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1. 发明授权

US06969598B2 Methods for producing high titre vectors and compositions used in such methods 有权
标题翻译：用于生产高滴度载体和用于这些方法的组合物的方法
公开(公告)号：US06969598B2
公开(公告)日：2005-11-29
申请号：US10134643
申请日：2002-04-30
申请人： John C. Olsen , Kyriacos Andreou Mitrophanous , Jonathan Rohll , Alan John Kingsman , Fiona Margaret Ellard
发明人： John C. Olsen , Kyriacos Andreou Mitrophanous , Jonathan Rohll , Alan John Kingsman , Fiona Margaret Ellard
IPC分类号： A61K48/00 , C07K14/11 , C12N5/08 , C12N5/10 , C12N7/01 , C12N7/08 , C12N15/867 , C12P21/06 , C07H21/02 , C12N5/00 , C12N15/00 , C12Q1/70
CPC分类号： C12N7/00 , A61K48/00 , C12N15/86 , C12N2740/15043 , C12N2740/15052 , C12N2740/15061 , C12N2740/15064 , C12N2740/16134 , C12N2800/22 , C12N2830/002 , C12N2830/003 , C12N2830/48
摘要： A method for producing viral vectors is described using packaging and producer cell lines is described. The producer cell comprises: (i) a first nucleotide sequence (NS) encoding a toxic viral envelope protein operably linked to a promoter; wherein the promoter is operably linked to at least one copy of a TRE; (ii) a second NS wherein the second NS comprises a sequence encoding a tetracycline modulator; (iii) a third NS encoding a retrovirus nucleocapsid protein; and (iv) a fourth NS comprising a retroviral sequence capable of being encapsidated in the nucleocapsid protein such that the retroviral vector particle titre obtainable from the producer cell is regulatable by tetracycline and an initial stimulus with sodium butyrate or functional analogues thereof.
摘要翻译：使用包装描述生产病毒载体的方法，描述生产细胞系。生产细胞包括：（i）编码与启动子可操作地连接的毒性病毒包膜蛋白的第一核苷酸序列（NS）其中所述启动子可操作地连接至TRE的至少一个拷贝; （ii）第二NS，其中所述第二NS包含编码四环素调节剂的序列; （iii）编码逆转录病毒核衣壳蛋白的第三NS; 和（iv）第四NS，其包含能够被包裹在核壳蛋白中的逆转录病毒序列，使得可从生殖细胞获得的逆转录病毒载体颗粒滴度由四环素调节，并且用丁酸钠或其功能类似物进行初始刺激。

2. 发明授权

US06521457B2 Lentivirus-based gene transfer vectors 有权
标题翻译：基于慢病毒的基因转移载体
公开(公告)号：US06521457B2
公开(公告)日：2003-02-18
申请号：US09900419
申请日：2001-07-06
申请人： John C. Olsen
发明人： John C. Olsen
IPC分类号： C12N15867
CPC分类号： C12N15/86 , A61K48/00 , C12N7/00 , C12N2740/15043 , C12N2740/15052
摘要： A recombinant lentiviral vector expression system comprises a first vector that comprises a nucleic acid sequence of at least part of the Equine Infectious Anemia Virus (EIAV) genome. The vector contains at least one defect in at least one gene encoding an EIAV structural protein, but is preferably a gag/pol expression vector. The expression system further comprises a second vector, also comprising a nucleic acid sequence of at least part of the Equine Infectious Anemia Virus (EIAV) genome, and additionally containing a multiple cloning site wherein a heterologous gene may be inserted. The expression system also comprises a third vector which expresses a viral envelope protein. The first and third vectors are packaging signal-defective. When the expression system is transfected into a lentivirus-permissive cell, replication-defective EIAV particles may be produced, which particles are useful in delivering heterologous genes to a broad range of both dividing and non-dividing cells.
摘要翻译：重组慢病毒载体表达系统包含第一载体，其包含至少部分马传染性贫血病毒（EIAV）基因组的核酸序列。该载体在编码EIAV结构蛋白的至少一个基因中含有至少一个缺陷，但优选为gag / pol表达载体。表达系统还包含第二载体，其还包含至少部分马传染性贫血病毒（EIAV）基因组的核酸序列，并另外含有可以插入异源基因的多克隆位点。表达系统还包含表达病毒包膜蛋白的第三载体。第一和第三载体是包装信号有缺陷的。当将表达系统转染入慢病毒允许细胞时，可以产生复制缺陷型EIAV颗粒，该颗粒可用于将异源基因递送到分裂细胞和非分裂细胞的广泛范围。

3. 发明授权

US06277633B1 Lentivirus-based gene transfer vectors 失效
标题翻译：基于慢病毒的基因转移载体
公开(公告)号：US06277633B1
公开(公告)日：2001-08-21
申请号：US09076707
申请日：1998-05-12
申请人： John C. Olsen
发明人： John C. Olsen
IPC分类号： C12N1509
CPC分类号： C12N15/86 , A61K48/00 , C12N7/00 , C12N2740/15043 , C12N2740/15052
摘要： A recombinant lentiviral vector expression system comprises a first vector that comprises a nucleic acid sequence of at least part of the Equine Infectious Anemia Virus (EIAV) genome. The vector contains at least one defect in at least one gene encoding an EIAV structural protein, but is preferably a gaglpol expression vector. The expression system further comprises a second vector, also comprising a nucleic acid sequence of at least part of the Equine Infectious Anemia Virus (EIAV) genome, and additionally containing a multiple cloning site wherein a heterologous gene may be inserted. The expression system also comprises a third vector which expresses a viral envelope protein. The first and third vectors are packaging signal-defective. When the expression system is transfected into a lentivirus-permissive cell, replication-defective EIAV particles may be produced, which particles are useful in delivering heterologous genes to a broad range of both dividing and non-dividing cells.
摘要翻译：重组慢病毒载体表达系统包含第一载体，其包含至少部分马传染性贫血病毒（EIAV）基因组的核酸序列。该载体在编码EIAV结构蛋白的至少一个基因中含有至少一个缺陷，但优选为gaglpol表达载体。表达系统还包含第二载体，其还包含至少部分马传染性贫血病毒（EIAV）基因组的核酸序列，并另外含有可以插入异源基因的多克隆位点。表达系统还包含表达病毒包膜蛋白的第三载体。第一和第三载体是包装信号有缺陷的。当将表达系统转染入慢病毒允许细胞时，可以产生复制缺陷型EIAV颗粒，该颗粒可用于将异源基因递送到分裂细胞和非分裂细胞的广泛范围。

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