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    • 5. 发明授权
    • Automated staining apparatus
    • 自动染色装置
    • US5439649A
    • 1995-08-08
    • US129243
    • 1993-09-29
    • Ken TseungWai Bun WongGlenn K. TakayamaChristopher M. JonesKrishan L. Kalra
    • Ken TseungWai Bun WongGlenn K. TakayamaChristopher M. JonesKrishan L. Kalra
    • G01N1/30B01L3/02G01N1/28G01N1/31G01N35/00G01N35/10G02B21/34B01L11/00
    • B01L3/0275G01N1/312G02B21/34G01N2001/282G01N2001/317G01N2035/103G01N35/0099G01N35/109
    • An automated microscope-slide-staining apparatus, having a supporting framework; an arm moveable in three dimensions attached to the framework; apparatus for moving the arm; a hollow tip head located on the arm; apparatus for alternatively supplying positive or negative gas pressure to the hollow tip head; a removable wash/blow tip having an exit slit, the wash/blow tip being adapted to be removably attached to the hollow tip head by a preselected movement of the arm; a wash/blow tip holder at a first fixed location on the framework; a reagent application tip holder at a second fixed location on the framework for holding a reagent application tip, the reagent application tip being adapted to be removably attached to the hollow tip head by a preselected movement of the arm; a reagent container holder at a third fixed location on the framework; a microscope slide holder at a fourth fixed location on the framework, the microscope slide holder being adapted to removably contain the microscope slide; and control apparatus for controlling movement of the arm between the locations, whereby the tip head picks up the wash/blow tip or the reagent application tip in response to movement of the arm under control of the control apparatus and moves to one or more of the locations to pick up a reagent in the reagent container or dispense the reagent on the slide or to dispense a gas through the wash/blow tip over the slide. Components of the apparatus and methods of staining are also part of the invention.
    • 具有支撑框架的自动显微镜载玻片染色装置; 一个可移动的臂可以三维地连接到框架上; 用于移动臂的装置; 位于手臂上的中空尖头; 用于交替地向中空尖端头供应正或负气体压力的装置; 具有出口狭缝的可移除的洗涤/吹塑尖端,所述洗涤/吹塑尖端适于通过所述臂的预选运动可移除地附接到所述中空末端头部; 在框架上的第一固定位置处的洗涤/吹头保持器; 用于保持试剂应用尖端的框架上的第二固定位置处的试剂施用末端保持器,所述试剂施加末端适于通过所述臂的预选运动可移除地附接到所述中空末端头; 在框架上的第三固定位置的试剂容器支架; 在框架上的第四固定位置处的显微镜载玻片保持器,显微镜载玻片保持器适于可拆卸地容纳显微镜载玻片; 以及用于控制所述臂在所述位置之间的移动的控制装置,由此所述尖端头在所述控制装置的控制下响应于所述臂的移动而拾取所述洗涤/吹出尖端或所述试剂施加末端,并移动到所述控制装置中的一个或多个 在试剂容器中取出试剂的位置或者将试剂分配在载玻片上,或者通过载玻片上的洗涤/吹塑尖端分配气体。 设备的组分和染色方法也是本发明的一部分。