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    • 1. 发明授权
    • Simultaneous amplification of multiple targets
    • 同时放大多个目标
    • US5624825A
    • 1997-04-29
    • US451313
    • 1995-05-26
    • George T. WalkerJames G. NadeauMichael C. Little
    • George T. WalkerJames G. NadeauMichael C. Little
    • G01N33/566C07H21/04C12N15/09C12P19/34C12Q1/68G11B5/00G11B5/09G11B5/187G11B5/31G11B5/455G11B5/465G11B19/04
    • C12Q1/6853
    • Methods for multiplex amplification of target nucleic acid sequences using a single pair of primers. Defined sequences are appended to the ends of multiple target sequences as part of the amplification reaction so that no steps in addition to amplification are required. The target sequences with the appended defined sequences need not be isolated prior to amplification. In one embodiment for coamplification of two target sequences, a sequence corresponding to a terminal segment of the first target sequence is appended to one end of the second target sequence and a sequence corresponding to a terminal segment of the second target sequence is appended to one end of the first target sequence. Amplification of the two targets then requires only a single pair of primers. Alternatively, a single defined sequence may be appended to the 5' and 3' ends of any number of selected targets. All such modified target sequences may then be amplified using a single pair of primers which hybridize to the defined end-sequences.
    • 使用单对引物多重扩增靶核酸序列的方法。 定义的序列作为扩增反应的一部分附加到多个靶序列的末端,因此不需要扩增步骤。 具有附加定义序列的靶序列不需要在扩增前分离。 在两个目标序列的共放大的一个实施例中,对应于第一目标序列的末端片段的序列被附加到第二目标序列的一端,并且与第二目标序列的末端片段相对应的序列被附加到一端 的第一个靶序列。 两个靶标的扩增只需要一对引物即可。 或者,单个定义的序列可以附加到任意数目的所选目标的5'和3'端。 然后可以使用与定义的末端序列杂交的单对引物扩增所有这些修饰的靶序列。
    • 3. 发明授权
    • Simultaneous amplification of multiple targets
    • 同时放大多个目标
    • US5422252A
    • 1995-06-06
    • US73197
    • 1993-06-04
    • George T. WalkerJames G. NadeauMichael C. Little
    • George T. WalkerJames G. NadeauMichael C. Little
    • G01N33/566C07H21/04C12N15/09C12P19/34C12Q1/68G11B5/00G11B5/09G11B5/187G11B5/31G11B5/455G11B5/465G11B19/04C12Q1/70
    • C12Q1/6853
    • Methods for multiplex amplification of target nucleic acid sequences using a single pair of primers. Defined sequences are appended to the ends of multiple target sequences as part of the amplification reaction so that no steps in addition to amplification are required. The target sequences with the appended defined sequences need not be isolated prior to amplification. In one embodiment for coamplification of two target sequences, a sequence corresponding to a terminal segment of the first target sequence is appended to one end of the second target sequence and a sequence corresponding to a terminal segment of the second target sequence is appended to one end of the first target sequence. Amplification of the two targets then requires only a single pair of primers. Alternatively, a single defined sequence may be appended to the 5' and 3' ends of any number of selected targets. All such modified target sequences may then be amplified using a single pair of primers which hybridize to the defined end-sequences.
    • 使用单对引物多重扩增靶核酸序列的方法。 定义的序列作为扩增反应的一部分附加到多个靶序列的末端,因此不需要扩增步骤。 具有附加定义序列的靶序列不需要在扩增前分离。 在两个目标序列的共放大的一个实施例中,对应于第一目标序列的末端片段的序列被附加到第二目标序列的一端,并且与第二目标序列的末端片段相对应的序列被附加到一端 的第一个靶序列。 两个靶标的扩增只需要一对引物即可。 或者,单个定义的序列可以附加到任意数目的所选目标的5'和3'端。 然后可以使用与定义的末端序列杂交的单对引物扩增所有这些修饰的靶序列。
    • 4. 再颁专利
    • Detection of nucleic acid amplification
    • 检测核酸扩增
    • USRE39885E1
    • 2007-10-16
    • US09082247
    • 1998-05-20
    • James G. NadeauGeorge T. Walker
    • James G. NadeauGeorge T. Walker
    • C12Q1/68C12P19/34
    • C12Q1/6844C12Q2565/525C12Q2561/113C12Q2531/119
    • Methods for detecting, immobilizing or localizing primer extension products of a Strand Displacement Amplification reaction which are coupled to, and an indication of, amplification of the target sequence. The primer extension products are secondary, target-specific DNA products generated concurrently with SDA of the target sequence and can therefore be used to detect and/or measure target sequence amplification in real-time. In general, the secondary amplification products are not amplifiable and remain inert in the SDA reaction after they are formed without interfering with amplification of the target sequence. The secondary amplification products may be designed or modified to contain special features to facilitate their detection, immobilization or localization.
    • 用于检测,固定或定位连接于链序列扩增反应的引物延伸产物的方法,以及扩增靶序列的指示。 引物延伸产物是与靶序列的SDA同时产生的次要靶特异性DNA产物,因此可用于实时检测和/或测量靶序列扩增。 通常,二次扩增产物在其形成后不能扩增并在SDA反应中保持惰性,而不干扰靶序列的扩增。 二次扩增产物可以被设计或修改以包含特殊特征以促进其检测,固定或定位。
    • 7. 发明授权
    • Detection of nucleic acid amplification
    • 检测核酸扩增
    • US5547861A
    • 1996-08-20
    • US229281
    • 1994-04-18
    • James G. NadeauGeorge T. Walker
    • James G. NadeauGeorge T. Walker
    • C12N15/09C12Q1/68C12R1/32C12P19/34C07H21/04C12Q1/70
    • C12Q1/6844
    • Methods for detecting, immobilizing or localizing primer extension products of a Strand Displacement Amplification reaction which are coupled to, and an indication of, amplification of the target sequence. The primer extension products are secondary, target-specific DNA products generated concurrently with SDA of the target sequence and can therefore be used to detect and/or measure target sequence amplification in real-time. In general, the secondary amplification products are not amplifiable and remain inert in the SDA reaction after they are formed without interfering with amplification of the target sequence. The secondary amplification products may be designed or modified to contain special features to facilitate their detection, immobilization or localization.
    • 用于检测,固定或定位连接于链序列扩增反应的引物延伸产物的方法,以及扩增靶序列的指示。 引物延伸产物是与靶序列的SDA同时产生的次要靶特异性DNA产物,因此可用于实时检测和/或测量靶序列扩增。 通常,二次扩增产物在其形成后不能扩增并在SDA反应中保持惰性,而不干扰靶序列的扩增。 二次扩增产物可以被设计或修改以包含特殊特征以促进其检测,固定或定位。