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1. 发明授权

US07816103B2 Protein and process for preparing the same 失效
标题翻译：蛋白质及其制备方法
公开(公告)号：US07816103B2
公开(公告)日：2010-10-19
申请号：US11833653
申请日：2007-08-03
申请人： Fusao Makishima , Hiroyuki Takamatsu , Hideo Miki , Shinji Kawai , Michio Kimura , Tomoaki Matsumoto , Mieko Katsuura , Koichi Enomoto , Yusuke Satoh
发明人： Fusao Makishima , Hiroyuki Takamatsu , Hideo Miki , Shinji Kawai , Michio Kimura , Tomoaki Matsumoto , Mieko Katsuura , Koichi Enomoto , Yusuke Satoh
IPC分类号： C12P21/02 , C12N1/21 , C12N15/70 , C07K14/51
CPC分类号： C07K14/51 , A61K38/00 , Y10S514/801
摘要： A protein having amino acid sequence in SEQ ID No.:1 of the Sequence Listing derived from human MP52, and a dimer protein thereof. A homodimer protein described above can be obtained by constructing a plasmid containing DNA coding amino acid sequence in SEQ ID No.:1 of the Sequence Listing with a methionine at the N-terminus, introducing the plasmid into E. coli for transformation, solubilizing inclusion bodies obtained by culturing the transformant, purifying the monomer protein from the solubilized solution, refolding the monomer protein into a dimer protein and purifying the same. The homodimer protein described above is useful as a pharmaceutical composition for treating cartilage and bone diseases.
摘要翻译：衍生自人MP52的序列表的SEQ ID No.1中具有氨基酸序列的蛋白质及其二聚体蛋白质。上述同型二聚体蛋白可以通过在N末端构建含有序列表SEQ ID No.1的DNA编码氨基酸序列的质粒与甲硫氨酸，将质粒导入大肠杆菌进行转化，溶解包合物通过培养转化体获得的物质，从溶解的溶液中纯化单体蛋白质，将单体蛋白质重折叠成二聚体蛋白质并进行纯化。上述同型二聚体蛋白质可用作治疗软骨和骨疾病的药物组合物。

2. 发明授权

US5908747A Mutant gene causing a defect in mitochondrial recombination and a method for its detection 失效
标题翻译：导致线粒体重组缺陷的突变基因及其检测方法
公开(公告)号：US5908747A
公开(公告)日：1999-06-01
申请号：US668448
申请日：1996-06-21
申请人： Takehiko Shibata , Feng Ling , Fusao Makishima
发明人： Takehiko Shibata , Feng Ling , Fusao Makishima
IPC分类号： A61K38/00 , C07K14/395 , C12N15/01 , C12N15/04 , C12Q1/68 , C07H21/04
CPC分类号： C12N15/04 , C07K14/395 , C12N15/01 , A61K38/00
摘要： A multi-step method of detecting a gene in a nuclear chromosome which is involved in mitochondrial recombination is disclosed. The method disclosed includes the steps: a) fusing enucleated .omega..sup.- mitochondrial donor cells expressing a first marker gene with nucleus-containing .omega..sup.+ mitochondrial recipient cells expressing a second marker gene different from the first marker gene to form fused cells wherein the fused cells have one type of mitochondria and b) selecting fused cells that are .omega..sup.+ and express the first and second marker genes to identify those enucleated .omega..sup.- mitochondrial donor cells with a reduced recombination frequency. The method includes the further steps of judging that the gene in a nuclear chromosome of the recipient cells involved in mitochondrial recombination is normal when the mitochondrial recombination frequency is high or that the gene is mutated when the recombination frequency is low. The judging steps permit the detection of a mutant gene in a nuclear chromosome of the recipient cells used for cell fusion. An isolated mhr1 and MHR1 gene coding for a polypeptide is also disclosed.
摘要翻译：公开了一种检测参与线粒体重组的核染色体中的基因的多步法。所公开的方法包括以下步骤：a）将表达第一标记基因的去核的ω-线粒体供体细胞与含有核的含有ω+线粒体受体细胞融合，其表达与第一标记基因不同的第二标记基因以形成融合细胞，其中融合细胞具有一种类型的线粒体，以及b）选择ω+的融合细胞并表达第一和第二标记基因以鉴定具有降低的重组频率的那些去核的ω-线粒体供体细胞。该方法包括以下步骤：当线粒体重组频率高时参与线粒体重组的受体细胞的核染色体中的基因正常，或者当重组频率低时基因突变。判断步骤允许检测用于细胞融合的受体细胞的核染色体中的突变基因。还公开了编码多肽的分离的mhr1和MHR1基因。

3. 发明授权

US07268114B2 Protein and process for preparing the same 失效
标题翻译：蛋白质及其制备方法
公开(公告)号：US07268114B2
公开(公告)日：2007-09-11
申请号：US10365231
申请日：2003-02-12
申请人： Fusao Makishima , Hiroyuki Takamatsu , Hideo Miki , Shinji Kawai , Michio Kimura , Tomoaki Matsumoto , Mieko Katsuura , Koichi Enomoto , Yusuke Satoh
发明人： Fusao Makishima , Hiroyuki Takamatsu , Hideo Miki , Shinji Kawai , Michio Kimura , Tomoaki Matsumoto , Mieko Katsuura , Koichi Enomoto , Yusuke Satoh
IPC分类号： A61K38/18 , C07K14/51 , C12N15/12 , C12N1/21 , C12P21/02 , A61F2/30 , A61F2/28
CPC分类号： C07K14/51 , A61K38/00 , Y10S514/801
摘要： A protein having amino acid sequence in SEQ ID NO: 1 of the Sequence Listing derived from human MP52, and a dimer protein thereof. A homodimer protein described above can be obtained by constructing a plasmid containing DNA coding amino acid sequence in SEQ ID NO: 1 of the Sequence Listing with a methionine at the N-terminus, introducing the plasmid into E. coli for transformation, solubilizing inclusion bodies obtained by culturing the transformant, purifying the monomer protein from the solubilized solution, refolding the monomer protein into a dimer protein and purifying the same. The homodimer protein described above is useful as a pharmaceutical composition for treating cartilage and bone diseases.
摘要翻译：衍生自人MP52的序列表的SEQ ID NO：1中具有氨基酸序列的蛋白质及其二聚体蛋白质。可以通过在N末端构建含有序列表的SEQ ID NO：1的DNA编码氨基酸序列的质粒与甲硫氨酸在N末端引入，将质粒导入大肠杆菌进行转化，从而增溶包涵体通过培养转化体获得，从溶解的溶液中纯化单体蛋白质，将单体蛋白重折叠成二聚体蛋白质并进行纯化。上述同型二聚体蛋白质可用作治疗软骨和骨疾病的药物组合物。

4. 发明申请

US20090325864A1 NOVEL PROTEIN AND PROCESS FOR PREPARING THE SAME 失效
标题翻译：新蛋白及其制备方法
公开(公告)号：US20090325864A1
公开(公告)日：2009-12-31
申请号：US11833653
申请日：2007-08-03
申请人： Fusao MAKISHIMA , Hiroyuki TAKAMATSU , Hideo MIKI , Shinji KAWAI , Michio KIMURA , Tomoaki MATSUMOTO , Mieko KATSUURA , Koichi ENOMOTO , Yusuke SATOH
发明人： Fusao MAKISHIMA , Hiroyuki TAKAMATSU , Hideo MIKI , Shinji KAWAI , Michio KIMURA , Tomoaki MATSUMOTO , Mieko KATSUURA , Koichi ENOMOTO , Yusuke SATOH
IPC分类号： A61K38/18 , C07K14/51 , C12P21/02 , C07H21/04
CPC分类号： C07K14/51 , A61K38/00 , Y10S514/801
摘要： A protein having amino acid sequence in SEQ ID No.:1 of the Sequence Listing derived from human MP52, and a dimer protein thereof. A homodimer protein described above can be obtained by constructing a plasmid containing DNA coding amino acid sequence in SEQ ID No.:1 of the Sequence Listing with a methionine at the N-terminus, introducing the plasmid into E. coli for transformation, solubilizing inclusion bodies obtained by culturing the transformant, purifying the monomer protein from the solubilized solution, refolding the monomer protein into a dimer protein and purifying the same.The homodimer protein described above is useful as a pharmaceutical composition for treating cartilage and bone diseases.
摘要翻译：衍生自人MP52的序列表的SEQ ID No.1中具有氨基酸序列的蛋白质及其二聚体蛋白质。上述同型二聚体蛋白可以通过在N末端构建含有序列表SEQ ID No.1的DNA编码氨基酸序列的质粒与甲硫氨酸，将质粒导入大肠杆菌进行转化，溶解包合物通过培养转化体获得的物质，从溶解的溶液中纯化单体蛋白质，将单体蛋白质重折叠成二聚体蛋白质并进行纯化。上述同型二聚体蛋白质可用作治疗软骨和骨疾病的药物组合物。

5. 发明授权

US07235527B2 Protein and process for producing the same 失效
标题翻译：蛋白质及其生产方法
公开(公告)号：US07235527B2
公开(公告)日：2007-06-26
申请号：US08945459
申请日：1996-04-19
申请人： Fusao Makishima , Hiroyuki Takamatsu , Hideo Miki , Shinji Kawai , Michio Kimura , Tomoaki Matsumoto , Mieko Katsuura , Koichi Enomoto , Yusuke Satoh
发明人： Fusao Makishima , Hiroyuki Takamatsu , Hideo Miki , Shinji Kawai , Michio Kimura , Tomoaki Matsumoto , Mieko Katsuura , Koichi Enomoto , Yusuke Satoh
IPC分类号： A61K38/18 , A61K38/39 , A61K9/14 , C07K14/51
CPC分类号： C07K14/51 , A61K38/00 , Y10S514/801
摘要： A protein consisting of the amino acid sequence in SEQ ID No: 1 derived from human MP52 and a homodimer protein thereof useful for treating cartilage and/or bone diseases and a process for its preparation.
摘要翻译：由源自人MP52的SEQ ID No：1的氨基酸序列和用于治疗软骨和/或骨疾病的同二聚体蛋白质组成的蛋白质及其制备方法。

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