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1. 发明申请

US20060166354A1 Bioreactors with multiple chambers 有权
标题翻译：具有多个室的生物反应器
公开(公告)号：US20060166354A1
公开(公告)日：2006-07-27
申请号：US10525549
申请日：2003-08-27
申请人： John Wikswo , Franz Baudenbacher , David Cliffel , Frederick Haselton , Eugene Leboeuf , Alex Prokop , Randall Reiserer , Mark Stremler
发明人： John Wikswo , Franz Baudenbacher , David Cliffel , Frederick Haselton , Eugene Leboeuf , Alex Prokop , Randall Reiserer , Mark Stremler
IPC分类号： C12M1/34 , C12M1/12
CPC分类号： C12M23/16 , B01L3/5027 , B01L2200/10 , B01L2300/0645 , B01L2300/0681 , B01L2300/0816 , B01L2300/163 , B01L2400/0472 , C12M23/12 , C12M29/10 , C12M41/00 , Y10S435/808
摘要： A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor has a first substrate having a first surface and an opposite second surface, defining a chamber therebetween for receiving the cells and the liquid medium. The bioreactor further has a barrier dividing the chamber into a first subchamber and a second subchamber, wherein the barrier has a porosity to allow the first subchamber and the second subchamber in fluid communication and allow at least one predetermined type of cells to permeate between the first subchamber and the second subchamber.
摘要翻译：用于在液体培养基中培养活细胞的生物反应器。在本发明的一个实施方案中，生物反应器具有第一基底，其具有第一表面和相对的第二表面，在其间限定用于接收细胞和液体介质的腔室。所述生物反应器还具有将所述室分成第一子室和第二子室的屏障，其中所述屏障具有孔隙度以允许所述第一子室和所述第二子室流体连通，并且允许至少一个预定类型的泡孔在所述第一室子房和第二个子房。

2. 发明申请

US20060141607A1 Capillary perfused bioreactors with multiple chambers 有权
公开(公告)号：US20060141607A1
公开(公告)日：2006-06-29
申请号：US10525559
申请日：2003-08-27
申请人： John Wikswo , Franz Baudenbacher , Alex Prokop , Eugene Leboeuf , Chang Chung , David Cliffel , Frederick Haselton , William Hofmeister , Charles Lin , Lisa McCawley , Randall Reiserer , Mark Stremler
发明人： John Wikswo , Franz Baudenbacher , Alex Prokop , Eugene Leboeuf , Chang Chung , David Cliffel , Frederick Haselton , William Hofmeister , Charles Lin , Lisa McCawley , Randall Reiserer , Mark Stremler
IPC分类号： C12M1/34
CPC分类号： B01L3/502761 , B01L3/502746 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2300/0877 , B01L2300/10 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , C12M23/16 , C12M29/10
摘要： A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.

3. 发明申请

US20060099705A1 Bioreactors with an array of chambers and a common feed line 有权
标题翻译：具有一系列腔室和共同进料管线的生物反应器
公开(公告)号：US20060099705A1
公开(公告)日：2006-05-11
申请号：US10525538
申请日：2003-08-27
申请人： John Wikswo , David Cliffel , Eugene Leboeuf , Randall Reiserer
发明人： John Wikswo , David Cliffel , Eugene Leboeuf , Randall Reiserer
IPC分类号： C12M1/34
CPC分类号： C12M23/16 , B01L3/5027 , B01L2200/10 , B01L2300/0645 , B01L2300/0681 , B01L2300/0816 , B01L2300/163 , B01L2400/0472 , C12M23/12 , C12M29/10 , C12M41/00 , Y10S435/808
摘要： A bioreactor (200) for cultivating living cells in a liquid medium. In one embodiment, the bioreactor (200) has a substrate (230) having a first surface and an opposite second surface, defining a channel (202) therein, and a plurality of chambers (204) formed in the substrate, wherein each of the plurality of chambers (204) is adapted for receiving cells in a liquid medium and formed with an open end (262), an opposite closed end (264) and side walls, the open end (262) and the closed end (264) defining a depth, d, therebetween for the corresponding chamber (206), the sidewalls defining a width, w, therebetween for the corresponding chamber (206). As such formed, each chamber (206) is in fluid communication with the channel (202) through the open end of the chamber (206), and at least two of the plurality of chambers (206) have depths or widths the same or different form each other.
摘要翻译：一种用于在液体培养基中培养活细胞的生物反应器（200）。在一个实施例中，生物反应器（200）具有衬底（230），其具有在其中限定通道（202）的第一表面和相对的第二表面以及形成在衬底中的多个腔室（204），其中多个腔室（204）适于在液体介质中接收细胞并形成有开口端（262），相对的封闭端（264）和侧壁，开口端（262）和闭合端（264）限定其间的深度d用于相应的腔室（206），所述侧壁在其间为相应的腔室（206）限定宽度w。这样形成，每个室（206）通过腔室（206）的开口端与通道（202）流体连通，并且多个腔室（206）中的至少两个具有相同或不同的深度或宽度形成对方

4. 发明申请

US20060154361A1 Bioreactors with substance injection capacity 有权
标题翻译：具有物质注入能力的生物反应器
公开(公告)号：US20060154361A1
公开(公告)日：2006-07-13
申请号：US10525648
申请日：2003-08-27
申请人： John Wikswo , Franz Baudenbacher , Frederick Haselton , William Hofmeister , Charles Lin , Lisa McCawley , Mark Stremler , Alissa Weaver
发明人： John Wikswo , Franz Baudenbacher , Frederick Haselton , William Hofmeister , Charles Lin , Lisa McCawley , Mark Stremler , Alissa Weaver
IPC分类号： C12M3/00 , C12M3/06
CPC分类号： B01L3/502761 , B01L3/502723 , B01L3/502746 , B01L2200/0668 , B01L2300/0645 , B01L2300/0681 , B01L2300/0816 , B01L2300/0887 , B01L2300/10 , B01L2400/0633 , B01L2400/086 , C12M23/16 , C12M25/14 , C12M29/04 , C12M29/10 , C12M35/08 , C12M41/46
摘要： A bioreactor with substance injection capability. In one embodiment, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a chamber for receiving cells and a liquid medium. A port is formed in the second substrate between the bottom surface and the first surface of the second substrate. As formed, the port is in fluid communication with the chamber to allow a stream of substance to be introduced into the chamber. The stream of substance is controlled so as to provide a gradient, or a concentration gradient of the substance, to the chamber. The stream of substance includes a substance affecting the growth of cells such as chemokine.
摘要翻译：具有物质注入能力的生物反应器。在一个实施例中，生物反应器包括具有第一表面，相对的第二表面和边缘的第一基底。生物反应器还包括具有第一表面和相对的第二表面的第二基底，其限定具有底表面的空腔，其中底表面位于第一表面和第二表面之间。第一基板的第一表面被第二基板的第二表面接收以覆盖空腔，以形成用于容纳电池和液体介质的室。在第二基板的第二基板的底表面和第一表面之间形成端口。如此形成的，端口与腔室流体连通，以允许物质流被引入腔室。物质流被控制以便向腔室提供物质的梯度或浓度梯度。物质流包括影响细胞生长的物质，如趋化因子。

5. 发明申请

US20060073539A1 Apparatus and methods for using biological material to discriminate an agent 审中-公开
标题翻译：使用生物材料鉴别药剂的装置和方法
公开(公告)号：US20060073539A1
公开(公告)日：2006-04-06
申请号：US10212918
申请日：2002-08-06
申请人： John Wikswo , Franz Baudenbacher , R. Balcarcel , Theodore Bapty , David Cliffel , Sven Eklund , Owen McGuinness , Todd Monroe , Ales Prokop , Mark Stremler , Andreas Werdich , Yang Yuansheng
发明人： John Wikswo , Franz Baudenbacher , R. Balcarcel , Theodore Bapty , David Cliffel , Sven Eklund , Owen McGuinness , Todd Monroe , Ales Prokop , Mark Stremler , Andreas Werdich , Yang Yuansheng
IPC分类号： C12Q1/02
CPC分类号： G01N33/5005 , C12M41/32 , G01N33/5023 , G01N33/5038 , G01N33/5041 , G01N2015/1006 , Y10T436/115831
摘要： An apparatus and methods for using biological material to discriminate an agent. In one embodiment of the present invention, the method includes the steps of providing at least one cell, exposing at least one cell to an agent, measuring the response of the cell to the agent in terms of a physical quantity related to at least one of the cellular physiological activities of the cell, and identifying the agent from the measured response. The method further includes the step of quantifying the agent from the measured response.
摘要翻译：用于使用生物材料来鉴别试剂的装置和方法。在本发明的一个实施方案中，该方法包括以下步骤：提供至少一个细胞，将至少一个细胞暴露于药剂，根据与下列中的至少一个相关的物理量测量细胞对药剂的反应：细胞的细胞生理活性，以及根据测定的反应鉴定药剂。该方法还包括从所测量的响应量化试剂的步骤。

6. 发明申请

US20050014129A1 Device and methods for detecting the response of a plurality of cells to at least one analyte of interest 有权
标题翻译：用于检测多个细胞对至少一种感兴趣分析物的响应的装置和方法
公开(公告)号：US20050014129A1
公开(公告)日：2005-01-20
申请号：US10483466
申请日：2002-08-06
申请人： David Cliffel , Franz Baudenbacher , John Wikswo , Sven Eklund , R. Balcarcel , Jonathan Gilligan
发明人： David Cliffel , Franz Baudenbacher , John Wikswo , Sven Eklund , R. Balcarcel , Jonathan Gilligan
IPC分类号： C12M1/34 , C12Q1/00 , C12Q1/68 , C12Q1/70 , G01F1/64 , G01N15/10 , G01N33/48 , G01N33/50 , G06F19/00
CPC分类号： G01N33/5005 , C12M41/32 , G01N33/5023 , G01N33/5038 , G01N33/5041 , G01N2015/1006 , Y10T436/115831
摘要： An apparatus and methods for detecting at least one analyte of interest either produced or consumed by a plurality of cell. In one embodiment of the present invention, the method includes the steps of providing a housing defining a chamber, placing a plurality of cells in the chamber, and simultaneously detecting at least two analytes of interest either produced or consumed by the plurality of cells in the chamber.
摘要翻译：一种用于检测由多个细胞产生或消耗的至少一种感兴趣分析物的装置和方法。在本发明的一个实施例中，该方法包括以下步骤：提供限定腔室的壳体，将多个细胞放置在腔室中，同时检测至少两种感兴趣的分析物，所述分析物是由多个细胞产生或消耗的房间。

7. 发明申请

US20070197900A1 Magnetic flow cytometer with SQUID microscopy 审中-公开
标题翻译：具有SQUID显微镜的磁流式细胞仪
公开(公告)号：US20070197900A1
公开(公告)日：2007-08-23
申请号：US11604018
申请日：2006-11-22
申请人： Franz Baudenbacher , Luis Fong , Eduardo Lima , David Schaffer , John Wikswo
发明人： Franz Baudenbacher , Luis Fong , Eduardo Lima , David Schaffer , John Wikswo
IPC分类号： A61B5/05
CPC分类号： G01R33/0354 , G01N33/54333
摘要： A flow cytometer. In one embodiment, the flow cytometer has a microfluidic structure defining a channel with a periodically modulated path for transporting a stream of fluid with magnetic particles along the modulated path, and a superconducting quantum interference device (SQUID) sensor positioned over the microfluidic structure to define a detecting zone in the microfluidic structure for detecting magnetic signatures of a magnetic particle passing along the periodically modulated path through the detecting zone, where in use the stream of fluid with magnetic particles is regulated such that each magnetic particle passes singly along the periodically modulated path through the detecting zone.
摘要翻译：流式细胞仪在一个实施方案中，流式细胞计数器具有限定具有周期性调制路径的通道的微流体结构，用于沿着调制路径输送具有磁性颗粒的流体流，以及定位在微流体结构上方的超导量子干涉装置（SQUID）微流体结构中的检测区域，用于检测通过检测区域沿着周期性调制路径的磁性粒子的磁特征，其中在使用中调节具有磁性颗粒的流体流，使得每个磁性颗粒沿着周期性调制路径单独通过通过检测区。

8. 发明申请

US20050158845A1 Device and methods for measuring the response of a least one cell to a medium 审中-公开
标题翻译：用于测量至少一个细胞对培养基的响应的装置和方法
公开(公告)号：US20050158845A1
公开(公告)日：2005-07-21
申请号：US10483461
申请日：2002-08-06
申请人： John Wikswo , David Osterman , Franz Baudenbacher , Owen McGuinness , Ales Prokop
发明人： John Wikswo , David Osterman , Franz Baudenbacher , Owen McGuinness , Ales Prokop
IPC分类号： C12M1/34 , C12Q1/00 , C12Q1/68 , C12Q1/70 , G01F1/64 , G01N15/10 , G01N33/48 , G01N33/50 , G06F19/00
CPC分类号： G01N33/5005 , C12M41/32 , G01N33/5023 , G01N33/5038 , G01N33/5041 , G01N2015/1006 , Y10T436/115831
摘要： An apparatus and methods for measuring response of at least one cell to a medium, the response of at least one cell to a medium being characterized by a reaction time. In one embodiment of the present invention, the device includes a membrane and a side substrate defining a sample well in communication with the ambient air and for receiving the at least one cell such that the membrane is underneath the at least one cell. A sensor is positioned underneath the second surface of the membrane, and an inlet is in fluid communication with the sample well. A medium is introduced into the chamber through the inlet to form a droplet that surrounds the cell, and the sensor measures the response of the cell to the medium at a time period shorter than the reaction time.
摘要翻译：用于测量至少一个细胞对培养基的响应的装置和方法，至少一个细胞对介质的响应的特征在于反应时间。在本发明的一个实施例中，该装置包括膜和侧基板，其限定与环境空气连通的样品阱，并且用于接收至少一个电池，使得膜位于至少一个电池的下方。传感器位于膜的第二表面下方，并且入口与样品阱流体连通。通过入口将介质引入室中以形成围绕电池的液滴，并且传感器在比反应时间短的时间段测量电池对介质的响应。

9. 发明申请

US20050153276A1 System and methods for discriminating an agent 审中-公开
标题翻译：用于辨别代理的系统和方法
公开(公告)号：US20050153276A1
公开(公告)日：2005-07-14
申请号：US10510500
申请日：2002-08-06
申请人： John Wikswo , Theodore Bapty , Franz Baudenbacher , Owen McGuinness , Ales Prokop , R. Balcarcel
发明人： John Wikswo , Theodore Bapty , Franz Baudenbacher , Owen McGuinness , Ales Prokop , R. Balcarcel
IPC分类号： C12M1/34 , C12Q1/00 , C12Q1/68 , C12Q1/70 , G01F1/64 , G01N15/10 , G01N33/48 , G01N33/50 , G06F19/00
CPC分类号： G01N33/5005 , C12M41/32 , G01N33/5023 , G01N33/5038 , G01N33/5041 , G01N2015/1006 , Y10T436/115831
摘要： A system and methods for discriminating an agent. In one embodiment of the present invention, the method includes the steps of (a) constructing a decision tree having a plurality of branches, each branch corresponding to at least one defined action, wherein each branch includes a plurality of successive branches, each successive branch corresponding to at least one defined action, (b) providing a conditioned environment sensitive to the agent, (c) obtaining data from response of the agent to the conditioned environment, (d) extracting features from the obtained data, (e) selecting a branch from the decision tree corresponding to the features, (f) performing on the features at least one defined action corresponding to the branch, (g) producing a classification of the agent, and (g) iteratively repeating steps of (d)-(g) until the agent is discriminated.
摘要翻译：用于区分代理的系统和方法。在本发明的一个实施例中，该方法包括以下步骤：（a）构建具有多个分支的决策树，每个分支对应于至少一个定义的动作，其中每个分支包括多个连续的分支，每个相继的分支对应于至少一个定义的动作，（b）提供对代理敏感的条件环境，（c）从代理对响应环境的响应获得数据，（d）从所获得的数据中提取特征，（e）（f）对所述特征执行至少一个与所述分支相对应的定义的动作，（g）产生所述代理的分类，以及（g）迭代重复步骤（d） - （ g）直到代理人受到歧视。

10. 发明申请

US20050153273A1 Device and methods for monitoring the status of at least one cell 有权
标题翻译：用于监视至少一个单元的状态的设备和方法
公开(公告)号：US20050153273A1
公开(公告)日：2005-07-14
申请号：US10755639
申请日：2004-01-12
申请人： John Wikswo , Franz Baudenbacher , Owen McGuinness
发明人： John Wikswo , Franz Baudenbacher , Owen McGuinness
IPC分类号： C12M1/34 , C12Q1/00 , C12Q1/68 , C12Q1/70 , G01F1/64 , G01N15/10 , G01N33/48 , G01N33/50 , G06F19/00
CPC分类号： G01N33/5005 , C12M41/32 , G01N33/5023 , G01N33/5038 , G01N33/5041 , G01N2015/1006 , Y10T436/115831
摘要： A device and methods for monitoring status of at least one cell, wherein the cell has a membrane forming a substantially enclosed structure and defining an intracellular space therein. In one embodiment of the present invention, the device includes a first substrate having a first surface and an opposite second surface, a second substrate supported by the first substrate, the second substrate having a first surface, an opposite second surface, a body portion between the first surface and the second surface, a first side surface and an opposite second side surface, wherein the body portion defines a first passage between the first side surface and the second side surface and an opening on the first surface of the second substrate and in fluid communication with the first passage, and sidewalls positioned above the first surface of the second substrate. In one operation mode, when a first medium is introduced into the first passage, the intracellular space of the cell is in fluid communication with the first passage with the first medium, a sensor measures the response of the cell to the first medium.
摘要翻译：一种用于监测至少一个细胞状态的装置和方法，其中所述细胞具有形成基本封闭结构并在其中限定细胞内空间的膜。在本发明的一个实施例中，该装置包括具有第一表面和相对的第二表面的第一基底，由第一基底支撑的第二基底，第二基底具有第一表面，相对的第二表面，第一表面和第二表面，第一侧表面和相对的第二侧表面，其中主体部分限定在第一侧表面和第二侧表面之间的第一通道和在第二基板的第一表面上的开口，与第一通道流体连通，以及位于第二基板的第一表面上方的侧壁。在一种操作模式中，当第一介质被引入第一通道时，电池的细胞内空间与第一介质与第一通道流体连通，传感器测量电池对第一介质的响应。

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