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1. 发明申请

US20140220598A1 BIOLOGICAL SUBSTANCE DETECTION METHOD 审中-公开
标题翻译：生物物质检测方法
公开(公告)号：US20140220598A1
公开(公告)日：2014-08-07
申请号：US14343411
申请日：2012-09-04
申请人： Kensaku Takanashi , Hisatake Okada , Yasushi Nakano , Kohsuke Gonda , Noriaki Ohuchi , Mika Watanabe
发明人： Kensaku Takanashi , Hisatake Okada , Yasushi Nakano , Kohsuke Gonda , Noriaki Ohuchi , Mika Watanabe
IPC分类号： G01N33/58 , G01N33/574
CPC分类号： G01N33/582 , G01N21/6428 , G01N33/56966 , G01N33/57492 , G01N2021/6439 , G01N2333/70596
摘要： The present invention provides a biological substance detection method for specifically detecting a biological substance from a pathological specimen, by which method, when immunostaining using a fluorescent label and staining for morphological observation using a staining agent for morphological observation are simultaneously performed, the results of fluorescence observation and immunostaining can be assessed properly even if the fluorescent label and/or the staining agent is/are deteriorated by irradiation with an excitation light. The biological substance detection method according to the present invention is characterized in that the brightness retention rate of an immunostained part is in a range of 80% to 120% in relation to the brightness retention rate of apart stained for morphological observation when the fluorescent label used for the immunostaining is observed.
摘要翻译：本发明提供一种用于特异性检测来自病理标本的生物物质的生物物质检测方法，通过该方法，当使用荧光标记物进行免疫染色并同时进行用于形态学观察的染色剂进行形态学观察的染色时，荧光的结果即使通过用激发光照射使荧光标记和/或染色剂劣化，也可以适当地评估观察和免疫染色。根据本发明的生物物质检测方法的特征在于，当使用荧光标记物时，免疫染色部分的亮度保持率相对于形态观察时分离染色的亮度保持率在80％至120％的范围内观察免疫染色。

2. 发明申请

US20140212889A1 METHOD FOR STAINING TISSUE 审中-公开
标题翻译：用于染色组织的方法
公开(公告)号：US20140212889A1
公开(公告)日：2014-07-31
申请号：US14342829
申请日：2012-09-04
申请人： Hideki Gouda , Hisatake Okada , Yasushi Nakano , Kohsuke Gonda , Noriaki Ohuchi , Mika Watanabe
发明人： Hideki Gouda , Hisatake Okada , Yasushi Nakano , Kohsuke Gonda , Noriaki Ohuchi , Mika Watanabe
IPC分类号： G01N33/569
CPC分类号： G01N33/569 , G01N1/30
摘要： It is an object of the present invention to provide a tissue staining method that makes it possible to observe both information on the morphology of a tissue and information on a biological substance such as an antigen molecule to be detected on a single section and in a single view field. The present invention provides a tissue staining method, including carrying out (A) a HE (hematoxylin-eosin) staining, and (B) a histochemical staining, serially on a single tissue section, wherein the histochemical staining is defined as a histochemical technique for detecting a biological substance to be detected in a tissue in a visible manner by use of a binding reaction between the biological substance to be detected and a probe biological substance capable of binding specifically to the biological substance to be detected.
摘要翻译：本发明的目的是提供一种组织染色方法，其可以在单个部分和单个部分上观察关于组织形态的信息和关于待检测的抗原分子的生物物质的信息查看字段。本发明提供一种组织染色方法，包括在单个组织切片上连续进行（A）HE（苏木精 - 伊红）染色和（B）组织化学染色，其中组织化学染色定义为组织化学技术通过使用要检测的生物物质和能够特异性结合待检测的生物物质的探针生物物质之间的结合反应，以可见的方式检测待检测的生物物质。

3. 发明授权

US10551378B2 Tissue staining method 有权
公开(公告)号：US10551378B2
公开(公告)日：2020-02-04
申请号：US14342829
申请日：2012-09-04
申请人： Hideki Gouda , Hisatake Okada , Yasushi Nakano , Kohsuke Gonda , Noriaki Ohuchi , Mika Watanabe
发明人： Hideki Gouda , Hisatake Okada , Yasushi Nakano , Kohsuke Gonda , Noriaki Ohuchi , Mika Watanabe
IPC分类号： G01N33/569
摘要： It is an object of the present invention to provide a tissue staining method that makes it possible to observe both information on the morphology of a tissue and information on a biological substance such as an antigen molecule to be detected on a single section and in a single view field. The present invention provides a tissue staining method, including carrying out (A) a HE (hematoxylin-eosin) staining, and (B) a histochemical staining, serially on a single tissue section, wherein the histochemical staining is defined as a histochemical technique for detecting a biological substance to be detected in a tissue in a visible manner by use of a binding reaction between the biological substance to be detected and a probe biological substance capable of binding specifically to the biological substance to be detected.

4. 发明申请

US20050229765A1 Information flow out preventing punch 审中-公开
标题翻译：信息流出防止冲孔
公开(公告)号：US20050229765A1
公开(公告)日：2005-10-20
申请号：US10518459
申请日：2003-06-25
申请人： Mika Watanabe
发明人： Mika Watanabe
IPC分类号： B26F1/32 , B26F1/36 , B26D1/12
CPC分类号： B26F1/36 , Y10T83/9387
摘要： An information flow out preventing punch for easily punching a hole in an address portion and a magnetic recording portion of a direct mail so that a third person cannot read those portions. The punch is small in size and light in weight and can be put into a drawer of a desk. The punch can punch not only paper but also a plastic card. Resources such as postcards after punched can easily be collected. This information flow out preventing punch includes an operation section and a punch blade group consisting of two or more punch blades. The punch blade group is moved by the operation section so as to punch a plurality of holes in a punch object. In the punch blade group, adjacent punch blades of n-th blade and n+1-th blade are in such a proximity that understanding of the information presented on the punch object becomes difficult.
摘要翻译：防止冲孔的信息流出，以便容易地冲压直邮的地址部分和磁记录部分中的孔，使得第三人不能读取这些部分。冲头尺寸小，重量轻，可以放在桌子的抽屉里。冲头不仅可以打纸，还可以打塑料卡。资源如打孔后的明信片可以轻松收集。该信息防止冲孔包括由两个或更多个冲压刀片组成的操作部和冲压刀组。冲压刀片组由操作部移动，以便在冲压件中冲压多个孔。在冲头刀片组中，第n刀片和第n + 1刀片的相邻冲头刀片靠近，使得对冲压件上呈现的信息的理解变得困难。

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