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    • 10. 发明申请
    • Methods and Apparatuses for Chip-Based DNA Error Reduction
    • 基于芯片的DNA错误减少的方法和设备
    • US20120028843A1
    • 2012-02-02
    • US13164045
    • 2011-06-20
    • Senthil RamuJoseph Jacobson
    • Senthil RamuJoseph Jacobson
    • C40B50/06C07H21/00C12P19/34
    • C12N15/1058C12Q1/6848C12Q2521/514C12Q2565/537
    • Methods and apparatus relate to reduction of sequence errors generated during synthesis of nucleic acids on a microarray chip. The error reduction can include synthesis of complementary stands (to template strands), using a short universal primer complementary to the template strands and polymerase. Heteroduplex can be formed be melting and re-annealing complementary stands and template strands. The heteroduplexes containing a mismatch can be recognized and cleaved by a mismatch endonuclease. The mismatch-containing cleaved heteroduplexes can be removed from the microarray chip using a global buffer exchange. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.
    • 方法和装置涉及减少在微阵列芯片上的核酸合成期间产生的序列错误。 错误减少可以包括使用与模板链和聚合酶互补的短通用引物来合成互补基团(模板链)。 Heteroduplex可以形成为熔化和重新退火互补架和模板链。 含有不匹配的异源双链可以被不匹配的内切核酸酶识别和切割。 可以使用全局缓冲液交换从微阵列芯片去除包含错配的切割的异源双链体。 由此产生的无错误的合成核酸可用于各种应用,包括合成生物燃料和增值药物产品。