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1. 发明授权

US5028541A Flow-through cell cultivation system 失效
标题翻译：流通细胞培养系统
公开(公告)号：US5028541A
公开(公告)日：1991-07-02
申请号：US56034
申请日：1987-06-01
申请人： Johnathan L. Kiel , David N. Erwin , David M. Simmons
发明人： Johnathan L. Kiel , David N. Erwin , David M. Simmons
IPC分类号： C12M3/00
CPC分类号： C12M35/02 , C12M23/42 , C12M29/10 , C12M41/12
摘要： A flow-through cell cultivation apparatus and method is described. The flow-through cell cultivation system comprises a flowcell, a chamber inside the flowcell for holding cells, and a flowcell holder. The flowcell has a lower intake port for flowing liquid nutrient through a cellbed inside the flowcell and an upper outlet port for flowing the liquid nutrient out of the flowcell. The flowcell also includes a chuck for receipt of a thermal probe. The probe is made of electromagnetically non-interactive material. The flowcell is enclosed inside the flowcell holder. The flowcell holder includes a pair of intake ports into a cavity having an open end at the flowcell for turbulently flowing a temperature controlled gas against the flowcell. The cavity has rough walls to promote the turbulent flow. The flowcell holder includes an exhaust port for flowing the gas out of the flowcell holder which also serves as a port for another thermal probe. A one-way valve prevents flow of the liquid nutrient out of the chamber through the intake port. Temperature controlled air is supplied to the flowcell holder by dividing air from an air supply into two conduits. One conduit travels through a coil inside a refrigerated liquid bath and the other through a coil inside a heated liquid bath. The heated and cooled air are recombined to make an even temperature air supply which is then divided once more to provide the two air supplies for creating a turbulent supply of temperature controlled air through the two flowcell holder intake ports. The flowcell may include on one side a semi-permeable membrane for introducing a gas/liquid interface to the cellbed.
摘要翻译：描述了流通细胞培养装置和方法。流通池培养系统包括流通池，用于保持电池的流通池内的室和流通池保持器。流通池具有用于使液体营养物流过流动池内的池底的较低进气口和用于使液体营养物流出流动池的上部出口。流通池还包括用于接收热探针的卡盘。探头由电磁非交互材料制成。流通池封闭在流通池支架内。流通池保持器包括一对进入端口，其具有在流通池处具有开口端的空腔，用于将受温度控制的气体湍流地流向流动池。空腔具有粗糙的壁，以促进湍流。流通池保持器包括用于将气体从流动池保持器流出的排气口，其还用作另一热探针的端口。单向阀防止液体营养物质通过进气口流出室外。通过将空气从空气供应分成两个管道，将温度控制的空气供应到流通池保持器。一个导管穿过冷藏液槽内的线圈，另一个通过加热液槽内的线圈。加热和冷却的空气被重新组合以产生均匀的温度空气供应，然后再分配一次，以提供两个空气供应，以通过两个流通池保持器进气口产生温度受控空气的湍流供应。流动池可以在一侧包括用于将气体/液体界面引入细胞床的半透膜。

2. 发明授权

US09273345B2 Methods and compositions for processes of rapid selection and production of nucleic acid aptamers 有权
标题翻译：快速选择和生产核酸适体的方法和组合物
公开(公告)号：US09273345B2
公开(公告)日：2016-03-01
申请号：US11965039
申请日：2007-12-27
申请人： Johnathan L. Kiel , Eric A. Holwitt , Michael (Maomian) Fan , Shelly D. Roper
发明人： Johnathan L. Kiel , Eric A. Holwitt , Michael (Maomian) Fan , Shelly D. Roper
IPC分类号： C12N15/115 , C12Q1/68
CPC分类号： C12Q1/6811 , C12N15/115 , Y10T436/143333 , C12Q2525/205 , C12Q2565/101
摘要： Embodiments herein relate to compositions and methods for making and using aptamers, for example, DNA aptamers (DCEs) and/or RNA aptamers. In some embodiments, methods relate to making and amplifying target DCEs. In certain embodiments, methods for making capture elements or aptamers concern using a reporter moiety and signal reducing moiety prior to amplifying a target-specific capture element. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of aptamers such as DCEs directed to a particular target agent. Some embodiments relate to systems for performing automated generation of aptamers.
摘要翻译：本文的实施方案涉及用于制备和使用适体的组合物和方法，例如DNA适体（DCE）和/或RNA适体。在一些实施方案中，方法涉及制备和扩增目标DCE。在某些实施方案中，用于制备捕获元件或适体的方法在放大靶特异性捕获元件之前关注使用报道部分和信号降低部分。在一些实施方案中，本文公开的方法可用于快速产生大量适配体，例如针对特定目标试剂的DCE。一些实施例涉及用于执行适配体的自动生成的系统。

3. 发明授权

US08092988B1 Medium and method of deriving and using a mutated bacteria strain 有权
标题翻译：培养基和衍生和使用突变菌株的方法
公开(公告)号：US08092988B1
公开(公告)日：2012-01-10
申请号：US11820039
申请日：2007-05-25
申请人： Jill E. Parker , Johnathan L. Kiel , Homer Gifford , John L. Alls , Pedro J. Morales, legal representative
发明人： Jill E. Parker , Johnathan L. Kiel , Homer Gifford , John L. Alls
IPC分类号： C12Q1/00 , C12N15/00
CPC分类号： C12R1/07 , A61K39/07 , A61K2039/522
摘要： A new strain of Bacillus anthracis derived from the Sterne vaccine strain of Bacillus anthracis by growth on a high-nitrate-concentration, 3-amino-L-tyrosine growth medium.
摘要翻译：通过在高硝酸盐浓度的3-氨基-L-酪氨酸生长培养基上生长，衍生自炭疽芽孢杆菌的Sterne疫苗株的新型芽孢杆菌菌株。

4. 发明授权

US5856108A Biosynthesis of diazomelanin and diazoluminomelanin and methods thereof 失效
标题翻译：重氮木兰素和二叠氮木兰素的生物合成及其方法
公开(公告)号：US5856108A
公开(公告)日：1999-01-05
申请号：US779694
申请日：1991-10-21
申请人： Johnathan L. Kiel , Jill E. Parker , Eric A. Holwitt , Harvey A. Schwertner
发明人： Johnathan L. Kiel , Jill E. Parker , Eric A. Holwitt , Harvey A. Schwertner
IPC分类号： C12N9/02 , C12P17/12 , C12Q1/12 , G01N33/58 , C12Q1/00 , C12Q1/04 , C12Q1/26
CPC分类号： C12N9/0036 , C12P17/12 , C12Q1/12 , G01N33/582
摘要： There is provided a method for producing diazoluminomelanin (DALM) which comprises culturing in a medium containing nitrate, 3-amino-L-tyrosine (3-AT) and luminol under suitable metabolic conditions, a microorganism containing nitrate reductase.Also provided is a method for directly detecting microorganisms containing nitrate reductase or those into which nitrate reductase can be introduced by recombinant DNA technology, which comprises culturing the microorganism in a medium containing nitrate, 3-amino-L-tyrosine (3-AT) and luminol under suitable metabolic conditions, transferring the medium to a microtiter plate or tube coated with antibody or an antiligand to which the microorganism would specifically bind, washing the plate or tube and activating luminescence.Further, there is provided a method for producing diazomelanin (DM) which comprises culturing in a medium containing nitrate and 3-amino-L-tyrosine (3-AT) under suitable metabolic conditions, a microorganism containing nitrate reductase.Yet further, there is provided a method for directly detecting microorganisms containing nitrate reductase or those into which nitrate reductase can be introduced by recombinant DNA technology, which comprises culturing the microorganism in a medium containing nitrate and 3-amino-L-tyrosine (3-AT) under suitable metabolic conditions, transferring the medium to a microtiter plate or tube coated with antibody or an antiligand to which the microorganism would specifically bind, washing the plate or tube, adding luminol and activating luminescence.
摘要翻译：提供了一种生产重氮酚蓝蛋白（DALM）的方法，该方法包括在合适的代谢条件下，在含硝酸盐，3-氨基-L-酪氨酸（3-AT）和鲁米诺的培养基中培养含有硝酸还原酶的微生物。还提供了一种用于直接检测含有硝酸还原酶的微生物的方法或其中可以通过重组DNA技术引入硝酸还原酶的方法，其包括在含有硝酸盐，3-氨基-L-酪氨酸（3-AT）和将鲁米诺转移到适当的代谢条件下，将培养基转移到用抗体或微生物将特异性结合的抗配体的微量滴定板或管，洗涤板或管并激活发光。此外，提供了一种生产重氮木兰素（DM）的方法，其包括在适当的代谢条件下在含有硝酸盐和3-氨基-L-酪氨酸（3-AT）的培养基中培养含有硝酸还原酶的微生物。此外，提供了一种直接检测含有硝酸还原酶的微生物的方法或其中可以通过重组DNA技术引入硝酸还原酶的方法，其包括在含有硝酸盐和3-氨基-L-酪氨酸（3- AT），将培养基转移到用抗体或微生物将特异性结合的抗配体的微量滴定板或管，洗涤板或管，加入鲁米诺并激活发光。

5. 发明授权

US4870002A Method of prevention of oxidative injury to cells 失效
标题翻译：预防细胞氧化损伤的方法
公开(公告)号：US4870002A
公开(公告)日：1989-09-26
申请号：US804819
申请日：1985-12-04
申请人： Johnathan L. Kiel
发明人： Johnathan L. Kiel
IPC分类号： A61K38/44 , C12N11/18
CPC分类号： C12N11/18 , A61K31/198
摘要： A process for preventing oxidative injury in which living cells are contacted with a protective amount of 3-amino-tyrosine or a derivative thereof. Also provided is a process for preserving a cell sample involving collecting a sample of cells and adding 3-aminotyrosine or a derivative thereof to that cell sample. A process for preventing thermal damage to living cells is also provided which comprises contacting the cells with a thermal protectant amount of 3-aminotyrosine or a derivative thereof, as is a process for protecting living cells from damage caused by ionizing radiation, which comprises contacting the cells with a radioprotectant amount of 3-aminotyrosine or a derivative thereof. Also provided is a process for suppressing anti-inflammatory reactions in animals which comprises administering an anti-inflammatory amount of 3-aminotrosine or a derivative thereof to an animal.
摘要翻译：一种预防氧化损伤的方法，其中活细胞与保护量的3-氨基 - 酪氨酸或其衍生物接触。还提供了一种保存细胞样品的方法，其涉及收集细胞样品并向该细胞样品中加入3-氨基酪氨酸或其衍生物。还提供了防止活细胞热损伤的方法，其包括使细胞与热保护剂量的3-氨基酪氨酸或其衍生物接触，保护活细胞免受电离辐射引起的损伤的方法，该方法包括使具有放射防护剂量的3-氨基酪氨酸或其衍生物的细胞。还提供了抑制动物抗炎反应的方法，其包括向动物施用抗炎量的3-氨基酪氨酸或其衍生物。

6. 发明授权

US4486408A Insoluble crosslinked cytotoxic oxidase-peroxidase system 失效
标题翻译：不溶性交联的细胞毒性氧化酶 - 过氧化物酶系统
公开(公告)号：US4486408A
公开(公告)日：1984-12-04
申请号：US251694
申请日：1981-04-07
申请人： Johnathan L. Kiel
发明人： Johnathan L. Kiel
IPC分类号： C12N1/06 , A61K38/44 , C12N11/00 , C12N11/18 , A61K37/48
CPC分类号： A61K38/44 , A61K38/385 , A61K38/443 , A61K47/42 , A61K9/0019 , A61K9/14 , A61K9/146 , A61K9/19 , C12N11/18 , C12Y101/03004 , C12Y111/01007 , A61K9/06
摘要： A therapeutic preparation, and a process of using the preparation, for the control of tumor growth and immune diseases. The preparation is an immobilized cytotoxic enzyme system which is carried by an insoluble substrate that is administered into the mammalian subject by injection or implantation. The preferred embodiment of the preparation uses a polymerized albumin carrier containing immobilized glucose oxidase and peroxidase. The immobilized enzyme system is prepared in the form of small particles that can be administered either by implantation or by injection into tumor-bearing mammals. To date, antibacterial activity has been demonstrated only in vitro. Intraperitoneal injection into rats bearing Novikoff hepatomas or into mice bearing B16-F1 melanomas or L1210 (Lymphoid) tumors has resulted in the selected killing of tumor tissues. Several daily administrations are required to achieve complete destruction of the tumor. Additionally, the preparation is also active as a stimulant of the specific immune response to neoplasia. Its effect on the immune system could be useful in the stimulation of the immune system in diseases where the action of the endogenous immune system is insufficient.
摘要翻译：治疗制剂和使用该制剂的方法用于控制肿瘤生长和免疫疾病。该制剂是固定的细胞毒素酶系统，其由通过注射或植入施用于哺乳动物受试者的不溶性底物携带。制剂的优选实施方案使用含有固定化葡萄糖氧化酶和过氧化物酶的聚合的白蛋白载体。固定化酶系统以小颗粒的形式制备，可以通过植入或通过注射给含有哺乳动物的哺乳动物施用。迄今为止，抗菌活性仅在体外证明。腹腔注射带有Novikoff肝细胞的大鼠或携带B16-F1黑素瘤或L1210（淋巴样）肿瘤的小鼠导致选择性杀死肿瘤组织。需要几个日常管理来实现肿瘤的完全破坏。此外，该制剂也作为对瘤形成的特异性免疫应答的兴奋剂是有活性的。其对免疫系统的影响可用于在内源性免疫系统的作用不足的疾病中刺激免疫系统。

7. 发明授权

US08383036B2 Methods for neutralizing anthrax or anthrax spores 有权
标题翻译：中和炭疽芽孢或炭疽孢子的方法
公开(公告)号：US08383036B2
公开(公告)日：2013-02-26
申请号：US12973681
申请日：2010-12-20
申请人： Mark A. Sloan , Jeevalatha Vivekananda , Eric A. Holwitt , Johnathan L. Kiel
发明人： Mark A. Sloan , Jeevalatha Vivekananda , Eric A. Holwitt , Johnathan L. Kiel
IPC分类号： A61L2/00
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods, compositions and apparatus for neutralizing bioagents, wherein bioagents comprise biowarfare agents, biohazardous agents, biological agents and/or infectious agents. The methods comprise exposing the bioagent to an organic semiconductor and exposing the bioagent and organic semiconductor to a source of energy. Although any source of energy is contemplated, in some embodiments the energy comprises visible light, ultraviolet, infrared, radiofrequency, microwave, laser radiation, pulsed corona discharge or electron beam radiation. Exemplary organic semiconductors include DAT and DALM. In certain embodiments, the organic semiconductor may be attached to one or more binding moieties, such as an antibody, antibody fragment, or nucleic acid ligand. Preferably, the binding moiety has a binding affinity for one or more bioagents to be neutralized. Other embodiments concern an apparatus comprising an organic semiconductor and an energy source. In preferred embodiments, the methods, compositions and apparatus are used for neutralizing anthrax spores.
摘要翻译：本发明涉及用于中和生物标签的方法，组合物和装置，其中生物制剂包括生物武器，生物危害剂，生物制剂和/或感染剂。所述方法包括将生物制剂暴露于有机半导体并将生物制剂和有机半导体暴露于能量源。尽管预期了任何能量源，但在一些实施例中，能量包括可见光，紫外线，红外线，射频，微波，激光辐射，脉冲电晕放电或电子束辐射。示例性有机半导体包括DAT和DALM。在某些实施方案中，有机半导体可以连接到一个或多个结合部分，例如抗体，抗体片段或核酸配体。优选地，结合部分对一种或多种待中和的生物剂具有结合亲和力。其他实施例涉及包括有机半导体和能量源的装置。在优选的实施方案中，所述方法，组合物和装置用于中和炭疽芽孢。

8. 发明授权

US07892484B2 Methods and compositions for neutralizing anthrax and other bioagents 有权
标题翻译：用于中和炭疽和其他生物制剂的方法和组合物
公开(公告)号：US07892484B2
公开(公告)日：2011-02-22
申请号：US10291336
申请日：2002-11-08
申请人： Mark A. Sloan , Jeevalatha Vivekananda , Eric A. Holwitt , Johnathan L. Kiel
发明人： Mark A. Sloan , Jeevalatha Vivekananda , Eric A. Holwitt , Johnathan L. Kiel
IPC分类号： A61L2/00
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods, compositions and apparatus for neutralizing bioagents, wherein bioagents comprise biowarfare agents, biohazardous agents, biological agents and/or infectious agents. The methods comprise exposing the bioagent to an organic semiconductor and exposing the bioagent and organic semiconductor to a source of energy. Although any source of energy is contemplated, in some embodiments the energy comprises visible light, ultraviolet, infrared, radiofrequency, microwave, laser radiation, pulsed corona discharge or electron beam radiation. Exemplary organic semiconductors include DAT and DALM. In certain embodiments, the organic semiconductor may be attached to one or more binding moieties, such as an antibody, antibody fragment, or nucleic acid ligand. Preferably, the binding moiety has a binding affinity for one or more bioagents to be neutralized. Other embodiments concern an apparatus comprising an organic semiconductor and an energy source. In preferred embodiments, the methods, compositions and apparatus are used for neutralizing anthrax spores.
摘要翻译：本发明涉及用于中和生物标签的方法，组合物和装置，其中生物制剂包括生物武器，生物危害剂，生物制剂和/或感染剂。所述方法包括将生物制剂暴露于有机半导体并将生物制剂和有机半导体暴露于能量源。尽管预期了任何能量源，但在一些实施例中，能量包括可见光，紫外线，红外线，射频，微波，激光辐射，脉冲电晕放电或电子束辐射。示例性有机半导体包括DAT和DALM。在某些实施方案中，有机半导体可以连接到一个或多个结合部分，例如抗体，抗体片段或核酸配体。优选地，结合部分对一种或多种待中和的生物剂具有结合亲和力。其他实施例涉及包括有机半导体和能量源的装置。在优选的实施方案中，所述方法，组合物和装置用于中和炭疽芽孢。

9. 发明授权

US06878519B1 DNA probes for specific genes of anthrax 失效
标题翻译：针对炭疽特异性基因的DNA探针
公开(公告)号：US06878519B1
公开(公告)日：2005-04-12
申请号：US10339259
申请日：2003-01-06
申请人： Jill E. Parker , Johnathan L. Kiel
发明人： Jill E. Parker , Johnathan L. Kiel
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C07H21/04 , C12Q1/686 , C12Q1/689
摘要： A PCR-based method for the identification of Bacillus anthracis is described. The method utilizes novel primer sets; designated 2Xlg3F (SEQ ID NO 3), 2Xlg3R (SEQ ID NO 4), 2Xlg3F2 (SEQ ID NO 5), 2Xlg3R2 (SEQ ID NO 6), 4XH1a2F (SEQ ID NO 7), and 4XH1a2R (SEQ ID NO 8).
摘要翻译：描述了用于鉴定炭疽芽孢杆菌的基于PCR的方法。该方法利用新型引物组; 命名为2X1g3F（SEQ ID NO3），2X1G3R（SEQ ID NO4），2X1g3F2（SEQ ID NO：5），2X1g3R2（SEQ ID NO：6），4XH1a2F（SEQ ID NO：7）和4XH1a2R（SEQ ID NO：8）。

10. 发明授权

US5972721A Immunomagnetic assay system for clinical diagnosis and other purposes 失效
标题翻译：免疫磁性检测系统用于临床诊断等目的
公开(公告)号：US5972721A
公开(公告)日：1999-10-26
申请号：US816429
申请日：1997-03-14
申请人： John G. Bruno , Johnathan L. Kiel , John P. Kilian
发明人： John G. Bruno , Johnathan L. Kiel , John P. Kilian
IPC分类号： B03C1/01 , B03C1/032 , B03C1/033 , B03C1/034 , G01N33/533 , B03C1/00
CPC分类号： B03C1/032 , B03C1/01 , B03C1/0332 , B03C1/034 , Y10S436/824
摘要： An apparatus and method for immunomagnetic separation and concentration of target biological materials is disclosed. The immunomagnetic separation is performed by a magnetic flow cell, or filter block, as part of an automated mostly continuous immunomagnetic assay system. The magnetic flow cell has two bundles of ferromagnetic rods or pins positioned inside an internal chamber so that a fluid sample flowing through the flow cell passes through the pins. A pair of cobalt magnets flank the flow cell so that the pins concentrate and sufficiently increase the magnetic fields so that even nanometer size magnetic beads can be captured. The overall system combines a reaction subsystem for reacting coated magnetic beads with a sample, a collection subsystem for capturing magnetic beads, a rinsing subsystem for removing debris and a filtering subsystem for removing captured magnetic beads from the collection subsystem. The new magnetic flow filter is the key component for the collection and filtering subsystems.
摘要翻译：公开了一种用于免疫磁性分离和靶向生物材料浓缩的装置和方法。免疫磁性分离由磁流量池或过滤块进行，作为自动化大多数连续的免疫磁性测定系统的一部分。磁流量计具有两束位于内部室内的铁磁棒或销，使得流过流动池的流体样品通过销。在流动池侧面的一对钴磁体，使得引脚集中并充分增加磁场，使得可以捕获甚至纳米尺寸的磁珠。整个系统结合了用于使涂覆的磁珠与样品反应的反应子系统，用于捕获磁珠的收集子系统，用于去除碎屑的冲洗子系统和用于从收集子系统中去除捕获的磁珠的过滤子系统。新的磁流过滤器是收集和过滤子系统的关键组件。

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