplification comprising a tubing or housing having a cavity, bed media in the cavity, and a heater operatively connected to the tubing or housing.
摘要翻译：一种用于核酸捕获和扩增的系统，包括将可能含有核酸的样品引入填充床中，其中核酸粘附到填充床，将扩增混合物引入填充床中，以及热循环填充床和核酸在变性和退火温度之间进行PCR扩增。一个实施例提供了一种用于DNA捕获和扩增的装置，其包括具有空腔的管或壳体，空腔中的床介质和可操作地连接到管道或壳体的加热器。

7. 发明授权

US06783943B2 Rolling circle amplification detection of RNA and DNA 失效
标题翻译： RNA和DNA的滚环扩增检测
公开(公告)号：US06783943B2
公开(公告)日：2004-08-31
申请号：US10032017
申请日：2001-12-18
申请人： Allen T. Christian , Melissa S. Pattee , Cristina M. Attix , James D. Tucker
发明人： Allen T. Christian , Melissa S. Pattee , Cristina M. Attix , James D. Tucker
IPC分类号： C12Q168
CPC分类号： C12Q1/6844 , C12Q1/6806 , C12Q2543/10 , C12Q2531/125 , C12Q2521/319 , C12Q2521/301
摘要： Rolling circle amplification (RCA) has been useful for detecting point mutations in isolated nucleic acids, but its application in cytological preparations has been problematic. By pretreating cells with a combination of restriction enzymes and exonucleases, we demonstrate RCA in solution and in situ to detect gene copy number and single base mutations. It can also detect and quantify transcribed RNA in individual cells, making it a versatile tool for cell-based assays.
摘要翻译：滚环扩增（RCA）已被用于检测分离的核酸中的点突变，但其在细胞学制备中的应用是有问题的。通过用限制酶和外切核酸酶的组合预处理细胞，我们在溶液中原位表达RCA，并原位检测基因拷贝数和单碱基突变。它还可以检测和定量单个细胞中的转录RNA，使其成为基于细胞的测定的通用工具。

8. 发明授权

US06432650B1 Amplification of chromosomal DNA in situ 失效
标题翻译：原位扩增染色体DNA
公开(公告)号：US06432650B1
公开(公告)日：2002-08-13
申请号：US09705471
申请日：2000-11-02
申请人： Allen T. Christian , Matthew A. Coleman , James D. Tucker
发明人： Allen T. Christian , Matthew A. Coleman , James D. Tucker
IPC分类号： C12Q168
CPC分类号： C12Q1/6876 , C12Q2600/142 , C12Q2600/156 , C12Q2600/158
摘要： Amplification of chromosomal DNA in situ to increase the amount of DNA associated with a chromosome or chromosome region is described. The amplification of chromosomal DNA in situ provides for the synthesis of Fluorescence in situ Hybridization (FISH) painting probes from single dissected chromosome fragments, the production of cDNA libraries from low copy mRNAs and improved in Comparative Genomic Hybridization (CGH) procedures.
摘要翻译：描述了原位扩增染色体DNA以增加染色体或染色体区域相关DNA的量。原位染色体DNA的扩增提供了来自单个解剖的染色体片段的荧光原位杂交（FISH）绘画探针的合成，从低拷贝mRNA产生cDNA文库并在比较基因组杂交（CGH）方法中得到改进。

9. 发明授权

US07871799B2 Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions 有权
标题翻译：在基于DNA聚合酶的合成反应中连续添加短DNA寡核苷酸
公开(公告)号：US07871799B2
公开(公告)日：2011-01-18
申请号：US10727779
申请日：2003-12-03
申请人： Shea N. Gardner , Raymond P. Mariella, Jr. , Allen T. Christian , Jennifer A. Young , David S. Clague
发明人： Shea N. Gardner , Raymond P. Mariella, Jr. , Allen T. Christian , Jennifer A. Young , David S. Clague
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6844 , C07H21/00 , C12N15/10 , C12N15/1027 , C12N15/1031 , C12Q1/6813 , C12Q1/686 , C12Q2521/101 , C12Q2533/107 , C12Q2525/143
摘要： A method of fabricating a DNA molecule of user-defined sequence. The method comprises the steps of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an even or odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths. In one embodiment starting sequence fragments are of different lengths, n, n+1, n+2, etc.
摘要翻译：制备用户定义序列的DNA分子的方法。该方法包括以下步骤：预先选择将包含用户定义序列的DNA分子的多个DNA序列片段，以及将多个DNA序列片段与至少一种聚合酶结合， DNA序列片段连接以产生用户定义序列的DNA分子。序列段可以是长度为n，其中n是偶或奇整数。在一个实施例中，期望的杂交重叠的长度由用户指定，并且用于组合它们的序列和协议由计算（生物信息学）预测指导。在一个实施例中，序列片段从多个读取框架组合以跨越序列的相同区域，使得可以以不同的重叠长度发生多个期望的杂交。在一个实施方案中，起始序列片段具有不同长度，n，n + 1，n + 2等

10. 发明授权

US07591197B2 Biobriefcase aerosol collector 失效
标题翻译： Biobriefcase气溶胶收集器
公开(公告)号：US07591197B2
公开(公告)日：2009-09-22
申请号：US11497918
申请日：2006-08-01
申请人： Perry M. Bell , Allen T. Christian , Christopher G. Bailey , Ladona Willis , Donald A. Masquelier , Shanavaz L. Nasarabadi
发明人： Perry M. Bell , Allen T. Christian , Christopher G. Bailey , Ladona Willis , Donald A. Masquelier , Shanavaz L. Nasarabadi
IPC分类号： G01N1/22
CPC分类号： G01N15/0606 , G01N1/2208 , G01N1/44 , G01N2015/0088 , G01N2015/0261
摘要： A system for sampling air and collecting particles entrained in the air that potentially include bioagents. The system comprises providing a receiving surface, directing a liquid to the receiving surface and producing a liquid surface. Collecting samples of the air and directing the samples of air so that the samples of air with particles entrained in the air impact the liquid surface. The particles potentially including bioagents become captured in the liquid. The air with particles entrained in the air impacts the liquid surface with sufficient velocity to entrain the particles into the liquid but cause minor turbulence. The liquid surface has a surface tension and the collector samples the air and directs the air to the liquid surface so that the air with particles entrained in the air impacts the liquid surface with sufficient velocity to entrain the particles into the liquid, but cause minor turbulence on the surface resulting in insignificant evaporation of the liquid.
摘要翻译：一种用于对空气进行取样并收集在空气中的颗粒的系统，其中可能包括生物标签。该系统包括提供接收表面，将液体引导到接收表面并产生液体表面。收集空气的样品并引导空气样品，使得夹带在空气中的颗粒的空气样品冲击液体表面。潜在地包括生物标记的颗粒被捕获在液体中。夹带在空气中的颗粒的空气以足够的速度冲击液体表面以将颗粒夹带入液体中，但是引起轻微的紊流。液体表面具有表面张力，并且收集器对空气进行采样并将空气引导到液体表面，使得夹带在空气中的颗粒的空气以足够的速度冲击液体表面以将颗粒夹带入液体中，但是引起轻微的紊流在表面上导致液体的微不足道的蒸发。

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