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    • 1. 发明授权
    • Methods and compositions for regulating cell death and enhancing disease resistance to plant pathogens
    • 用于调节细胞死亡并增强对植物病原体的抗病性的方法和组合物
    • US06455297B1
    • 2002-09-24
    • US09923654
    • 2001-08-07
    • Gurmukh S. JohalSteven P. BriggsJohn GrayGongshe Hu
    • Gurmukh S. JohalSteven P. BriggsJohn GrayGongshe Hu
    • C12N988
    • C12N15/8261C12N9/88C12N15/8242C12N15/8243C12N15/8263C12N15/8282C12N15/8289Y02A40/146
    • Compositions and methods for enhancing disease resistance to a pathogen in a plant are provided. Methods of the invention comprise stably transforming a plant with an antisense nucleotide sequence for a gene encoding an enzyme in the C-5 porphyrin metabolic pathway and operably linking said antisense sequence to a pathogen-inducible promoter, such that invasion of a cell by a pathogen elicits a hypersensitive-like response that results in confinement of the pathogen to cells of initial contact. Transformed plants and seeds are provided. Nucleotide sequences encoding a wild-type maize urod gene useful in the present invention and the amino acid sequence for the protein encoded thereby are provided. These compositions are also useful for regulating cell death in specifically targeted tissues. A maize lesion mimic, dominant mutant phenotype, designated Les22, and the molecular basis for its manifestation are also provided.
    • 提供了用于提高植物中对病原体的抗病性的组合物和方法。 本发明的方法包括用编码C-5卟啉代谢途径中的酶的基因的反义核苷酸序列稳定转化植物,并将所述反义序列可操作地连接到病原体诱导型启动子,使得病原体侵入细胞 引起过敏反应,导致病原体与初始接触细胞的限制。 提供了转化的植物和种子。 提供了编码本发明中有用的野生型玉米urod基因的核苷酸序列和由此编码的蛋白质的氨基酸序列。 这些组合物也可用于调节特异性靶向组织中的细胞死亡。 还提供了玉米病变模拟物,显性突变体表型,命名为Les22,以及其表现的分子基础。
    • 3. 发明授权
    • Methods and compositions for regulating cell death and enhancing disease resistance to plant pathogens
    • 用于调节细胞死亡并增强对植物病原体的抗病性的方法和组合物
    • US06271439B1
    • 2001-08-07
    • US09260843
    • 1999-03-02
    • Gurmukh S. JohalSteven P. BriggsJohn GrayGongshe Hu
    • Gurmukh S. JohalSteven P. BriggsJohn GrayGongshe Hu
    • C12N1529
    • C12N15/8261C12N9/88C12N15/8242C12N15/8243C12N15/8263C12N15/8282C12N15/8289Y02A40/146
    • Compositions and methods for enhancing disease resistance to a pathogen in a plant are provided. Methods of the invention comprise stably transforming a plant with an antisense nucleotide sequence for a gene encoding an enzyme in the C-5 porphyrin metabolic pathway and operably linking the antisense sequence to a pathogen-inducible promoter, such that invasion of a cell by a pathogen elicits a hypersensitive-like response that results in confinement of the pathogen to cells of initial contact. Transformed plants and seeds are provided. Nucleotide sequences encoding a wild-type maize urod gene useful in the present invention and the amino acid sequence for the protein encoded thereby are provided. These compositions are also useful for regulating cell death in specifically targeted tissues. A maize lesion mimic, dominant mutant phenotype, designated Les22, and the molecular basis for its manifestation are also provided.
    • 提供了用于提高植物中对病原体的抗病性的组合物和方法。 本发明的方法包括用编码C-5卟啉代谢途径中的酶的基因的反义核苷酸序列稳定转化植物,并将反义序列可操作地连接到病原体诱导型启动子,使得病原体侵入细胞 引起过敏反应,导致病原体与初始接触细胞的限制。 提供了转化的植物和种子。 提供了编码本发明中有用的野生型玉米urod基因的核苷酸序列和由此编码的蛋白质的氨基酸序列。 这些组合物也可用于调节特异性靶向组织中的细胞死亡。 还提供了玉米病变模拟物,显性突变体表型,命名为Les22,以及其表现的分子基础。
    • 8. 发明申请
    • ACTUATOR WITH GROOVED PISTON FOR A FLUID INFUSION DEVICE
    • 具有用于流体输液装置的活塞活塞的致动器
    • US20120248151A1
    • 2012-10-04
    • US13493888
    • 2012-06-11
    • John Gray
    • John Gray
    • G01F11/00
    • A61M5/14216A61M5/14236A61M5/14276A61M5/168F04B13/00F04B17/048
    • An apparatus for delivering a fluid includes a housing, an inlet in the housing for receiving the fluid, and an outlet in the housing for discharging the fluid. A piston channel is provided within the housing through which the fluid flows from the inlet to the outlet. An actuator is positioned within the housing and is moveable between a retracted position and a forward position, the actuator defining a piston chamber for storing fluid received through the inlet when the actuator is in the retracted position and for driving the fluid stored in the piston chamber toward the outlet when the actuator transitions from the retracted position to the forward position. The actuator includes an armature and a piston coupled to the armature and moveable within the piston channel. The piston is provided with a groove in an outer surface for conducting fluid from the inlet to the outlet.
    • 用于输送流体的装置包括壳体,用于容纳流体的壳体中的入口以及用于排出流体的壳体中的出口。 活塞通道设置在壳体内,流体通过该活塞通道从入口流到出口。 致动器定位在壳体内并且可在缩回位置和向前位置之间移动,致动器限定活塞室,用于当致动器处于缩回位置时存储通过入口接收的流体,并用于驱动储存在活塞室中的流体 当致动器从缩回位置转移到前进位置时朝向出口。 致动器包括电枢和联接到电枢并可在活塞通道内移动的活塞。 活塞在外表面设置有用于将流体从入口传导到出口的凹槽。
    • 10. 发明授权
    • Infusion device and driving mechanism for same
    • 输液器和驱动机构相同
    • US07785293B2
    • 2010-08-31
    • US11170826
    • 2005-06-29
    • John GrayRobert W. Bosley
    • John GrayRobert W. Bosley
    • A61M1/00F04B17/04F04B49/06
    • A61M5/14276A61M5/14216F04B17/042F04B53/1032
    • A drive mechanism for delivery of infusion medium has a coil and an armature moveable toward a forward position, in response to the electromagnetic field produced by activation of the coil. A piston is moveable axially within a piston channel to a forward position, in response to movement of the armature to its forward position. The armature and piston are moved toward a retracted position, when the coil is not energized. In the retracted position of the piston, a piston chamber is formed between the piston and a valve member and is filled with infusion medium. As the piston is moved to its forward position, the piston chamber volume is reduced and pressure within the piston chamber increases to a point where the pressure moves the valve member into an open position through an outlet.
    • 用于输送输注介质的驱动机构具有线圈和衔铁,其可响应于由线圈的激活产生的电磁场朝着向前位置移动。 响应于电枢到其向前位置的运动,活塞可以在活塞通道内轴向移动到前进位置。 当线圈未通电时,电枢和活塞向收回位置移动。 在活塞的缩回位置,活塞室形成在活塞和阀构件之间,并且填充有输注介质。 当活塞移动到其前部位置时,活塞室容积减小,并且活塞室内的压力增加到压力将阀构件通过出口移动到打开位置的点。