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1. 发明授权

US08481254B2 Substrate probe for use in measuring enzyme activity 有权
标题翻译：用于测量酶活性的底物探针
公开(公告)号：US08481254B2
公开(公告)日：2013-07-09
申请号：US12675511
申请日：2008-09-08
申请人： Fumio Yamauchi , Kimihiro Yoshimura , Katsuaki Kuge , Tetsuya Yano , Masahiro Shirakawa , Yasuhiro Aoyama , Shinsuke Sando
发明人： Fumio Yamauchi , Kimihiro Yoshimura , Katsuaki Kuge , Tetsuya Yano , Masahiro Shirakawa , Yasuhiro Aoyama , Shinsuke Sando
IPC分类号： C12Q1/00
CPC分类号： C12Q1/54 , C12Q1/32 , C12Q1/34 , G01N24/088 , G01R33/281 , G01R33/4633 , G01R33/465 , G01R33/483 , G01R33/5601 , Y02P20/582
摘要： Substrate probe capable of detecting enzyme activity with high accuracy and a method for detecting the enzyme activity by a multi nuclear magnetic resonance method using the substrate probe. Multi-dimensional nuclear magnetic resonance is performed by using a substrate probe, which is used for measuring enzyme activity by a multi-dimensional nuclear magnetic resonance method and characterized by containing a enzyme recognition site that is selectively recognized by an active-state enzyme, as at least one constitutional unit, and a group to which at least three nuclear magnetic resonance active nuclei each having a nuclear spin and a different resonance frequency are connected, being present specifically to the enzyme recognition, thereby detecting presence of the substrate probe and the enzyme activity. Alternatively, imaging of the enzyme activity is performed by a multi-dimensional nuclear resonance imaging method.
摘要翻译：能够高精度地检测酶活性的底物探针和使用底物探针的多核磁共振法检测酶活性的方法。通过使用用于通过多维核磁共振法测定酶活性的底物探针进行多维核磁共振，其特征在于含有被活性酶选择性识别的酶识别位点作为至少一个结构单元和至少三个具有核自旋和不同共振频率的核磁共振活性核连接的基团，其特异性地存在于酶识别中，从而检测底物探针和酶的存在活动。或者，通过多维核共振成像方法进行酶活性的成像。

2. 发明申请

US20100248279A1 SUBSTRATE PROBE, ENZYME-ACTIVITY DETECTION METHOD BY A MULTI-DIMENSIONAL NUCLEAR MAGNETIC RESONANCE METHOD AND ENZYME-ACTIVITY IMAGING METHOD 有权
标题翻译：基质探针，酶活性检测方法多维核磁共振法和酶活性成像方法
公开(公告)号：US20100248279A1
公开(公告)日：2010-09-30
申请号：US12675511
申请日：2008-09-08
申请人： Fumio Yamauchi , Kimihiro Yoshimura , Katsuaki Kuge , Tetsuya Yano , Masahiro Shirakawa , Yasuhiro Aoyama , Shinsuke Sando
发明人： Fumio Yamauchi , Kimihiro Yoshimura , Katsuaki Kuge , Tetsuya Yano , Masahiro Shirakawa , Yasuhiro Aoyama , Shinsuke Sando
IPC分类号： C12Q1/54 , C12Q1/32 , C12Q1/37
CPC分类号： C12Q1/54 , C12Q1/32 , C12Q1/34 , G01N24/088 , G01R33/281 , G01R33/4633 , G01R33/465 , G01R33/483 , G01R33/5601 , Y02P20/582
摘要： Substrate probe capable of detecting enzyme activity with high accuracy and a method for detecting the enzyme activity by a multi nuclear magnetic resonance method using the substrate probe. Multi-dimensional nuclear magnetic resonance is performed by using a substrate probe, which is used for measuring enzyme activity by a multi-dimensional nuclear magnetic resonance method and characterized by containing a enzyme recognition site that is selectively recognized by an active-state enzyme, as at least one constitutional unit, and a group to which at least three nuclear magnetic resonance active nuclei each having a nuclear spin and a different resonance frequency are connected, being present specifically to the enzyme recognition, thereby detecting presence of the substrate probe and the enzyme activity. Alternatively, imaging of the enzyme activity is performed by a multi-dimensional nuclear resonance imaging method.
摘要翻译：能够高精度地检测酶活性的底物探针和使用底物探针的多核磁共振法检测酶活性的方法。通过使用用于通过多维核磁共振法测定酶活性的底物探针进行多维核磁共振，其特征在于含有被活性酶选择性识别的酶识别位点作为至少一个结构单元和至少三个具有核自旋和不同共振频率的核磁共振活性核连接的基团，其特异性地存在于酶识别中，从而检测底物探针和酶的存在活动。或者，通过多维核共振成像方法进行酶活性的成像。

3. 发明申请

US20080124706A1 Nucleic acid probe, nucleic acid chip, method for detecting target nucleic acid, method for screening drug, apparatus for detecting target nucleic acid, and, gene diagnosis method 审中-公开
标题翻译：核酸探针，核酸芯片，靶核酸检测方法，药物筛选方法，靶核酸检测装置，基因诊断方法
公开(公告)号：US20080124706A1
公开(公告)日：2008-05-29
申请号：US11384858
申请日：2006-03-21
申请人： Yasuhiro Aoyama , Shinsuke Sando , Toshinori Sasaki , Atsushi Narita
发明人： Yasuhiro Aoyama , Shinsuke Sando , Toshinori Sasaki , Atsushi Narita
IPC分类号： C12Q1/68 , C07H21/04 , C12M1/00
CPC分类号： C12Q1/683 , C12Q2525/301 , C12Q2525/161 , C12Q2521/337
摘要： The nucleic acid probe of the invention is a nucleic acid probe for detecting a trace amount of target nucleic acid highly sensitively, highly accurately, and highly quickly, and is designed so that the nucleic acid probe undergoes a conformational change after hybridization to the target nucleic acid and that the nucleic acid probe, which underwent the conformational change, has a decreased binding strength of hybridization and dissociates from the target nucleic acid, where the conformational change comprises forming a self-cleaving nucleic acid enzyme intramolecularly and the self-cleaving nucleic acid enzyme has cleavage activity toward a cleavage portion within the nucleic acid probe's own molecule.
摘要翻译：本发明的核酸探针是用于高灵敏度，高度精确和高度快速检测微量靶核酸的核酸探针，其设计使得核酸探针在与靶核酸杂交后经历构象变化并且经历构象变化的核酸探针具有降低的杂交结合强度并与靶核酸解离，其中构象变化包括分子内形成自切割核酸酶，并且自切割核酸酶对核酸探针自身分子内的切割部分具有切割活性。

4. 发明授权

US07351531B2 Molecules capable of binding to telomere and the like and method with the use of the same 失效
标题翻译：能够结合端粒等的分子及其使用方法
公开(公告)号：US07351531B2
公开(公告)日：2008-04-01
申请号：US10333152
申请日：2001-07-17
申请人： Isao Saito , Kazuhiko Nakatani , Shinsuke Sando
发明人： Isao Saito , Kazuhiko Nakatani , Shinsuke Sando
IPC分类号： C12Q1/68 , C07H21/00 , C07H21/02
CPC分类号： C07D519/00 , A61K31/4375
摘要： A method of forming a self-connective structure (a hairpin structure, a four-stranded structure, etc.) of a single-stranded oligonucleotide chain by forming a mimetic base pair of a mismatch base pair failing to form any normal base pair among base pairs of the single-stranded oligonucleotide chain with the use of a compounds represented by the following general formula (I): A-L-B (I) wherein A represents a chemical structural moiety capable of forming a pair with one base of a base pair failing to form any normal base pair; B represents a chemical structural moiety capable of forming a pair with the other base of the base pair failing to form any normal base pair; and L represents a linker structure by which the chemical structures A and B are linked to each other; and a method of inhibiting the activity of enzyme according to the previous method.
摘要翻译：通过形成不能在碱基中形成任何正常碱基对的错配碱基对的模拟碱基对，形成单链寡核苷酸链的自连接结构（发夹结构，四链结构等）的方法使用由以下通式（I）表示的化合物的单链寡核苷酸链对：ALB（I）其中A表示能够与未成形的碱基对的一个碱基形成一对的化学结构部分任何正常的碱基对; B表示能够与碱基对的另一个碱基形成一对而不能形成任何正常碱基对的化学结构部分; L表示化学结构A和B彼此连接的连接体结构; 以及根据前述方法抑制酶的活性的方法。

5. 发明授权

US06607890B1 Mismatch-recognizing molecules 失效
标题翻译：不匹配识别分子
公开(公告)号：US06607890B1
公开(公告)日：2003-08-19
申请号：US09889976
申请日：2001-07-24
申请人： Kazuhiko Nakatani , Isao Saito , Shinsuke Sando
发明人： Kazuhiko Nakatani , Isao Saito , Shinsuke Sando
IPC分类号： C12Q168
CPC分类号： C12Q1/6832 , C12Q2527/125 , C12Q2527/107
摘要： A method whereby a mismatched base pair in a nucleic acid such as DNA or RNA can be conveniently and highly sensitively detected; and detection reagents therefor. A method comprising, in a base pair failing to form a normal base pair, forming a mimetic base pair with the use of a mismatch-recognizing molecule represented by the following general formula (I): A—L—B (I) wherein A represents a chemical structure moiety capable of forming a pair with one of the bases of the base pair failing to form a normal base pair; B represents a chemical structure moiety capable of forming a pair with the other of the bases of the base pair failing to form a normal base pair; and L represents a linker structure whereby the chemical structure moieties A and B are linked to each other; and measuring the formation of the above-described mimetic base pair to thereby detect and identify the base pair failing to form a normal base pair; kits therefor; reagents for detecting a mismatch; and a method of detecting an abnormality in the base sequence of a gene.
摘要翻译：可以方便，高度敏感地检测核酸如DNA或RNA中的错配碱基对的方法; 及其检测试剂。在不能形成正常碱基对的碱基对中，使用由以下通式（I）表示的错配识别分子形成模拟碱基对：ALB（I）其中A表示化学结构能够与碱基对的碱基之一形成一对的部分未能形成正常的碱基对; B表示能够与碱基对的另一个碱基形成正常碱基对的化学结构部分; L表示连接结构，其中化学结构部分A和B彼此连接; 并测量上述模拟碱基对的形成，从而检测和鉴定不能形成正常碱基对的碱基对; 套装; 用于检测错配的试剂; 以及检测基因的碱基序列异常的方法。

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