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1. 发明申请

US20050208620A1 Compositions and methods for hydroxylating epothilones 有权
公开(公告)号：US20050208620A1
公开(公告)日：2005-09-22
申请号：US10915172
申请日：2004-08-09
申请人： Jonathan Basch , Shu-Jen Chiang , Suo-Win Liu , Akbar Nayeem , Yuhua Sun , Li You
发明人： Jonathan Basch , Shu-Jen Chiang , Suo-Win Liu , Akbar Nayeem , Yuhua Sun , Li You
IPC分类号： C07K14/79 , C12N9/02 , C12P17/18 , C07H21/04 , C12N1/21 , C12N9/06 , C12N15/74
CPC分类号： C12N9/0077 , C07K14/79 , C07K2299/00 , C12P17/181
摘要： Isolated nucleic acid sequences and polypeptides encoded thereby for epothilone B hydroxylase and mutants and variants thereof and a ferredoxin located downstream from the epothilone B hydroxylase gene are provided. Also provided are vectors and cells containing these vectors. In addition, methods for producing recombinant microorganisms, methods for using these recombinant microorganism to produce hydroxyalkyl-bearing epothilones and an epothilone analog produced by a mutant of epothilone B hydroxylase are provided.

2. 发明授权

US08158394B2 Genetically stable plasmid expressing PDH and FDH enzymes 有权
标题翻译：遗传稳定的表达PDH和FDH酶的质粒
公开(公告)号：US08158394B2
公开(公告)日：2012-04-17
申请号：US12756700
申请日：2010-04-08
申请人： Jonathan Basch , Thomas Franceschini , Suo Win Liu , Shu-Jen Chiang
发明人： Jonathan Basch , Thomas Franceschini , Suo Win Liu , Shu-Jen Chiang
IPC分类号： C12P17/06 , C12N9/02 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/0008 , C12N9/0018
摘要： Bi-cistronic plasmids used for the expression of formate dehydrogenase (FDH) and modified phenylalanine dehydrogenase (PDHmod) are provided.
摘要翻译：提供了用于表达甲酸脱氢酶（FDH）和改性苯丙氨酸脱氢酶（PDHmod）的双顺反子质粒。

3. 发明申请

US20100261236A1 Genetically Stable Plasmid Expressing PDH and FDH Enzymes 有权
标题翻译：表达PDH和FDH酶的基因稳定质粒
公开(公告)号：US20100261236A1
公开(公告)日：2010-10-14
申请号：US12756700
申请日：2010-04-08
申请人： Jonathan Basch , Thomas Franceschini , Suo Win Liu , Shu-Jen Chiang
发明人： Jonathan Basch , Thomas Franceschini , Suo Win Liu , Shu-Jen Chiang
IPC分类号： C12P7/62 , C07H21/04 , C12N15/63 , C12N1/21
CPC分类号： C12N9/0008 , C12N9/0018
摘要： Bi-cistronic plasmids used for the expression of formate dehydrogenase (FDH) and modified phenylalanine dehydrogenase (PDHmod) are provided.
摘要翻译：提供了用于表达甲酸脱氢酶（FDH）和改性苯丙氨酸脱氢酶（PDHmod）的双顺反子质粒。

4. 发明授权

US08119389B2 Rhodococcus erythropolis strain 有权
标题翻译：红球菌红细菌菌株
公开(公告)号：US08119389B2
公开(公告)日：2012-02-21
申请号：US12506596
申请日：2009-07-21
申请人： Nigel Ian Bowers , Paul M. Skonezny , Gregory L. Stein , Thomas Franceschini , Shu-Jen Chiang , Wendy L. Anderson , Li You , Zizhuo Xing
发明人： Nigel Ian Bowers , Paul M. Skonezny , Gregory L. Stein , Thomas Franceschini , Shu-Jen Chiang , Wendy L. Anderson , Li You , Zizhuo Xing
IPC分类号： C12N1/20
CPC分类号： C12R1/01 , C12P13/001
摘要： The present invention relates to a process for preparing (2R,3S)-1-halo-2-hydroxy-3-(protected)amino-4-substituted butane comprising contacting a mutagenized Rhodococcus erythropolis having ATCC deposit no. PTA-6648 with (3S)-1-halo-2-oxo-3-(protected)amino-4-substituted butane substrate. The present invention also relates to a process comprising mixing (2R,3S)-1-halo-2-hydroxy-3-(protected)amino-4-substituted butane with at least one base in the presence of at least one solvent to produce a reaction mixture containing (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane. The present invention further relates to a process comprising crystallizing the (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane out of the reaction mixture by concurrently adding water and the reaction mixture together. The present invention is also directed to a mutagenized Rhodococcus erythropolis having ATCC deposit no. PTA-6648.
摘要翻译：本发明涉及制备（2R，3S）-1-卤代-2-羟基-3-（被保护的）氨基-4-取代的丁烷的方法，包括将具有ATCC保藏号的诱变的红球菌红景天（3S）-1-卤代-2-氧代-3-（保护的）氨基-4-取代的丁烷底物的PTA-6648。本发明还涉及一种方法，其包括在至少一种溶剂的存在下将（2R，3S）-1-卤代-2-羟基-3-（保护的）氨基-4-取代的丁烷与至少一个碱混合以产生含有（2R，3S）-1,2-环氧-3-（保护）氨基-4-取代丁烷的反应混合物。本发明还涉及通过将水和反应混合物同时加入，使（2R，3S）-1,2-环氧-3-（被保护的）氨基-4-取代丁烷从反应混合物中结晶出来的方法。本发明还涉及具有ATCC保藏号的诱变的红球菌红球菌。 PTA-6648。

5. 发明授权

US07582468B2 Process for preparing (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane and intermediates thereof 有权
标题翻译：制备（2R，3S）-1,2-环氧-3-（保护）氨基-4-取代丁烷的方法及其中间体
公开(公告)号：US07582468B2
公开(公告)日：2009-09-01
申请号：US11365275
申请日：2006-03-01
申请人： Nigel Ian Bowers , Paul M. Skonezny , Gregory L. Stein , Thomas Franceschini , Shu-Jen Chiang , Wendy L. Anderson , Li You , Zizhuo Xing
发明人： Nigel Ian Bowers , Paul M. Skonezny , Gregory L. Stein , Thomas Franceschini , Shu-Jen Chiang , Wendy L. Anderson , Li You , Zizhuo Xing
IPC分类号： C12P41/00
CPC分类号： C12R1/01 , C12P13/001
摘要： The present invention relates to a process for preparing (2R,3S)-1-halo-2-hydroxy-3-(protected)amino-4-substituted butane comprising contacting a mutagenized Rhodococcus erythropolis having ATCC deposit no. PTA-6648 with (3S)-1-halo-2-oxo-3-(protected)amino-4-substituted butane substrate. The present invention also relates to a process comprising mixing (2R,3S)-1-halo-2-hydroxy-3-(protected)amino-4-substituted butane with at least one base in the presence of at least one solvent to produce a reaction mixture containing (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane. The present invention further relates to a process comprising crystallizing the (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane out of the reaction mixture by concurrently adding water and the reaction mixture together. The present invention is also directed to a mutagenized Rhodococcus erythropolis having ATCC deposit no. PTA-6648.
摘要翻译：本发明涉及制备（2R，3S）-1-卤代-2-羟基-3-（被保护的）氨基-4-取代的丁烷的方法，包括将具有ATCC保藏号的诱变的红球菌红景天（3S）-1-卤代-2-氧代-3-（保护的）氨基-4-取代的丁烷底物的PTA-6648。本发明还涉及一种方法，其包括在至少一种溶剂的存在下将（2R，3S）-1-卤代-2-羟基-3-（保护的）氨基-4-取代的丁烷与至少一个碱混合以产生含有（2R，3S）-1,2-环氧-3-（保护）氨基-4-取代丁烷的反应混合物。本发明还涉及通过将水和反应混合物同时加入，使（2R，3S）-1,2-环氧-3-（被保护的）氨基-4-取代丁烷从反应混合物中结晶出来的方法。本发明还涉及具有ATCC保藏号的诱变的红球菌红球菌。 PTA-6648。

6. 发明申请

US20090286303A1 PROCESS FOR PREPARING (2R,3S)-1,2-EPOXY-3-(PROTECTED) AMINO-4-SUBSTITUTED BUTANE AND INTERMEDIATES THEREOF 有权
标题翻译：制备（2R，3S）-1,2-环氧-3-（保护的）氨基-4-取代的丁烷的方法及其中间体
公开(公告)号：US20090286303A1
公开(公告)日：2009-11-19
申请号：US12506596
申请日：2009-07-21
申请人： Nigel Ian Bowers , Paul M. Skonezny , Gregory L. Stein , Thomas Franceschini , Shu-Jen Chiang , Wendy L. Anderson , Li You , Zizhuo Xing
发明人： Nigel Ian Bowers , Paul M. Skonezny , Gregory L. Stein , Thomas Franceschini , Shu-Jen Chiang , Wendy L. Anderson , Li You , Zizhuo Xing
IPC分类号： C12N1/21
CPC分类号： C12R1/01 , C12P13/001
摘要： The present invention relates to a process for preparing (2R,3S)-1-halo-2-hydroxy-3-(protected)amino-4-substituted butane comprising contacting a mutagenized Rhodococcus erythropolis having ATCC deposit no. PTA-6648 with (3S)-1-halo-2-oxo-3-(protected)amino-4-substituted butane substrate. The present invention also relates to a process comprising mixing (2R,3S)-1-halo-2-hydroxy-3-(protected)amino-4-substituted butane with at least one base in the presence of at least one solvent to produce a reaction mixture containing (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane. The present invention further relates to a process comprising crystallizing the (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane out of the reaction mixture by concurrently adding water and the reaction mixture together. The present invention is also directed to a mutagenized Rhodococcus erythropolis having ATCC deposit no. PTA-6648.
摘要翻译：本发明涉及制备（2R，3S）-1-卤代-2-羟基-3-（被保护的）氨基-4-取代的丁烷的方法，其包括将具有ATCC保藏号的诱变的红球菌红景天（3S）-1-卤代-2-氧代-3-（保护的）氨基-4-取代的丁烷底物的PTA-6648。本发明还涉及一种方法，其包括在至少一种溶剂的存在下将（2R，3S）-1-卤代-2-羟基-3-（保护的）氨基-4-取代的丁烷与至少一个碱混合以产生含有（2R，3S）-1,2-环氧-3-（保护）氨基-4-取代丁烷的反应混合物。本发明还涉及通过将水和反应混合物同时加入，使（2R，3S）-1,2-环氧-3-（被保护的）氨基-4-取代丁烷从反应混合物中结晶出来的方法。本发明还涉及具有ATCC保藏号的诱变的红球菌红球菌。 PTA-6648。

7. 发明申请

US20060270012A1 Process for preparing (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane and intermediates thereof 有权
公开(公告)号：US20060270012A1
公开(公告)日：2006-11-30
申请号：US11365275
申请日：2006-03-01
申请人： Nigel Bowers , Paul Skonezny , Gregory Stein , Thomas Franceschini , Shu-Jen Chiang , Wendy Anderson , Li You , Zizhou Xing
发明人： Nigel Bowers , Paul Skonezny , Gregory Stein , Thomas Franceschini , Shu-Jen Chiang , Wendy Anderson , Li You , Zizhou Xing
IPC分类号： C12P7/02
CPC分类号： C12R1/01 , C12P13/001
摘要： The present invention relates to a process for preparing (2R,3S)-1-halo-2-hydroxy-3-(protected)amino-4-substituted butane comprising contacting a mutagenized Rhodococcus erythropolis having ATCC deposit no. PTA-6648 with (3S)-1-halo-2-oxo-3-(protected)amino-4-substituted butane substrate. The present invention also relates to a process comprising mixing (2R,3S)-1-halo-2-hydroxy-3-(protected)amino-4-substituted butane with at least one base in the presence of at least one solvent to produce a reaction mixture containing (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane. The present invention further relates to a process comprising crystallizing the (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane out of the reaction mixture by concurrently adding water and the reaction mixture together. The present invention is also directed to a mutagenized Rhodococcus erythropolis having ATCC deposit no. PTA-6648.

8. 发明申请

US20070122891A1 Methods for the Preparation, Isolation and Purification of Epothilone B, and X-Ray Crystal Structures of Epothilone B 有权
标题翻译：埃坡霉素B的制备，分离和纯化方法以及埃坡霉素B的X射线晶体结构
公开(公告)号：US20070122891A1
公开(公告)日：2007-05-31
申请号：US11669268
申请日：2007-01-31
申请人： Daniel Benigni , Robert Stankavage , Shu-Jen Chiang , Hsing Hou , Bruce Eagan , Dennis Gu , David Hou , Les Mintzmyer , Thomas Tully , Brian Davis , Ivan Hargro , Mark Mascari , Gabriel Galvin , Gregory Stein , Cary McConlogue , Fahri Comezoglu
发明人： Daniel Benigni , Robert Stankavage , Shu-Jen Chiang , Hsing Hou , Bruce Eagan , Dennis Gu , David Hou , Les Mintzmyer , Thomas Tully , Brian Davis , Ivan Hargro , Mark Mascari , Gabriel Galvin , Gregory Stein , Cary McConlogue , Fahri Comezoglu
IPC分类号： C12P17/00 , C12N1/20 , C07D417/02
CPC分类号： C07D493/04 , C12N1/20 , C12N1/38 , C12P17/181 , C12R1/01
摘要： The present invention relates to improved methods for the production, isolation and purification of epothilone B. These methods include, for example, a fermentation process for the production of epothilone B, isolation via adsorption onto a resin, and subsequent purification.
摘要翻译：本发明涉及埃坡霉素B的生产，分离和纯化的改进方法。这些方法包括例如用于生产埃坡霉素B的发酵方法，通过吸附到树脂上进行分离和随后的纯化。

9. 发明授权

US5516679A Penicillin V amidohydrolase gene from Fusarium oxysporum 失效
标题翻译：来自尖孢镰刀菌的青霉素V酰氨基水解酶基因
公开(公告)号：US5516679A
公开(公告)日：1996-05-14
申请号：US363475
申请日：1994-12-23
申请人： Shu-Jen Chiang , William V. Burnett, Jr. , Sean M. Tonzi
发明人： Shu-Jen Chiang , William V. Burnett, Jr. , Sean M. Tonzi
IPC分类号： C12N1/15 , C07H21/04 , C07K1/22 , C07K14/195 , C07K14/37 , C07K14/41 , C12N1/21 , C12N9/06 , C12N9/84 , C12N9/86 , C12N15/09 , C12N15/11 , C12N15/55 , C12N15/63 , C12N15/80 , C12P21/02 , C12R1/19 , C12R1/77 , C12N15/70
CPC分类号： C12Y104/03003 , C12N15/80 , C12N9/0024 , C12N9/84 , C12Y305/01011
摘要： Nucleic acids coding for penicillin V amidohydrolase (PVA) from Fusarium oxysporum. Also, expression vectors, host cells and a method for production of PVA.
摘要翻译：从尖孢镰刀菌编码青霉素V酰氨基水解酶（PVA）的核酸。此外，表达载体，宿主细胞和用于生产PVA的方法。

10. 发明授权

US07172884B2 Methods for the preparation, isolation and purification of epothilone B, and x-ray crystal structures of epothilone B 有权
标题翻译：埃坡霉素B的制备，分离和纯化方法以及埃坡霉素B的X射线晶体结构
公开(公告)号：US07172884B2
公开(公告)日：2007-02-06
申请号：US10668032
申请日：2003-09-22
申请人： Daniel Benigni , Robert Stankavage , Shu-Jen Chiang , Hsing Hou , Bruce Eagan , Dennis Gu , David Hou , Les Mintzmyer , Thomas P. Tully , Brian L. Davis , Ivan Hargro , Mark Mascari , Gabriel Galvin , Gregory Stein , Cary W. McConlogue , Fahri T. Comezoglu
发明人： Daniel Benigni , Robert Stankavage , Shu-Jen Chiang , Hsing Hou , Bruce Eagan , Dennis Gu , David Hou , Les Mintzmyer , Thomas P. Tully , Brian L. Davis , Ivan Hargro , Mark Mascari , Gabriel Galvin , Gregory Stein , Cary W. McConlogue , Fahri T. Comezoglu
IPC分类号： C08B30/18 , A61K31/724 , A61K31/715 , C12P17/18
CPC分类号： C07D493/04 , C12N1/20 , C12N1/38 , C12P17/181 , C12R1/01
摘要： The present invention relates to improved methods for the production, isolation and purification of epothilone B. These methods include, for example, a fermentation process for the production of epothilone B, isolation via adsorption onto a resin, and subsequent purification.
摘要翻译：本发明涉及埃坡霉素B的生产，分离和纯化的改进方法。这些方法包括例如用于生产埃坡霉素B的发酵方法，通过吸附到树脂上进行分离和随后的纯化。

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