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1. 发明申请

US20120322116A1 METHOD FOR TRANSFORMING STRAMENOPILES 有权
标题翻译：改性方法
公开(公告)号：US20120322116A1
公开(公告)日：2012-12-20
申请号：US13497894
申请日：2010-09-24
申请人： Keishi Sakaguchi , Takanori Matsuda , Takumi Kobayashi , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yosuke Taoka , Yuji Okita , Hitoshi Izumida , Shinichi Sugimoto
发明人： Keishi Sakaguchi , Takanori Matsuda , Takumi Kobayashi , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yosuke Taoka , Yuji Okita , Hitoshi Izumida , Shinichi Sugimoto
IPC分类号： C12P7/64 , C12N15/79
CPC分类号： C12P7/6409 , C11C3/00 , C12N9/0071 , C12N9/0083 , C12N15/79 , C12N15/8201 , C12N15/8247 , C12P7/6427
摘要： Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and/or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.
摘要翻译：公开了能够提高产生有机物的能力的转化方法。用于转化stramenopile的方法包括将外源基因转移到作为属于Labyrinthula类的微生物的地幔中，更具体地是属于Labyrinthula，Altornia，Aplanochytrium，Schizochytrium，Aurantiochytrium，Thraustochytrium，Ulkenia等。所述外源基因，其是与抗生素，比色蛋白质和/或脂肪酸去饱和酶（＆Dgr。5去饱和酶基因，＆Dgr; 12去饱和酶基因和/或ω3去饱和酶基因）的耐受相关的基因通过使用电穿孔或基因 - 枪技术。

2. 发明授权

US09150891B2 Method for transforming stramenopiles 有权
标题翻译：转化层状砷的方法
公开(公告)号：US09150891B2
公开(公告)日：2015-10-06
申请号：US13497894
申请日：2010-09-24
申请人： Keishi Sakaguchi , Takanori Matsuda , Takumi Kobayashi , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yosuke Taoka , Yuji Okita , Hitoshi Izumida , Shinichi Sugimoto
发明人： Keishi Sakaguchi , Takanori Matsuda , Takumi Kobayashi , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yosuke Taoka , Yuji Okita , Hitoshi Izumida , Shinichi Sugimoto
IPC分类号： C12P7/64 , C12P21/06 , C12N1/00 , C12N1/10 , C12N15/00 , C11C3/00 , C12N9/02 , C12N15/79 , C12N15/82
CPC分类号： C12P7/6409 , C11C3/00 , C12N9/0071 , C12N9/0083 , C12N15/79 , C12N15/8201 , C12N15/8247 , C12P7/6427
摘要： Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and/or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.
摘要翻译：公开了能够提高产生有机物的能力的转化方法。用于转化stramenopile的方法包括将外源基因转移到作为属于Labyrinthula类的微生物的地幔中，更具体地是属于Labyrinthula，Altornia，Aplanochytrium，Schizochytrium，Aurantiochytrium，Thraustochytrium，Ulkenia等。所述外源基因，其是与抗生素，比色蛋白质和/或脂肪酸去饱和酶（＆Dgr。5去饱和酶基因，＆Dgr; 12去饱和酶基因和/或ω3去饱和酶基因）的耐受相关的基因通过使用电穿孔或基因 - 枪技术。

3. 发明申请

US20130309772A1 METHOD FOR TRANSFORMATION OF STRAMENOPILE 有权
标题翻译： STRAMENOPILE转换方法
公开(公告)号：US20130309772A1
公开(公告)日：2013-11-21
申请号：US13877225
申请日：2011-09-30
申请人： Keishi Sakaguchi , Rie Hamaguchi , Takanori Matsuda , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yuji Okita , Shinichi Sugimoto
发明人： Keishi Sakaguchi , Rie Hamaguchi , Takanori Matsuda , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yuji Okita , Shinichi Sugimoto
IPC分类号： C12N15/79 , C12N15/89
CPC分类号： C12N15/79 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N15/113 , C12N15/52 , C12N15/895 , C12N2310/11 , C12N2310/14 , C12P7/6409 , C12P7/6427 , C12P7/6472 , C12Y114/19001 , C12Y114/19006 , C12Y203/01119
摘要： To provide a transformation method for producing a stramenopile organism having an improved unsaturated fatty acid production capability by disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner. [Solution] A method for transforming a stramenopile organism, which comprises disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner, and which is characterized in that the stramenopile organism is selected from Thraustochytrium aureum, Parietichytrium sarkarianum, Thraustochytrium roseum and Parietichytrium sp. and the gene to be disrupted or of which the expression is to be inhibited is a gene associated with the biosynthesis of a fatty acid.
摘要翻译：提供通过以遗传工程方式破坏该层状生物体的基因或抑制该基因的表达来生产具有改善的不饱和脂肪酸生产能力的前烧石生物的转化方法。 [解决方案]一种转基因生物的方法，其包括以基因工程方式破坏所述地精生物体的基因或抑制所述基因的表达，其特征在于，所述前列腺生物体选自甲状腺破囊壶菌（Salisochytrium aureum），，鱼（Parietichytrium sarkarianum），玫瑰色胸，和Parietichytrium sp。并且待破坏的基因或其表达被抑制的基因是与脂肪酸的生物合成相关的基因。

4. 发明授权

US09062315B2 Method for transformation of stramenopile 有权
标题翻译：地幔的转化方法
公开(公告)号：US09062315B2
公开(公告)日：2015-06-23
申请号：US13877225
申请日：2011-09-30
申请人： Keishi Sakaguchi , Rie Hamaguchi , Takanori Matsuda , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yuji Okita , Shinichi Sugimoto
发明人： Keishi Sakaguchi , Rie Hamaguchi , Takanori Matsuda , Makoto Ito , Naoki Nagano , Masahiro Hayashi , Daisuke Honda , Yuji Okita , Shinichi Sugimoto
IPC分类号： C12N15/79 , C12N15/89 , C12N9/02 , C12N9/10 , C12P7/64 , C12N15/52
CPC分类号： C12N15/79 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N15/113 , C12N15/52 , C12N15/895 , C12N2310/11 , C12N2310/14 , C12P7/6409 , C12P7/6427 , C12P7/6472 , C12Y114/19001 , C12Y114/19006 , C12Y203/01119
摘要： To provide a transformation method for producing a stramenopile organism having an improved unsaturated fatty acid production capability by disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner. [Solution] A method for transforming a stramenopile organism, which comprises disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner, and which is characterized in that the stramenopile organism is selected from Thraustochytrium aureum, Parietichytrium sarkarianum, Thraustochytrium roseum and Parietichytrium sp. and the gene to be disrupted or of which the expression is to be inhibited is a gene associated with the biosynthesis of a fatty acid.
摘要翻译：提供通过以遗传工程方式破坏该层状生物体的基因或抑制该基因的表达来生产具有改善的不饱和脂肪酸生产能力的前烧石生物的转化方法。 [解决方案]一种转基因生物的方法，其包括以基因工程方式破坏所述地精生物体的基因或抑制所述基因的表达，其特征在于，所述前列腺生物体选自甲状腺破囊壶菌（Salisochytrium aureum），，鱼（Parietichytrium sarkarianum），玫瑰色胸，和Parietichytrium sp。并且待破坏的基因或其表达被抑制的基因是与脂肪酸的生物合成相关的基因。

5. 发明申请

US20120100550A1 METHOD FOR DETERMINING FATTY ACID SYNTHESIS PATHWAY OF MICROORGANISM, AND PCR PRIMER SET FOR USE IN THE METHOD 审中-公开
标题翻译：用于确定微生物的脂肪酸合成途径的方法，以及用于该方法的PCR引物组
公开(公告)号：US20120100550A1
公开(公告)日：2012-04-26
申请号：US13254794
申请日：2010-03-04
申请人： Naoki Nagano , Masahiro Hayashi , Keishi Sakaguchi , Makoto Ito , Yosuke Taoka , Yuji Okita
发明人： Naoki Nagano , Masahiro Hayashi , Keishi Sakaguchi , Makoto Ito , Yosuke Taoka , Yuji Okita
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689
摘要： Disclosed is a method for determining the fatty acid synthesis pathway of a microorganism. Specifically disclosed is a method for determining the fatty acid synthesis pathway of a microorganism, which is characterized by producing degenerate primers for a fatty acid synthesis-related enzyme gene and determining the presence or absence of the fatty acid synthesis-related gene in the genome gene extracted from the microorganism using the degenerate primers. The sequences for degenerate primers for C20-elongase gene, which is a fatty acid synthesis-related enzyme gene, are ATHGARTWYTKBRTITTYGTICA (SEQ ID NO:1) and TARTRISWRTACATIADIAMRTG (SEQ ID NO:2), and the sequences for degenerate primers for ?4-desaturase gene are GGNCAYCAYCMITAYACNAA (SEQ ID NO:3) and TCDATYTGRTGIBWIARNCC (SEQ ID NO:4). The microorganism is one belonging to the class Labyrinthulea. Also specifically disclosed is a PCR primer set for use in the determination method. The method is useful as a method for screening a microorganism capable of synthesizing a fatty acid.
摘要翻译：公开了一种测定微生物的脂肪酸合成途径的方法。具体公开的是测定微生物的脂肪酸合成途径的方法，其特征在于制备脂肪酸合成相关酶基因的简并引物，并测定基因组基因中脂肪酸合成相关基因的存在或不存在使用简并引物从微生物中提取。作为脂肪酸合成相关酶基因的C20延伸酶基因的简并引物的序列是ATHGARTWYTKBRTITTYGTICA（SEQ ID NO：1）和TARTRISWRTACATIADIAMRTG（SEQ ID NO：2），并且用于Δ4的简并引物的序列 - 脱氢酶基因是GGNCAYCAYCMITAYACNAA（SEQ ID NO：3）和TCDATYTGRTGIBWIARNCC（SEQ ID NO：4）。该微生物属于Labyrinthulea类。还具体公开了用于确定方法的PCR引物组。该方法可用作筛选能够合成脂肪酸的微生物的方法。

6. 发明申请

US20070238147A1 Method of detecting foreign matter 审中-公开
标题翻译：异物检测方法
公开(公告)号：US20070238147A1
公开(公告)日：2007-10-11
申请号：US11727202
申请日：2007-03-23
申请人： Takashi Okamoto , Yuji Okita , Satoru Chiba
发明人： Takashi Okamoto , Yuji Okita , Satoru Chiba
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , G01N33/12 , G01N2333/37 , G06T7/0004 , G06T2207/30128
摘要： A method for detecting foreign matter such as a parasite, in particular, to provide a method for detecting parasitic on meat. A method for detecting foreign matter includes detecting fluorescence excited by irradiating the foreign matter such as a parasite with visible light. The method is suitable for detecting a meat parasite. A method for detecting a parasite includes selectively detecting fluorescence emitted from a parasite with a filter that does not transmit excitation light but that transmits fluorescence emitted from the parasite. An apparatus for detecting a parasite includes a light source that illuminates a specimen with a visible light having a predetermined wavelength, a filter that absorbs diffuse reflection of excitation light, a camera that shoots fluorescence from the specimen, an image processing unit, and a monitor.
摘要翻译：特别是用于检测诸如寄生虫的异物的方法，以提供一种检测肉上的寄生虫的方法。用于检测异物的方法包括检测通过用可见光照射诸如寄生虫的异物而激发的荧光。该方法适用于检测肉类寄生虫。用于检测寄生虫的方法包括用不传播激发光的滤光器选择性地检测从寄生虫发射的荧光，但是传播从寄生虫发射的荧光。用于检测寄生虫的装置包括用具有预定波长的可见光照射样本的光源，吸收激发光的漫反射的滤光器，从样本发出荧光的照相机，图像处理单元和监视器。

7. 发明申请

US20100239473A1 APPARATUS FOR DECOMPOSING ORGANIC MATTER WITH RADICAL TREATMENT METHOD USING ELECTRIC DISCHARGE 审中-公开
标题翻译：使用放电的放射治疗方法分解有机物的装置
公开(公告)号：US20100239473A1
公开(公告)日：2010-09-23
申请号：US12790134
申请日：2010-05-28
申请人： Takanori IIJIMA , Takaaki Murata , Kie Kubo , Yuji Okita , Shinji Kobayashi , Toru Ebata
发明人： Takanori IIJIMA , Takaaki Murata , Kie Kubo , Yuji Okita , Shinji Kobayashi , Toru Ebata
IPC分类号： B01J19/12
CPC分类号： C02F1/4608 , B01J19/088 , B01J2219/0813 , B01J2219/0828 , B01J2219/083 , B01J2219/0849 , B01J2219/0875 , C01B13/11 , C01B2201/14 , C01B2201/32 , C01B2201/64 , C01B2201/82 , C02F1/4672 , C02F1/74 , C02F2001/46152 , C02F2101/305 , C02F2101/366 , C02F2201/46175 , C02F2201/4618 , C02F2201/4619 , C02F2305/023
摘要： An apparatus performs radical treatment by electric discharge. The radical treatment apparatus includes an electrode unit having a gas flow path that blows gas onto treatment water and an electrode member that generates the electric discharge at a leading edge in order to generate a radical from the gas.
摘要翻译：一种装置通过放电进行激进治疗。自由基处理装置包括具有将气体吹向处理水的气体流路的电极单元和在前端产生放电的电极构件，以从气体产生自由基。

8. 发明申请

US20050189278A1 Apparatus for decomposing organic matter with radical treatment method using electric discharge 审中-公开
标题翻译：用放电自由基处理方法分解有机物的装置
公开(公告)号：US20050189278A1
公开(公告)日：2005-09-01
申请号：US11047761
申请日：2005-02-02
申请人： Takanori Iijima , Takaaki Murata , Kie Kubo , Yuji Okita , Shinji Kobayashi , Toru Ebata
发明人： Takanori Iijima , Takaaki Murata , Kie Kubo , Yuji Okita , Shinji Kobayashi , Toru Ebata
IPC分类号： B01J19/08 , C01B13/11 , C02F1/46 , C02F1/461 , C02F1/467 , C02F1/74 , C02F1/78
CPC分类号： C02F1/4608 , B01J19/088 , B01J2219/0813 , B01J2219/0828 , B01J2219/083 , B01J2219/0849 , B01J2219/0875 , C01B13/11 , C01B2201/14 , C01B2201/32 , C01B2201/64 , C01B2201/82 , C02F1/4672 , C02F1/74 , C02F2001/46152 , C02F2101/305 , C02F2101/366 , C02F2201/46175 , C02F2201/4618 , C02F2201/4619 , C02F2305/023
摘要： An apparatus performs radical treatment by electric discharge. The radical treatment apparatus includes an electrode unit having a gas flow path that blows gas onto treatment water and an electrode member that generates the electric discharge at a leading edge in order to generate a radical from the gas.
摘要翻译：一种装置通过放电进行激进治疗。自由基处理装置包括具有将气体吹向处理水的气体流路的电极单元和在前端产生放电的电极构件，以从气体产生自由基。

9. 发明申请

US20050106311A1 Aqueous extract of rice bran and utilization thereof in additives for ground fish meat products 失效
标题翻译：米糠水提取物及其在地面鱼肉制品添加剂中的应用
公开(公告)号：US20050106311A1
公开(公告)日：2005-05-19
申请号：US10499302
申请日：2002-12-10
申请人： Yuji Okita , Masayuki Nasu , Junko Doshida , Ikuo Kimura
发明人： Yuji Okita , Masayuki Nasu , Junko Doshida , Ikuo Kimura
IPC分类号： A23L1/10 , A23L1/30 , A23L1/325 , A23L1/28
CPC分类号： A23L17/70 , A23L7/115 , A23L17/00 , A23L33/105 , A23V2002/00 , A23V2250/21 , A23V2200/20
摘要： The components which have suwari-inhibiting action of rice bran extracts is reduced or removed, enabling broader utilization of them for fish paste products. A rice bran extract in which components having specific physiologically active functions are singled out, and these components are reduced or removed. The components are suwari-inhibiting components. The rice bran extract is preferably a water extract. The components are reduced or removed by precipitation or dialysis. The precipitation is calcium precipitation. The suwari-inhibiting components are phytin and/or phytic acid. The rice bran extract can be used as a fish paste additive.
摘要翻译：减少或去除了米糠提取物的suwari抑制作用的成分，可以更广泛地利用它们用于鱼酱产品。选择具有特定生理活性功能的成分的米糠提取物，并将这些成分还原或除去。组件是suwari抑制组件。米糠提取物优选为水提取物。通过沉淀或透析减少或去除组分。沉淀是钙沉淀。 suwari抑制成分是植酸和/或植酸。米糠提取物可用作鱼糊添加剂。

10. 发明授权

US4228476A Protective relaying system 失效
标题翻译：保护继电系统
公开(公告)号：US4228476A
公开(公告)日：1980-10-14
申请号：US915231
申请日：1978-06-13
申请人： Yuji Okita , Ryotaro Kondo , Yoshiji Nii
发明人： Yuji Okita , Ryotaro Kondo , Yoshiji Nii
IPC分类号： H02H3/28 , H02H1/00 , H02H3/05 , H02H3/30 , H02H7/26
CPC分类号： H02H3/05 , H02H1/0061 , H02H3/30
摘要： In a protective system which operates in response to a plurality of state quantities, for example voltage and current, one of which being a digital code signal having a long code length and transmitted over a transmission circuit, the density of the code errors of the digital code signal is detected. A plurality of state quantities are processed by a processor including first means responsive to the digital code signal and second means not responsive to the digital code signal. Either one of the first and second means is selected according to the density of the code errors.
摘要翻译：在响应于多个状态量（例如电压和电流）操作的保护系统中，其中一个是具有长码长度并通过传输电路传输的数字码信号，数字的码错误的密度检测码信号。多个状态量由处理器处理，该处理器包括响应于数字代码信号的第一装置和不响应数字代码信号的第二装置。根据码错误的密度选择第一和第二装置中的任何一个。

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