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1. 发明申请

US20080269473A1 Method 审中-公开
标题翻译：方法
公开(公告)号：US20080269473A1
公开(公告)日：2008-10-30
申请号：US12077802
申请日：2008-03-20
申请人： Alan John Kingsman , Narry Kim , Ekaterini Kotsopoulou , Jonathan Rohll , Kyriacos A. Mitrophanous
发明人： Alan John Kingsman , Narry Kim , Ekaterini Kotsopoulou , Jonathan Rohll , Kyriacos A. Mitrophanous
IPC分类号： C07H21/00
CPC分类号： C12N7/00 , A61K48/00 , C07K14/005 , C12N15/86 , C12N2740/15022 , C12N2740/15043 , C12N2740/15052 , C12N2740/16043 , C12N2740/16122 , C12N2800/22 , C12N2830/50
摘要： A method of producing a replication defective retrovirus comprising transfecting a producer cell with the following: iii) a retroviral genome; iv) a nucleotide sequence coding for retroviral gag and pol proteins; and iii) nucleotide sequences encoding other essential viral packaging components not encoded by the nucleotide sequence of (ii); characterised in that the nucleotide sequence coding for retroviral gag and pol proteins is codon optimised for expression in the producer cell.
摘要翻译：一种产生复制缺陷型逆转录病毒的方法，包括用以下转染生产细胞：iii）逆转录病毒基因组; iv）编码逆转录病毒gag和pol蛋白的核苷酸序列; 和iii）编码不由（ii）的核苷酸序列编码的其它基本病毒包装组分的核苷酸序列; 其特征在于编码逆转录病毒gag和pol蛋白的核苷酸序列被密码子优化用于在生产细胞中表达。

2. 发明授权

US06312682B1 Retroviral vectors 有权
标题翻译：逆转录病毒载体
公开(公告)号：US06312682B1
公开(公告)日：2001-11-06
申请号：US09224014
申请日：1998-12-28
申请人： Alan John Kingsman , Susan Mary Kingsman , Narry Kim , Kyriacos Mitrophanous
发明人： Alan John Kingsman , Susan Mary Kingsman , Narry Kim , Kyriacos Mitrophanous
IPC分类号： A61K4800
CPC分类号： C12N15/86 , A61K48/00 , C12N2740/16043 , C12N2740/16052
摘要： Retroviral vector production systems for producing lentivirus-based vector particles which are capable of infecting and transducing non-dividing target cells, wherein one or more of the auxiliary genes such as vpr, vif, tat, and nef in the case of HIV-1 are absent from the system. The systems and resulting retrovirus vector particles have improved safety over existing systems and vectors.
摘要翻译：用于产生能够感染和转导不分裂靶细胞的慢病毒载体颗粒的逆转录病毒载体生产系统，其中在HIV-1的情况下，一种或多种辅助基因如vpr，vif，tat和nef是缺席系统。系统和产生的逆转录病毒载体颗粒具有比现有系统和载体更好的安全性。

3. 发明授权

US06235522B1 Lentiviral vectors 有权
标题翻译：慢病毒载体
公开(公告)号：US06235522B1
公开(公告)日：2001-05-22
申请号：US09284011
申请日：1999-04-05
申请人： Alan John Kingsman , Susan Mary Kingsman
发明人： Alan John Kingsman , Susan Mary Kingsman
IPC分类号： C12N1500
CPC分类号： C12N15/86 , A61K48/00 , C12N2740/16043 , C12N2740/16052 , C12N2830/00 , C12N2830/15 , C12N2830/50 , C12N2830/60
摘要： Retroviral vector particles capable of infecting and transducing non-dividing mammalian target cells, which vector particles may be based on letiviruses such as HIV and which have an RNA genome constructed so as to provide in the DNA provirus a non-lentiviral expression control element in the 5′LTR of the provirus.
摘要翻译：能够感染和转导非分裂哺乳动物靶细胞的逆转录病毒载体颗粒，该载体颗粒可以基于诸如HIV之类的病毒性病毒，并且其具有构建的RNA基因，以便在DNA原病毒中提供非慢病毒表达控制元件前病毒的5'LTR。

4. 发明授权

US06132731A Murine leukemia virus vectors 失效
标题翻译：鼠白血病病毒载体
公开(公告)号：US06132731A
公开(公告)日：2000-10-17
申请号：US930503
申请日：1997-10-08
申请人： Alan John Kingsman
发明人： Alan John Kingsman
IPC分类号： C12N15/09 , A61K35/76 , A61K48/00 , C07K14/15 , C07K14/47 , C07K14/705 , C07K16/28 , C12N7/00 , C12N7/01 , C12N7/04 , C12N15/12 , C12N15/867 , C12N15/87 , C12P21/00 , C12R1/91 , C07H21/02
CPC分类号： C07K16/2842 , C07K14/005 , C07K14/47 , C07K14/70542 , C12N15/86 , C12N15/87 , C12N7/00 , C07K2317/76 , C07K2319/00 , C12N2740/13022 , C12N2740/13043 , C12N2740/13045 , C12N2740/13062 , C12N2810/405 , C12N2810/854 , C12N2810/855
摘要： A proteinaceous particle comprises a capsid enveloped by ecotropic Murine Leukemia virus envelope proteins characterized in that a heterologous peptide which binds to a non-murine cell is inserted in, entirely replaces, or replaces a portion of the native Ser-Gly-Gly-Ser-Ser-Pro-Gly of the VRA region of said envelope proteins. A method for preparing a plurality of such proteinaceous particles comprises expressing within a host cell (i) self-assembling capsid proteins, (ii) Murine Leukemia virus envelope proteins, said ENV proteins modified as defined, and optionally, (iii) packageable RNA, and then culturing the host cells and harvesting the resultant budded particles.
摘要翻译： PCT No.PCT / GB96 / 00776 Sec。 371日期1997年10月8日第 102（e）日期1997年10月8日PCT 1996年4月1日PCT PCT。出版物WO96 / 31602 日期1996年10月10日蛋白质颗粒包括由亲和性鼠白血病病毒包膜蛋白包围的衣壳，其特征在于将与非鼠细胞结合的异源肽插入，完全替代或取代一部分天然Ser-Gly -Gly-Ser-Ser-Pro-Gly的所述包膜蛋白的VRA区。制备多种这样的蛋白质颗粒的方法包括在宿主细胞内表达（i）自组装衣壳蛋白，（ii）鼠白血病病毒包膜蛋白，所定义的修饰的所述ENV蛋白，以及任选地，（iii）可包装的RNA，然后培养宿主细胞并收获所产生的发芽颗粒。

5. 发明授权

US07790419B2 Antiviral vectors 有权
标题翻译：抗病毒载体
公开(公告)号：US07790419B2
公开(公告)日：2010-09-07
申请号：US10351938
申请日：2003-01-27
申请人： Alan John Kingsman , Kyriacos Mitrophanous , Narry Kim
发明人： Alan John Kingsman , Kyriacos Mitrophanous , Narry Kim
IPC分类号： C12N15/00 , C12N15/64
CPC分类号： C12N15/1132 , C12N15/63 , C12N15/86 , C12N2310/111 , C12N2740/13022 , C12N2740/16043
摘要： A viral vector production system is provided which system comprises: (i) a viral genome comprising at least one first nucleotide sequence encoding a gene product capable of binding to and effecting the cleavage, directly or indirectly, of a second nucleotide sequence, or transcription product thereof, encoding a viral polypeptide required for the assembly of viral particles; (ii) a third nucleotide sequence encoding said viral polypeptide required for the assembly of the viral genome into viral particles, which third nucleotide sequence has a different nucleotide sequence to the second nucleotide sequence such that said third nucleotide sequence, or transcription product thereof, is resistant to cleavage directed by said gene product. The viral vector production system may be used to produce viral particles for use in treating or preventing viral infection.
摘要翻译：提供病毒载体生产系统，其系统包括：（i）病毒基因组，其包含至少一个编码能够直接或间接地结合并实现第二核苷酸序列或转录产物的切割的基因产物的第一核苷酸序列编码病毒颗粒装配所需的病毒多肽; （ii）编码病毒基因组装入病毒颗粒所需的所述病毒多肽的第三核苷酸序列，该第三核苷酸序列与第二核苷酸序列具有不同的核苷酸序列，使得所述第三核苷酸序列或其转录产物是耐受由所述基因产物引导的切割。病毒载体生产系统可用于产生用于治疗或预防病毒感染的病毒颗粒。

6. 发明授权

US07259015B2 Vector system 有权
标题翻译：矢量系统
公开(公告)号：US07259015B2
公开(公告)日：2007-08-21
申请号：US10408456
申请日：2003-04-07
申请人： Alan John Kingsman , Nicholas D. Mazarakis , Enca Martin-Rendon , Mimoun Azzouz , Jonathan Rohll
发明人： Alan John Kingsman , Nicholas D. Mazarakis , Enca Martin-Rendon , Mimoun Azzouz , Jonathan Rohll
IPC分类号： C12N15/867 , C12N15/63 , C12N15/64 , C12N5/10 , A61K48/00
CPC分类号： C12N15/86 , A61K48/00 , C12N9/0071 , C12N2740/15043 , C12N2830/48 , C12N2840/206
摘要： Provided are retroviral vector genomes and vector systems comprising the genomes. In particular, a retroviral vector genome comprising two or more NOIs, operably linked by one or more Internal Ribosome Entry Site(s); a lentiviral vector genome comprising two or more NOIs suitable for treating a neurodegenerative disorder; and a lentiviral vector genome which encodes tyrosine hydroxylase, GTP-cyclohydrolase I and, optionally, Aromatic Amino Acid Dopa Decarboxylase are provided.
摘要翻译：提供了包含基因组的逆转录病毒载体基因组和载体系统。特别地，包含由一个或多个内部核糖体进入位点可操作地连接的两个或更多个NOI的逆转录病毒载体基因组; 包含两种或更多种适用于治疗神经变性疾病的NOI的慢病毒载体基因组; 并提供编码酪氨酸羟化酶，GTP-环化水解酶I和任选的芳香族氨基酸多巴脱羧酶的慢病毒载体基因组。

7. 发明授权

US06808922B1 Retroviral vectors comprising a functional splice donor site and a functional splice acceptor site 失效
标题翻译：包含功能性剪接供体位点和功能性剪接受体位点的逆转录病毒载体
公开(公告)号：US06808922B1
公开(公告)日：2004-10-26
申请号：US09508516
申请日：2000-06-08
申请人： Christopher Robert Bebbington , Susan Mary Kingsman , Mark Uden , Alan John Kingsman , Kyriacos Mitrophanos
发明人： Christopher Robert Bebbington , Susan Mary Kingsman , Mark Uden , Alan John Kingsman , Kyriacos Mitrophanos
IPC分类号： C12N1563
CPC分类号： C12N15/63 , A61K48/00 , C07K14/005 , C12N15/86 , C12N2710/10343 , C12N2740/13043 , C12N2740/15022 , C12N2740/15043 , C12N2760/20122 , C12N2810/40 , C12N2810/60 , C12N2810/6054 , C12N2830/002 , C12N2830/42 , C12N2830/48 , C12N2830/50 , C12N2840/203 , C12N2840/44
摘要： A retroviral vector is described. The retroviral vector comprises a functional splice donor site and a functional splice acceptor site; wherein the functional splice donor site and the functional splice acceptor site flank a first nucleotide sequence of interest (“NOI”); wherein the functional splice donor site is upstream of the functional splice acceptor site; wherein the retroviral vector is derived from a retroviral pro-vector; wherein the retroviral pro-vector comprises a first nucleotide sequence (“NS”) capable of yielding the functional splice donor site and a second NS capable of yielding the functional splice acceptor site; wherein the first NS is downstream of the second NS; such that the retroviral vector is formed as a result of reverse transcription of the retroviral pro-vector.
摘要翻译：描述了逆转录病毒载体。逆转录病毒载体包含功能性剪接供体位点和功能性剪接受体位点; 其中所述功能性剪接供体位点和所述功能性剪接受体位点位于所述第一核苷酸序列（“NOI”）的两侧。其中所述功能性接合供体位点在所述功能性接头受体位点的上游; 其中所述逆转录病毒载体衍生自逆转录病毒载体; 其中所述逆转录病毒载体包含能够产生所述功能性剪接供体位点的第一核苷酸序列（“NS”）和能产生所述功能性剪接受体位点的第二NS; 其中所述第一NS在所述第二NS的下游; 使得逆转录病毒载体由于逆转录病毒载体的逆转录而形成。

8. 发明申请

US20090291491A1 Retroviral vectors 审中-公开
公开(公告)号：US20090291491A1
公开(公告)日：2009-11-26
申请号：US12286545
申请日：2008-09-30
申请人： Alan John Kingsman , Susan Mary Kingsman , Narry Kim , Kyriacos Mitrophanous
发明人： Alan John Kingsman , Susan Mary Kingsman , Narry Kim , Kyriacos Mitrophanous
IPC分类号： C12N15/00
CPC分类号： C12N15/86 , A61K48/00 , A61K48/0091 , C12N2740/15043 , C12N2740/15052 , C12N2740/16043 , C12N2740/16052
摘要： Retroviral vector production systems for producing lentivirus-based vector particles which are capable of infecting and transducing non-dividing target cells, wherein one or more of the auxiliary genes such as vpr, vif, tat, and nef in the case of HIV-1 are absent from the system. The systems and resulting retrovirus vector particles have improved safety over existing systems and vectors.

9. 发明授权

US06969598B2 Methods for producing high titre vectors and compositions used in such methods 有权
标题翻译：用于生产高滴度载体和用于这些方法的组合物的方法
公开(公告)号：US06969598B2
公开(公告)日：2005-11-29
申请号：US10134643
申请日：2002-04-30
申请人： John C. Olsen , Kyriacos Andreou Mitrophanous , Jonathan Rohll , Alan John Kingsman , Fiona Margaret Ellard
发明人： John C. Olsen , Kyriacos Andreou Mitrophanous , Jonathan Rohll , Alan John Kingsman , Fiona Margaret Ellard
IPC分类号： A61K48/00 , C07K14/11 , C12N5/08 , C12N5/10 , C12N7/01 , C12N7/08 , C12N15/867 , C12P21/06 , C07H21/02 , C12N5/00 , C12N15/00 , C12Q1/70
CPC分类号： C12N7/00 , A61K48/00 , C12N15/86 , C12N2740/15043 , C12N2740/15052 , C12N2740/15061 , C12N2740/15064 , C12N2740/16134 , C12N2800/22 , C12N2830/002 , C12N2830/003 , C12N2830/48
摘要： A method for producing viral vectors is described using packaging and producer cell lines is described. The producer cell comprises: (i) a first nucleotide sequence (NS) encoding a toxic viral envelope protein operably linked to a promoter; wherein the promoter is operably linked to at least one copy of a TRE; (ii) a second NS wherein the second NS comprises a sequence encoding a tetracycline modulator; (iii) a third NS encoding a retrovirus nucleocapsid protein; and (iv) a fourth NS comprising a retroviral sequence capable of being encapsidated in the nucleocapsid protein such that the retroviral vector particle titre obtainable from the producer cell is regulatable by tetracycline and an initial stimulus with sodium butyrate or functional analogues thereof.
摘要翻译：使用包装描述生产病毒载体的方法，描述生产细胞系。生产细胞包括：（i）编码与启动子可操作地连接的毒性病毒包膜蛋白的第一核苷酸序列（NS）其中所述启动子可操作地连接至TRE的至少一个拷贝; （ii）第二NS，其中所述第二NS包含编码四环素调节剂的序列; （iii）编码逆转录病毒核衣壳蛋白的第三NS; 和（iv）第四NS，其包含能够被包裹在核壳蛋白中的逆转录病毒序列，使得可从生殖细胞获得的逆转录病毒载体颗粒滴度由四环素调节，并且用丁酸钠或其功能类似物进行初始刺激。

10. 发明授权

US06541219B1 Therapeutic Gene 有权
标题翻译：治疗基因
公开(公告)号：US06541219B1
公开(公告)日：2003-04-01
申请号：US09254832
申请日：1999-06-21
申请人： Alan John Kingsman , Susan Mary Kingsman
发明人： Alan John Kingsman , Susan Mary Kingsman
IPC分类号： C12N15867
CPC分类号： C12N9/0071 , A61K48/00 , C07K2319/00 , C12N9/88 , C12N15/62 , C12N2799/027
摘要： Polynucleotide sequences and vectors containing them, for use in gene therapy, the polynucleotide sequences comprising two or more therapeutic genes operably linked to a promoter and encoding a fusion protein product of the therapeutic genes. The fusion protein may be for example a tyrosine hydroxylase (TH)-DOPA decarboxylase (DD) fusion in either TH-DD or DD-TH order, useful for treating Parkinson's disease.
摘要翻译：含有它们的多核苷酸序列和载体用于基因治疗，所述多核苷酸序列包含与启动子可操作地连接并编码治疗基因的融合蛋白产物的两个或更多个治疗基因。融合蛋白可以是例如TH-DD或DD-TH序列中用于治疗帕金森病的酪氨酸羟化酶（TH）-DOPA脱羧酶（DD）融合物。

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