专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5405942A Prepro insulin-like growth factors I and II 失效
标题翻译： Prepro胰岛素样生长因子I和II
公开(公告)号：US5405942A
公开(公告)日：1995-04-11
申请号：US65673
申请日：1987-06-16
申请人： Graeme I. Bell , Leslie B. Rall , James P. Merryweather
发明人： Graeme I. Bell , Leslie B. Rall , James P. Merryweather
IPC分类号： C12N15/00 , A61K38/00 , C07H21/04 , C07K14/65 , C12N15/09 , C12N15/17 , C12P21/00 , C12Q1/68 , C12N15/12
CPC分类号： C07K14/65
摘要： Polynucleotide sequences which encode for human prepro insulin-like growth factors are provided. Such sequences are obtained from the human genome, typically by screening a cDNA library obtained from human liver cells. The polynucleotide sequences may be used for cloning and expression of insulin-like growth factors in suitable hosts, as well as for the production of DNA and RNA which may be used as hybridization probes.E. coli strains HB101(phigf1) and HB101(phigf2) were deposited at the ATCC on Jun. 8, 1984, and granted accession nos. 39729 and 39730, respectively.
摘要翻译：提供编码人前胰岛素样生长因子的多核苷酸序列。通常通过筛选从人肝细胞获得的cDNA文库，从人类基因组获得这样的序列。多核苷酸序列可用于在合适的宿主中克隆和表达胰岛素样生长因子，以及用于产生可用作杂交探针的DNA和RNA。大肠杆菌菌株HB101（phigf1）和HB101（phigf2）于1984年6月8日保藏在ATCC，并授予登录号。 39729和39730。

2. 发明授权

US6017731A Method for expression of heterologous proteins in yeast 失效
公开(公告)号：US6017731A
公开(公告)日：2000-01-25
申请号：US989251
申请日：1997-12-12
申请人： Patricia Tekamp-Olson , James P. Merryweather
发明人： Patricia Tekamp-Olson , James P. Merryweather
IPC分类号： A61K38/22 , A61K38/00 , A61K38/18 , A61P17/02 , C07K1/18 , C07K14/39 , C07K14/49 , C07K14/65 , C07K19/00 , C12N1/19 , C12N15/09 , C12N15/81 , C12P21/02 , C12N15/62
CPC分类号： A61K38/1858 , C07K14/49 , C07K14/65 , C12N15/81 , C07K2319/02
摘要： Compositions and methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form using a yeast host cell as the expression system are provided. Compositions of the invention are nucleotide sequences encoding a signal peptide sequence for a yeast secreted protein, an optional leader peptide sequence for a yeast secreted protein, a native propeptide leader sequence for a mature protein of interest, and a sequence for the mature protein of interest, all operably linked to a yeast promoter. Each of these elements is associated with a processing site recognized in vivo by a yeast proteolytic enzyme. Any or all of these processing sites may be a preferred processing site that has been modified or synthetically derived for more efficient cleavage in vivo. The compositions are useful in methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form. Particularly, vectors comprising these nucleotide coding sequences can be used to transform a yeast host cell, which can then be cultured and screened for secretion of the biologically active mature protein of interest.

3. 发明授权

US4751180A Expression using fused genes providing for protein product 失效
标题翻译：使用提供蛋白质产物的融合基因进行表达
公开(公告)号：US4751180A
公开(公告)日：1988-06-14
申请号：US845737
申请日：1986-03-28
申请人： Lawrence S. Cousens , Patricia A. Tekamp-Olson , Jeffrey R. Shuster , James P. Merryweather
发明人： Lawrence S. Cousens , Patricia A. Tekamp-Olson , Jeffrey R. Shuster , James P. Merryweather
IPC分类号： C07K14/16 , C07K14/62 , C07K14/65 , C12N9/02 , C12N15/62 , C12N15/81 , C12P21/00 , C12N5/00 , C12N15/00 , C12P21/02
CPC分类号： C07K14/005 , C07K14/62 , C07K14/65 , C12N15/62 , C12N15/81 , C12N9/0089 , C12Y115/01001 , C07K2319/00 , C07K2319/02 , C07K2319/50 , C07K2319/75 , C12N2740/16222 , Y10S930/31
摘要： Novel methods and compositions are provided for enhanced yield of heterologous proteins in fungi. The method and compositions involve employing fusion sequences involving a sequence encoding a heterologous product produced in relatively large amount as a stable polypeptide in the host fused to a second sequence in open reading frame with the prior sequence coding for a different heterologous polypeptide, where the two polypeptides are joined by a selectively cleavable linkage. In particular, a sequence coding for superoxide dismutase is joined to another polypeptide of interest at either terminus of the superoxide dismutase in a yeast expression vector under transcriptional control of an active promoter and the vector introduced into a yeast host and the host grown. High yields of the fusion product are obtained in this manner, where the fusion product can be selectively cleaved so as to produce both the superoxide dismutase and the other polypeptide in high yield.The S. cerevisiae strain 2150-2-3 (pYASI1) was deposited at the A.T.C.C. on Feb. 27, 1985 and given accession No. 20745.The S. cerevisiae strain AB110 (pYLUIGF2-14) was deposited at the A.T.C.C. on Mar. 19, 1986 and given accession No. 20796.
摘要翻译：提供了新的方法和组合物用于提高真菌中异源蛋白质的产量。所述方法和组合物涉及使用融合序列，所述融合序列涉及编码在相对大量产生的异源产物的序列，作为在开放阅读框中与第二序列融合的宿主中的稳定多肽，其中先前的序列编码不同的异源多肽，其中两个多肽通过可选择性切割的连接键连接。特别地，编码超氧化物歧化酶的序列在酵母表达载体的超氧化物歧化酶的任一末端与活性启动子的转录控制和导入酵母宿主和宿主生长的载体连接至另一种目的多肽。以这种方式获得高产量的融合产物，其中可以选择性地切割融合产物以便以高产率产生超氧化物歧化酶和另一种多肽。酿酒酵母菌株2150-2-3（pYASI1）保藏在A.T.C.C. 1985年2月27日，加入第20745号。酿酒酵母菌株AB110（pYLUIGF2-14）保藏在A.T.C.C. 1986年3月19日，加拿大号20796。

4. 发明授权

US5342921A Superoxide dismutase fusion polypeptides for expression of mammalian proteins 失效
标题翻译：用于表达哺乳动物蛋白质的超氧化物歧化酶融合多肽
公开(公告)号：US5342921A
公开(公告)日：1994-08-30
申请号：US680046
申请日：1991-03-29
申请人： Lawrence S. Cousens , Patricia A. Tekamp-Olson , Jeffrey R. Shuster , James P. Merryweather
发明人： Lawrence S. Cousens , Patricia A. Tekamp-Olson , Jeffrey R. Shuster , James P. Merryweather
IPC分类号： C07K14/16 , C07K14/62 , C07K14/65 , C12N9/02 , C12N15/81 , C07K3/00 , C07K13/00
CPC分类号： C07K14/005 , C07K14/62 , C07K14/65 , C12N15/81 , C12N9/0089 , C12Y115/01001 , C07K2319/00 , C07K2319/02 , C07K2319/50 , C07K2319/75 , C12N2740/16222 , Y10S435/814
摘要： Novel methods and compositions are provided for enhanced yield of heterologous proteins in fungi. The method and compositions involve employing fusion sequences involving a sequence encoding a heterologous product produced in relatively large amount as a stable polypeptide in the host fused to a second sequence in open reading frame with the prior sequence coding for a different heterologous polypeptide, where the two polypeptides are joined by a selectively cleavable linkage. In particular, a sequence coding for superoxide dismutase is joined to another polypeptide of interest at either terminus of the superoxide dismutase in a yeast expression vector under transcriptional control of an active promoter and the vector introduced into a yeast host and the host grown. High yields of the fusion product are obtained in this manner, where the fusion product can be selectively cleaved so as to produce both the superoxide dismutase and the other polypeptide in high yield.The S. cerevisiae strain 2150-2-3 (pYASI1) was deposited at the A.T.C.C. on Feb. 27, 1985 and given accession no. 20745.The S. cerevisiae strain AB110 (pYLUIGF2-14) was deposited at the A.T.C.C. on Mar. 19, 1986 and given accession no. 20796.
摘要翻译：提供了新的方法和组合物用于提高真菌中异源蛋白质的产量。所述方法和组合物涉及使用融合序列，所述融合序列涉及编码在相对大量产生的异源产物的序列，作为在开放阅读框中与第二序列融合的宿主中的稳定多肽，其中先前的序列编码不同的异源多肽，其中两个多肽通过可选择性切割的连接键连接。特别地，编码超氧化物歧化酶的序列在酵母表达载体的超氧化物歧化酶的任一末端与活性启动子的转录控制和导入酵母宿主和宿主生长的载体连接至另一种目的多肽。以这种方式获得高产量的融合产物，其中可以选择性地切割融合产物以便以高产率产生超氧化物歧化酶和另一种多肽。酿酒酵母菌株2150-2-3（pYASI1）保藏在A.T.C.C. 1985年2月27日，酿酒酵母菌株AB110（pYLUIGF2-14）保藏在A.T.C.C. 1986年3月19日，加拿大号。 20796。

5. 发明授权

US5096825A Gene for human epidermal growth factor and synthesis and expression thereof 失效
标题翻译：人表皮生长因子的基因及其合成及表达
公开(公告)号：US5096825A
公开(公告)日：1992-03-17
申请号：US4212
申请日：1987-01-05
申请人： Philip J. Barr , James P. Merryweather , Guy T. Mullenbach , Mickey S. Urdea , Pablo Valenzuela
发明人： Philip J. Barr , James P. Merryweather , Guy T. Mullenbach , Mickey S. Urdea , Pablo Valenzuela
IPC分类号： C07K14/485 , C12N15/18
CPC分类号： C07K14/485
摘要： A DNA having a base sequence coding for human epidermal growth factor has been synthesized in blocks, and cloned. Novel recombinant DNA transfer vectors containing said cloned DNA have been constructed. The codon usage of the sequence reflects the codon bias of yeast. The DNA sequence is useful for the large scale synthesis of human epidermal growth factor in yeast cells or microorganisms transformed by said recombinant DNA transfer vectors.
摘要翻译：已经合成了具有编码人表皮生长因子的碱基序列的DNA，并克隆。已经构建了含有所述克隆DNA的新型重组DNA转移载体。序列的密码子使用反映了酵母的密码子偏倚。该DNA序列可用于大规模合成酵母细胞中的人表皮生长因子或由所述重组DNA转移载体转化的微生物。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式