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1. 发明授权

US08481254B2 Substrate probe for use in measuring enzyme activity 有权
标题翻译：用于测量酶活性的底物探针
公开(公告)号：US08481254B2
公开(公告)日：2013-07-09
申请号：US12675511
申请日：2008-09-08
申请人： Fumio Yamauchi , Kimihiro Yoshimura , Katsuaki Kuge , Tetsuya Yano , Masahiro Shirakawa , Yasuhiro Aoyama , Shinsuke Sando
发明人： Fumio Yamauchi , Kimihiro Yoshimura , Katsuaki Kuge , Tetsuya Yano , Masahiro Shirakawa , Yasuhiro Aoyama , Shinsuke Sando
IPC分类号： C12Q1/00
CPC分类号： C12Q1/54 , C12Q1/32 , C12Q1/34 , G01N24/088 , G01R33/281 , G01R33/4633 , G01R33/465 , G01R33/483 , G01R33/5601 , Y02P20/582
摘要： Substrate probe capable of detecting enzyme activity with high accuracy and a method for detecting the enzyme activity by a multi nuclear magnetic resonance method using the substrate probe. Multi-dimensional nuclear magnetic resonance is performed by using a substrate probe, which is used for measuring enzyme activity by a multi-dimensional nuclear magnetic resonance method and characterized by containing a enzyme recognition site that is selectively recognized by an active-state enzyme, as at least one constitutional unit, and a group to which at least three nuclear magnetic resonance active nuclei each having a nuclear spin and a different resonance frequency are connected, being present specifically to the enzyme recognition, thereby detecting presence of the substrate probe and the enzyme activity. Alternatively, imaging of the enzyme activity is performed by a multi-dimensional nuclear resonance imaging method.
摘要翻译：能够高精度地检测酶活性的底物探针和使用底物探针的多核磁共振法检测酶活性的方法。通过使用用于通过多维核磁共振法测定酶活性的底物探针进行多维核磁共振，其特征在于含有被活性酶选择性识别的酶识别位点作为至少一个结构单元和至少三个具有核自旋和不同共振频率的核磁共振活性核连接的基团，其特异性地存在于酶识别中，从而检测底物探针和酶的存在活动。或者，通过多维核共振成像方法进行酶活性的成像。

2. 发明申请

US20100248279A1 SUBSTRATE PROBE, ENZYME-ACTIVITY DETECTION METHOD BY A MULTI-DIMENSIONAL NUCLEAR MAGNETIC RESONANCE METHOD AND ENZYME-ACTIVITY IMAGING METHOD 有权
标题翻译：基质探针，酶活性检测方法多维核磁共振法和酶活性成像方法
公开(公告)号：US20100248279A1
公开(公告)日：2010-09-30
申请号：US12675511
申请日：2008-09-08
申请人： Fumio Yamauchi , Kimihiro Yoshimura , Katsuaki Kuge , Tetsuya Yano , Masahiro Shirakawa , Yasuhiro Aoyama , Shinsuke Sando
发明人： Fumio Yamauchi , Kimihiro Yoshimura , Katsuaki Kuge , Tetsuya Yano , Masahiro Shirakawa , Yasuhiro Aoyama , Shinsuke Sando
IPC分类号： C12Q1/54 , C12Q1/32 , C12Q1/37
CPC分类号： C12Q1/54 , C12Q1/32 , C12Q1/34 , G01N24/088 , G01R33/281 , G01R33/4633 , G01R33/465 , G01R33/483 , G01R33/5601 , Y02P20/582
摘要： Substrate probe capable of detecting enzyme activity with high accuracy and a method for detecting the enzyme activity by a multi nuclear magnetic resonance method using the substrate probe. Multi-dimensional nuclear magnetic resonance is performed by using a substrate probe, which is used for measuring enzyme activity by a multi-dimensional nuclear magnetic resonance method and characterized by containing a enzyme recognition site that is selectively recognized by an active-state enzyme, as at least one constitutional unit, and a group to which at least three nuclear magnetic resonance active nuclei each having a nuclear spin and a different resonance frequency are connected, being present specifically to the enzyme recognition, thereby detecting presence of the substrate probe and the enzyme activity. Alternatively, imaging of the enzyme activity is performed by a multi-dimensional nuclear resonance imaging method.
摘要翻译：能够高精度地检测酶活性的底物探针和使用底物探针的多核磁共振法检测酶活性的方法。通过使用用于通过多维核磁共振法测定酶活性的底物探针进行多维核磁共振，其特征在于含有被活性酶选择性识别的酶识别位点作为至少一个结构单元和至少三个具有核自旋和不同共振频率的核磁共振活性核连接的基团，其特异性地存在于酶识别中，从而检测底物探针和酶的存在活动。或者，通过多维核共振成像方法进行酶活性的成像。

3. 发明申请

US20100225314A1 NUCLEAR MAGNETIC RESONANCE MEASURING METHOD 有权
标题翻译：核磁共振测量方法
公开(公告)号：US20100225314A1
公开(公告)日：2010-09-09
申请号：US12676239
申请日：2008-09-08
申请人： Katsuaki Kuge , Kimihiro Yoshimura , Fumio Yamauchi , Tetsuya Yano , Masahiro Shirakawa , Yasuhiro Aoyama
发明人： Katsuaki Kuge , Kimihiro Yoshimura , Fumio Yamauchi , Tetsuya Yano , Masahiro Shirakawa , Yasuhiro Aoyama
IPC分类号： G01R33/44
CPC分类号： G01R33/485 , G01R33/4608 , G01R33/4633 , G01R33/4833 , G01R33/5605 , G01R33/5607
摘要： The present invention provides a method for measuring nuclear magnetic resonance that employs a compound in which a plurality of nuclei is labeled with isotopes as a probe agent, highly selectively and highly sensitively obtains a nuclear magnetic resonance signal of the above described probe agent, and can attach a spatial positional information to the above described nuclear magnetic resonance signal, and an apparatus therefore.
摘要翻译：本发明提供一种测定核磁共振的方法，其使用多个核被同位素标记的化合物作为探针剂，高度选择性且高度灵敏地获得上述探针剂的核磁共振信号，并且可以将空间位置信息附加到上述核磁共振信号，因此装置。

4. 发明授权

US08773126B2 Nuclear magnetic resonance measuring method using an isotope-labeled compound 有权
标题翻译：使用同位素标记化合物的核磁共振测量方法
公开(公告)号：US08773126B2
公开(公告)日：2014-07-08
申请号：US12676239
申请日：2008-09-08
申请人： Katsuaki Kuge , Kimihiro Yoshimura , Fumio Yamauchi , Tetsuya Yano , Masahiro Shirakawa , Yasuhiro Aoyama
发明人： Katsuaki Kuge , Kimihiro Yoshimura , Fumio Yamauchi , Tetsuya Yano , Masahiro Shirakawa , Yasuhiro Aoyama
IPC分类号： G01V3/00 , G01R33/485 , G01R33/46 , G01R33/483 , G01R33/56
CPC分类号： G01R33/485 , G01R33/4608 , G01R33/4633 , G01R33/4833 , G01R33/5605 , G01R33/5607
摘要： The present invention provides a method for measuring nuclear magnetic resonance that employs a compound in which a plurality of nuclei is labeled with isotopes as a probe agent, highly selectively and highly sensitively obtains a nuclear magnetic resonance signal of the above described probe agent, and can attach a spatial positional information to the above described nuclear magnetic resonance signal, and an apparatus therefore.
摘要翻译：本发明提供一种测定核磁共振的方法，其使用多个核被同位素标记的化合物作为探针剂，高度选择性且高度灵敏地获得上述探针剂的核磁共振信号，并且可以将空间位置信息附加到上述核磁共振信号，因此装置。

5. 发明申请

US20100247444A1 COMPOUND, DIAGNOSTIC AGENT, NUCLEAR MAGNETIC RESONANCE ANALYSIS METHOD, NUCLEAR MAGNETIC RESONANCE IMAGING METHOD, MASS SPECTROMETRY METHOD AND MASS SPECTROMETRY IMAGING METHOD 审中-公开
标题翻译：化合物，诊断剂，核磁共振分析方法，核磁共振成像方法，质谱法和质谱法成像方法
公开(公告)号：US20100247444A1
公开(公告)日：2010-09-30
申请号：US12675581
申请日：2008-09-08
申请人： Kimihiro Yoshimura , Fumio Yamauchi , Katsuaki Kuge , Tetsuya Yano , Masahiro Shirakawa , Yasuhiro Aoyama
发明人： Kimihiro Yoshimura , Fumio Yamauchi , Katsuaki Kuge , Tetsuya Yano , Masahiro Shirakawa , Yasuhiro Aoyama
IPC分类号： A61K49/10 , C07F9/02 , A61P43/00
CPC分类号： C07C215/40 , A61K49/10 , C07B2200/05 , C07C219/06 , C07F9/091 , C07F9/106 , G01N24/08 , G01R33/4608 , G01R33/5601 , G01R33/5605
摘要： To produce a labeled compound for a selected biological substance not using a radioisotope atom which has a risk of exposure to radioactivity and limitation on handling time but using a stable isotope atom; and that the labeled compound can be measured with good sensitivity separably from naturally occurring compounds of the selected biological substance which are substituted with the stable isotope atom. Choline as a biological substance is labeled by substituting the nitrogen atom of the quaternary ammonium group and all the carbon atoms of the methyl group attached to the nitrogen atom with respective isotopes 15N and 13C and used as a diagnostic agent.
摘要翻译：为了生产不使用具有暴露于放射性和限制处理时间但使用稳定同位素原子的放射性同位素原子的选定生物物质的标记化合物; 并且可以以与稳定同位素原子取代的所选择的生物物质的天然存在的化合物分离地具有良好的灵敏度来测量标记的化合物。胆碱作为生物物质通过用各自的同位素15N和13C替代季铵基团的氮原子和与氮原子连接的甲基的所有碳原子并用作诊断剂进行标记。

6. 发明申请

US20100112601A1 NOVEL MONOCLONAL ANTIBODY AND USE OF THE SAME 审中-公开
标题翻译：新型单克隆抗体及其用途
公开(公告)号：US20100112601A1
公开(公告)日：2010-05-06
申请号：US12309998
申请日：2007-08-07
申请人： Masahiro Shirakawa , Hiroshi Inooka
发明人： Masahiro Shirakawa , Hiroshi Inooka
IPC分类号： G01N33/567 , C07K16/00
CPC分类号： C07K16/28 , C07K2317/55 , C07K2317/76 , C07K2317/92 , G01N33/56966 , G01N33/74 , G01N2333/726
摘要： The present invention provides an anti-PAC1 monoclonal antibody capable of recognizing a PAC1 having a native structure, a PAC1 activity regulator (in particular, activity inhibitor) containing the antibody, a prophylactic/therapeutic agent for a disease associated with accentuation of a bioactivity of PAC1, containing the antibody, a diagnostic reagent for a disease associated with an abnormality of PAC1 activity, containing the antibody, and a screening method for a substance that regulates the expression of PAC1, using the antibody and a PAC1-expressing cell.
摘要翻译：本发明提供能够识别具有天然结构的PAC1的抗PAC1单克隆抗体，含有抗体的PAC1活性调节剂（特别是活性抑制剂），与增加生物活性相关的疾病的预防/治疗剂含有抗体的PAC1，含有抗体的PAC1活性异常的疾病的诊断试剂，以及使用抗体和PAC1表达细胞调节PAC1的表达的物质的筛选方法。

7. 发明授权

US07400144B2 Magnetic resonance force microscope 失效
标题翻译：磁共振力显微镜
公开(公告)号：US07400144B2
公开(公告)日：2008-07-15
申请号：US11712840
申请日：2007-03-01
申请人： Shigenori Tsuji , Yohsuke Yoshinari , Masahiro Shirakawa , Tetsuro Kobubo
发明人： Shigenori Tsuji , Yohsuke Yoshinari , Masahiro Shirakawa , Tetsuro Kobubo
IPC分类号： G01V3/00 , G01B5/28
CPC分类号： G01Q60/08 , G01Q60/52
摘要： A magnetic resonance force microscope (MRFM) generator for producing an RF magnetic field uniformly over the whole of a sample. A cantilever with magnetic probe tip is self-excited. Under this condition, spins in the sample are controlled to produce a magnetic resonance force. A frequency demodulator measures the resonant frequency of the cantilever from the output detection signal from a cantilever displacement-measuring instrument based on the magnetic resonance force.
摘要翻译：一种用于在整个样品上均匀地产生RF磁场的磁共振力显微镜（MRFM）发生器。具有磁性探针尖端的悬臂是自激的。在这种条件下，控制样品中的自旋以产生磁共振力。频率解调器基于磁共振力从悬臂位移测量仪器的输出检测信号测量悬臂的谐振频率。

8. 发明申请

US20070216412A1 Magnetic resonance force microscope 失效
标题翻译：磁共振力显微镜
公开(公告)号：US20070216412A1
公开(公告)日：2007-09-20
申请号：US11712840
申请日：2007-03-01
申请人： Shigenori Tsuji , Yohsuke Yoshinari , Masahiro Shirakawa , Tetsuro Kobubo
发明人： Shigenori Tsuji , Yohsuke Yoshinari , Masahiro Shirakawa , Tetsuro Kobubo
IPC分类号： G01V3/00
CPC分类号： G01Q60/08 , G01Q60/52
摘要： A magnetic resonance force microscope (MRFM) generator for producing an RF magnetic field uniformly over the whole of a sample. A cantilever with magnetic probe tip is self-excited. Under this condition, spins in the sample are controlled to produce a magnetic resonance force. A frequency demodulator measures the resonant frequency of the cantilever from the output detection signal from a cantilever displacement-measuring instrument based on the magnetic resonance force.
摘要翻译：一种用于在整个样品上均匀地产生RF磁场的磁共振力显微镜（MRFM）发生器。具有磁性探针尖端的悬臂是自激的。在这种条件下，控制样品中的自旋以产生磁共振力。频率解调器基于磁共振力从悬臂位移测量仪器的输出检测信号测量悬臂的谐振频率。

9. 发明申请

US20050244822A1 Method of monitoring gene expression 审中-公开
标题翻译：监测基因表达的方法
公开(公告)号：US20050244822A1
公开(公告)日：2005-11-03
申请号：US10501039
申请日：2003-01-09
申请人： Tetsuro Kokubo , Masahiro Shirakawa , Jeremy Tame
发明人： Tetsuro Kokubo , Masahiro Shirakawa , Jeremy Tame
IPC分类号： G01R33/465 , C12N15/09 , C12Q1/68 , G01N24/08 , G01N33/15 , G01N33/483 , G01N33/50
CPC分类号： C12Q1/6809 , C12Q1/6897 , G01N24/08 , G01R33/465 , C12Q2565/633
摘要： The present invention provides a technique by which highly detailed gene expression profiling data for analyzing the gene function can be obtained safely and conveniently. It is a versatile non-destructive and high-resolution visualizing technique by which expression levels of genes can be monitored in real time, and which can be applied in vivo and to deep tissues. For instance, plasmids in which molecules that vary the NMR signal and can be quantified by PHM genes are incorporated downstream from promoters responding to the condition of medium, external stimuli and the like, are introduced into the cells. The level of polyphosphate in deep tissues and the like can then be quantified by 31P-NMR, allowing expression profiling of the promoter, both non-destructively and in real time.
摘要翻译：本发明提供了可以安全，方便地获得用于分析基因功能的高度详细的基因表达谱分析数据的技术。它是一种多功能的非破坏性和高分辨率可视化技术，可以实时监测基因的表达水平，并且可以在体内和深层组织中应用。例如，将其中改变NMR信号并且可以通过PHM基因定量的分子的质粒引入到响应于培养基，外部刺激等条件的启动子的下游引入细胞中。深层组织中的多磷酸盐水平等可以通过31-P-NMR进行定量，从而可以非破坏性地和实时地进行启动子的表达谱分析。

10. 发明授权

US5961015A Umbrella support attachment for bicycle 失效
标题翻译：伞支撑附件为自行车
公开(公告)号：US5961015A
公开(公告)日：1999-10-05
申请号：US903006
申请日：1997-07-30
申请人： Masahiro Shirakawa
发明人： Masahiro Shirakawa
IPC分类号： B62J11/00
CPC分类号： B62J11/00 , Y10S224/915
摘要： An umbrella support attachment for a bicycle is provided which comprises a distal portion support member including a fastening portion to be attached to a seat stay of the bicycle and a support portion for supporting a distal portion of the umbrella, and a proximal portion support member including a fastening portion to be attached to a frame bar of the bicycle and a clipping portion for supporting a proximal portion of the umbrella. The umbrella support attachment is effective to carry an elongate object such as an umbrella on a bicycle without interfering with the operation of the bicycle and smooth movement of movable components of the bicycle.
摘要翻译：提供了一种用于自行车的伞形支撑附件，其包括远侧部分支撑构件，所述远端部分支撑构件包括附接到自行车的座椅支撑件的紧固部分和用于支撑伞的远侧部分的支撑部分，以及近端部分支撑构件，固定在自行车的框架杆上的紧固部分和用于支撑伞的近端部分的夹持部分。伞形支撑附件有效地将诸如伞的细长物体携带在自行车上，而不会干扰自行车的操作并且使自行车的可移动部件平滑地移动。

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