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    • 8. 发明授权
    • Fused proteins comprising glycoprotein gD of HSV-1 and LTB
    • 包含HSV-1和LTB的糖蛋白gD的融合蛋白
    • US5241053A
    • 1993-08-31
    • US577915
    • 1990-09-05
    • Yukio FujisawaShuji HinumaAki MayumiTatsuo Yamamoto
    • Yukio FujisawaShuji HinumaAki MayumiTatsuo Yamamoto
    • A61K39/00A61K47/48C07K14/035C07K14/245
    • C07K14/245A61K47/4833C07K14/005A61K39/00C07K2319/40C07K2319/55C12N2710/16622
    • Disclosed are (1) a fused protein comprising heat-labile enterotoxin B subunit and a protein heterologous to heat-labile enterotoxin, (2) a recombinant DNA containing a nucleotide sequence coding for the above fused protein, (3) a transformant harboring the above recombinant DNA, (4) a method for producing the fused protein which comprises cultivating the above transformant, producing and accumulating the above fused protein in a culture, and collecting the fused protein, and (5) a method for purifying a fused protein comprising a herpes simplex virus surface antigen and heat-labile enterotoxin B subunit, which comprises cultivating a transformant harboring a recombinant DNA containing a nucleotide sequence coding for the fused protein, producing an accumulating the fused protein in a culture, collecting the fused protein and subjecting the collected fused protein to purification processes comprising cationic exchange chromatography and gel permeation chromatography.
    • 公开了(1)包含热不稳定肠毒素B亚基和与热不稳定肠毒素异源的蛋白质的融合蛋白,(2)含有编码上述融合蛋白的核苷酸序列的重组DNA,(3)含有上述 重组DNA,(4)生产融合蛋白的方法,包括培养上述转化体,在培养物中产生和积累上述融合蛋白,并收集融合蛋白,和(5)纯化融合蛋白的方法, 单纯疱疹病毒表面抗原和热不稳定肠毒素B亚基,其包括培养含有编码融合蛋白的核苷酸序列的重组DNA的转化体,产生在培养物中积聚融合蛋白,收集融合蛋白并使收集的 融合蛋白与包含阳离子交换层析和凝胶渗透色谱的纯化方法。
    • 9. 发明申请
    • Portable Language Learning Device and Portable Language Learning System
    • 便携式语言学习装置和便携式语言学习系统
    • US20070243506A1
    • 2007-10-18
    • US11718754
    • 2005-11-08
    • Daiji IdoYoshifumi YonemotoTakao YamaguchiJunichi SatoIchiro TakeiTatsuo YamamotoHiroshi Takase
    • Daiji IdoYoshifumi YonemotoTakao YamaguchiJunichi SatoIchiro TakeiTatsuo YamamotoHiroshi Takase
    • G09B19/00
    • G09B19/06G09B5/06
    • A portable language learning device which automatically provides language learning data adapted to an event stored in a schedule book of the learner in accordance with the event thereby to improve learning efficiency. The device comprises a schedule storage section (102) where a schedule is stored, a date/time measuring section (103) for measuring the current time and the date, a teaching material storage section (106) where teaching materials associated with a scene or a keyword are stored, a teaching material selecting section (104) for extracting the nearest event corresponding to the measurement by the date/time measuring section (103) from the schedule stored in the schedule storage section (102), comparing the scenes or keywords of the teaching material stored in the teaching material storage section (106), and selecting a teaching material most suitable for the event, and a teaching material reproducing section (105) for reproducing the selected teaching material. The schedule stare section (102) stores a schedule composed of scenes, events, dates, and time information.
    • 一种便携式语言学习装置,其根据事件自动地提供适合于存储在学习者的日程表中的事件的语言学习数据,从而提高学习效率。 该装置包括存储日程表的日程表存储部分(102),用于测量当前时间和日期的日期/时间测量部分(103),教学材料存储部分(106),与教学材料相关的教学材料 存储关键字,教学材料选择部分(104),用于从存储在时间表存储部分(102)中的时间表中提取由日期/时间测量部分(103)对应于测量的最近事件,比较场景或关键字 存储在教学材料存储部分(106)中的教学材料,以及选择最适合于该事件的教学材料,以及用于再现所选教材的教学材料再现部分(105)。 时间表凝视部分(102)存储由场景,事件,日期和时间信息组成的时间表。