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1. 发明授权

US4556634A High sensitivity assay method 失效
标题翻译：高灵敏度测定方法
公开(公告)号：US4556634A
公开(公告)日：1985-12-03
申请号：US506844
申请日：1983-06-22
申请人： Hideo Misaki , Masahiko Taniuchi
发明人： Hideo Misaki , Masahiko Taniuchi
IPC分类号： C12Q1/00 , C12Q1/26 , C12Q1/32
CPC分类号： C12Q1/008
摘要： A high sensitivity assay method of the coenzyme cyclic assay type, eliminates the need for a separate indication reaction, by using the cycling reaction itself as the indication reaction, according to the following steps: ##STR1## the thus-formed formazan is then colorimetrically measured, to give a highly sensitive determination of the quantity of the substance to be assayed, e.g. NAD.sup.+, NADP.sup.+, malic acid or .gamma.-aminobutyric acid in human serum.
摘要翻译：根据以下步骤，通过使用循环反应本身作为指示反应，辅酶循环测定型的高灵敏度测定方法不需要单独的指示反应：（1）主反应体系：其中AH2 或A：要测定的物质; NAD（P）+：NAD +或NADP +; NAD（P）H：还原形式的NAD +或NADP +; （2）分解反应：中和：碱中和：其中M：碱金属和R：低级烷基; （3）循环反应：然后比色测定如此形成的甲，，以测定待测物质的量的高灵敏度。 NAD +，NADP +，苹果酸或γ-氨基丁酸。

2. 发明授权

US5633143A Method for the quantitative determination of D-3-hydroxybutyric acid and acetoacetic acid, and analytical reagent therefor 失效
标题翻译：定量测定D-3-羟基丁酸和乙酰乙酸的方法及其分析试剂
公开(公告)号：US5633143A
公开(公告)日：1997-05-27
申请号：US244450
申请日：1994-05-26
申请人： Shigeru Ueda , Hideo Misaki , Shigeru Ikuta , Mamoru Takahashi
发明人： Shigeru Ueda , Hideo Misaki , Shigeru Ikuta , Mamoru Takahashi
IPC分类号： C12Q1/32 , C12Q1/52 , G01N31/00 , G01N33/53
CPC分类号： C12Q1/32 , Y10S435/966 , Y10S435/973 , Y10T436/104998
摘要： Disclosed is a method for the quantitative determination of D-3-hydroxybutyric acid and acetoacetic acid, which comprises reacting a biological sample containing D-3-hydroxybutyric acid and acetoacetic acid, with a reagent comprising: (1) a D-3-hydroxybutyrate dehydrogenase, (2) A.sub.1 and (3) B.sub.1, the components (1), (2) and (3) participating in the following cycling reaction: ##STR1## thereby effecting the enzymatic cycling reaction, and measuring a change in the amount of A.sub.2 formed or the amount of B.sub.1 consumed. Also disclosed is an analytical reagent comprising the components (1), (2) and (3) for use in the above method. The method and the analytical reagent ensure rapidness and accuracy in the determination of D-3-hydroxybutyric acid and acetoacetic acid, even with the use of a small quantity of a biological sample, so that they are very useful in application fields, such as clinical diagnosis and food testing.
摘要翻译： PCT No.PCT / JP91 / 01706 Sec。 371日期：1994年5月26日 102（e）日期1994年5月26日PCT 1991年12月12日PCT PCT。公开号WO93 / 12254 日期：1993年6月24日公开是定量测定D-3-羟基丁酸和乙酰乙酸的方法，其包括使含有D-3-羟基丁酸和乙酰乙酸的生物样品与包含以下物质的试剂反应：（1） D-3-羟基丁酸脱氢酶，（2）A1和（3）B1，参与以下循环反应的组分（1），（2）和（3）：进行酶循环反应，并测量形成的A2的量的变化或消耗的B1的量。还公开了包含用于上述方法的组分（1），（2）和（3）的分析试剂。该方法和分析试剂确保即使使用少量生物样品也能快速，准确地测定D-3-羟基丁酸和乙酰乙酸，因此它们在临床应用领域非常有用诊断和食品检测。

3. 发明授权

US5162201A Analytical method making use of monoglyceride lipase 失效
标题翻译：使用单甘油脂肪酶的分析方法
公开(公告)号：US5162201A
公开(公告)日：1992-11-10
申请号：US668169
申请日：1991-03-12
申请人： Shigeyuki Imamura , Mamoru Takahasi , Hideo Misaki , Kazuo Matsuura
发明人： Shigeyuki Imamura , Mamoru Takahasi , Hideo Misaki , Kazuo Matsuura
IPC分类号： C12N9/18 , C12Q1/44
CPC分类号： C12Q1/44 , C12N9/18
摘要： Disclosed herein is a novel monoglygeride lipase at least capable of catalyzing an enzymatic reaction of the following equation (a) and as substrate specificity, capable of acting on monoglyceride but incapable of acting on diglyceride and triglyceride:Monoglyceride+H.sub.2 O.fwdarw.Glycerol+Fatty acid (a)The monoglyceride lipase is produced by culturing a specific monoglyceride lipase producing microorganism of Bacillus and then collecting the monoglyceride lipase from the resulting culture. A method is also disclosed for the analysis of a monoglyceride-containing sample solution. The monoglyceride lipase is caused to act on the sample solution upon measurement of the monoglyceride in the sample solution. Either one of glycerol and the fatty acid formed in accordance with the equation (a) is then measured.
摘要翻译：本文公开了一种新颖的单甙脂肪酶，其至少能够催化以下等式（a）的酶反应和作为底物特异性，其能够作用于甘油单酯但不能作用于甘油二酯和甘油三酸酯：单甘油酯+ H 2 O→甘油+脂肪酸（a）单酸甘油酯脂肪酶是通过培养产生芽孢杆菌的特定单酸甘油酯脂肪酶微生物，然后从得到的培养物中收集单甘油脂肪酶产生的。还公开了用于分析含单甘油酯的样品溶液的方法。当测量样品溶液中的单甘油酯时，使甘油单酯脂肪酶作用于样品溶液。然后测量根据方程（a）形成的甘油和脂肪酸中的任一种。

4. 发明授权

US4496655A Process for the production of tyramine oxidase 失效
标题翻译：酪胺氧化酶生产工艺
公开(公告)号：US4496655A
公开(公告)日：1985-01-29
申请号：US454060
申请日：1982-12-28
申请人： Eiichi Yoshino , Kazuo Matsuura , Hideo Misaki
发明人： Eiichi Yoshino , Kazuo Matsuura , Hideo Misaki
IPC分类号： C12N9/06 , C12R1/06 , C12Q1/26 , C12Q1/36
CPC分类号： C12N9/0022 , Y10S435/83
摘要： Tyramine oxidase, which is useful for the clinical diagnosis of leucine aminopeptidase activity in serum, is produced by culturing the microorganism Arthrobacter sp. B-0813 FERM-P No. 6240 in a nutrient medium, and isolating thus-produced tyramine oxidase from the cultured medium.
摘要翻译：酪氨酸氧化酶可用于血清中亮氨酸氨基肽酶活性的临床诊断，是通过培养微生物节杆菌属（Arthrobacter sp。 B-0813 FERM-P No.6240在营养培养基中，并从培养基中分离由此产生的酪胺氧化酶。

5. 发明授权

US4420562A Method for producing creatinase 失效
标题翻译：生成肌酸酐酶的方法
公开(公告)号：US4420562A
公开(公告)日：1983-12-13
申请号：US371458
申请日：1982-04-23
申请人： Shigeru Ikuta , Kazuo Matsuura , Hideo Misaki
发明人： Shigeru Ikuta , Kazuo Matsuura , Hideo Misaki
IPC分类号： C12N9/78 , C12N9/80 , C12R1/07
CPC分类号： C12R1/07 , C12N9/78 , Y10S435/815 , Y10S435/832
摘要： Isolation method of creatinase is disclosed. The method comprises culturing a microorganism belonging to Bacillus, for example, B-0618 strain (deposition No. FERM-P 4049 at Fermentation Research Institute, Agency of Industrial Science and Technology, Japan) to obtain cells from the cultured product, obtaining sarcosine oxidase and creatinase-containing solution, fractionally eluting sarcosine oxidase and creatinase by anion exchange chromatography to obtain a creatinase fraction and then collecting creatinase.
摘要翻译：公开了肌酸酐酶的分离方法。该方法包括培养属于芽孢杆菌的微生物，例如B-0618菌株（日本工业科学技术学院发酵研究所的淀粉编号FERM-P4049），从培养物中获得细胞，得到肌氨酸氧化酶和含肌酸酐溶液，通过阴离子交换色谱法分级洗脱肌氨酸氧化酶和肌酸酐酶，得到肌酸酐酶级分，然后收集肌酸酐酶。

6. 发明授权

US4347323A Glycerol kinase from Streptomyces canus 失效
标题翻译：来自链霉菌属的甘油激酶
公开(公告)号：US4347323A
公开(公告)日：1982-08-31
申请号：US154029
申请日：1980-05-28
申请人： Shigeyuki Imamura , Tohru Matsumoto , Naoki Muto , Hideo Misaki
发明人： Shigeyuki Imamura , Tohru Matsumoto , Naoki Muto , Hideo Misaki
IPC分类号： C12N9/12 , C12R1/465 , C12Q1/48
CPC分类号： C12N9/12 , Y10S435/886
摘要： A novel glycerol kinase is produced from the microorganism Streptomyces canus FERM-P No. 4977 and is useful as a diagnostic reagent for assay of triglycerides and glycerol in body fluids such as serum.
摘要翻译：从微生物链霉菌FERM-P No.4977生产新型甘油激酶，可用作体液如血清中甘油三酯和甘油的诊断试剂。

7. 发明授权

US5286627A Method of high-sensitive analysis of bile acid and reagent composition for the analysis 失效
标题翻译：胆汁酸敏感性分析方法及试剂组成分析
公开(公告)号：US5286627A
公开(公告)日：1994-02-15
申请号：US510716
申请日：1990-04-18
申请人： Shigeru Ueda , Masashi Tanno , Hideo Misaki
发明人： Shigeru Ueda , Masashi Tanno , Hideo Misaki
IPC分类号： C12Q1/32 , C12Q1/60 , C12N9/02 , C12N9/08 , C12Q1/26
CPC分类号： C12Q1/32 , Y10S435/81
摘要： A method for a high-sensitivity quantitative analysis of bile acid in a bile acid-containing sample utilizes a reagent comprising:(1) a steroid dehydrogenase which is capable of effecting a reversible reaction producing oxobile acid using bile acid, as a substrate, and a nicotinamide adenine dinucleotide phosphate compound (hereinafter referred to as an NADP compound) or a nicotinamide adenine dinucleotide compound (hereinafter referred to as an NAD compound) as coenzyme;(2) a compound A.sub.1 selected from the group consisting of NADP compounds and NAD compounds, in an amount surplus relative to the amount of bile acid;(3)(i) compound B.sub.1 selected from the group consisting of a reduced NAD compound and a reduced NADP compound, compound B.sub.1 being a reduced NAD compound, when A.sub.1 is an NADP compound, and a reduced NADP compound, when A.sub.1 is an NAD compound, or(ii) a compound B.sub.2 which is an oxidized product of B.sub.1, or(iii) a mixture of B.sub.1 and B.sub.2, where the amount of B.sub.1 plus B.sub.2 is less than 1/100 of the molar amount of A.sub.1, and(iv) a second dehydrogenase which does not react with bile acid and compound A.sub.1, but does effect a reaction converting compound B.sub.2 into compound B.sub.1, and the substrate of the second dehydrogenase.
摘要翻译：用于胆汁酸样品中胆汁酸的高灵敏度定量分析的方法使用试剂，其包括：（1）能够实现使用胆汁酸作为底物产生氧化酸的可逆反应的类固醇脱氢酶，以及烟酰胺腺嘌呤二核苷酸磷酸化合物（以下称为NADP化合物）或烟酰胺腺嘌呤二核苷酸化合物（以下称为NAD化合物）作为辅酶; （2）选自NADP化合物和NAD化合物的化合物A1，相对于胆汁酸量为过量; （3）（A1）当NAD为NAD的化合物时，当A1为NADP化合物时，化合物B1为还原的NAD化合物，还原的NADP化合物为化合物B1，还原的NADP化合物为NAD 化合物，或（ii）作为B1的氧化物的化合物B2或（iii）B1和B2的混合物，其中B1加B2的量小于A1摩尔量的1/100，和（ iv）不与胆汁酸和化合物A1反应的第二脱氢酶，但是将化合物B2转化为化合物B1和将第二脱氢酶的底物进行反应。

8. 发明授权

US5126246A Reagent for analysis of triglycerides and analysis using the same 失效
标题翻译：用于分析甘油三酸酯的试剂和使用其分析
公开(公告)号：US5126246A
公开(公告)日：1992-06-30
申请号：US435003
申请日：1989-11-09
申请人： Shigeyuki Imamura , Mamoru Takahashi , Hideo Misaki , Kazuo Matsuura
发明人： Shigeyuki Imamura , Mamoru Takahashi , Hideo Misaki , Kazuo Matsuura
IPC分类号： G01N33/92 , C12Q1/44 , C12Q1/48 , C12Q1/61
CPC分类号： C12Q1/48 , C12Q1/44 , Y10S435/81 , Y10S435/832 , Y10S436/815 , Y10S530/825 , Y10S530/83
摘要： Reagent for analysis of triglycerides contained in blood serum is provided, which comprises lipases and monoglyceride lipases capable of acting on monoglycerides having substrate specificity and capable of catalyzing the following enzymatic reaction: monoglyceride+H.sub.2 O.fwdarw.glycerol+fatty acids. The glycerol or fatty acids are measured to learn an amount of the triglycerides or fatty acid by any known analytical method.
摘要翻译：提供了用于分析血清中含有的甘油三酯的试剂，其包含能够作用于具有底物特异性并能够催化以下酶促反应的单酸甘油酯的脂肪酶和甘油单酯脂肪酶：单酸甘油酯+ H 2 O->甘油+脂肪酸。测量甘油或脂肪酸，以通过任何已知的分析方法学习一定量的甘油三酯或脂肪酸。

9. 发明授权

US4921786A Novel NAD synthetase, assay method using said novel NAD synthetase and a process for production thereof 失效
标题翻译：新型NAD合成酶，使用所述新型NAD合成酶的测定方法及其生产方法
公开(公告)号：US4921786A
公开(公告)日：1990-05-01
申请号：US94813
申请日：1987-09-10
申请人： Mamoru Takahashi , Hideo Misaki , Shigeyuki Imamura , Kazuo Matsuura
发明人： Mamoru Takahashi , Hideo Misaki , Shigeyuki Imamura , Kazuo Matsuura
IPC分类号： C12N9/00 , C12P1/00 , C12P3/00
CPC分类号： C12N9/93 , C12P1/00 , C12P3/00 , C12R1/07
摘要： A novel NAD synthetase is produced by culturing a broth of Bacillus stearothermophilus H-804 FERM BP-1408. This new enzyme selectively catalyzes the reaction ##STR1## without catalyzing the reaction ##STR2## The enzyme uses ammonia or ammonium ion as a substrate, but does not use either glutamine or asparagine. Also disclosed is an assay method using the enzyme, for any one of ATP, deamide-NAD, ammonia or ammonium ion in a specimen to be assayed.

10. 发明授权

US4791057A Highly sensitive enzymatic assay method 失效
标题翻译：高灵敏度酶法测定
公开(公告)号：US4791057A
公开(公告)日：1988-12-13
申请号：US757953
申请日：1985-07-23
申请人： Hideo Misaki , Shigeru Ueda
发明人： Hideo Misaki , Shigeru Ueda
IPC分类号： G01N33/50 , C12Q1/26 , C12Q1/28 , C12Q1/32 , G01N33/74 , G01N33/92
CPC分类号： G01N33/92 , C12Q1/26 , C12Q1/32 , G01N33/743 , Y10S436/817
摘要： A highly sensitive quantitative assay method for a component which is 3.beta.-hydroxysteroid or 3-ketosteroid, in a specimen to be assayed, comprising causing this component in the specimen to take part in the cycling reaction ##STR1## and measuring a detectable change in the reaction system. There is thus provided a 3.beta.-hydroxysteroid - 3-ketosteroid cycling reaction using 3.beta.-hydroxysteroid oxidase, which consumes O.sub.2 and generates H.sub.2 O.sub.2 and 3-ketosteroid, with a substrate of 3.beta.-hydroxysteroid, or 3.beta.-hydroxysteroid dehydrogenase, which consumes reduced NAD(P) and generate NAD(P) and 3.beta.-hydroxysteroid, with a substrate of 3-ketosteroid. Example of specimens are specimens which contain 3-hydroxysteroid or 3-ketosteroid, or which liberate or generate such a component. By proceeding at a rate of more than ten cycles per minute and measuring the amount of a detectable change in the reaction, the component in a specimen can easily and sensitively be detected with good accuracy.
摘要翻译：在待测样品中的3β-羟基类固醇或3-酮甾类成分的高度灵敏的定量测定方法，包括使样品中的该组分参与循环反应＆IMAGE>并测量可检测的变化反应体系。因此，提供了使用3种β-羟基类固醇氧化酶的3β-羟基甾类-3-酮类固醇循环反应，其消耗O 2并产生H 2 O 2和3-酮甾类，具有3β-羟基类固醇或3β-羟基类固醇脱氢酶的底物，其中消耗NAD（P）并产生NAD（P）和3β-羟基类固醇，与3-酮类固醇的底物。样品的实例是含有3-羟基类固醇或3-酮甾类的样品，或释放或产生这样的组分。通过以每分钟超过10个循环的速率进行测量并测量反应中可检测的变化量，可以以高精度容易且灵敏地检测样品中的组分。

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