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1. 发明授权

US07204922B1 Method and device for the electrophoretic separation of particles, especially of macromolecules, by electrophoresis 失效
标题翻译：通过电泳电泳分离颗粒，特别是大分子的方法和装置
公开(公告)号：US07204922B1
公开(公告)日：2007-04-17
申请号：US10049245
申请日：1999-07-26
申请人： Johan-Valentin Kahl , Julia Nissen , Joachim Rädler , Roman Zantl , Berenike Maier , Ulf Rädler , Andres Hohner , Reinhard Galneder
发明人： Johan-Valentin Kahl , Julia Nissen , Joachim Rädler , Roman Zantl , Berenike Maier , Ulf Rädler , Andres Hohner , Reinhard Galneder
IPC分类号： G01N27/26
CPC分类号： G01N27/44773 , B01D57/02 , G01N27/44747
摘要： The present invention provides a method for the electrophoretic separation of particles, especially of macromolecules, comprising the steps of applying the particles to be separated on a substrate supported membrane, such that the particles are mobile across the surface of the substrate supported membrane; providing an electrical field such that electrical fields are formed along the surface across which the particles are mobile; and temporarily modifying the electrical field and/or adding a substrate supported membrane having a structured surface, wherein the direction and/or the strength of the electrical field are temporarily modified and/or wherein the substrate supported membrane is structured so that a force is acting on the particles that leads to a movement depending on the length of the particles. Moreover, a substrate is provided, in particular, for supporting a membrane during the performing of the inventive methods, that comprises an optically transparent material. Moreover, the invention provides a substrate supported membrane for carrying out the inventive methods including an inventive substrate as defined in the claims and a fluid lipid membrane. Moreover, the present invention provides a microchannel electrophoresis chamber having at least one channel with a bottom surface including an inventive substrate, and an electrode assembly.
摘要翻译：本发明提供了一种用于电泳分离颗粒，特别是大分子的方法，包括以下步骤：将待分离的颗粒施加在基板支撑的膜上，使得颗粒在基底支撑的膜的表面上是可移动的; 提供电场，使得沿着颗粒可移动的表面形成电场; 并临时修改电场和/或添加具有结构化表面的基底支撑膜，其中电场的方向和/或强度被暂时改变和/或其中基底支撑膜被构造成使得力作用在导致根据颗粒长度的运动的颗粒上。此外，提供了一种衬底，特别是用于在执行本发明的方法期间支撑膜，其包括光学透明材料。此外，本发明提供了一种用于实施本发明方法的底物负载膜，包括如权利要求中所定义的本发明基质和流体脂质膜。此外，本发明提供一种具有至少一个通道的微通道电泳室，底部表面包括本发明的基底和电极组件。

2. 发明授权

US07582264B2 Device for microfluid analyses 有权
标题翻译：微流体分析装置
公开(公告)号：US07582264B2
公开(公告)日：2009-09-01
申请号：US10589307
申请日：2004-10-04
申请人： Johan-Valentin Kahl , Roman Zantl
发明人： Johan-Valentin Kahl , Roman Zantl
IPC分类号： B01L11/00
CPC分类号： G02B21/34 , B01L3/502753 , B01L2300/047 , B01L2300/0681 , B01L2300/0822 , G01N2001/4016 , G01N2013/003
摘要： The invention relates to a device for microfluid analyses for a substrate with plane base surface and cover surface, wherein a chamber is integrated in the substrate for receiving liquid with at least two admissions and a semipermeable or permeable membrane is arranged in the chamber, wherein the chamber is subdivided by the membrane into two sectional chambers with at least one admission each.
摘要翻译：本发明涉及一种用于具有平面基面和盖表面的基底的微流体分析装置，其中腔室集成在基底中，用于接收具有至少两次注入的液体，并且半透膜或渗透膜布置在腔室中，其中腔室被膜细分为两个分区室，每个腔室至少有一个入口。

3. 发明申请

US20060239865A1 Sample chamber for a liquid 有权
标题翻译：用于液体的样品室
公开(公告)号：US20060239865A1
公开(公告)日：2006-10-26
申请号：US10542455
申请日：2004-01-13
申请人： Johan-Valentin Kahl , Roman Zantl
发明人： Johan-Valentin Kahl , Roman Zantl
IPC分类号： B01L9/00
CPC分类号： B01L3/502723 , B01D19/0031 , B01L3/5027 , B01L3/502746 , B01L2200/0684 , B01L2300/069 , B01L2300/0822 , B01L2300/10 , B01L2400/0487
摘要： The present invention relates to a sample chamber for a liquid, wherein at least one part in the interior of the sample chamber comprises a fluid-absorbent and/or fluid-permeable plastic, which is essentially degasified in at least one area and/or in which a predetermined fluid and/or predetermined solid is stored in soluble form in at least one area.
摘要翻译：本发明涉及一种用于液体的样品室，其中样品室内部的至少一部分包括流体吸收和/或流体可渗透的塑料，其在至少一个区域和/或其中预定流体和/或预定固体以可溶形式存储在至少一个区域中。

4. 发明授权

US07799282B2 Sample chamber for a liquid 有权
标题翻译：用于液体的样品室
公开(公告)号：US07799282B2
公开(公告)日：2010-09-21
申请号：US10542455
申请日：2004-01-13
申请人： Johan-Valentin Kahl , Roman Zantl
发明人： Johan-Valentin Kahl , Roman Zantl
IPC分类号： B01D53/04 , B01D19/00 , B01L3/00
CPC分类号： B01L3/502723 , B01D19/0031 , B01L3/5027 , B01L3/502746 , B01L2200/0684 , B01L2300/069 , B01L2300/0822 , B01L2300/10 , B01L2400/0487
摘要： The present invention relates to a sample chamber for a liquid, wherein at least one part in the interior of the sample chamber comprises a fluid-absorbent and/or fluid-permeable plastic, which is essentially degasified in at least one area and/or in which a predetermined fluid and/or predetermined solid is stored in soluble form in at least one area.
摘要翻译：本发明涉及一种用于液体的样品室，其中样品室内部的至少一部分包括流体吸收和/或流体可渗透的塑料，其在至少一个区域和/或其中预定流体和/或预定固体以可溶形式存储在至少一个区域中。

5. 发明授权

US08470110B2 Sample chamber and method for the production thereof 有权
标题翻译：样品室及其制备方法
公开(公告)号：US08470110B2
公开(公告)日：2013-06-25
申请号：US11996546
申请日：2006-07-31
申请人： Johan-Valentin Kahl , Roman Zantl
发明人： Johan-Valentin Kahl , Roman Zantl
IPC分类号： B01L3/00 , B29D23/00 , B29L23/00
CPC分类号： B29C65/02 , B01J2219/00317 , B01J2219/00527 , B01J2219/00605 , B01J2219/0061 , B01J2219/00621 , B01J2219/0063 , B01J2219/00635 , B01L3/502707 , B01L3/5085 , B01L2200/0668 , B01L2200/12 , B01L2300/0636 , B01L2300/0681 , B01L2300/0822 , B01L2300/0877 , B01L2300/0887 , B01L2300/161 , B01L2300/165 , B01L2400/0406 , B01L2400/086 , B29C59/142 , B29C65/16 , B29C65/48 , B29C65/4895 , B29C66/02 , B29C66/1122 , B29C66/345 , B29C66/5342 , B29C66/53421 , B29C66/53461 , B29C66/71 , B29C66/7352 , B29C66/8322 , B29K2023/06 , B29K2023/12 , B29K2025/00 , B29K2033/12 , B29K2067/00 , B29K2069/00 , B29K2071/00 , B29K2077/00 , B29K2995/0026 , Y10T156/10 , B29K2067/003 , B29K2027/18 , B29K2023/38
摘要： The invention relates to a sample chamber with a cover plate and a bottom plate which is joined thereto in a fixed manner, wherein a recess is provided in the cover plate so that a reservoir is formed by the bottom plate, wherein a planar element with holes is provided in the reservoir, wherein the holes have a predetermined arrangement and/or size.
摘要翻译：本发明涉及具有盖板和底板的样品室，其以固定的方式连接到其上，其中在盖板中设置有凹槽，使得由底板形成储存器，其中具有孔的平面元件设置在储存器中，其中孔具有预定的布置和/或尺寸。

6. 发明申请

US20100175488A1 Sample Chamber and Method for the Production Thereof 有权
标题翻译：样品室及其生产方法
公开(公告)号：US20100175488A1
公开(公告)日：2010-07-15
申请号：US11996546
申请日：2006-07-31
申请人： Johan-Valentin Kahl , Roman Zantl
发明人： Johan-Valentin Kahl , Roman Zantl
IPC分类号： B01L9/00 , B32B37/00 , H05H1/24
CPC分类号： B29C65/02 , B01J2219/00317 , B01J2219/00527 , B01J2219/00605 , B01J2219/0061 , B01J2219/00621 , B01J2219/0063 , B01J2219/00635 , B01L3/502707 , B01L3/5085 , B01L2200/0668 , B01L2200/12 , B01L2300/0636 , B01L2300/0681 , B01L2300/0822 , B01L2300/0877 , B01L2300/0887 , B01L2300/161 , B01L2300/165 , B01L2400/0406 , B01L2400/086 , B29C59/142 , B29C65/16 , B29C65/48 , B29C65/4895 , B29C66/02 , B29C66/1122 , B29C66/345 , B29C66/5342 , B29C66/53421 , B29C66/53461 , B29C66/71 , B29C66/7352 , B29C66/8322 , B29K2023/06 , B29K2023/12 , B29K2025/00 , B29K2033/12 , B29K2067/00 , B29K2069/00 , B29K2071/00 , B29K2077/00 , B29K2995/0026 , Y10T156/10 , B29K2067/003 , B29K2027/18 , B29K2023/38
摘要： The invention relates to a sample chamber with a cover plate and a bottom plate which is joined thereto in a fixed manner, wherein a recess is provided in the cover plate so that a reservoir is formed by the bottom plate, wherein a planar element with holes is provided in the reservoir, wherein the holes have a predetermined arrangement and/or size.
摘要翻译：本发明涉及具有盖板和底板的样品室，其以固定的方式连接到其上，其中在盖板中设置有凹槽，使得由底板形成储存器，其中具有孔的平面元件设置在储存器中，其中孔具有预定的布置和/或尺寸。

7. 发明授权

US07517499B2 Flow chamber 有权
标题翻译：流动室
公开(公告)号：US07517499B2
公开(公告)日：2009-04-14
申请号：US10491016
申请日：2002-09-25
申请人： Johan-Valentin Kahl
发明人： Johan-Valentin Kahl
IPC分类号： B01L3/00
CPC分类号： B01L3/5027 , B01L3/5025 , B01L3/502715 , B01L2200/027 , B01L2300/0822 , B01L2300/0877 , B01L2300/12 , B01L2400/0406 , B01L2400/0457 , G02B21/34
摘要： A flow chamber (1) made of plastics as an object carrier for light-microscopic examinations comprised at least one channel (4) in a base plate (2), said channel having a width of 0.01 to 20 mm and a height of 0.01 to 5 mm. One liquid reservoir (3, 3′) each is connected to the inlet and outlet opening (6, 6′) of the channel, whereby a communicating system is generated. The bottom and/or the cover of this chamber is made of a high-class plastic material and may be functionalized. The inlet and outlet portions of the channel may be formed by a rounding (7) of the channel edges or by a surface treatment in a manner that drop formation is prevented and the flow is therefore not obstructed.In a method of sample preparation for light-optical microscopic examinations, a sample flow is generated by a system of communicating pipes, in that a reservoir of a solution with the sample is connected via a third channel with at least one further reservoir and the filling level of the reservoirs differs at the beginning of the examination.
摘要翻译：由塑料制成的流动室（1）作为用于光学显微镜检查的物体载体，其包括在基板（2）中的至少一个通道（4），所述通道的宽度为0.01至20mm，高度为0.01至 5毫米。一个液体储存器（3,3'）各自连接到通道的入口和出口（6,6'），由此产生通信系统。该室的底部和/或盖子由高级塑料材料制成并且可以被功能化。通道的入口和出口部分可以由通道边缘的圆形（7）或通过表面处理形成，使得防止液滴形成并且因此流动不被阻碍。在用于光学显微镜检查的样品制备方法中，通过连通管道的系统产生样品流，其中具有样品的溶液的储存器经由第三通道与至少一个另外的储器连接，并且填充物水库水平在考试开始时有所不同。

8. 发明授权

US08162357B2 Microfluid system connection 有权
标题翻译：微流体系统连接
公开(公告)号：US08162357B2
公开(公告)日：2012-04-24
申请号：US10506028
申请日：2003-02-28
申请人： Johan-Valentin Kahl
发明人： Johan-Valentin Kahl
IPC分类号： F16L25/00
CPC分类号： B01J19/0093 , B01J2219/00813 , B01L3/502715 , B01L2200/027 , B01L2200/0689 , B01L2300/0816 , G01N27/44704 , G01N27/44791 , Y10S285/921
摘要： A microfluid system includes at least one flow-through volume, and at least one connector connected to the flow-through volume. The microfluid system includes a structured surface having at least one elevation. Each elevation is a tapered elevation. Each connector is formed in a respective elevation and is configured as a tapered recess.
摘要翻译：微流体系统包括至少一个流通体积和连接到流通体积的至少一个连接器。微流体系统包括具有至少一个高度的结构化表面。每个高程都是锥形高程。每个连接器形成在相应的高度，并且被构造为锥形凹部。

9. 发明申请

US20070272000A1 Device For Microfluid Analyses 有权
标题翻译：微流体分析装置
公开(公告)号：US20070272000A1
公开(公告)日：2007-11-29
申请号：US10589307
申请日：2004-10-04
申请人： Johan-Valentin Kahl , Roman Zanti
发明人： Johan-Valentin Kahl , Roman Zanti
IPC分类号： B01L11/00
CPC分类号： G02B21/34 , B01L3/502753 , B01L2300/047 , B01L2300/0681 , B01L2300/0822 , G01N2001/4016 , G01N2013/003
摘要： The invention relates to a device for microfluid analyses for a substrate with plane base surface and cover surface, wherein a chamber is integrated in the substrate for receiving liquid with at least two admissions and a semipermeable or permeable membrane is arranged in the chamber, wherein the chamber is subdivided by the membrane into two sectional chambers with at least one admission each.
摘要翻译：本发明涉及一种用于具有平面基面和盖表面的基底的微流体分析装置，其中腔室集成在基底中，用于接收具有至少两次注入的液体，并且半透膜或渗透膜布置在腔室中，其中腔室被膜细分为两个分区室，每个腔室至少有一个入口。

10. 发明申请

US20050118068A1 Microfluid system 有权
标题翻译：微流体系统
公开(公告)号：US20050118068A1
公开(公告)日：2005-06-02
申请号：US10506028
申请日：2003-02-28
申请人： Johan-Valentin Kahl
发明人： Johan-Valentin Kahl
IPC分类号： B01J19/00 , B01L3/00 , G01N27/447
CPC分类号： B01J19/0093 , B01J2219/00813 , B01L3/502715 , B01L2200/027 , B01L2200/0689 , B01L2300/0816 , G01N27/44704 , G01N27/44791 , Y10S285/921
摘要： The present invention relates to a microfluid system comprising at least one flow-through volume and at least one connection means connected to the volume and configured in the form of a tapered recess or a tapered elevation. Furthermore, the invention relates to a plug means for use in such a microfluid system, the plug means being configured such that it can be brought into engagement with the connection means.
摘要翻译：微流体系统本发明涉及一种微流体系统，其包括至少一个流通体积和至少一个连接到该体积并连接成锥形凹槽或锥形高度的连接装置。此外，本发明涉及一种用于这种微流体系统的塞子装置，该塞子装置构造成使其能够与连接装置接合。

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