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1. 发明授权

US10407657B2 Valve mechanism, cell cultivation apparatus using same, and cell cultivation method 有权
公开(公告)号：US10407657B2
公开(公告)日：2019-09-10
申请号：US14425331
申请日：2012-09-05
申请人： Guangbin Zhou , Takayuki Nozaki , Shizu Matsuoka , Ryota Nakajima , Masaharu Kiyama , Kenichi Minami
发明人： Guangbin Zhou , Takayuki Nozaki , Shizu Matsuoka , Ryota Nakajima , Masaharu Kiyama , Kenichi Minami
IPC分类号： C12M1/00 , F16K7/06 , G05D7/00 , A61M39/28
摘要： The purpose of the present invention is to provide a valve mechanism which features a simple structure adapted to collectively control the supply of gas or culture medium to a plurality of cultivation containers for simultaneous cultivation and to enhance the reliability of the valve mechanism. The valve mechanism of the invention is a valve mechanism for opening and closing passages for liquid or gas. The valve mechanism includes: clip members adapted to close a plurality of tube passages, respectively; a multiple cam member for opening the clip members in sequence by making contact with the clip members; and a rotational mechanism for rotating the multiple cam member. The clip member includes: a spring for closing a clip thereof; and a clip contact portion for a cam contact portion of the multiple cam member to make contact therewith.

2. 发明申请

US20150231628A1 Biological Sample Packaging Container and Biological Sample Conveyance Method Using Same 审中-公开
标题翻译：生物样品包装容器和使用其的生物样品输送方法
公开(公告)号：US20150231628A1
公开(公告)日：2015-08-20
申请号：US14426201
申请日：2012-09-11
申请人： Takayuki Nozaki , Masaharu Kiyama , Guangbin Zhou , Shizu Matsuoka , Ryota Nakajima , Naoko Senda
发明人： Takayuki Nozaki , Masaharu Kiyama , Guangbin Zhou , Shizu Matsuoka , Ryota Nakajima , Naoko Senda
IPC分类号： B01L3/00 , B65B3/04
CPC分类号： B01L3/508 , A01N1/0242 , B01L2200/18 , B01L2300/04 , B65B3/04 , C12M45/22
摘要： The present invention provides a biological sample packaging container including: a sample storage container having a recessed part; a lid member that seals the upper surface of the sample storage container; and at least one gasket that is provided on the rear surface of the lid member or on the upper surface of the sample storage container, wherein: the sample storage container has a function of holding a biological sample therein; the lid member includes a first lid member provided with flow channel tubes that allow liquid and gas to be moved between the sample storage container and the outside, and a second lid member having no flow channel tubes; and the sample storage container is configured in such a manner that the first lid member and the second lid member can be replaced by each other.
摘要翻译：本发明提供一种生物样品包装容器，其包括：具有凹部的样品储存容器; 封闭所述样本收容容器的上表面的盖部件; 以及至少一个垫片，其设置在所述盖部件的后表面或所述样本收容容器的上表面上，其中：所述样本收纳容器具有将生物体样品保持在其中的功能; 盖构件包括：第一盖构件，其设置有允许液体和气体在样品储存容器和外部之间移动的流动通道管和不具有流动通道管的第二盖构件; 并且样本收容容器构成为能够使第一盖部件和第二盖部件相互替换。

3. 发明申请

US20150072401A1 CULTURE VESSEL AND AUTOMATED CULTURE APPARATUS 审中-公开
标题翻译：文化船和自动文化设备
公开(公告)号：US20150072401A1
公开(公告)日：2015-03-12
申请号：US14381659
申请日：2012-03-29
申请人： Takayuki Nozaki , Guangbin Zhou , Ryota Nakajima , Shizu Matsuoka , Toyoshige Kobayashi , Naoko Senda
发明人： Takayuki Nozaki , Guangbin Zhou , Ryota Nakajima , Shizu Matsuoka , Toyoshige Kobayashi , Naoko Senda
IPC分类号： C12M1/36 , C12M1/00 , C12M3/00 , C12M1/12
CPC分类号： C12M41/48 , C12M21/08 , C12M23/44 , C12M25/04 , C12M29/10 , C12M37/00
摘要： In a closed system culture vessel to be used in an automated culture apparatus, some of the ports perform both liquid supply and gas supply functions. Culture medium is thereby made to flow always in one direction. The culture vessel is also configured so that, in the complete culture medium exchange process and the effluent recovery process for analyzing culture medium components, waste liquid medium does not get mixed into fresh culture medium.
摘要翻译：在用于自动培养装置的封闭系统培养容器中，一些端口同时执行液体供应和气体供应功能。由此使培养基始终沿一个方向流动。培养容器也配置成使得在完全培养基的交换过程和用于分析培养基成分的废水回收过程中，废液介质不会混入新鲜培养基中。

4. 发明申请

US20140186941A1 CELL CULTURE VESSEL, AND CULTURE DEVICE EQUIPPED WITH SAME 有权
标题翻译：细胞培养皿和装备有其的培养装置
公开(公告)号：US20140186941A1
公开(公告)日：2014-07-03
申请号：US14124754
申请日：2012-06-05
申请人： Guangbin Zhou , Taizo Miyazaki , Ryota Nakajima , Toyoshige Kobayashi , Shizu Matsuoka
发明人： Guangbin Zhou , Taizo Miyazaki , Ryota Nakajima , Toyoshige Kobayashi , Shizu Matsuoka
IPC分类号： C12M1/00
CPC分类号： C12M47/04 , C12M23/20 , C12M25/08 , C12M33/00 , C12M35/02 , C12M41/36 , C12M47/02
摘要： Disclosed is a cell culture vessel of the present invention, which is composed of a space enclosed by a housing that supports a medium and a cell attachment part, disposed on the bottom surface of the space, that attaches and supports the cells, the cell attachment part having a cell immobilizing mechanism that guides the cells in the space to the cell attachment part to immobilize there and a cell detachment mechanism that detaches the cells attached in the cell attachment part, the cell immobilizing mechanism having a step of applying a voltage to an electrode disposed in the cell attachment part to generate a heterogeneous electric field in the space, and the cell detachment mechanism having a step of applying a voltage to an electrode disposed in the cell attachment part to induce electrolyte in the space.
摘要翻译：公开了本发明的细胞培养容器，其由由支撑培养基的壳体包围的空间和设置在空间的底面上的细胞附着部分构成，其附着并支撑细胞，细胞附着物具有细胞固定机构的部分，其将空间中的细胞引导到细胞附着部分以固定在其上，以及细胞分离机构，其分离附着在细胞附着部分中的细胞，细胞固定机构具有向电极，其设置在电池安装部中，以在该空间中产生异质电场，并且电池拆卸机构具有向设置在电池安装部中的电极施加电压以在该空间中诱导电解质的步骤。

5. 发明申请

US20150175949A1 CELL CULTURING VESSEL, AND CELL CULTURING METHOD AND AUTOMATED CELL CULTURING DEVICE USING SAME 审中-公开
标题翻译：细胞培养血管和细胞培养方法和使用相同的自动细胞培养装置
公开(公告)号：US20150175949A1
公开(公告)日：2015-06-25
申请号：US14408670
申请日：2012-06-19
申请人： Guangbin Zhou , Takayuki Nozaki , Shizu Matsuoka
发明人： Guangbin Zhou , Takayuki Nozaki , Shizu Matsuoka
IPC分类号： C12M1/00 , C12M1/36 , C12N5/074 , C12M3/00
CPC分类号： C12M23/28 , C12M23/10 , C12M23/38 , C12M23/46 , C12M25/02 , C12M41/48 , C12N5/0696
摘要： The purpose of the present invention is to decrease cell culturing vessel running cost and reduce the amount of industrial waste. A cell culturing vessel provided with a container frame and a bottom face support wherein: the cell culturing space is formed by the frame and the bottom face support; the frame is provided with a securing means for securing the bottom face support so as to be attachable and detachable; and an exchangeable culturing surface is provided on the bottom face support.
摘要翻译：本发明的目的是减少细胞培养容器的运行成本并减少工业废物的量。一种细胞培养容器，其具有容器框架和底面支撑体，其中：所述细胞培养空间由所述框架和所述底面支撑体形成; 框架设置有用于固定底面支撑件以固定和拆卸的固定装置; 并且在底面支撑件上设置可交换的培养面。

6. 发明授权

US09487747B2 Cell culture device 有权
标题翻译：细胞培养装置
公开(公告)号：US09487747B2
公开(公告)日：2016-11-08
申请号：US14124754
申请日：2012-06-05
申请人： Guangbin Zhou , Taizo Miyazaki , Ryota Nakajima , Toyoshige Kobayashi , Shizu Matsuoka
发明人： Guangbin Zhou , Taizo Miyazaki , Ryota Nakajima , Toyoshige Kobayashi , Shizu Matsuoka
IPC分类号： C12M3/00 , C12M1/00 , C12M1/42 , C12M1/12 , C12M1/26 , C12M1/34
CPC分类号： C12M47/04 , C12M23/20 , C12M25/08 , C12M33/00 , C12M35/02 , C12M41/36 , C12M47/02
摘要： Disclosed is a cell culture vessel of the present invention, which is composed of a space enclosed by a housing that supports a medium and a cell attachment part, disposed on the bottom surface of the space, that attaches and supports the cells, the cell attachment part having a cell immobilizing mechanism that guides the cells in the space to the cell attachment part to immobilize there and a cell detachment mechanism that detaches the cells attached in the cell attachment part, the cell immobilizing mechanism having a step of applying a voltage to an electrode disposed in the cell attachment part to generate a heterogeneous electric field in the space, and the cell detachment mechanism having a step of applying a voltage to an electrode disposed in the cell attachment part to induce electrolyte in the space.
摘要翻译：公开了本发明的细胞培养容器，其由由支撑培养基的壳体包围的空间和设置在空间的底面上的细胞附着部分构成，其附着并支撑细胞，细胞附着物具有细胞固定机构的部分，其将空间中的细胞引导到细胞附着部分以固定在其上，以及细胞分离机构，其分离附着在细胞附着部分中的细胞，细胞固定机构具有向电极，其设置在电池安装部中，以在该空间中产生异质电场，并且电池拆卸机构具有向设置在电池安装部中的电极施加电压以在该空间中诱导电解质的步骤。

7. 发明申请

US20140302597A1 CELL CULTURE CONTAINER, AND AUTOMATED CELL SUBCULTURE DEVICE AND CELL SUBCULTURE METHOD USING SAME 审中-公开
标题翻译：细胞培养容器和自动细胞培养装置和细胞再培养方法
公开(公告)号：US20140302597A1
公开(公告)日：2014-10-09
申请号：US14365315
申请日：2011-12-14
申请人： Guangbin Zhou , Shizu Matsuoka
发明人： Guangbin Zhou , Shizu Matsuoka
IPC分类号： C12N5/071
CPC分类号： C12N5/0602 , C12M23/04 , C12M23/34
摘要： Provided is a cell culture container that makes it possible to subculture cells without a dispensing operation using a pipette and also to prevent microbial contamination in the culture container during cell subculture. The cell culture container is provided, within a culture container (1) forming a sealable first culture zone, with a partition member (4) having a wall surface formed lower than the wall surface of the culture container and forming a second culture zone; and has means for raising and lowering the partition member while the culture container is in a sealed state. Magnets (4A, 5A, 6A) are used as the means for raising and lowering the partition member.
摘要翻译：提供了一种细胞培养容器，其可以使用移液管不经分注操作传代培养细胞，并且还可以在细胞传代培养期间防止培养容器中的微生物污染。在形成可密封的第一培养区的培养容器（1）内设置有分隔构件（4），所述分隔构件（4）的壁面形成为低于培养容器的壁面，形成第二培养区域; 并且具有用于在培养容器处于密封状态时升高和降低分隔构件的装置。磁体（4A，5A，6A）用作升降分隔构件的装置。

8. 发明授权

US5591250A Material and process for separating carbon dioxide from methane 失效
标题翻译：从甲烷分离二氧化碳的材料和工艺
公开(公告)号：US5591250A
公开(公告)日：1997-01-07
申请号：US368995
申请日：1995-01-05
申请人： S. Alexander Stern , Hiroyoshi Kawakami , Ajay Y. Houde , Guangbin Zhou
发明人： S. Alexander Stern , Hiroyoshi Kawakami , Ajay Y. Houde , Guangbin Zhou
IPC分类号： B01D53/22 , B01D71/62 , B01D71/64 , C07C7/144
CPC分类号： B01D71/64 , B01D53/228 , B01D71/62 , C07C7/144 , B01D2257/504 , Y02C10/10 , Y02P20/152
摘要： A process for separating a first gas from a second gas, having the steps of: (1) contacting one side of a semi-permeable gas separation membrane with a feed gas mixture containing at least the first gas and the second gas, wherein the membrane divides a separation chamber into a high-pressure side into which the feed gas mixture is fed and a low-pressure side; (2) maintaining a pressure differential across the membrane under conditions such that the first gas in the feed gas mixture selectively permeates through the membrane from the high-pressure side to the low-pressure side of the membrane; (3) removing from the low-pressure side of the membrane permeated gas mixture which is enriched in the first gas and depleted in the second gas; and (4) removing from the high-pressure side of the membrane a gas mixture which is enriched in the second gas and depleted in the first gas. The membrane used is made, at least in part, of a thin discriminating layer of polyimide having the repeating formula ##STR1## wherein R is: ##STR2##
摘要翻译：一种用于从第二气体分离第一气体的方法，具有以下步骤：（1）使半透气分离膜的一侧与至少包含第一气体和第二气体的进料气体混合物接触，其中所述膜将分离室分成进料气体混合物供给的高压侧和低压侧; （2）在使得进料气体混合物中的第一气体从膜的高压侧到低压侧选择性渗透通过膜的条件下，保持跨膜的压差; （3）从富含第一气体并且耗尽第二气体的膜渗透气体混合物的低压侧除去; 和（4）从膜的高压侧除去富含第二气体并耗尽第一气体的气体混合物。所使用的膜至少部分地由具有重复式“IMAGE”的聚酰亚胺的薄识别层制成，其中R是：式3或式（I）图式 5 OR 公式6

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