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1. 发明授权

US07816103B2 Protein and process for preparing the same 失效
标题翻译：蛋白质及其制备方法
公开(公告)号：US07816103B2
公开(公告)日：2010-10-19
申请号：US11833653
申请日：2007-08-03
申请人： Fusao Makishima , Hiroyuki Takamatsu , Hideo Miki , Shinji Kawai , Michio Kimura , Tomoaki Matsumoto , Mieko Katsuura , Koichi Enomoto , Yusuke Satoh
发明人： Fusao Makishima , Hiroyuki Takamatsu , Hideo Miki , Shinji Kawai , Michio Kimura , Tomoaki Matsumoto , Mieko Katsuura , Koichi Enomoto , Yusuke Satoh
IPC分类号： C12P21/02 , C12N1/21 , C12N15/70 , C07K14/51
CPC分类号： C07K14/51 , A61K38/00 , Y10S514/801
摘要： A protein having amino acid sequence in SEQ ID No.:1 of the Sequence Listing derived from human MP52, and a dimer protein thereof. A homodimer protein described above can be obtained by constructing a plasmid containing DNA coding amino acid sequence in SEQ ID No.:1 of the Sequence Listing with a methionine at the N-terminus, introducing the plasmid into E. coli for transformation, solubilizing inclusion bodies obtained by culturing the transformant, purifying the monomer protein from the solubilized solution, refolding the monomer protein into a dimer protein and purifying the same. The homodimer protein described above is useful as a pharmaceutical composition for treating cartilage and bone diseases.
摘要翻译：衍生自人MP52的序列表的SEQ ID No.1中具有氨基酸序列的蛋白质及其二聚体蛋白质。上述同型二聚体蛋白可以通过在N末端构建含有序列表SEQ ID No.1的DNA编码氨基酸序列的质粒与甲硫氨酸，将质粒导入大肠杆菌进行转化，溶解包合物通过培养转化体获得的物质，从溶解的溶液中纯化单体蛋白质，将单体蛋白质重折叠成二聚体蛋白质并进行纯化。上述同型二聚体蛋白质可用作治疗软骨和骨疾病的药物组合物。

2. 发明授权

US07235527B2 Protein and process for producing the same 失效
标题翻译：蛋白质及其生产方法
公开(公告)号：US07235527B2
公开(公告)日：2007-06-26
申请号：US08945459
申请日：1996-04-19
申请人： Fusao Makishima , Hiroyuki Takamatsu , Hideo Miki , Shinji Kawai , Michio Kimura , Tomoaki Matsumoto , Mieko Katsuura , Koichi Enomoto , Yusuke Satoh
发明人： Fusao Makishima , Hiroyuki Takamatsu , Hideo Miki , Shinji Kawai , Michio Kimura , Tomoaki Matsumoto , Mieko Katsuura , Koichi Enomoto , Yusuke Satoh
IPC分类号： A61K38/18 , A61K38/39 , A61K9/14 , C07K14/51
CPC分类号： C07K14/51 , A61K38/00 , Y10S514/801
摘要： A protein consisting of the amino acid sequence in SEQ ID No: 1 derived from human MP52 and a homodimer protein thereof useful for treating cartilage and/or bone diseases and a process for its preparation.
摘要翻译：由源自人MP52的SEQ ID No：1的氨基酸序列和用于治疗软骨和/或骨疾病的同二聚体蛋白质组成的蛋白质及其制备方法。

3. 发明授权

US07268114B2 Protein and process for preparing the same 失效
标题翻译：蛋白质及其制备方法
公开(公告)号：US07268114B2
公开(公告)日：2007-09-11
申请号：US10365231
申请日：2003-02-12
申请人： Fusao Makishima , Hiroyuki Takamatsu , Hideo Miki , Shinji Kawai , Michio Kimura , Tomoaki Matsumoto , Mieko Katsuura , Koichi Enomoto , Yusuke Satoh
发明人： Fusao Makishima , Hiroyuki Takamatsu , Hideo Miki , Shinji Kawai , Michio Kimura , Tomoaki Matsumoto , Mieko Katsuura , Koichi Enomoto , Yusuke Satoh
IPC分类号： A61K38/18 , C07K14/51 , C12N15/12 , C12N1/21 , C12P21/02 , A61F2/30 , A61F2/28
CPC分类号： C07K14/51 , A61K38/00 , Y10S514/801
摘要： A protein having amino acid sequence in SEQ ID NO: 1 of the Sequence Listing derived from human MP52, and a dimer protein thereof. A homodimer protein described above can be obtained by constructing a plasmid containing DNA coding amino acid sequence in SEQ ID NO: 1 of the Sequence Listing with a methionine at the N-terminus, introducing the plasmid into E. coli for transformation, solubilizing inclusion bodies obtained by culturing the transformant, purifying the monomer protein from the solubilized solution, refolding the monomer protein into a dimer protein and purifying the same. The homodimer protein described above is useful as a pharmaceutical composition for treating cartilage and bone diseases.
摘要翻译：衍生自人MP52的序列表的SEQ ID NO：1中具有氨基酸序列的蛋白质及其二聚体蛋白质。可以通过在N末端构建含有序列表的SEQ ID NO：1的DNA编码氨基酸序列的质粒与甲硫氨酸在N末端引入，将质粒导入大肠杆菌进行转化，从而增溶包涵体通过培养转化体获得，从溶解的溶液中纯化单体蛋白质，将单体蛋白重折叠成二聚体蛋白质并进行纯化。上述同型二聚体蛋白质可用作治疗软骨和骨疾病的药物组合物。

4. 发明授权

US07638129B2 Monomer protein with bone morphogenetic activity and medicinal agent containing the same for preventing and treating diseases of cartilage and bone 失效
标题翻译：具有骨形态发生活性的单体蛋白和含有预防和治疗软骨和骨骼疾病的药物
公开(公告)号：US07638129B2
公开(公告)日：2009-12-29
申请号：US11758878
申请日：2007-06-06
申请人： Shinji Kawai , Michio Kimura , Yoshifumi Muraki , Mieko Katsuura
发明人： Shinji Kawai , Michio Kimura , Yoshifumi Muraki , Mieko Katsuura
IPC分类号： A61K38/18 , C07K14/435
CPC分类号： C07K14/51 , A61K38/00
摘要： This invention provides for a protein of the TGF-β superfamily in which the cysteine involved in the normal formation of homodimers is changed to another amino acid. These mutant proteins, as monomers, display higher bone morphogenetic activity than the wild-type protein dimers. Also provided is a method for producing and isolating these monomers by plasmid driven expression in various host systems including E. coli. In addition, the invention discloses the use of an agent containing purified monomers in preventing and treating diseases and problems affecting bone and/or cartilage.
摘要翻译：本发明提供了TGF-β超家族的蛋白质，其中参与同型二聚体正常形成的半胱氨酸改变为另一种氨基酸。这些突变蛋白，作为单体，显示比野生型蛋白二聚体更高的骨形态发生活性。还提供了通过在包括大肠杆菌的各种宿主系统中的质粒驱动表达来产生和分离这些单体的方法。此外，本发明公开了含有纯化单体的药剂在预防和治疗疾病和影响骨和/或软骨的问题中的用途。

5. 发明授权

US07235241B2 Monomer protein with bone morphogenetic activity and medicinal agent containing the same for preventing and treating diseases of cartilage and bone 有权
标题翻译：具有骨形态发生活性的单体蛋白和含有预防和治疗软骨和骨骼疾病的药物
公开(公告)号：US07235241B2
公开(公告)日：2007-06-26
申请号：US10751451
申请日：2004-01-06
申请人： Shinji Kawai , Michio Kimura , Yoshifumi Muraki , Mieko Katsuura
发明人： Shinji Kawai , Michio Kimura , Yoshifumi Muraki , Mieko Katsuura
IPC分类号： A61K38/18 , C07K14/475 , C12N1/20 , C12P21/06 , C07H21/04
CPC分类号： C07K14/51 , A61K38/00
摘要： This invention provides for a protein of the TGF-β superfamily in which the cysteine involved in the normal formation of homodimers is changed to another amino acid. These mutant proteins, as monomers, display higher bone morphogenetic activity than the wild-type protein dimers. Also provided is a method for producing and isolating these monomers by plasmid driven expression in various host systems including E. coli. In addition, the invention discloses the use of an agent containing purified monomers in preventing and treating diseases and problems affecting bone and/or cartilage.
摘要翻译：本发明提供了TGF-β超家族的蛋白质，其中参与同型二聚体正常形成的半胱氨酸改变为另一种氨基酸。这些突变蛋白，作为单体，显示比野生型蛋白二聚体更高的骨形态发生活性。还提供了通过在包括大肠杆菌的各种宿主系统中的质粒驱动表达来产生和分离这些单体的方法。此外，本发明公开了含有纯化单体的药剂在预防和治疗疾病和影响骨和/或软骨的问题中的用途。

6. 发明申请

US20060019886A1 Monomer protein with bone morphogenetic activity and medicinal agent containing the same for preventing and treating diseases of cartilage and bone 有权
公开(公告)号：US20060019886A1
公开(公告)日：2006-01-26
申请号：US10751451
申请日：2004-01-06
申请人： Shinji Kawai , Michio Kimura , Yoshifumi Muraki , Mieko Katsuura
发明人： Shinji Kawai , Michio Kimura , Yoshifumi Muraki , Mieko Katsuura
IPC分类号： A61K38/18 , C07K14/495
CPC分类号： C07K14/51 , A61K38/00
摘要： This invention provides for a protein of the TGF-β superfamily in which the cysteine involved in the normal formation of homodimers is changed to another amino acid. These mutant proteins, as monomers, display higher bone morphogenetic activity than the wild-type protein dimers. Also provided is a method for producing and isolating these monomers by plasmid driven expression in various host systems including E. coli. In addition, the invention discloses the use of an agent containing purified monomers in preventing and treating diseases and problems affecting bone and/or cartilage.

7. 发明授权

US07132397B1 Bone morphogenetic protein antagonist based on the mature protein 有权
标题翻译：基于成熟蛋白的骨形态发生蛋白拮抗剂
公开(公告)号：US07132397B1
公开(公告)日：2006-11-07
申请号：US09806368
申请日：1999-10-04
申请人： Mieko Katsuura , Michio Kimura
发明人： Mieko Katsuura , Michio Kimura
IPC分类号： A61K38/18 , C07K14/51
CPC分类号： C07K14/51 , A61K38/00
摘要： The purpose is to provide a mature protein having an antagonistic activity against bone morphogenetic proteins. The mature protein having an antagonistic activity against bone morphogenetic proteins is obtained by converting at least one residue among methionine residues or tryptophane residues existing in the amino acid sequence of mature human MP52 (SEQ ID No 1) to a hydrophilic residue by chemical modification, or replacing said residues with a hydrophilic amino acid residue or a polar amino acid residue. The chemical modification for said methionine residue is performed by an oxidization reaction or an alkylation reaction. The chemical modification for said tryptophane residue is performed by an allylsulphenylation reaction. Or a mature protein having an antagonistic activity against bone morphogenetic proteins is obtained by converting at least one residue of tryptophane residues existing in the amino acid sequences of mature human BMP-2 (SEQ ID No 2), mature human BMP-4 (SEQ ID No 3), and mature human BMP-7 (SEQ ID No 4) to a hydrophilic residue by chemical modification, or replacing said residues with a hydrophilic amino acid residue or a polar amino acid residue.
摘要翻译：目的是提供对骨形态发生蛋白具有拮抗作用的成熟蛋白质。通过化学修饰将成熟人MP52（SEQ ID No.1）的氨基酸序列中存在的甲硫氨酸残基或色氨酸残基中的至少一个残基转化成亲水性残基，获得对骨形态发生蛋白具有拮抗作用的成熟蛋白质，或用亲水氨基酸残基或极性氨基酸残基取代所述残基。所述甲硫氨酸残基的化学修饰通过氧化反应或烷基化反应进行。所述色氨酸残基的化学修饰通过烯丙基苯基化反应进行。或者通过转化成熟人BMP-2（SEQ ID No 2），成熟人BMP-4（SEQ ID No.2）的氨基酸序列中存在的色氨酸残基的至少一个残基，获得对骨形态发生蛋白具有拮抗活性的成熟蛋白质 No.3）和成熟的人BMP-7（SEQ ID No 4）通过化学修饰与亲水性残基反应，或用亲水氨基酸残基或极性氨基酸残基替代所述残基。

8. 发明授权

US08216668B2 Resin composition, insulating sheet with base, prepreg, multilayer printed wiring board and semiconductor device 有权
标题翻译：树脂组合物，具有基材的绝缘片，预浸料，多层印刷线路板和半导体器件
公开(公告)号：US08216668B2
公开(公告)日：2012-07-10
申请号：US12443822
申请日：2007-10-02
申请人： Michio Kimura
发明人： Michio Kimura
IPC分类号： B32B3/00
CPC分类号： H05K3/4626 , B32B5/022 , B32B5/024 , B32B15/08 , B32B15/14 , B32B15/20 , B32B27/16 , B32B27/18 , B32B27/20 , B32B27/26 , B32B27/28 , B32B27/38 , B32B2260/021 , B32B2260/046 , B32B2262/02 , B32B2262/0261 , B32B2262/0269 , B32B2262/0276 , B32B2262/0284 , B32B2262/10 , B32B2262/101 , B32B2264/102 , B32B2307/206 , B32B2307/306 , B32B2307/3065 , B32B2307/308 , B32B2307/50 , B32B2307/538 , B32B2307/72 , B32B2307/7265 , B32B2457/00 , B32B2457/08 , C08K5/5425 , C08K5/548 , C08L63/00 , H05K3/389 , H05K2201/0209 , Y10T428/24917 , Y10T428/24994 , Y10T428/249994 , Y10T428/252 , Y10T428/254 , Y10T428/287 , Y10T428/31511 , Y10T428/31663
摘要： The present invention provides a resin composition which can produce a multilayer printed wiring board not causing peeling and crack in a thermal shock test such as a cooling/heating cycle, and having high heat resistance and low-thermal expansion characteristics, when the resin composition is used for an insulating layer of the multilayer printed wiring board; and also an insulating sheet provided on a base, a prepreg, a multilayer printed wiring board and a semiconductor device using thereof. The resin composition is used for forming an insulating layer of the multilayer printed wiring board, wherein a surface roughness parameter Rvk value of the insulating layer is from 0.1 μm to 0.8 μm, measured after the insulating layer being formed with the resin composition, and subject to roughening treatment.
摘要翻译：本发明提供一种树脂组合物，其可以在树脂组合物为热塑性树脂组合物为热塑性树脂组合物时，能够制造在冷热循环等热冲击试验中不产生剥离和裂纹的多层印刷布线板，并且具有高耐热性和低热膨胀特性用于多层印刷线路板的绝缘层; 以及设置在基底上的绝缘片，预浸料，多层印刷线路板和使用其的半导体器件。树脂组合物用于形成多层印刷线路板的绝缘层，其中绝缘层的表面粗糙度参数Rvk值为0.1μm至0.8μm，在用树脂组合物形成绝缘层之后测量，并且对象进行粗糙处理。

9. 发明申请

US20080102391A1 METHOD FOR PREPARING PHOTORECEPTOR, PHOTORECEPTOR PREPARED BY THE METHOD, AND IMAGE FORMING METHOD AND APPARATUS AND PROCESS CARTRIDGE USING THE PHOTORECEPTOR 有权
标题翻译：制备光电子的方法，通过该方法制备的光电子器件和图像形成方法和使用光电元件的装置和工艺盒
公开(公告)号：US20080102391A1
公开(公告)日：2008-05-01
申请号：US11926748
申请日：2007-10-29
申请人： Yoshiki YANAGAWA , Michio Kimura , Nozomu Tamoto , Hiromi Tada , Tetsuya Toshine , Tomoyuki Shimada , Chiaki Tanaka
发明人： Yoshiki YANAGAWA , Michio Kimura , Nozomu Tamoto , Hiromi Tada , Tetsuya Toshine , Tomoyuki Shimada , Chiaki Tanaka
IPC分类号： G03G5/04 , G03G15/00
CPC分类号： G03G5/0546 , G03G5/0525 , G03G5/0532 , G03G5/0614 , G03G5/0616 , G03G5/0629 , G03G5/14734 , G03G5/14791
摘要： A method for preparing a photoreceptor including forming a photosensitive layer overlying an electroconductive substrate; coating a liquid including a radically polymerizable compound to form a protective layer; irradiating the protective layer with light to crosslink the protective layer; and then contacting the protective layer with a fluid, which is selected from the group consisting of supercritical fluids and subcritical fluids and which includes a charge transport material. A photoreceptor prepared by the method. An image forming method, an image forming apparatus and a process cartridge using the photoreceptor.
摘要翻译：一种制备感光体的方法，包括形成覆盖在导电基片上的感光层; 涂布包含自由基聚合性化合物的液体以形成保护层; 用光照射保护层以使保护层交联; 然后使保护层与从超临界流体和亚临界流体组成的组中选择的流体接触并且包括电荷输送材料。通过该方法制备的感光体。图像形成方法，图像形成装置和使用感光体的处理盒。

10. 发明申请

US20080063962A1 ELECTROPHOTOGRAPHIC PHOTOCONDUCTOR, METHOD FOR PRODUCING THE SAME, IMAGE FORMING PROCESS, IMAGE FORMING APPARATUS AND PROCESS CARTRIDGE 有权
标题翻译：电子照相机，其制造方法，图像形成工艺，图像形成装置和工艺盒
公开(公告)号：US20080063962A1
公开(公告)日：2008-03-13
申请号：US11850394
申请日：2007-09-05
申请人： Tetsuya TOSHINE , Chiaki Tanaka , Michio Kimura , Tomoyuki Shimada , Nozomu Tamoto , Yoshiki Yanagawa , Hiromi Tada
发明人： Tetsuya TOSHINE , Chiaki Tanaka , Michio Kimura , Tomoyuki Shimada , Nozomu Tamoto , Yoshiki Yanagawa , Hiromi Tada
IPC分类号： G03G15/02 , G03G5/00
CPC分类号： G03G5/051 , G03G5/0514 , G03G5/0517 , G03G5/0521 , G03G5/0525 , G03G5/0532 , G03G5/0535 , G03G5/0578 , G03G5/14786 , G03G5/14791 , G03G5/14795
摘要： The present invention provides an electrophotographic photoconductor capable of reducing latent electrostatic image stability defects caused by adhesion/adsorption of an electric discharge product formed by a charger in an image forming process, degradation of charge transportability and cleaning defects caused when removing a residual toner. The electrophotographic photoconductor has a conductive substrate, and a photosensitive layer which contains at least a binder, a charge generating material and a charge transporting material and is formed on the substrate, wherein the photosensitive layer contains an injection material composed of at least any one of one wax selected from paraffin waxes, Fisher-Tropsh waxes, polyolefin waxes and a polyorganosiloxane compound in an area from the surface of the photosensitive layer to 50% of the thickness thereof in the thickness direction of the electrophotographic photoconductor, and the content of the injection material is 3% by mass or more to the content of the binder.
摘要翻译：本发明提供了一种电子照相感光体，其能够降低在图像形成过程中由充电器形成的放电产物的附着/吸附引起的静电潜像稳定性缺陷，电荷输送性降低和清除残留调色剂时引起的清洁缺陷。电子照相感光体具有导电性基材和至少含有粘合剂，电荷产生材料和电荷输送材料并形成在基材上的感光层，其中感光层含有由以下的至少一种构成的注入材料：在感光层的表面至电子照相感光体的厚度方向的50％的范围内，选自石蜡，Fisher-Tropsh蜡，聚烯烃蜡和聚有机硅氧烷化合物的一种蜡，以及注射用量材料相对于粘合剂的含量为3质量％以上。

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