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1. 发明授权

US10184134B2 Soybean event SYHT0H2 and compositions and methods for detection thereof 有权
公开(公告)号：US10184134B2
公开(公告)日：2019-01-22
申请号：US13994145
申请日：2011-12-09
申请人： John Daniel Hipskind , Kristina Burgin , Rakesh Jain , Karolyn Terpstra , Marina Sigareva , Annick Jeanne De Framond , Becky Breitinger , Vance Cary Kramer , Weining Gu
发明人： John Daniel Hipskind , Kristina Burgin , Rakesh Jain , Karolyn Terpstra , Marina Sigareva , Annick Jeanne De Framond , Becky Breitinger , Vance Cary Kramer , Weining Gu
IPC分类号： A01H5/10 , C12N15/82 , A01N41/10 , A01N57/20 , C12Q1/6895 , A01N37/40 , A01N39/04
摘要： Soybean plants comprising event SYHT0H2, methods of detecting and using the same, and soybean plants comprising a heterologous insert at the same site as SYHT0H2.

2. 发明申请

US20140201860A1 SOYBEAN EVENT SYHT0H2 AND COMPOSITIONS AND METHODS FOR DETECTION THEREOF 审中-公开
标题翻译：大豆事件SYHT0H2及其检测方法及其检测方法
公开(公告)号：US20140201860A1
公开(公告)日：2014-07-17
申请号：US13994145
申请日：2011-12-09
申请人： John Daniel Hipskind , Kristina Burgin , Rakesh Jain , Karolyn Terpstra , Marina Sigareva , Annick Jeanne De Framond , Becky Breitinger , Vance Cary Kramer , Weining Gu
发明人： John Daniel Hipskind , Kristina Burgin , Rakesh Jain , Karolyn Terpstra , Marina Sigareva , Annick Jeanne De Framond , Becky Breitinger , Vance Cary Kramer , Weining Gu
IPC分类号： C12N15/82 , A01N41/10 , A01N57/20 , A01H5/10 , C12Q1/68
CPC分类号： C12N15/8274 , A01H5/10 , A01N37/40 , A01N39/04 , A01N41/10 , A01N57/20 , C12N15/8281 , C12N15/8282 , C12N15/8283 , C12N15/8286 , C12Q1/6895 , A01N43/40 , A01N43/56 , A01N43/80 , A01N2300/00
摘要： Soybean plants comprising event SYHT0H2, methods of detecting and using the same, and soybean plants comprising a heterologous insert at the same site as SYHT0H2.
摘要翻译：包括事件SYHT0H2的大豆植物，检测和使用其的方法，以及包含与SYHT0H2相同位点的异源插入物的大豆植物。

3. 发明授权

US09121033B2 Polynucleotides encoding trehalose-6-phosphate phosphatase and methods of use thereof 有权
标题翻译：编码海藻糖-6-磷酸磷酸酶的多核苷酸及其使用方法
公开(公告)号：US09121033B2
公开(公告)日：2015-09-01
申请号：US13549794
申请日：2012-07-16
申请人： Kateri Duncan , Michael L. Nuccio , Weining Gu
发明人： Kateri Duncan , Michael L. Nuccio , Weining Gu
IPC分类号： C12N15/82 , C07H21/04 , C12N5/04 , C12N15/00 , C12N9/16 , C12Q1/68
CPC分类号： C12N15/8273 , C12N9/16 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Y204/01216
摘要： The present invention relates generally to the field of molecular biology and regards various polynucleotides, polypeptides and methods that may be employed to enhance yield in transgenic plants. Specifically the transgenic plants may exhibit any one of the traits consisting of increased yield, increased tolerance to abiotic stress, increased cell growth and increased nutrient use efficiency.
摘要翻译：本发明一般涉及分子生物学领域，并涉及可用于增加转基因植物产量的各种多核苷酸，多肽和方法。具体地说，转基因植物可以表现出任何一个性状，其特征包括增加的产量，增加的对非生物胁迫的耐受性，增加的细胞生长和增加的营养利用效率。

4. 发明授权

US07235711B2 Plant cell plastid transformation method using dual selection and producing transplastomic plants without antibiotic resistance genes 有权
标题翻译：植物细胞质体转化方法使用双选择和生产没有抗生素抗性基因的转基因植物
公开(公告)号：US07235711B2
公开(公告)日：2007-06-26
申请号：US10680824
申请日：2003-10-07
申请人： Eric Boudreau , Weining Gu , Anic Deframond , Peter Heifetz
发明人： Eric Boudreau , Weining Gu , Anic Deframond , Peter Heifetz
IPC分类号： C12N15/82
CPC分类号： C12N15/8214 , C12N15/8274
摘要： The present invention relates to the field of plastid transformation. The invention provides transformation vectors and methods to obtain transplastomic plants or algae having a transformed plastid comprising the steps of introducing into plastids a recombinant nucleic acid molecule or vector, and two phases of selection with first selection phase using a non-lethal compound and a second selection phase using a lethal compound. Alternatively, the dual selection method is conducted simultaneously using a lower concentration of the lethal compound.
摘要翻译：本发明涉及质体转化领域。本发明提供了获得具有转化质体的转管植物或藻类的转化载体和方法，包括以下步骤：向质体中引入重组核酸分子或载体，并且使用非致死化合物和第二选择阶段选择两相选择阶段使用致死化合物。或者，使用较低浓度的致死化合物同时进行双重选择方法。

5. 发明授权

US06490670B1 Method and apparatus for efficiently allocating objects in object oriented systems 失效
标题翻译：在面向对象系统中有效分配对象的方法和装置
公开(公告)号：US06490670B1
公开(公告)日：2002-12-03
申请号：US09065407
申请日：1998-04-24
申请人： Michael Thomas Collins , James Lyle Peterson , Weining Gu
发明人： Michael Thomas Collins , James Lyle Peterson , Weining Gu
IPC分类号： G06F1200
CPC分类号： G06F12/023
摘要： A method and apparatus for managing memory allocation. Each memory block category contains memory blocks. A request, including an object size, is received to allocate memory to an object. An available memory block is allocated to the object if the memory block category for size corresponding to the object size of the object contains an available memory block. An available memory block from a memory block category having a memory block size larger than the object size is located if an available memory block is absent in the memory block category for sizes corresponding to the object size. The located available memory block is partitioned into memory blocks, having a size corresponding to the object size. A partitioned memory block from the partitioned memory blocks is allocated to the object.
摘要翻译：一种用于管理存储器分配的方法和装置。每个内存块类别都包含内存块。接收包括对象大小的请求，以将内存分配给对象。如果与对象的对象大小相对应的大小的内存块类别包含可用的内存块，则可用的内存块被分配给该对象。如果对应于对象大小的大小，则在存储器块类别中不存在可用存储器块的情况下，定位具有大于对象大小的存储器块大小的存储器块类别的可用存储器块。所定位的可用存储块被分割成具有对应于对象大小的大小的存储块。分配的存储块的分区存储块被分配给对象。

6. 发明授权

US06858777B2 Methods for stable transformation of plants 有权
标题翻译：植物稳定转化的方法
公开(公告)号：US06858777B2
公开(公告)日：2005-02-22
申请号：US09928614
申请日：2001-08-13
申请人： Heng Zhong , Eric Boudreau , Sabrina Rouse , Erik Dunder , Weining Gu , Yin-Fu Chang
发明人： Heng Zhong , Eric Boudreau , Sabrina Rouse , Erik Dunder , Weining Gu , Yin-Fu Chang
IPC分类号： A01H5/00 , A01H4/00 , C12N5/10 , C12N15/09 , C12N15/82 , C12N15/84
CPC分类号： C12N15/8214 , A01H4/005 , C12N15/8201 , C12N15/8205
摘要： Multiple shoot structures are induced from plant tissues (e.g., shoot apices or axillary buds on an artificial medium) to produce multiple shoot cultures. These multi-shoot cultures are then transformed by known transformation methods. Plants are subsequently regenerated from the transformed cells. Crops that may be efficiently transformed by this method include plants normally recalcitrant to transformation such as sugar beet, sunflower, soybean, cotton, tobacco, tomato, peanuts, melons, watermelon, squash, Brassica, and pepper.
摘要翻译：从植物组织（例如在人造培养基上的枝顶或腋芽）诱导多个芽结构以产生多个芽培养物。然后通过已知的转化方法转化这些多芽培养物。随后从转化细胞再生植物。可以通过该方法有效地转化的作物包括通常难以转化的植物，例如甜菜，向日葵，大豆，棉花，烟草，番茄，花生，甜瓜，西瓜，南瓜，芸苔和胡椒。

7. 发明授权

US5981840A Methods for agrobacterium-mediated transformation 失效
标题翻译：农杆菌介导转化方法
公开(公告)号：US5981840A
公开(公告)日：1999-11-09
申请号：US788018
申请日：1997-01-24
申请人： Zuo-Yu Zhao , Weining Gu , Tishu Cai , Dorothy A. Pierce
发明人： Zuo-Yu Zhao , Weining Gu , Tishu Cai , Dorothy A. Pierce
IPC分类号： C12N15/82 , C12N15/84 , C12N15/00 , A01H5/00 , C12N15/05
CPC分类号： C12N15/8205
摘要： The present invention relates to methods for improving the transformation frequency of Agrobacterium-mediated transformation of maize embryos. A preferred method for transforming maize using Agrobacterium comprises the steps of: contacting at least one immature embryo from a maize plant with Agrobacterium capable of transferring at least one gene to said embryo; co-cultivating the embryos with Agrobacterium; culturing the embryos in medium comprising N6 salts, an antibiotic capable of inhibiting the growth of Agrobacterium, and a selective agent to select for embryos expressing the gene; and regenerating plants expressing the gene.
摘要翻译：本发明涉及改善农杆菌介导的玉米胚转化的转化频率的方法。使用农杆菌转化玉米的优选方法包括以下步骤：使来自玉米植物的至少一种未成熟胚与能够将至少一种基因转移至所述胚的农杆菌接触; 与土壤杆菌共培养胚胎; 在包含N6盐的培养基中培养胚胎，能够抑制农杆菌生长的抗生素，以及选择表达基因的胚胎的选择剂; 和再生表达该基因的植物。

8. 发明授权

US06822144B1 Methods for Agrobacterium-mediated transformation 失效
标题翻译：农杆菌介导转化方法
公开(公告)号：US06822144B1
公开(公告)日：2004-11-23
申请号：US08963096
申请日：1997-11-03
申请人： Zuo-Yu Zhao , Weining Gu , Tishu Cai , Dorothy A. Pierce
发明人： Zuo-Yu Zhao , Weining Gu , Tishu Cai , Dorothy A. Pierce
IPC分类号： C12N1584
CPC分类号： C12N15/8205
摘要： The present invention relates to methods for improving the transformation frequency of Agrobacterium-mediated transformation of maize embryos. A preferred method for transforming maize using Agrobacterium comprises the steps of: contacting at least one immature embryo from a maize plant with Agrobacterium capable of transferring at least one gene to said embryo; co-cultivating the embryos with Agrobacterium; culturing the embryos in medium comprising N6 salts, an antibiotic capable of inhibiting the growth of Agrobacterium, and a selective agent to select for embryos expressing the gene; and regenerating plants expressing the gene.
摘要翻译：本发明涉及改善农杆菌介导的玉米胚转化的转化频率的方法。使用农杆菌转化玉米的优选方法包括以下步骤：使来自玉米植物的至少一种未成熟胚与能够将至少一种基因转移至所述胚的农杆菌接触; 与土壤杆菌共培养胚胎; 在包含N6盐的培养基中培养胚胎，能够抑制农杆菌生长的抗生素，以及选择表达基因的胚胎的选择剂; 和再生表达该基因的植物。

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