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1. 发明授权

US5670354A Use of VSV-G pseudotyped vectors for transfer of genes into embryos 失效
标题翻译：使用VSV-G假型载体将基因转移到胚胎中
公开(公告)号：US5670354A
公开(公告)日：1997-09-23
申请号：US480453
申请日：1995-06-07
申请人： Jane C. Burns , Jiing-Kuan Yee , Theodore Friedmann
发明人： Jane C. Burns , Jiing-Kuan Yee , Theodore Friedmann
IPC分类号： C12N5/00 , A61K38/00 , A61K48/00 , C07K14/145 , C12N5/10 , C12N7/00 , C12N7/04 , C12N15/09 , C12N15/867 , C12N15/87 , C12R1/91 , C12N15/00 , C12N5/06 , C12N5/16 , C12N15/06
CPC分类号： C12N15/86 , A61K48/00 , C07K14/005 , C12N15/87 , C12N7/00 , A61K38/00 , C12N2740/13043 , C12N2740/13052 , C12N2740/13062 , C12N2760/20222 , C12N2830/002 , C12N2830/42 , C12N2830/60 , C12N2840/20 , C12N2840/203
摘要： The present application discloses retrovirus-derived vectors in which the retroviral envelope glycoprotein has been replaced by the G glycoprotein of vesicular stomatitis virus, and the use of these vectors in the transfer of exogenous genes into the cells of a wide variety of non-mammalian organisms. Also disclosed is a method for the generation of retroviral vectors in high titers, wherein a recombinant, stable host cell line is provided which harbors the retroviral vector of interest without envelope protein. High-titer retroviral vector production is initiated by introducing nucleic acid encoding a functional membrane-associated protein into the cell line. The vectors disclosed in the present application can be concentrated by ultracentrifugation to titers greater than 10.sup.9 cfu/ml which are especially useful in human gene therapy trials, and can also infect cells, such as hamster and fish cells, that are ordinarily resistant to infection with vectors containing the retroviral envelope protein.
摘要翻译：本申请公开了逆转录病毒衍生载体，其中逆转录病毒包膜糖蛋白已经被水泡性口炎病毒的G糖蛋白替代，以及这些载体在将外源基因转移到多种非哺乳动物生物体的细胞中的用途。还公开了用于产生高滴度的逆转录病毒载体的方法，其中提供重组的稳定的宿主细胞系，其含有没有包膜蛋白的感兴趣的逆转录病毒载体。通过将编码功能性膜相关蛋白的核酸引入细胞系来启动高效价逆转录病毒载体产生。本申请中公开的载体可以通过超速离心浓缩至大于109cfu / ml的滴度，这在人类基因治疗试验中特别有用，并且还可以感染通常抗感染的细胞，例如仓鼠和鱼细胞含有逆转录病毒包膜蛋白的载体。

2. 发明授权

US5512421A Generation, concentration and efficient transfer of VSV-G pseudotyped retroviral vectors 失效
标题翻译： VSV-G假型逆转录病毒载体的产生，集中和有效转移
公开(公告)号：US5512421A
公开(公告)日：1996-04-30
申请号：US104804
申请日：1993-08-10
申请人： Jane C. Burns , Jiing-Kuan Yee , Theodore Friedmann
发明人： Jane C. Burns , Jiing-Kuan Yee , Theodore Friedmann
IPC分类号： C12N5/00 , A61K38/00 , A61K48/00 , C07K14/145 , C12N5/10 , C12N7/00 , C12N7/04 , C12N15/09 , C12N15/867 , C12N15/87 , C12R1/91 , C12N15/86 , C12N7/02 , C12N15/47 , C12N15/48
CPC分类号： C12N15/86 , A61K48/00 , C07K14/005 , C12N15/87 , C12N7/00 , A61K38/00 , C12N2740/13043 , C12N2740/13052 , C12N2740/13062 , C12N2760/20222 , C12N2830/002 , C12N2830/42 , C12N2830/60 , C12N2840/20 , C12N2840/203
摘要： The present application discloses retrovirus-derived vectors in which the retroviral envelope glycoprotein has been replaced by the G glycoprotein of vesicular stomatitis virus, and the use of these vectors in the transfer of exogenous genes into the cells of a wide variety of non-mammalian organisms. Also disclosed is a method for the generation of retroviral vectors in high titers, wherein a recombinant, stable host cell line is provided which harbors the retroviral vector of interest without envelope protein. High-titer retroviral vector production is initiated by introducing nucleic acid encoding a functional membrane-associated protein into the cell line. The vectors disclosed in the present application can be concentrated by ultracentrifugation to titers greater than 10.sup.9 cfu/ml which are especially useful in human gene therapy trials, and can also infect cells, such as hamster and fish cells, that are ordinarily resistant to infection with vectors containing the retroviral envelope protein.
摘要翻译：本申请公开了逆转录病毒衍生载体，其中逆转录病毒包膜糖蛋白已经被水泡性口炎病毒的G糖蛋白替代，以及这些载体在将外源基因转移到多种非哺乳动物生物体的细胞中的用途。还公开了用于产生高滴度的逆转录病毒载体的方法，其中提供重组的稳定的宿主细胞系，其含有没有包膜蛋白的感兴趣的逆转录病毒载体。通过将编码功能性膜相关蛋白的核酸引入细胞系来启动高效价逆转录病毒载体产生。本申请中公开的载体可以通过超速离心浓缩至大于109cfu / ml的滴度，这在人类基因治疗试验中特别有用，并且还可以感染通常抗感染的细胞，例如仓鼠和鱼细胞含有逆转录病毒包膜蛋白的载体。

3. 发明授权

US07138562B2 Transgenic animals 有权
公开(公告)号：US07138562B2
公开(公告)日：2006-11-21
申请号：US09907859
申请日：2001-07-18
申请人： Robert D. Bremel , Anthony W. S. Chan , Jane C. Burns
发明人： Robert D. Bremel , Anthony W. S. Chan , Jane C. Burns
IPC分类号： C12N15/00 , A01K67/00 , A01K67/033
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2227/101 , A01K2267/01 , A01K2267/02 , C07K14/005 , C12N15/8509 , C12N15/86 , C12N2730/10122 , C12N2740/13043 , C12N2740/13051
摘要： The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.

4. 发明授权

US06548740B1 Methods for creating transgenic animals 有权
标题翻译：创建转基因动物的方法
公开(公告)号：US06548740B1
公开(公告)日：2003-04-15
申请号：US09502264
申请日：2000-02-11
申请人： Robert D. Bremel , Anthony W.S. Chan , Jane C. Burns
发明人： Robert D. Bremel , Anthony W.S. Chan , Jane C. Burns
IPC分类号： C12N1500
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2227/101 , A01K2267/01 , A01K2267/02 , C07K14/005 , C12N15/8509 , C12N15/86 , C12N2730/10122 , C12N2740/13043 , C12N2740/13051
摘要： The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.
摘要翻译：本发明提供用于产生转基因非人动物的改进的方法和组合物。本发明允许通过将感染性逆转录病毒显微注射到卵的periviteline空间中将外源核酸序列引入未受精卵的基因组（例如，预成熟卵母细胞和预受精卵母细胞）中。本发明的方法提供了转基因动物的生产效率的提高，其中产生动物的速率降低，这是为了转基因的存在而镶嵌的。

5. 发明申请

US20110189698A1 Protein Biomarkers and Methods for Diagnosing Kawasaki Disease 审中-公开
标题翻译：蛋白质生物标志物和诊断川崎病的方法
公开(公告)号：US20110189698A1
公开(公告)日：2011-08-04
申请号：US13036751
申请日：2011-02-28
申请人： Jane C. Burns
发明人： Jane C. Burns
IPC分类号： G01N33/68 , G01N33/74 , G01N33/573 , G01N30/00
CPC分类号： G01N33/6893 , G01N2800/328 , G01N2800/60
摘要： A method, kit and device for diagnosing Kawasaki Disease are provided. The invention provides detecting an expression level of at least two Kawasaki Disease diagnostic biomarkers in a biological sample from a patient with a capture agent and diagnosing the patient as having Kawasaki Disease when the expression levels of the at least two diagnostic biomarkers in the patient biological sample are higher than the normal expression levels of the same biomarkers in a biological sample from a control subject. The first Kawasaki Disease diagnostic biomarker disclosed in the present invention is a cardiomyocyte biomarker, and the second Kawasaki Disease diagnostic biomarker is an inflammatory biomarker. The invention further provides detecting an expression level of a third biomarker, interferon type-I biomarker, in the patient biological sample with a capture agent and diagnosing the patient as having Kawasaki Disease when the expression level of interferon type-I biomarker is lower than the expression level in a control subject.
摘要翻译：提供了一种用于诊断川崎病的方法，试剂盒和装置。本发明提供了检测来自患有捕获剂的患者的生物样品中至少两种川崎病诊断生物标志物的表达水平，并且当患者生物样品中至少两种诊断生物标志物的表达水平被诊断为患有川崎病时高于来自对照受试者的生物样品中相同生物标志物的正常表达水平。本发明公开的第一川崎病诊断生物标志物是心肌细胞生物标志物，第二川崎病诊断生物标志物是炎性生物标志物。本发明进一步提供了当患有生物标本的患者生物样品中的第三种生物标志物，干扰素I型生物标志物的表达水平用捕获剂检测，并且当干扰素I型生物标志物的表达水平低于表达水平在对照科目。

6. 发明授权

US06291740B1 Transgenic animals 失效
标题翻译：转基因动物
公开(公告)号：US06291740B1
公开(公告)日：2001-09-18
申请号：US09329749
申请日：1999-06-10
申请人： Robert D. Bremel , Anthony W. S. Chan , Jane C. Burns
发明人： Robert D. Bremel , Anthony W. S. Chan , Jane C. Burns
IPC分类号： C12N1500
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2227/101 , A01K2267/01 , A01K2267/02 , C07K14/005 , C12N15/8509 , C12N15/86 , C12N2730/10122 , C12N2740/13043 , C12N2740/13051
摘要： The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.
摘要翻译：本发明提供用于产生转基因非人动物的改进的方法和组合物。本发明允许通过将感染性逆转录病毒显微注射到卵的periviteline空间中将外源核酸序列引入未受精卵的基因组（例如，预成熟卵母细胞和预受精卵母细胞）中。本发明的方法提供了转基因动物的生产效率的提高，其中产生动物的速率降低，这是为了转基因的存在而镶嵌的。

7. 发明授权

US6080912A Methods for creating transgenic animals 失效
公开(公告)号：US6080912A
公开(公告)日：2000-06-27
申请号：US821984
申请日：1997-03-20
申请人： Robert D. Bremel , Anthony W. S. Chan , Jane C. Burns
发明人： Robert D. Bremel , Anthony W. S. Chan , Jane C. Burns
IPC分类号： A01K67/027 , C07K14/02 , C12N5/02 , C12N15/85 , C12N15/867 , C12N15/00
CPC分类号： A01K67/0275 , C07K14/005 , C12N15/8509 , C12N15/86 , A01K2217/05 , A01K2227/101 , A01K2267/01 , A01K2267/02 , C12N2730/10122 , C12N2740/13043 , C12N2740/13051
摘要： The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.

8. 发明授权

US5969211A Pantropic retroviral vectors for gene transfer in mollusks 失效
标题翻译：用于软体动物基因转移的泛曲逆转录病毒载体
公开(公告)号：US5969211A
公开(公告)日：1999-10-19
申请号：US844530
申请日：1997-04-18
申请人： Jane C. Burns , Thomas T. Chen
发明人： Jane C. Burns , Thomas T. Chen
IPC分类号： A01K67/033 , C07K14/435 , C12N15/867 , C12N5/00 , C12N15/00
CPC分类号： C12N15/86 , A01K67/0334 , C07K14/43563 , A01K2217/05 , C12N2740/13043
摘要： A method for introducing foreign nucleic acid sequences into marine mollusks. A pantropic retroviral vector containing a foreign gene sequence is introduced into fertilized mollusk embryos by electroporation. The gene sequence becomes integrated into the host DNA and encodes a functional protein product. This method has implications in the introduction of disease-resistance and growth-accelerating genes into mollusks.
摘要翻译：将外源核酸序列引入海洋软体动物的方法。通过电穿孔将含有外来基因序列的外翻逆转录病毒载体引入受精的软体动物胚胎。基因序列整合到宿主DNA中，并编码功能蛋白产物。这种方法对于将抗病性和生长促进基因引入软体动物有影响。

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