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1. 发明授权

US09062315B2 Method for transformation of stramenopile 有权
标题翻译：地幔的转化方法
公开(公告)号：US09062315B2
公开(公告)日：2015-06-23
申请号：US13877225
申请日：2011-09-30
申请人： Keishi Sakaguchi , Rie Hamaguchi , Takanori Matsuda , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yuji Okita , Shinichi Sugimoto
发明人： Keishi Sakaguchi , Rie Hamaguchi , Takanori Matsuda , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yuji Okita , Shinichi Sugimoto
IPC分类号： C12N15/79 , C12N15/89 , C12N9/02 , C12N9/10 , C12P7/64 , C12N15/52
CPC分类号： C12N15/79 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N15/113 , C12N15/52 , C12N15/895 , C12N2310/11 , C12N2310/14 , C12P7/6409 , C12P7/6427 , C12P7/6472 , C12Y114/19001 , C12Y114/19006 , C12Y203/01119
摘要： To provide a transformation method for producing a stramenopile organism having an improved unsaturated fatty acid production capability by disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner. [Solution] A method for transforming a stramenopile organism, which comprises disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner, and which is characterized in that the stramenopile organism is selected from Thraustochytrium aureum, Parietichytrium sarkarianum, Thraustochytrium roseum and Parietichytrium sp. and the gene to be disrupted or of which the expression is to be inhibited is a gene associated with the biosynthesis of a fatty acid.
摘要翻译：提供通过以遗传工程方式破坏该层状生物体的基因或抑制该基因的表达来生产具有改善的不饱和脂肪酸生产能力的前烧石生物的转化方法。 [解决方案]一种转基因生物的方法，其包括以基因工程方式破坏所述地精生物体的基因或抑制所述基因的表达，其特征在于，所述前列腺生物体选自甲状腺破囊壶菌（Salisochytrium aureum），，鱼（Parietichytrium sarkarianum），玫瑰色胸，和Parietichytrium sp。并且待破坏的基因或其表达被抑制的基因是与脂肪酸的生物合成相关的基因。

2. 发明申请

US20130309772A1 METHOD FOR TRANSFORMATION OF STRAMENOPILE 有权
标题翻译： STRAMENOPILE转换方法
公开(公告)号：US20130309772A1
公开(公告)日：2013-11-21
申请号：US13877225
申请日：2011-09-30
申请人： Keishi Sakaguchi , Rie Hamaguchi , Takanori Matsuda , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yuji Okita , Shinichi Sugimoto
发明人： Keishi Sakaguchi , Rie Hamaguchi , Takanori Matsuda , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yuji Okita , Shinichi Sugimoto
IPC分类号： C12N15/79 , C12N15/89
CPC分类号： C12N15/79 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N15/113 , C12N15/52 , C12N15/895 , C12N2310/11 , C12N2310/14 , C12P7/6409 , C12P7/6427 , C12P7/6472 , C12Y114/19001 , C12Y114/19006 , C12Y203/01119
摘要： To provide a transformation method for producing a stramenopile organism having an improved unsaturated fatty acid production capability by disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner. [Solution] A method for transforming a stramenopile organism, which comprises disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner, and which is characterized in that the stramenopile organism is selected from Thraustochytrium aureum, Parietichytrium sarkarianum, Thraustochytrium roseum and Parietichytrium sp. and the gene to be disrupted or of which the expression is to be inhibited is a gene associated with the biosynthesis of a fatty acid.
摘要翻译：提供通过以遗传工程方式破坏该层状生物体的基因或抑制该基因的表达来生产具有改善的不饱和脂肪酸生产能力的前烧石生物的转化方法。 [解决方案]一种转基因生物的方法，其包括以基因工程方式破坏所述地精生物体的基因或抑制所述基因的表达，其特征在于，所述前列腺生物体选自甲状腺破囊壶菌（Salisochytrium aureum），，鱼（Parietichytrium sarkarianum），玫瑰色胸，和Parietichytrium sp。并且待破坏的基因或其表达被抑制的基因是与脂肪酸的生物合成相关的基因。

3. 发明授权

US09150891B2 Method for transforming stramenopiles 有权
标题翻译：转化层状砷的方法
公开(公告)号：US09150891B2
公开(公告)日：2015-10-06
申请号：US13497894
申请日：2010-09-24
申请人： Keishi Sakaguchi , Takanori Matsuda , Takumi Kobayashi , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yosuke Taoka , Yuji Okita , Hitoshi Izumida , Shinichi Sugimoto
发明人： Keishi Sakaguchi , Takanori Matsuda , Takumi Kobayashi , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yosuke Taoka , Yuji Okita , Hitoshi Izumida , Shinichi Sugimoto
IPC分类号： C12P7/64 , C12P21/06 , C12N1/00 , C12N1/10 , C12N15/00 , C11C3/00 , C12N9/02 , C12N15/79 , C12N15/82
CPC分类号： C12P7/6409 , C11C3/00 , C12N9/0071 , C12N9/0083 , C12N15/79 , C12N15/8201 , C12N15/8247 , C12P7/6427
摘要： Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and/or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.
摘要翻译：公开了能够提高产生有机物的能力的转化方法。用于转化stramenopile的方法包括将外源基因转移到作为属于Labyrinthula类的微生物的地幔中，更具体地是属于Labyrinthula，Altornia，Aplanochytrium，Schizochytrium，Aurantiochytrium，Thraustochytrium，Ulkenia等。所述外源基因，其是与抗生素，比色蛋白质和/或脂肪酸去饱和酶（＆Dgr。5去饱和酶基因，＆Dgr; 12去饱和酶基因和/或ω3去饱和酶基因）的耐受相关的基因通过使用电穿孔或基因 - 枪技术。

4. 发明申请

US20120322116A1 METHOD FOR TRANSFORMING STRAMENOPILES 有权
标题翻译：改性方法
公开(公告)号：US20120322116A1
公开(公告)日：2012-12-20
申请号：US13497894
申请日：2010-09-24
申请人： Keishi Sakaguchi , Takanori Matsuda , Takumi Kobayashi , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yosuke Taoka , Yuji Okita , Hitoshi Izumida , Shinichi Sugimoto
发明人： Keishi Sakaguchi , Takanori Matsuda , Takumi Kobayashi , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yosuke Taoka , Yuji Okita , Hitoshi Izumida , Shinichi Sugimoto
IPC分类号： C12P7/64 , C12N15/79
CPC分类号： C12P7/6409 , C11C3/00 , C12N9/0071 , C12N9/0083 , C12N15/79 , C12N15/8201 , C12N15/8247 , C12P7/6427
摘要： Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and/or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.
摘要翻译：公开了能够提高产生有机物的能力的转化方法。用于转化stramenopile的方法包括将外源基因转移到作为属于Labyrinthula类的微生物的地幔中，更具体地是属于Labyrinthula，Altornia，Aplanochytrium，Schizochytrium，Aurantiochytrium，Thraustochytrium，Ulkenia等。所述外源基因，其是与抗生素，比色蛋白质和/或脂肪酸去饱和酶（＆Dgr。5去饱和酶基因，＆Dgr; 12去饱和酶基因和/或ω3去饱和酶基因）的耐受相关的基因通过使用电穿孔或基因 - 枪技术。

5. 发明申请

US20140121336A1 METHOD FOR INCREASING PRODUCTION VOLUME OF FINE PARTICLES USING FORCED THIN FILM FLUID TREATMENT APPARATUS 有权
标题翻译：使用强力薄膜流体处理装置增加微粒生产量的方法
公开(公告)号：US20140121336A1
公开(公告)日：2014-05-01
申请号：US14123051
申请日：2012-03-15
申请人： Masaki Maekawa , Kaeko Araki , Daisuke Honda , Masakazu Enomura
发明人： Masaki Maekawa , Kaeko Araki , Daisuke Honda , Masakazu Enomura
IPC分类号： B22F9/16 , C07D471/04 , C01G23/08
CPC分类号： B22F9/16 , B01F3/1221 , B01F7/00775 , B01F7/00791 , B01J19/1887 , B22F1/0018 , B22F1/0022 , B22F1/0025 , B22F9/24 , B22F2998/10 , B22F2999/00 , C01G23/053 , C01G23/08 , C01P2004/04 , C07D471/04 , C22C5/06
摘要： The present invention addresses the problem of providing a new method for increasing the production of fine particles. Using at least two types of fluids to be processed, a raw material fluid containing at least one type of fine particle raw material and a fluid for treating the fine particle raw material, fine particles are obtained by mixing the fluids to be processed in a thin film fluid formed between at least two processing surfaces which are disposed to be faced with each other so as to be able to approach to and separate from each other, at least one of which rotates relative to the other. At this time, the production of the fine particles is increased by introducing the raw material fluid from the centers of the processing surfaces.
摘要翻译：本发明解决了提供用于增加细颗粒的生产的新方法的问题。使用至少两种类型的待处理流体，含有至少一种类型的细粒原料的原料流体和用于处理细粒原料的流体，通过将待处理的流体以薄的在至少两个处理表面之间形成的薄膜流体，所述至少两个处理表面被设置成彼此面对，以便能够彼此接近和分离，其中至少一个相对于另一个旋转。此时，通过从处理用面的中心引入原料液，能够提高微粒的制造。

6. 发明申请

US20140037519A1 METHOD FOR PRODUCING OXIDE/HYDROXIDE 有权
标题翻译：生产氧化物/氢氧化物的方法
公开(公告)号：US20140037519A1
公开(公告)日：2014-02-06
申请号：US14112887
申请日：2011-04-28
申请人： Jun Kuraki , Masaki Maekawa , Daisuke Honda , Masakazu Enomura
发明人： Jun Kuraki , Masaki Maekawa , Daisuke Honda , Masakazu Enomura
IPC分类号： C22B19/34 , C01G9/03
CPC分类号： C22B19/34 , B01F3/0807 , B01F7/00775 , B01F7/00791 , B01F15/0203 , B01F15/0243 , B01F15/0291 , B82Y30/00 , C01B13/32 , C01B13/34 , C01G9/02 , C01G9/03 , C01P2002/01 , C01P2002/72 , C01P2002/88 , C01P2004/04 , C01P2004/62 , C01P2004/64
摘要： Provided is a method for producing an oxide and/or hydroxide wherein the ratio of oxide and hydroxide has been controlled. The method produces an oxide, a hydroxide, or a mixture thereof, and obtains an oxide and/or a hydroxide wherein the ratio of oxide and hydroxide has been controlled by means of changing a specific condition relating to at least one fluid to be processed introduced between processing surfaces (1, 2) when causing the precipitation of the oxide, hydroxide, or mixture thereof by mixing an basic fluid containing at least one type of basic substance and a fluid containing at least one type of metal or metallic substance as the fluids to be processed between the processing surfaces (1, 2) that are provided facing each other, are able to approach to and separate from each other, and of which at least one rotates relative to the other. The specific condition is at least one condition selected from the group consisting of: the speed of introduction of at least one of the fluids to be processed; and the pH of at least one of the fluids to be processed.
摘要翻译：提供一种氧化物和/或氢氧化物的制造方法，其中氧化物和氢氧化物的比例已被控制。该方法产生氧化物，氢氧化物或其混合物，并获得氧化物和/或氢氧化物，其中氧化物和氢氧化物的比例已经通过改变与至少一种待处理流体相关的特定条件来控制当通过混合含有至少一种碱性物质的碱性流体和含有至少一种类型的金属或金属物质的流体作为流体而使氧化物，氢氧化物或其混合物沉淀时，处理表面（1,2）之间在彼此面对地设置的处理表面（1,2）之间进行处理，能够彼此接近并分离，并且至少一个相对于另一个旋转。所述具体条件是选自以下的至少一种条件：至少一种待处理流体的引入速度; 和至少一种要处理的流体的pH。

7. 发明申请

US20130220180A1 SOLID SOLUTION PIGMENT NANOPARTICLES AND METHOD FOR PRODUCING SOLID SOLUTION PIGMENT NANOPARTICLES HAVING CONTROLLED SOLID SOLUTION RATIO 有权
标题翻译：固体溶剂颜料纳米粒子及其制备具有控制固体溶液比例的固体溶解性颜料纳米粒子的方法
公开(公告)号：US20130220180A1
公开(公告)日：2013-08-29
申请号：US13883692
申请日：2011-05-20
申请人： Masaki Maekawa , Kaeko Araki , Hideyuki Oka , Daisuke Honda , Masakazu Enomura
发明人： Masaki Maekawa , Kaeko Araki , Hideyuki Oka , Daisuke Honda , Masakazu Enomura
IPC分类号： C09C1/62
CPC分类号： C07F3/06 , B01D11/0269 , B01F3/0861 , B82Y30/00 , C01P2004/64 , C01P2004/82 , C07F1/08 , C09B67/0017 , C09B67/0019 , C09B67/0026 , C09B67/0035 , C09B67/0092 , C09C1/0081 , C09C1/04 , C09C1/627
摘要： The problem addressed by the present invention is to provide; solid solution pigment nanoparticles having a homogeneous solid solution ratio; a method for producing solid solution pigment nanoparticles having a homogeneous solid solution ratio in each primary particle; and a method for controlling the solid solution ratio of solid solution pigment nanoparticles. The solid solution pigment nanoparticles are prepared by precipitating at least two types of pigment by mixing a pigment precipitation solvent and; at least one type of pigment solution wherein at least two types of pigment are dissolved in a solvent: or at least two types of pigment solution wherein at least one type of pigment is dissolved in a solvent. The solid solution pigment nanoparticles are wherein the solid solution ratio of the at least two types of pigment in the primary particles of the precipitated solid solution pigment nanoparticles with respect to the ratio of the at least two types of pigment in the pigment solution mixed with the pigment precipitation solvent having a precision within 25%.
摘要翻译：本发明提出的问题在于：具有均匀固溶比的固溶颜料纳米粒子; 一种在一次粒子中具有均匀固溶比的固溶颜料纳米粒子的制造方法; 以及控制固溶颜料纳米粒子的固溶比的方法。固溶颜料纳米粒子是通过混合颜料沉淀溶剂和至少两种颜料沉淀来制备的; 至少一种类型的颜料溶液，其中至少两种类型的颜料溶解在溶剂中：或至少两种类型的颜料溶液，其中至少一种类型的颜料溶解在溶剂中。固溶颜料纳米粒子，其中沉淀的固溶颜料纳米颗粒的初级颗粒中的至少两种类型的颜料的固溶比相对于颜料溶液中的至少两种类型的颜料与颜料沉淀溶剂的精度在25％以内。

8. 发明授权

US09580447B2 Highly heat-resistant phthalocyanine 有权
标题翻译：高耐热酞菁
公开(公告)号：US09580447B2
公开(公告)日：2017-02-28
申请号：US13989082
申请日：2011-11-22
申请人： Masaki Maekawa , Daisuke Honda , Masakazu Enomura
发明人： Masaki Maekawa , Daisuke Honda , Masakazu Enomura
IPC分类号： C07D487/22 , C07F3/06 , B01D11/02 , B01F3/08 , C09B67/14 , C09B67/22 , C09C1/00 , C09C1/62 , C09C1/04 , C07F1/08 , B82Y30/00 , C09B67/42 , C09B67/16 , C09B67/50
CPC分类号： C07F3/06 , B01D11/0269 , B01F3/0861 , B82Y30/00 , C01P2004/64 , C01P2004/82 , C07F1/08 , C09B67/0017 , C09B67/0019 , C09B67/0026 , C09B67/0035 , C09B67/0092 , C09C1/0081 , C09C1/04 , C09C1/627
摘要： The problem addressed by the present invention is to provide a high heat-resistant phthalocyanine. The phthalocyanine is separated by mixing a phthalocyanine separation solvent and a phthalocyanine solution wherein a phthalocyanine starting material is dissolved in a solvent. The phthalocyanine is wherein having high heat resistance, the decomposition temperature of the separated phthalocyanine being at least 10° C. higher than the decomposition temperature of the phthalocyanine starting material. Also, the phthalocyanine solution may be the result of dissolving at least two types of phthalocyanine starting material in the solvent, the separated phthalocyanine being wherein containing a solid solvent of the at least two types of phthalocyanine starting material and by the decomposition temperature of the separated phthalocyanine being at least 10° C. higher than the decomposition temperature of a mixture of at least two types of phthalocyanine separated by mixing the phthalocyanine separation solvent and each of at least two types of phthalocyanine solution resulting from dissolving each of the at least two types of phthalocyanine starting material in a solvent.
摘要翻译：本发明解决的问题是提供高耐热酞菁。通过混合酞菁分离溶剂和酞菁溶液将酞菁原料溶解在溶剂中来分离酞菁。酞菁具有高耐热性，分离的酞菁的分解温度比酞菁起始原料的分解温度高10℃以上。此外，酞菁溶液可能是将至少两种类型的酞菁原料溶解在溶剂中的结果，其中分离的酞菁含有至少两种类型的酞菁起始原料的固体溶剂和分离的酞菁比通过混合酞菁分离溶剂和由至少两种类型的酞菁溶液中分离出的至少两种类型的酞菁溶液中的至少两种类型的酞菁溶液的分解温度高至少两种类型的酞菁的分解温度高至少10℃ 的酞菁原料在溶剂中。

9. 发明授权

US09249429B2 Microorganism having high squalene-producing ability, and method for producing squalene by means of same 有权
标题翻译：具有高角鲨烯生产能力的微生物和通过其生产角鲨烯的方法
公开(公告)号：US09249429B2
公开(公告)日：2016-02-02
申请号：US13992638
申请日：2011-12-09
申请人： Makoto Watanabe , Kunimitsu Kaya , Makato Shiho , Atsushi Nakazawa , Isao Inoue , Daisuke Honda
发明人： Makoto Watanabe , Kunimitsu Kaya , Makato Shiho , Atsushi Nakazawa , Isao Inoue , Daisuke Honda
IPC分类号： C12P5/02 , C12R1/89
CPC分类号： C12P5/026 , C12R1/89
摘要： Provided is a strain belonging to the genus Aurantiochytrium, the strain being characterized by there being at least 10 mass % of squalene in all the lipids produced by the strain, and by forming colonies exhibiting an orange-to-red color.
摘要翻译：本发明提供一种属于紫堇属的菌株，其特征在于，由菌株产生的所有脂质中含有至少10质量％的角鲨烯，并形成呈橙色至红色的菌落。

10. 发明授权

US09005567B2 Method for producing isolatable oxide microparticles or hydroxide microparticles 有权
标题翻译：生产可分离氧化物微粒或氢氧化物微粒的方法
公开(公告)号：US09005567B2
公开(公告)日：2015-04-14
申请号：US13818954
申请日：2011-08-26
申请人： Jun Kuraki , Kaeko Araki , Masaki Maekawa , Daisuke Honda , Masakazu Enomura
发明人： Jun Kuraki , Kaeko Araki , Masaki Maekawa , Daisuke Honda , Masakazu Enomura
IPC分类号： C01B13/14 , C01G23/047 , C01G23/053 , B01F7/00 , C01B13/36 , C01F5/02 , C01F5/20 , C01F5/14 , C01F17/00 , B01D9/00 , C01G25/02 , C01G1/02 , B82Y30/00
CPC分类号： C01G23/0536 , B01D9/0009 , B01F7/00775 , B01F7/00791 , B82Y30/00 , C01B13/36 , C01F5/02 , C01F5/14 , C01F5/20 , C01F17/0043 , C01G1/02 , C01G23/0532 , C01G25/02 , C01P2002/54 , C01P2004/04 , C01P2004/64
摘要： A method for producing isolatable oxide microparticles or hydroxide microparticles using an apparatus that processes a fluid between processing surfaces of processing members that are arranged opposite each other so as to be able to approach to or separate from each other and such that at least one can rotate relative to the other. At least two fluids are mixed and oxide microparticles or hydroxide microparticles are separated, said two fluids including: a fluid containing a microparticle raw material solution comprising a microparticle raw material mixed into a solvent, and a fluid containing a microparticle-separation solution. Immediately thereafter, the following are mixed to obtain isolatable oxide microparticles or hydroxide microparticles: a fluid containing the separated oxide microparticles or hydroxide microparticles; and a fluid containing a microparticle-treatment-substance-containing solution that contains a microparticle-treatment substance that adjusts the dispersibility of the separated oxide microparticles or hydroxide microparticles.
摘要翻译：一种用于制造可分离氧化物微粒或氢氧化物微粒的方法，该方法使用处理相互相对布置的处理构件的处理表面之间的流体以便能够彼此接近或分离并使得至少一个可旋转的装置相对于另一个。混合至少两种流体，分离氧化物微粒或氢氧化物微粒，所述两种流体包括：含有混合在溶剂中的微粒原料的微粒原料溶液的流体和含有微粒分离溶液的流体。之后，混合以下物质，得到可分离的氧化物微粒或氢氧化物微粒：含有分离的氧化物微粒或氢氧化物微粒的流体; 以及含有含微粒处理物质的溶液的流体，其含有调节分离的氧化物微粒或氢氧化物微粒的分散性的微粒处理物质。

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