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1. 发明授权

US4135980A Choline oxidase 失效
标题翻译：胆固醇氧化酶
公开(公告)号：US4135980A
公开(公告)日：1979-01-23
申请号：US789990
申请日：1977-04-22
申请人： Shigeru Ikuta , Yoshifumi Horiuchi , Hideo Misaki , Kazuo Matsuura , Shigeyuki Imamura , Naoki Muto
发明人： Shigeru Ikuta , Yoshifumi Horiuchi , Hideo Misaki , Kazuo Matsuura , Shigeyuki Imamura , Naoki Muto
IPC分类号： C12N9/04 , C12N9/16 , C12Q1/32 , C12Q1/44 , C12Q1/46 , C12D13/10 , G01N31/14
CPC分类号： C12N9/0006 , C12N9/16 , C12Q1/32 , C12Q1/44 , C12Q1/46 , C12R1/06 , C12R1/465 , Y10S435/83
摘要： A novel enzyme choline oxidase is produced by culturing a microorganism belonging to the genus Arthrobacter, and particularly the species Arthrobacter globiformis B-0577 FERM-P No. 3518, NRRL B-11097, and isolating the choline oxidase thus produced from the culture medium. Choline oxidase is useful for the determination of choline or betaine aldehyde in a sample by mixing the new product with the sample and then measuring the generated hydrogen peroxide, betaine or consumed oxygen.

2. 发明授权

US4385112A Nucleoside oxidase and process for making same, and process and kit for using same 失效
标题翻译：核苷氧化酶及其制备方法，及其使用方法和试剂盒
公开(公告)号：US4385112A
公开(公告)日：1983-05-24
申请号：US292154
申请日：1981-08-12
申请人： Hideo Misaki , Shigeru Ikuta , Kazuo Matsuura
发明人： Hideo Misaki , Shigeru Ikuta , Kazuo Matsuura
IPC分类号： C07H19/04 , C07H21/00 , C12N9/04 , C12P19/28 , C12P19/38 , C12Q1/26 , C12Q1/28 , C12N9/06 , C12P7/40 , C12P13/00 , C12Q1/34 , C12Q1/42 , C12Q1/68 , C12R1/40
CPC分类号： C07H21/00 , C07H19/04 , C12N9/0006 , C12P19/28 , C12P19/38 , C12Q1/26 , C12Q1/28 , C12Y101/03028 , Y10S435/81 , Y10S435/877
摘要： A microorganism strain B-0781 belonging to the genus Pseudomonas isolated from a soil sample from an onion field in Japan, produces a novel enzyme nucleoside oxidase having substrate specificity on various nucleosides and enzyme action to catalyze enzymatic reactions involving various nucleosides. The novel nucleoside oxidase is produced by culturing a nucleoside-oxidase-producing microorganism belonging to the genus Pseudomonas in a nutrient medium containing assimilable carbon and nitrogen and inorganic salt, and isolating the thus-formed nucleoside oxidase from the cultured cells. Various nucleoside-5'-carboxylic acids can be produced, by incubating the novel nucleoside oxidase with a nucleoside having a 5'-hydroxymethyl group, in an aqueous medium under aerobic conditions, and isolating the thus-formed nucleoside-5'-carboxylic acid from the incubation medium. Assay methods for nucleosides in liquid samples are provided, which comprise incubating the sample with nucleoside oxidase, thereby consuming oxygen and generating hydrogen peroxide and nucleoside-5'-carboxylic acid by acting on nucleoside, and quantitatively determining consumed oxygen or liberated hydrogen peroxide. A kit for nucleoside assay is provided, which contains the recited ingredients.
摘要翻译：属于从日本洋葱田土壤样品中分离出的假单胞菌属的微生物菌株B-0781产生了对各种核苷具有底物特异性和酶作用的新的酶核苷氧化酶，以催化涉及各种核苷的酶反应。通过在含有可同化碳，氮和无机盐的营养培养基中培养属于假单胞菌属的核苷 - 氧化酶产生微生物，并从培养细胞中分离由此形成的核苷氧化酶，从而产生新的核苷氧化酶。通过在有氧条件下在含水介质中孵育新的核苷氧化酶与具有5'-羟甲基的核苷，可以制备各种核苷-5'-羧酸，并分离由此形成的核苷-5'-羧酸从培养基中。提供液体样品中核苷的测定方法，其包括将样品与核苷氧化酶孵育，由此消耗氧并通过作用于核苷产生过氧化氢和核苷-5'-羧酸，并定量测定消耗的氧气或释放的过氧化氢。提供了用于核苷测定的试剂盒，其包含所述的成分。

3. 发明授权

US4346173A Process for the production of acyl-Coenzyme A oxidase 失效
标题翻译：制备酰基辅酶A氧化酶的方法
公开(公告)号：US4346173A
公开(公告)日：1982-08-24
申请号：US201123
申请日：1980-10-27
申请人： Shigeru Ikuta , Shigeyuki Imamura , Hidehiko Ishikawa , Kazuo Matsuura , Masaki Takada , Hideo Misaki
发明人： Shigeru Ikuta , Shigeyuki Imamura , Hidehiko Ishikawa , Kazuo Matsuura , Masaki Takada , Hideo Misaki
IPC分类号： C12N9/02
CPC分类号： C12Y103/03006 , C12N9/001 , C12R1/06 , C12R1/645 , C12R1/66 , C12R1/865 , Y10S435/83 , Y10S435/911 , Y10S435/913 , Y10S435/942
摘要： A process for the production of acyl-Coenzyme A oxidase, comprises culturing an acyl-Coenzyme A-oxidase-producing microorganism belonging to genus Macrophomina, genus Cladosporium, genus Aspergillus, genus Monascus, genus Saccharomyces or genus Arthrobacter in a nutrient medium, and isolating the thus-formed acyl-CoA oxidase therefrom. The preferred species of microorganism are Macrophomina phaseoli ATCC 14383, Cladosporium resinae IFO 6367, Aspergillus candidus M-4815 FERM-P No. 5226, Monascus sp. M-4800 FERM-P No. 5225, Saccharomyces cerevisiae Y 0036 FERM-P No. 5174, and Arthrobacter sp. B-720 FERM-P No. 5224, respectively.
摘要翻译：一种制备酰基辅酶A氧化酶的方法，包括在营养培养基中培养属于巨噬细胞，枝孢霉属，曲霉属，红曲霉属，酿酒酵母属或节杆菌属的酰基辅酶A氧化酶微生物，由此形成的酰基辅酶A氧化酶。微生物的优选种类是Macrophomina phaseoli ATCC 14383，Cladosporium resinae IFO 6367，Aspergillus candidus M-4815 FERM-P No.5226，Monascus sp。 M-4800 FERM-P No.5225，酿酒酵母Y 0036 FERM-P No.5174和节杆菌属（Arthrobacter sp。 B-720 FERM-P No. 5224。

4. 发明授权

US4420562A Method for producing creatinase 失效
标题翻译：生成肌酸酐酶的方法
公开(公告)号：US4420562A
公开(公告)日：1983-12-13
申请号：US371458
申请日：1982-04-23
申请人： Shigeru Ikuta , Kazuo Matsuura , Hideo Misaki
发明人： Shigeru Ikuta , Kazuo Matsuura , Hideo Misaki
IPC分类号： C12N9/78 , C12N9/80 , C12R1/07
CPC分类号： C12R1/07 , C12N9/78 , Y10S435/815 , Y10S435/832
摘要： Isolation method of creatinase is disclosed. The method comprises culturing a microorganism belonging to Bacillus, for example, B-0618 strain (deposition No. FERM-P 4049 at Fermentation Research Institute, Agency of Industrial Science and Technology, Japan) to obtain cells from the cultured product, obtaining sarcosine oxidase and creatinase-containing solution, fractionally eluting sarcosine oxidase and creatinase by anion exchange chromatography to obtain a creatinase fraction and then collecting creatinase.
摘要翻译：公开了肌酸酐酶的分离方法。该方法包括培养属于芽孢杆菌的微生物，例如B-0618菌株（日本工业科学技术学院发酵研究所的淀粉编号FERM-P4049），从培养物中获得细胞，得到肌氨酸氧化酶和含肌酸酐溶液，通过阴离子交换色谱法分级洗脱肌氨酸氧化酶和肌酸酐酶，得到肌酸酐酶级分，然后收集肌酸酐酶。

5. 发明授权

US5780256A Method and composition for quantitative determination of ammonia, .alpha.-amino acid, or .alpha.-keto acid 失效
标题翻译：用于定量测定氨，α-氨基酸或α-酮酸的方法和组合物
公开(公告)号：US5780256A
公开(公告)日：1998-07-14
申请号：US108736
申请日：1995-03-13
申请人： Shigeru Ueda , Mamoru Takahashi , Hideo Misaki , Shigeru Ikuta
发明人： Shigeru Ueda , Mamoru Takahashi , Hideo Misaki , Shigeru Ikuta
IPC分类号： C12Q1/32
CPC分类号： C12Q1/32
摘要： The present invention is directed to a method for the quantitative determination of ammonia, an .alpha.-amino acid and an .alpha.-keto acid corresponding to the .alpha.-amino acid, or a chemical substance producing any one of these compounds. The present invention is also directed to an analytical composition for use in the above method. The method of the present invention ensures rapidness and accuracy in the determination of ammonia, .alpha.-amino acids or .alpha.-keto acids, even with the use of a small quantity of a biological sample. This method is very useful in application fields, such as clinical diagnosis and food testing.
摘要翻译： PCT No.PCT / JP91 / 01785 Sec。 371日期1995年3月13日 102（e）1995年3月13日PCT PCT 1991年12月27日PCT公布。 WO92 / 15705 PCT公开号日期1992年9月17日本发明涉及一种定量测定对应于α-氨基酸的氨，α-氨基酸和α-酮酸的方法，或者是产生这些化合物中的任何一种的化学物质。本发明还涉及用于上述方法的分析组合物。本发明的方法即使使用少量的生物样品也能确保氨，α-氨基酸或α-酮酸的测定的快速和准确性。该方法在临床诊断和食品检测等应用领域非常有用。

6. 发明授权

US5633143A Method for the quantitative determination of D-3-hydroxybutyric acid and acetoacetic acid, and analytical reagent therefor 失效
标题翻译：定量测定D-3-羟基丁酸和乙酰乙酸的方法及其分析试剂
公开(公告)号：US5633143A
公开(公告)日：1997-05-27
申请号：US244450
申请日：1994-05-26
申请人： Shigeru Ueda , Hideo Misaki , Shigeru Ikuta , Mamoru Takahashi
发明人： Shigeru Ueda , Hideo Misaki , Shigeru Ikuta , Mamoru Takahashi
IPC分类号： C12Q1/32 , C12Q1/52 , G01N31/00 , G01N33/53
CPC分类号： C12Q1/32 , Y10S435/966 , Y10S435/973 , Y10T436/104998
摘要： Disclosed is a method for the quantitative determination of D-3-hydroxybutyric acid and acetoacetic acid, which comprises reacting a biological sample containing D-3-hydroxybutyric acid and acetoacetic acid, with a reagent comprising: (1) a D-3-hydroxybutyrate dehydrogenase, (2) A.sub.1 and (3) B.sub.1, the components (1), (2) and (3) participating in the following cycling reaction: ##STR1## thereby effecting the enzymatic cycling reaction, and measuring a change in the amount of A.sub.2 formed or the amount of B.sub.1 consumed. Also disclosed is an analytical reagent comprising the components (1), (2) and (3) for use in the above method. The method and the analytical reagent ensure rapidness and accuracy in the determination of D-3-hydroxybutyric acid and acetoacetic acid, even with the use of a small quantity of a biological sample, so that they are very useful in application fields, such as clinical diagnosis and food testing.
摘要翻译： PCT No.PCT / JP91 / 01706 Sec。 371日期：1994年5月26日 102（e）日期1994年5月26日PCT 1991年12月12日PCT PCT。公开号WO93 / 12254 日期：1993年6月24日公开是定量测定D-3-羟基丁酸和乙酰乙酸的方法，其包括使含有D-3-羟基丁酸和乙酰乙酸的生物样品与包含以下物质的试剂反应：（1） D-3-羟基丁酸脱氢酶，（2）A1和（3）B1，参与以下循环反应的组分（1），（2）和（3）：进行酶循环反应，并测量形成的A2的量的变化或消耗的B1的量。还公开了包含用于上述方法的组分（1），（2）和（3）的分析试剂。该方法和分析试剂确保即使使用少量生物样品也能快速，准确地测定D-3-羟基丁酸和乙酰乙酸，因此它们在临床应用领域非常有用诊断和食品检测。

7. 发明授权

US4216292A Process for the production of sarcosine oxidase 失效
标题翻译：生产肌氨酸氧化酶的方法
公开(公告)号：US4216292A
公开(公告)日：1980-08-05
申请号：US946402
申请日：1978-09-27
申请人： Shigeru Ikuta , Kazuo Matsuura , Yoshifumi Horiuchi
发明人： Shigeru Ikuta , Kazuo Matsuura , Yoshifumi Horiuchi
IPC分类号： C12N9/06 , C12Q1/26 , C12R1/07 , C12D13/10
CPC分类号： C12N9/0032 , C12Q1/26 , C12R1/07 , Y10S435/832
摘要： An enzyme sarcosine oxidase is produced by culturing a microorganism belonging to the genus Bacillus, and particularly the species Bacillus sp. B-0618, FERM-P No. 4049, NRRL No. B-11380, and isolating the sarcosine oxidase thus produced from the culture medium. Sarcosine oxidase is useful for the determination of creatinine in the presence of creatinase and creatininase in a sample by mixing the enzymes with the sample and then measuring the generated hydrogen peroxide, formaldehyde or glycine.
摘要翻译：酶肌氨酸氧化酶是通过培养属于芽孢杆菌属的微生物，特别是芽孢杆菌属种。 B-0618，FERM-P No.4049，NRRL No.B-11380，并从培养基中分离由此产生的肌氨酸氧化酶。肌氨酸氧化酶可用于通过将酶与样品混合，然后测量产生的过氧化氢，甲醛或甘氨酸来测定样品中肌酸酐酶和肌酸酐酶的肌酐。

8. 发明授权

US5116748A Process for the production of L-alanine dehydrogenase from 78-3 FERM BP-2517 失效
标题翻译：从78-3 FERM BP-2517生产L-苯胺脱氢酶的方法
公开(公告)号：US5116748A
公开(公告)日：1992-05-26
申请号：US413431
申请日：1989-09-26
申请人： Mamoru Takahashi , Shinobu Nagasawa , Shigeru Ikuta , Kazuo Matsuura
发明人： Mamoru Takahashi , Shinobu Nagasawa , Shigeru Ikuta , Kazuo Matsuura
IPC分类号： C12N1/20 , C12N9/06 , C12R1/01
CPC分类号： C12N9/0016 , C12R1/01
摘要： A process for the production of L-alanine dehydrogenase which comprises culturing an L-alanine dehydrogenase producing microorganism belonging to the genus Sporolactobacillus in a nutrient medium and isolating thus-produced L-alanine dehydrogenase from the cultured mass. The microorganism is for example Sporolactobacillus sp. 78-3 FERM BP-2517 and mutants and variants thereof having the ability to produce L-alanine dehydrogenase in recoverable amounts. This strain is a thermophile which cannot grow at 40.degree. C. but can grow at 45.degree. C. and 52.degree. C. The strain can assimilate glucose and produce lactic acid and acetic acid.

9. 发明授权

US5214170A Radioisotope iodine-labeled 1.alpha. (or 24R), 25-dihydroxy vitamin D.sub.3 失效
标题翻译：放射性同位素碘标记的1α（或24R），25-二羟基维生素D3衍生物
公开(公告)号：US5214170A
公开(公告)日：1993-05-25
申请号：US827151
申请日：1992-01-27
申请人： Miyuki Tanabe , Shigeru Ikuta
发明人： Miyuki Tanabe , Shigeru Ikuta
IPC分类号： C07C401/00
CPC分类号： C07C401/00 , Y02P20/55 , Y10T436/173845
摘要： A novel 1.alpha. (or 24R), 25-dihydroxy vitamin D.sub.3 amino acid derivative of the formula ##STR1## wherein R.sub.1 is OH or --O--CO--A--NH.sub.2, and R.sub.2 and R.sub.3 are each selected from the group consisting of hydrogen, OH and --O--CO--A--NH.sub.2 ; wherein one of R.sub.2 and R.sub.3 is hydrogen, one of R.sub., R.sub.2 and R.sub.3 is OH, and one of R.sub.1, R.sub.2 and R.sub.3 is --O--CO--A--NH.sub.2 ; and wherein A is C.sub.1-10 alkylene, is produced by removing a protective group for the amino group, e.g. 9-fluorenylmethyloxycarbonyl, in the presence of a base in an inert solvent. A radioisotope iodine-labeled residue is then attached to the amino group to produce a derivative useful in the assay of 1.alpha. (or 24R), 25-dihydroxy vitamin D.sub.3 in a specimen.
摘要翻译：其中R 1是OH或-O-CO-A-NH 2的式1α（或24R），25-二羟基维生素D3氨基酸衍生物，其中R 1和R 2各自选自氢，OH和-O-CO-A-NH 2; 其中R2和R3中的一个是氢，R，R2和R3之一是OH，R1，R2和R3中的一个是-O-CO-A-NH2; 并且其中A为C 1-10亚烷基，是通过除去氨基的保护基来制备的。 9-芴基甲氧基羰基，在碱的存在下在惰性溶剂中。然后将放射性同位素碘标记的残基连接到氨基上以产生可用于测定样品中1α（或24R），25-二羟基维生素D3的衍生物。

10. 发明授权

US5117018A 1.alpha. (or 24R), 25-dihydroxy vitamin D.sub.3 derivatives 失效
标题翻译： 1（ALPHA）（OR 24R），25-二羟基维生素D 3衍生物
公开(公告)号：US5117018A
公开(公告)日：1992-05-26
申请号：US417313
申请日：1989-10-05
申请人： Miyuki Tanabe , Shigeru Ikuta
发明人： Miyuki Tanabe , Shigeru Ikuta
IPC分类号： C07C401/00
CPC分类号： C07C401/00 , Y10T436/173845
摘要： A novel 1.alpha. (or 24R), 25-dihydroxy vitamin D.sub.3 amino acid derivative of the formula ##STR1## wherein R.sub.1 is OH or --O--CO--A--NH.sub.2, and R.sub.2 and R.sub.3 are each selected from the group consisting of hydrogen, OH and --O--CO--A--NH.sub.2 ; wherein one of R.sub.2 and R.sub.3 is hydrogen, one of R.sub.1, R.sub.2 and R.sub.3 is OH, and one of R.sub.1, R.sub.2 and R.sub.3 is --O--CO--A--NH.sub.2 ; and wherein A is C.sub.1-10 alkylene, is produced by removing a protective group for the amino group, e.g. 9-fluorenylmethyloxycarbonyl, in the presence of a base in an inert solvent. A radioisotope iodine-labeled residue is then attached to the amino group to produce a derivative useful in the assay of 1.alpha. (or 24R), 25-dihydroxy vitamin D.sub.3 in a specimen.

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