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1. 发明授权

US07531316B2 High throughput assay of Lp-PLA2 activity 有权
标题翻译： Lp-PLA2活性的高通量测定
公开(公告)号：US07531316B2
公开(公告)日：2009-05-12
申请号：US10557540
申请日：2004-05-27
申请人： Yun-Fu Hu , George T. Walker
发明人： Yun-Fu Hu , George T. Walker
IPC分类号： C12Q1/34
CPC分类号： G01N33/573 , C12N9/20 , C12Q1/44 , G01N2333/918
摘要： This invention relates to a method for determining the activity of Lp-PLA2 in a plurality of samples from animals. The invention also relates to a kit for determining Lp-PLA2 activity in a plurality of samples.
摘要翻译：本发明涉及一种用于确定来自动物的多个样品中Lp-PLA2的活性的方法。本发明还涉及用于确定多个样品中的Lp-PLA2活性的试剂盒。

2. 发明授权

US07741020B2 Methods for detecting Lp-PLA2 activity and inhibition of Lp-PLA2 activity 失效
标题翻译：检测Lp-PLA2活性和抑制Lp-PLA2活性的方法
公开(公告)号：US07741020B2
公开(公告)日：2010-06-22
申请号：US11106239
申请日：2005-04-14
申请人： Yaping Shou , Yin-Fai Siu , George T. Walker
发明人： Yaping Shou , Yin-Fai Siu , George T. Walker
IPC分类号： C12Q1/00
CPC分类号： C12Q1/61 , C12Q1/34 , C12Q1/44 , C12Q2334/10 , G01N2333/918
摘要： This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.
摘要翻译：本发明涉及用于测定来自动物的至少一种样品中Lp-PLA2的活性的方法。本发明还涉及用于测定来自施用Lp-PLA2抑制剂的动物的样品中Lp-PLA2活性的抑制的方法。

3. 再颁专利

USRE39885E1 Detection of nucleic acid amplification 失效
标题翻译：检测核酸扩增
公开(公告)号：USRE39885E1
公开(公告)日：2007-10-16
申请号：US09082247
申请日：1998-05-20
申请人： James G. Nadeau , George T. Walker
发明人： James G. Nadeau , George T. Walker
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6844 , C12Q2565/525 , C12Q2561/113 , C12Q2531/119
摘要： Methods for detecting, immobilizing or localizing primer extension products of a Strand Displacement Amplification reaction which are coupled to, and an indication of, amplification of the target sequence. The primer extension products are secondary, target-specific DNA products generated concurrently with SDA of the target sequence and can therefore be used to detect and/or measure target sequence amplification in real-time. In general, the secondary amplification products are not amplifiable and remain inert in the SDA reaction after they are formed without interfering with amplification of the target sequence. The secondary amplification products may be designed or modified to contain special features to facilitate their detection, immobilization or localization.
摘要翻译：用于检测，固定或定位连接于链序列扩增反应的引物延伸产物的方法，以及扩增靶序列的指示。引物延伸产物是与靶序列的SDA同时产生的次要靶特异性DNA产物，因此可用于实时检测和/或测量靶序列扩增。通常，二次扩增产物在其形成后不能扩增并在SDA反应中保持惰性，而不干扰靶序列的扩增。二次扩增产物可以被设计或修改以包含特殊特征以促进其检测，固定或定位。

4. 发明授权

US5561044A Detection of mycobacteria by multiplex strand displacement nucleic acid amplification 失效
标题翻译：通过多重链置换核酸扩增检测分枝杆菌
公开(公告)号：US5561044A
公开(公告)日：1996-10-01
申请号：US398305
申请日：1995-03-03
申请人： George T. Walker , James G. Nadeau , Patricia A. Spears , Colleen M. Nycz , Daryl D. Shank , James L. Schram , Stewart R. Jurgensen
发明人： George T. Walker , James G. Nadeau , Patricia A. Spears , Colleen M. Nycz , Daryl D. Shank , James L. Schram , Stewart R. Jurgensen
IPC分类号： C12N15/09 , C12Q1/04 , C12Q1/68 , C12R1/32 , C12R1/33 , C12P19/34
CPC分类号： C12Q1/689 , C12Q1/6851 , C12Q2600/16
摘要： Primers and methods for adapter-mediated multiplex amplification of the IS6110 insertion element of Mycobacterium tuberculosis (M.tb) and the 16S ribosomal gene of Mycobacterium tuberculosis, useful for simultaneously detecting and/or identifying species of the M. tuberculosis complex and other clinically relevant Mycobacterium species. Multiplex Strand Displacement Amplification (SDA) is used in a single amplification reaction which is capable of simultaneously identifying M. tuberculosis and providing a screen for substantially all of the clinically relevant species of Mycobacteria. Also disclosed are methods for adapter-mediated multiplex amplification of multiple target sequences and a single internal control sequence for determination of sample efficacy or quantitation of the targets. In a preferred embodiment, an internal control sequence is included in the amplification reaction and coamplified with the IS6110 and 16S target sequences as an indication of sample amplification activity or to quantitate the initial amount of target sequences in the sample.
摘要翻译：针对结核分枝杆菌（M.tb）的IS6110插入元件和结核分枝杆菌的16S核糖体基因的适配器介导的多重扩增的引物和方法，可用于同时检测和/或鉴定结核分枝杆菌复合物和其他临床相关的物种分枝杆菌种。多重链位移扩增（SDA）用于能够同时鉴定结核分枝杆菌并为基本上所有临床相关分枝杆菌物种提供筛选的单一扩增反应。还公开了用于多重靶序列的衔接子介导的多重扩增的方法和用于确定靶的样品功效或定量的单个内部控制序列的方法。在优选的实施方案中，内部对照序列包括在扩增反应中并与IS6110和16S靶序列共扩增，作为样品扩增活性的指示或定量样品中靶序列的初始量。

5. 发明授权

US5470723A Detection of mycobacteria by multiplex nucleic acid amplification 失效
标题翻译：通过多重核酸扩增检测分枝杆菌
公开(公告)号：US5470723A
公开(公告)日：1995-11-28
申请号：US111076
申请日：1993-08-24
申请人： George T. Walker , James G. Nadeau , Patricia A. Spears , Colleen M. Nycz , Daryl D. Shank , James L. Schram , Stewart R. Jurgensen
发明人： George T. Walker , James G. Nadeau , Patricia A. Spears , Colleen M. Nycz , Daryl D. Shank , James L. Schram , Stewart R. Jurgensen
IPC分类号： C12N15/09 , C12Q1/04 , C12Q1/68 , C12R1/32 , C12R1/33 , C07H15/12 , C07H17/00 , C12P19/34
CPC分类号： C12Q1/689 , C12Q1/6851 , C12Q2600/16
摘要： Primers and methods for adapter-mediated multiplex amplification of the IS6110 insertion element of Mycobacterium tuberculosis (M.tb) and the 16S ribosomal gene of Mycobacterium tuberculosis, useful for simultaneously detecting and/or identifying species of the M. tuberculosis complex and other clinically relevant Mycobacterium species. Multiplex Strand Displacement Amplification (SDA) is used in a single amplification reaction which is capable of simultaneously identifying M. tuberculosis and providing a screen for substantially all of the clinically relevant species of Mycobacteria. Also disclosed are methods for adapter-mediated multiplex amplification of multiple target sequences and a single internal control sequence for determination of sample efficacy or quantitation of the targets. In a preferred embodiment, an internal control sequence is included in the amplification reaction and coamplified with the IS6110 ant 16S target sequences as an indication of sample amplification activity or to quantitate the initial amount of target sequences in the sample.
摘要翻译：针对结核分枝杆菌（M.tb）的IS6110插入元件和结核分枝杆菌的16S核糖体基因的适配器介导的多重扩增的引物和方法，可用于同时检测和/或鉴定结核分枝杆菌复合物和其他临床相关的物种分枝杆菌种。多重链位移扩增（SDA）用于能够同时鉴定结核分枝杆菌并为基本上所有临床相关分枝杆菌物种提供筛选的单一扩增反应。还公开了用于多重靶序列的衔接子介导的多重扩增的方法和用于确定靶的样品功效或定量的单个内部控制序列的方法。在优选的实施方案中，扩增反应中包含内部控制序列并与IS6110蚂蚁16S靶序列共扩增，作为样品扩增活性的指示或定量样品中靶序列的初始量。

6. 发明授权

US08609357B2 Methods for detecting LP-PLA2 activity and inhibition of LP-PLA2 activity 有权
标题翻译：检测LP-PLA2活性和抑制LP-PLA2活性的方法
公开(公告)号：US08609357B2
公开(公告)日：2013-12-17
申请号：US13540571
申请日：2012-07-02
申请人： Yaping Shou , Yin-Fai Siu , George T. Walker
发明人： Yaping Shou , Yin-Fai Siu , George T. Walker
IPC分类号： C12Q1/44 , C12Q1/00
CPC分类号： C12Q1/61 , C12Q1/34 , C12Q1/44 , C12Q2334/10 , G01N2333/918
摘要： This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.
摘要翻译：本发明涉及用于测定来自动物的至少一种样品中Lp-PLA2的活性的方法。本发明还涉及用于测定来自施用Lp-PLA2抑制剂的动物的样品中Lp-PLA2活性的抑制的方法。

7. 发明授权

US08368882B2 Systems and methods for detecting a signal and applying thermal energy to a signal transmission element 有权
标题翻译：用于检测信号并向信号传输元件施加热能的系统和方法
公开(公告)号：US08368882B2
公开(公告)日：2013-02-05
申请号：US12696406
申请日：2010-01-29
申请人： David Opalsky , George T. Walker , Norman C. Nelson , Richard S. Lee , Sara H. Fan
发明人： David Opalsky , George T. Walker , Norman C. Nelson , Richard S. Lee , Sara H. Fan
IPC分类号： G01N21/01
CPC分类号： G01N21/03 , B01L3/5027 , B01L3/505 , B01L7/52 , B01L9/527 , B01L2200/04 , B01L2200/146 , B01L2300/021 , B01L2300/044 , B01L2300/0654 , B01L2300/0672 , B01L2300/0816 , B01L2300/087 , B01L2300/1827 , B01L2400/043 , B01L2400/0478 , B01L2400/0481 , B01L2400/0487 , B01L2400/0605 , B01L2400/0666 , G01N21/0332 , G01N2021/0328 , G01N2021/0346 , G01N2035/00346
摘要： A signal detection system configured for detecting a signal emitted by the contents of a reaction receptacle is also configured to apply thermal energy to a portion of the reaction receptacle to affect a reaction occurring within the reaction receptacle. More particularly, a system for detecting electromagnetic radiation emitted by the contents of a reaction receptacle includes a transmission element configured for transmitting electromagnetic radiation from the contents of the receptacle, a thermal element associated with the transmission element and configured to apply thermal energy to at least a portion of the receptacle, and a detector configured to receive electromagnetic radiation from the transmission element and to generate a signal corresponding to a characteristic of the electromagnetic radiation received by the detector.
摘要翻译：被配置为检测由反应容器的内容物发射的信号的信号检测系统还被配置为将热能施加到反应容器的一部分以影响在反应容器内发生的反应。更具体地，用于检测由反应容器的内容物发射的电磁辐射的系统包括传输元件，其被配置用于从容器的内容物传输电磁辐射，与传输元件相关联的热元件，并被配置为将热能施加到至少所述插座的一部分以及被配置为从所述传输元件接收电磁辐射并且产生对应于由所述检测器接收的电磁辐射的特性的信号的检测器。

8. 发明授权

US5547861A Detection of nucleic acid amplification 失效
标题翻译：检测核酸扩增
公开(公告)号：US5547861A
公开(公告)日：1996-08-20
申请号：US229281
申请日：1994-04-18
申请人： James G. Nadeau , George T. Walker
发明人： James G. Nadeau , George T. Walker
IPC分类号： C12N15/09 , C12Q1/68 , C12R1/32 , C12P19/34 , C07H21/04 , C12Q1/70
CPC分类号： C12Q1/6844
摘要： Methods for detecting, immobilizing or localizing primer extension products of a Strand Displacement Amplification reaction which are coupled to, and an indication of, amplification of the target sequence. The primer extension products are secondary, target-specific DNA products generated concurrently with SDA of the target sequence and can therefore be used to detect and/or measure target sequence amplification in real-time. In general, the secondary amplification products are not amplifiable and remain inert in the SDA reaction after they are formed without interfering with amplification of the target sequence. The secondary amplification products may be designed or modified to contain special features to facilitate their detection, immobilization or localization.
摘要翻译：用于检测，固定或定位连接于链序列扩增反应的引物延伸产物的方法，以及扩增靶序列的指示。引物延伸产物是与靶序列的SDA同时产生的次要靶特异性DNA产物，因此可用于实时检测和/或测量靶序列扩增。通常，二次扩增产物在其形成后不能扩增并在SDA反应中保持惰性，而不干扰靶序列的扩增。二次扩增产物可以被设计或修改以包含特殊特征以促进其检测，固定或定位。

9. 发明授权

US5536649A Decontamination of nucleic acid amplification reactions using uracil-N-glycosylase (UDG) 失效
标题翻译：使用尿嘧啶-N-糖基化酶（UDG）进行核酸扩增反应的去污
公开(公告)号：US5536649A
公开(公告)日：1996-07-16
申请号：US283117
申请日：1994-07-29
申请人： Melinda S. Fraiser , George T. Walker , James L. Schram
发明人： Melinda S. Fraiser , George T. Walker , James L. Schram
IPC分类号： C12N15/09 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6844 , C12Q1/6848
摘要： Methods for inactivating contaminating amplicons in isothermal nucleic acid amplification reactions such as SDA. Uracil is incorporated into the amplicons produced by amplification in the place of thymine (T) using novel SDA reaction conditions. If these amplicons contaminate a subsequent amplification reaction, they may be inactivated as templates (i.e., rendered unamplifiable) by treatment with UDG. As isothermal amplification does not involve elevated temperatures, the UDG may be inactivated during the subsequent amplification of specific target sequences by inclusion of the UDG inhibitor protein Ugi. Incorporation of dU has unexpectedly been found to enhance the amplification power of SDA as compared to conventional SDA reactions. The methods may also be used to detect UDG activity in reagents or samples.
摘要翻译：在等温核酸扩增反应（如SDA）中灭活污染扩增子的方法。尿嘧啶通过使用新的SDA反应条件通过扩增代替胸腺嘧啶（T）而产生的扩增子。如果这些扩增子污染随后的扩增反应，则它们可以通过用UDG处理作为模板灭活（即，使其不能扩增）。由于等温扩增不包括升高的温度，所以UDG可以在随后通过包含UDG抑制剂蛋白Ugi扩增特异性靶序列期间失活。与传统的SDA反应相比，意外地发现dU的引入增强了SDA的放大功率。该方法也可用于检测试剂或样品中的UDG活性。

10. 发明授权

US08846309B2 Methods for detecting Lp-PLA2 activity and inhibition of Lp-PLA2 activity 有权
标题翻译：检测Lp-PLA2活性和抑制Lp-PLA2活性的方法
公开(公告)号：US08846309B2
公开(公告)日：2014-09-30
申请号：US12817677
申请日：2010-06-17
申请人： Yaping Shou , Yin-Fai Siu , George T. Walker
发明人： Yaping Shou , Yin-Fai Siu , George T. Walker
IPC分类号： C12Q1/00 , C12Q1/61 , C12Q1/34 , C12Q1/44
CPC分类号： C12Q1/61 , C12Q1/34 , C12Q1/44 , C12Q2334/10 , G01N2333/918
摘要： This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.
摘要翻译：本发明涉及用于测定来自动物的至少一种样品中Lp-PLA2的活性的方法。本发明还涉及用于测定来自施用Lp-PLA2抑制剂的动物的样品中Lp-PLA2活性的抑制的方法。

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