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1. 发明申请

US20070160502A1 MICROFLUIDIC DEVICE AND METHOD OF FABRICATING THE SAME 审中-公开
标题翻译：微流体装置及其制造方法
公开(公告)号：US20070160502A1
公开(公告)日：2007-07-12
申请号：US11616148
申请日：2006-12-26
申请人： Kyu-youn Hwang , Sung-young Jeong , Joon-ho KIM , Hun-joo LEE , Hee-kyun LIM
发明人： Kyu-youn Hwang , Sung-young Jeong , Joon-ho KIM , Hun-joo LEE , Hee-kyun LIM
IPC分类号： B32B37/12 , B32B38/04 , B01L3/00
CPC分类号： B01L3/502707 , B01L2200/12 , B01L2300/0816 , B01L2300/0887 , Y10T156/1052
摘要： Disclosed herein are a method of fabricating a microfluidic device, and a microfluidic device fabricated by the method. The method includes coating an adhesive material on a first substrate having a fluid port to form an adhesive layer thereon, arranging a second substrate having a microstructure formed therein with the surface of the first substrate on which the adhesive layer is formed, such that the fluid port and the microstructure correspond to each other, and heating the substrates at about 50 to about 180 degrees Celsius to bind the first substrate to the second substrate.
摘要翻译：本文公开了一种制造微流体装置的方法，以及通过该方法制造的微流体装置。该方法包括在具有流体端口的第一基板上涂覆粘合剂材料以在其上形成粘合剂层，将其中形成有微结构的第二基板与其上形成有粘合剂层的第一基板的表面布置，使得流体端口和微结构彼此对应，并且在约50至约180摄氏度下加热基板以将第一基板结合到第二基板。

2. 发明申请

US20090005265A1 METHOD OF ISOLATING NUCLEIC ACID USING MATERIAL POSITIVELY CHARGED AT FIRST PH AND CONTAINING AMINO GROUP AND CARBOXYL GROUP 有权
标题翻译：使用在第一个PH和含有氨基和羧基的正电荷充填材料分离核酸的方法
公开(公告)号：US20090005265A1
公开(公告)日：2009-01-01
申请号：US12141894
申请日：2008-06-18
申请人： Kyu-youn HWANG , Joon-ho KIM , Chang-eun YOO , Hun-joo LEE , Hee-kyun LIM , Sung-young JEONG , Jeo-young SHIM
发明人： Kyu-youn HWANG , Joon-ho KIM , Chang-eun YOO , Hun-joo LEE , Hee-kyun LIM , Sung-young JEONG , Jeo-young SHIM
IPC分类号： C40B40/04 , C07C63/26
CPC分类号： C12N15/1006 , B01D15/361 , B01D15/364 , B01J20/3285 , B01J43/00 , C12Q1/6806 , C12Q2527/119 , C12Q2523/308
摘要： A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.
摘要翻译：提供了从含有核酸的样品中分离核酸的方法。该方法包括使样品与含有氨基和羧基的双官能材料接触并在第一pH下带正电荷以允许核酸与双功能材料的结合; 以及从所述络合物中提取比所述第一pH高的第二pH的所述核酸。

3. 发明申请

US20080044880A1 METHOD OF AND APPARATUS FOR SEPARATING MICROORGANISMS FROM SAMPLE USING ELECTRODIALYSIS AND MICROORGANISM CAPTURING MEANS 审中-公开
标题翻译：使用电化学和微生物捕获方法从样品中分离微生物的方法和装置
公开(公告)号：US20080044880A1
公开(公告)日：2008-02-21
申请号：US11841191
申请日：2007-08-20
申请人： Jung-im HAN , Joon-ho KIM , Sung-young JEONG , Young-sun LEE , Kyu-youn HWANG , Hun-joo LEE
发明人： Jung-im HAN , Joon-ho KIM , Sung-young JEONG , Young-sun LEE , Kyu-youn HWANG , Hun-joo LEE
IPC分类号： C12N13/00 , C12M1/00
CPC分类号： C12N1/02 , B01D61/48
摘要： A method of separating microorganisms from a sample includes introducing a sample into an apparatus which controls a concentration of at least one salt, the apparatus includes a reaction chamber defined between a cation exchange membrane and an anion exchange membrane, a first electrode chamber defined between the anion exchange membrane and a first electrode, the first electrode chamber containing a first ion exchange medium, a second electrode chamber defined between the cation exchange membrane and a second electrode, the second electrode chamber containing a second ion exchange medium, applying a voltage between the first electrode and the second electrode to electrodialyze the sample in the reaction chamber and reduce the concentration of the at least one salt in the sample and allowing the sample having the reduced concentration of the at least one salt to contact a microorganism capturing means.
摘要翻译：从样品中分离微生物的方法包括将样品引入到控制至少一种盐浓度的装置中，该装置包括界定在阳离子交换膜和阴离子交换膜之间的反应室，第一电极室阴离子交换膜和第一电极，第一电极室包含第一离子交换介质，第二电极室限定在阳离子交换膜和第二电极之间，第二电极室包含第二离子交换介质，第一电极和第二电极，使反应室中的样品电渗析，并降低样品中至少一种盐的浓度，并允许具有降低的至少一种盐浓度的样品接触微生物捕获装置。

4. 发明申请

US20080044864A1 METHOD OF AMPLIFYING NUCLEIC ACID FROM A CELL USING A NONPLANAR SOLID SUBSTRATE 有权
标题翻译：使用非固体基质从细胞中放大核酸的方法
公开(公告)号：US20080044864A1
公开(公告)日：2008-02-21
申请号：US11841107
申请日：2007-08-20
申请人： Sung-young JEONG , Kyu-youn HWANG , Joon-ho KIM , Jung-im HAN , Hun-joo LEE
发明人： Sung-young JEONG , Kyu-youn HWANG , Joon-ho KIM , Jung-im HAN , Hun-joo LEE
IPC分类号： C12P19/34
CPC分类号： C12N15/1006 , B01L7/52 , B01L2300/0636 , C12N2533/30 , C12Q1/6806
摘要： Provided is a method of amplifying nucleic acid from a comprising: contacting a cell-containing sample with a nonplanar solid substrate in a liquid medium having a pH range of 3.0-6.0 to attach the cell to the solid substrate; washing the nonplanar solid substrate to remove materials that are not attached thereto; and performing PCR using the nucleic acid from the cell attached to the nonplanar solid substrate as a template sample to amplify nucleic acid from the cell, wherein the contacting, washing and PCR processes are performed in a single vessel.
摘要翻译：本发明提供一种扩增来自a的核酸的方法，其特征在于：在pH范围为3.0-6.0的液体介质中使含细胞的样品与非平面固体基质接触，以将该细胞附着在固体基质上; 洗涤非平面固体基质以除去未附着于其上的材料; 以及使用来自附着在所述非平面固体底物上的细胞的核酸作为模板样品进行PCR以扩增来自所述细胞的核酸，其中所述接触，洗涤和PCR过程在单个容器中进行。

5. 发明申请

US20070237680A1 MICROFLUIDIC DEVICE AND METHOD FOR CONCENTRATING SAMPLE CONTAINING CELLS OR VIRUSES AND LYSING CELLS OR VIRUSES, AND METHOD OF PRODUCING THE MICROFLUIDIC DEVICE 有权
标题翻译：用于浓缩包含细胞或病毒和感染细胞或病毒的样品的微流体装置和方法以及产生微流体装置的方法
公开(公告)号：US20070237680A1
公开(公告)日：2007-10-11
申请号：US11670002
申请日：2007-02-01
申请人： Hun-joo LEE , Joon-ho KIM , Sung-young JEONG , Kyu-youn HWANG
发明人： Hun-joo LEE , Joon-ho KIM , Sung-young JEONG , Kyu-youn HWANG
IPC分类号： G01N33/00
CPC分类号： C12M47/02 , C12M47/06
摘要： A microfluidic device for concentrating a sample including cells or viruses and lysing the cells or viruses, the device including an anode chamber including an anode electrode, a cathode chamber including a cathode electrode and an ion exchange membrane separating the anode chamber and the cathode chamber. The cathode chamber includes a solid support therein. A method of producing the microfluidic device and a method of concentrating a sample including cells or viruses and lysing the cells or viruses therein using the microfluidic device.
摘要翻译：一种用于浓缩包含细胞或病毒的样品并裂解细胞或病毒的微流体装置，所述装置包括包括阳极电极的阳极室，包括阴极的阴极室和分离阳极室和阴极室的离子交换膜。阴极室包括固体支撑体。微流体装置的制造方法以及浓缩包含细胞或病毒的样品并使用微流体装置使细胞或病毒裂解的方法。

6. 发明申请

US20090159463A1 MICROFLUIDIC DEVICE COMPRISING ELECTROLYSIS DEVICE FOR CELL LYSIS AND METHOD FOR ELECTROCHEMICALLY LYSING CELLS USING THE SAME 有权
标题翻译：包含用于细胞分析的电解装置的微流体装置和使用其的电化学细胞的方法
公开(公告)号：US20090159463A1
公开(公告)日：2009-06-25
申请号：US12349273
申请日：2009-01-06
申请人： Hun-joo LEE , Joon-ho KIM , Chang-eun YOO , Hee-kyun LIM , Kyu-youn Hwang , Soo-min MA , Jun-hong Min
发明人： Hun-joo LEE , Joon-ho KIM , Chang-eun YOO , Hee-kyun LIM , Kyu-youn Hwang , Soo-min MA , Jun-hong Min
IPC分类号： G01N33/50
CPC分类号： C12N1/066 , C12M47/06
摘要： Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.
摘要翻译：提供了一种微流体装置，其包括用于细胞裂解的电解装置，其包括阳极室，阴极室和隔板，其中隔膜安装在阳极室和阴极室之间，阳极室包括入口和出口阳极室溶液和电极，阴极室包括用于阴极室溶液和电极的入口和出口，以及使用其进行电化学裂解细胞的方法。

7. 发明申请

US20070138025A1 MICROFLUIDIC DEVICE FOR ELECTROCHEMICALLY REGULATING pH OF FLUID AND METHOD OF REGULATING pH OF FLUID USING THE MICROFLUIDIC DEVICE 有权
标题翻译：用于电化学调节流体pH的微流体装置和使用微流化装置调节流体pH的方法
公开(公告)号：US20070138025A1
公开(公告)日：2007-06-21
申请号：US11559196
申请日：2006-11-13
申请人： Hun-joo LEE , Joon-ho KIM , Kyu-youn HWANG
发明人： Hun-joo LEE , Joon-ho KIM , Kyu-youn HWANG
IPC分类号： C02F1/461
CPC分类号： G05D21/02
摘要： A microfluidic device includes; an ion exchange membrane, an anode chamber one side of which contacts a surface of the ion exchange membrane, wherein the anode chamber further includes a ladder-shaped anode and an anode support part, and a cathode chamber, one side of which contacts a surface of the ion exchange membrane opposite the anode chamber, wherein the cathode chamber further comprises a cathode, wherein the ladder-shaped anode is formed on a first surface of the anode support part, openings are formed in the anode support part which conform to the shape of the ladder-shaped anode, and a second surface of the anode support part which opposes the first surface of the anode contacts and supports the ion exchange membrane.
摘要翻译：微流体装置包括：离子交换膜，阳极室，其一面接触离子交换膜的表面，其中阳极室还包括梯形阳极和阳极支撑部分，阴极室的一侧接触表面所述阴极室还包括阴极，其中所述梯形阳极形成在所述阳极支撑部分的第一表面上，所述阳极支撑部分形成符合所述形状的阳极支撑部分的形状，并且与阳极的第一表面相对的阳极支撑部分的第二表面接触并支撑离子交换膜。

8. 发明申请

US20120255871A1 APPARATUS AND METHOD FOR ADJUSTING PH OF SOLUTION 有权
标题翻译：用于调节溶液PH的装置和方法
公开(公告)号：US20120255871A1
公开(公告)日：2012-10-11
申请号：US13495342
申请日：2012-06-13
申请人： Hun-joo LEE , Jin-tae KIM , Sung-young JEONG , Joon-ho KIM , Hee-kyun LIM
发明人： Hun-joo LEE , Jin-tae KIM , Sung-young JEONG , Joon-ho KIM , Hee-kyun LIM
IPC分类号： C25B15/00
CPC分类号： G05D21/02
摘要： A pH adjusting apparatus includes an electrolytic chip receiving a solution, an electrolytic chip loading station receiving the electrolytic chip, an input unit inputting electrolysis conditions, a control unit receiving the electrolysis conditions and controlling electrolysis performed in the electrolytic chip, and a display unit displaying the electrolysis conditions and a progress of the electrolysis. Thus, the pH of a solution can be adjusted easily and accurately, by precisely controlling a constant current, a constant voltage, and current and voltage application times, thereby enabling useful application in various biological assays such as cell lysis. Furthermore, the pH adjusting apparatus has small size and weight and can be operated for a long time after charging once due to low power consumption.
摘要翻译： pH调节装置包括：接收溶液的电解芯片，接收电解芯片的电解芯片装载站，输入电解条件的输入单元，接收电解条件的控制单元以及控制电解芯片中进行的电解;以及显示单元，电解条件和电解进度。因此，通过精确地控制恒定电流，恒定电压，电流和电压施加时间，可以容易且精确地调节溶液的pH，从而可用于各种生物测定如细胞裂解中的有用应用。此外，pH调节装置的体积小，重量轻，能够在低功耗的情况下一次充电后长时间运转。

9. 发明申请

US20070151864A1 APPARATUS AND METHOD FOR ADJUSTING PH OF SOLUTION 有权
标题翻译：用于调节溶液PH的装置和方法
公开(公告)号：US20070151864A1
公开(公告)日：2007-07-05
申请号：US11559924
申请日：2006-11-15
申请人： Hun-joo LEE , Jin-tae KIM , Sung-young JEONG , Joon-ho KIM , Hee-kyun LIM
发明人： Hun-joo LEE , Jin-tae KIM , Sung-young JEONG , Joon-ho KIM , Hee-kyun LIM
IPC分类号： C25B1/00
CPC分类号： G05D21/02
摘要： A pH adjusting apparatus includes an electrolytic chip receiving a solution, an electrolytic chip loading station receiving the electrolytic chip, an input unit inputting electrolysis conditions, a control unit receiving the electrolysis conditions and controlling electrolysis performed in the electrolytic chip, and a display unit displaying the electrolysis conditions and a progress of the electrolysis. Thus, the pH of a solution can be adjusted easily and accurately, by precisely controlling a constant current, a constant voltage, and current and voltage application times, thereby enabling useful application in various biological assays such as cell lysis. Furthermore, the pH adjusting apparatus has small size and weight and can be operated for a long time after charging once due to low power consumption.
摘要翻译： pH调节装置包括：接收溶液的电解芯片，接收电解芯片的电解芯片装载站，输入电解条件的输入单元，接收电解条件的控制单元以及控制电解芯片中进行的电解;以及显示单元，电解条件和电解进度。因此，通过精确地控制恒定电流，恒定电压，电流和电压施加时间，可以容易且精确地调节溶液的pH，从而可用于各种生物测定如细胞裂解中的有用应用。此外，pH调节装置的体积小，重量轻，能够在低功耗的情况下一次充电后长时间运转。

10. 发明申请

US20070138012A1 MICROFLUIDIC DEVICE FOR ELECTROCHEMICALLY REGULATING pH OF FLUID AND METHOD OF REGULATING pH OF FLUID USING THE MICROFLUIDIC DEVICE 有权
标题翻译：用于电化学调节流体pH的微流体装置和使用微流化装置调节流体pH的方法
公开(公告)号：US20070138012A1
公开(公告)日：2007-06-21
申请号：US11558940
申请日：2006-11-13
申请人： Hun-joo LEE , Joon-ho KIM , Sung-young JEONG , Hee-kyun LIM
发明人： Hun-joo LEE , Joon-ho KIM , Sung-young JEONG , Hee-kyun LIM
IPC分类号： G01N27/26
CPC分类号： B01D61/44 , B01L3/502707 , B01L2200/0684 , B01L2300/10 , B01L2400/0487
摘要： A microfluidic device which electrochemically regulates a pH of a fluid, and a method of regulating a pH of a fluid using the microfluidic device, include a chamber which includes a cathode formed of a metal adsorbing hydrogen gas, and an anode formed of a metal having a higher standard oxidation potential than, and does not react with, water.
摘要翻译：电化学调节流体pH值的微流体装置以及使用微流体装置调节流体pH值的方法包括：包含由吸附氢气的金属形成的阴极的室和由金属形成的阳极，比水更高的标准氧化电位，并且不与水反应。

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