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1. 发明授权

US09181534B1 High fidelity DNA polymerase compositions and uses thereof 有权
标题翻译：高保真DNA聚合酶组合物及其用途
公开(公告)号：US09181534B1
公开(公告)日：2015-11-10
申请号：US10035091
申请日：2001-12-21
申请人： Holly Hogrefe , Michael Borns , Joseph A. Sorge
发明人： Holly Hogrefe , Michael Borns , Joseph A. Sorge
IPC分类号： C12N9/12 , C12P19/34
CPC分类号： C12N9/12 , C12N9/1252 , C12P19/34
摘要： The subject invention relates to compositions comprising an enzyme mixture which comprises a first enzyme and a second enzyme, where the first enzyme comprises a DNA polymerization activity and the second enzyme comprises an 5′-3′ exonuclease activity and a reduced DNA polymerization activity. The invention also relates to the above compositions in kit format and methods for high fidelity DNA synthesis using the subject compositions of the invention.
摘要翻译：本发明涉及包含含有第一酶和第二酶的酶混合物的组合物，其中第一种酶包含DNA聚合活性，第二种酶包含5'-3'核酸外切酶活性和降低的DNA聚合活性。本发明还涉及使用本发明的主题组合物的用于高保真DNA合成的试剂盒形式的上述组合物和方法。

2. 发明授权

US07932070B2 High fidelity DNA polymerase compositions and uses therefor 有权
标题翻译：高保真DNA聚合酶组合物及其用途
公开(公告)号：US07932070B2
公开(公告)日：2011-04-26
申请号：US10227110
申请日：2002-08-23
申请人： Holly Hogrefe , Michael Borns , Joseph A. Sorge
发明人： Holly Hogrefe , Michael Borns , Joseph A. Sorge
IPC分类号： C12N9/12 , C07K1/00
CPC分类号： C12N9/1252
摘要： The subject invention relates to compositions comprising an enzyme mixture which comprises a first enzyme and a second enzyme, where the first enzyme comprises a DNA polymerization activity and the second enzyme comprises an 3′-5′ exonuclease activity and a reduced DNA polymerization activity. The invention also relates to the above compositions in kit format and methods for high fidelity DNA synthesis using the subject compositions of the invention.
摘要翻译：本发明涉及包含含有第一酶和第二酶的酶混合物的组合物，其中第一种酶包含DNA聚合活性，第二种酶包含3'-5'核酸外切酶活性和降低的DNA聚合活性。本发明还涉及使用本发明的主题组合物的用于高保真DNA合成的试剂盒形式的上述组合物和方法。

3. 发明申请

US20080199935A1 Compositions and methods utilizing DNA polymerases 审中-公开
标题翻译：使用DNA聚合酶的组合物和方法
公开(公告)号：US20080199935A1
公开(公告)日：2008-08-21
申请号：US11644729
申请日：2006-12-22
申请人： Joseph A. Sorge , Holly Hogrefe , Connie J. Hansen
发明人： Joseph A. Sorge , Holly Hogrefe , Connie J. Hansen
IPC分类号： C12N9/12
CPC分类号： B82Y5/00 , B82Y10/00 , C12N9/1252
摘要： The invention features a novel isolated Family B DNA polymerase, a Thermococcus polymerase JDF-3, and mutant recombinant forms thereof. Mutant polymerases of the invention are deficient in 3′ to 5′ exonuclease activity and/or exhibit reduced discrimination against non-conventional nucleotides relative to the wild-type form of the polymerase.
摘要翻译：本发明的特征在于新的分离的家族B DNA聚合酶，热球菌聚合酶JDF-3及其突变体重组形式。本发明的突变型聚合酶相对于野生型形式的聚合酶，缺乏3'至5'核酸外切酶活性和/或表现出对非常规核苷酸的降低的鉴别。

4. 发明授权

US08268605B2 Compositions and methods utilizing DNA polymerases 有权
标题翻译：使用DNA聚合酶的组合物和方法
公开(公告)号：US08268605B2
公开(公告)日：2012-09-18
申请号：US09896923
申请日：2001-06-29
申请人： Joseph A. Sorge , Connie Jo Hansen , Holly Hogrefe
发明人： Joseph A. Sorge , Connie Jo Hansen , Holly Hogrefe
IPC分类号： C12N9/12
CPC分类号： B82Y5/00 , B82Y10/00 , C12N9/1252 , C12N9/1276
摘要： The invention features a novel isolated Family B DNA polymerase, a Thermococcus polymerase JDF-3, and mutant recombinant forms thereof. Mutant polymerases of the invention are deficient in 3′ to 5′ exonuclease activity and/or exhibit reduced discrimination against non-conventional nucleotides relative to the wild-type form of the polymerase.
摘要翻译：本发明的特征在于新的分离的家族B DNA聚合酶，热球菌聚合酶JDF-3及其突变体重组形式。本发明的突变型聚合酶相对于野生型形式的聚合酶，缺乏3'至5'核酸外切酶活性和/或表现出对非常规核苷酸的降低的鉴别。

5. 发明申请

US20080280291A1 Compositions and methods utilizing DNA polymerases 有权
公开(公告)号：US20080280291A1
公开(公告)日：2008-11-13
申请号：US11435018
申请日：2006-05-15
申请人： Joseph A. Sorge , Connie Jo Hansen , Holly Hogrefe
发明人： Joseph A. Sorge , Connie Jo Hansen , Holly Hogrefe
IPC分类号： C12Q1/68 , C12N9/00
CPC分类号： B82Y10/00 , B82Y5/00 , C12N9/1252 , C12N9/1276
摘要： The invention features a novel isolated Family B DNA polymerase, a Thermococcus polymerase JDF-3, and mutant recombinant forms thereof. Mutant polymerases of the invention are deficient in 3′ to 5′ exonuclease activity and/or exhibit reduced discrimination against non-conventional nucleotides relative to the wild-type form of the polymerase.

6. 发明授权

US08772006B2 Compositions and methods utilizing DNA polymerases 有权
标题翻译：使用DNA聚合酶的组合物和方法
公开(公告)号：US08772006B2
公开(公告)日：2014-07-08
申请号：US11435018
申请日：2006-05-15
申请人： Joseph A. Sorge , Connie Jo Hansen , Holly Hogrefe
发明人： Joseph A. Sorge , Connie Jo Hansen , Holly Hogrefe
IPC分类号： C12N9/12 , C12P19/34
CPC分类号： B82Y10/00 , B82Y5/00 , C12N9/1252 , C12N9/1276
摘要： The invention features a novel isolated Family B DNA polymerase, a Thermococcus polymerase JDF-3, and mutant recombinant forms thereof. Mutant polymerases of the invention are deficient in 3′ to 5′ exonuclease activity and/or exhibit reduced discrimination against non-conventional nucleotides relative to the wild-type form of the polymerase.
摘要翻译：本发明的特征在于新的分离的家族B DNA聚合酶，热球菌聚合酶JDF-3及其突变体重组形式。本发明的突变型聚合酶相对于野生型形式的聚合酶，缺乏3'至5'核酸外切酶活性和/或表现出对非常规核苷酸的降低的鉴别。

7. 发明授权

US06803201B2 Compositions and methods for polynucleotide sequence determination 失效
标题翻译：用于多核苷酸序列测定的组合物和方法
公开(公告)号：US06803201B2
公开(公告)日：2004-10-12
申请号：US10056598
申请日：2002-01-24
申请人： Joseph A. Sorge , Bahram Arezi , Holly Hogrefe
发明人： Joseph A. Sorge , Bahram Arezi , Holly Hogrefe
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q1/6818 , C12Q1/6858 , C12Q2535/125 , C12Q2525/161 , C12Q2565/101
摘要： The present invention relates to a method for identifying a nucleotide at a predetermined location on a target polynucleotide. The method involves single nucleotide extension reaction comprising an oligonucleotide primer comprising a first sequence and a second sequence or a tag. The method may further comprises a probe which hybridizes to the second sequence or an anti-tag molecule which interacts with the tag, where the hybridization or interaction causes a detectable signal transfer which is indicative of the identity of the nucleotide base at the predetermined location. The invention further provides compositions and kits for performing the subject method of the invention.
摘要翻译：本发明涉及鉴定靶多核苷酸上预定位置的核苷酸的方法。该方法涉及包含含有第一序列和第二序列或标签的寡核苷酸引物的单核苷酸延伸反应。该方法还可以包括与第二序列杂交的探针或与标签相互作用的抗标签分子，其中杂交或相互作用引起可检测的信号转移，其指示在预定位置处的核苷酸碱基的身份。本发明还提供了用于实施本发明的主题方法的组合物和试剂盒。

8. 发明授权

US08383343B2 Methods of enriching for and identifying polymorphisms 有权
标题翻译：富集和鉴定多态性的方法
公开(公告)号：US08383343B2
公开(公告)日：2013-02-26
申请号：US10391090
申请日：2003-03-18
申请人： Joseph A. Sorge
发明人： Joseph A. Sorge
IPC分类号： C12Q1/68
CPC分类号： C12N15/1072 , C12Q1/6827 , C12Q1/6869 , C12Q2565/519 , C12Q2565/531 , C12Q2533/101 , C12Q2522/101
摘要： The invention encompasses methods for enriching for and identifying a polymorphism within a nucleic acid sample either by separating a subset of a nucleic acid sample or by selectively replicating a subset of a nucleic acid sample such that the polymorphism is contained within a nucleic acid population with reduced complexity, and then identifying the polymorphism within the enriched nucleic acid sample. Methods also are disclosed for enriching for and identifying a polymorphism by contacting a nucleic acid sample that includes a subset of nucleic acid molecules having a sequence that binds to a sequence-specific binding activity with a molecule having a sequence-specific binding activity under conditions which permit specific binding, such that the subset of nucleic acid molecules bound to the activity is enriched for nucleic acid molecules having the sequence recognized by the sequence-specific binding activity, and detecting a polymorphism with respect to a reference sequence in the subset of nucleic acid molecules.
摘要翻译：本发明包括用于通过分离核酸样品的子集或通过选择性复制核酸样品的子集来使得核酸样品中的多态性富集和鉴定核酸样品中的多态性的方法，使得多态性包含在具有降低的核酸群体的核酸群体内复杂性，然后鉴定富集的核酸样品内的多态性。公开了通过使包含具有与序列特异性结合活性结合的序列的核酸分子的子集与具有序列特异性结合活性的分子的核酸样品接触的方法来丰富和鉴定多态性的方法，允许特异性结合，使得与活性结合的核酸分子的子集富含具有由序列特异性结合活性识别的序列的核酸分子，并且检测核酸子集中的参考序列的多态性分子。

9. 发明授权

US07858359B2 Method for tapping the immunological repertoire 失效
标题翻译：攻击免疫学曲目的方法
公开(公告)号：US07858359B2
公开(公告)日：2010-12-28
申请号：US11558384
申请日：2006-11-09
申请人： William D. Huse , Gregory P. Winter , Lutz Riechmann , Joseph A. Sorge , Richard A. Lerner
发明人： William D. Huse , Gregory P. Winter , Lutz Riechmann , Joseph A. Sorge , Richard A. Lerner
IPC分类号： C12N1/21
CPC分类号： C07K16/00 , A61K38/00 , C07K16/468 , C07K2317/56
摘要： The present invention relates to a method for isolating from the immunological gene repertoire a gene coding for a receptor having the ability to bind a preselected ligand. Receptors produced by the gene isolated by the method, particularly catalytic receptors, are also contemplated.
摘要翻译：本发明涉及从免疫基因库中分离编码具有结合预选配体的能力的受体的基因的方法。还考虑了通过该方法分离的基因产生的受体，特别是催化受体。

10. 发明申请

US20100248246A1 METHODS OF ENRICHING FOR AND IDENTIFYING POLYMORPHISMS 审中-公开
标题翻译：增强和鉴别多态性的方法
公开(公告)号：US20100248246A1
公开(公告)日：2010-09-30
申请号：US12749070
申请日：2010-03-29
申请人： Joseph A. Sorge , Ronald W. Davis
发明人： Joseph A. Sorge , Ronald W. Davis
IPC分类号： C12Q1/68
CPC分类号： C12N15/1072 , C12Q1/6827 , C12Q1/6869 , C12Q2565/519 , C12Q2565/531 , C12Q2533/101 , C12Q2522/101
摘要： The invention encompasses methods for enriching for and identifying a polymorphism within a nucleic acid sample either by separating a subset of a nucleic acid sample or by selectively replicating a subset of a nucleic acid sample such that the polymorphism is contained within a nucleic acid population with reduced complexity, and then identifying the polymorphism within the enriched nucleic acid sample. Methods also are disclosed for enriching for and identifying a polymorphism by contacting a nucleic acid sample that includes a subset of nucleic acid molecules having a sequence that binds to a sequence-specific binding activity with a molecule having a sequence-specific binding activity under conditions which permit specific binding, such that the subset of nucleic acid molecules bound to the activity is enriched for nucleic acid molecules having the sequence recognized by the sequence-specific binding activity, and detecting a polymorphism with respect to a reference sequence in the subset of nucleic acid molecules.
摘要翻译：本发明包括用于通过分离核酸样品的子集或通过选择性复制核酸样品的子集来使得核酸样品中的多态性富集和鉴定核酸样品中的多态性的方法，使得多态性包含在具有降低的核酸群体的核酸群体内复杂性，然后鉴定富集的核酸样品内的多态性。公开了通过使包含具有与序列特异性结合活性结合的序列的核酸分子的子集与具有序列特异性结合活性的分子的核酸样品接触的方法来丰富和鉴定多态性的方法，允许特异性结合，使得与活性结合的核酸分子的子集富含具有由序列特异性结合活性识别的序列的核酸分子，并且检测核酸子集中的参考序列的多态性分子。

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