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1. 发明授权

US07179601B2 Methods of identifying plant disease-resistance genes 失效
标题翻译：识别植物抗病基因的方法
公开(公告)号：US07179601B2
公开(公告)日：2007-02-20
申请号：US10613472
申请日：2003-07-02
申请人： Frederick M. Ausubel , Brian J. Staskawicz , Andrew F. Bent , Douglas Dahlbeck , Fumiaki Katagiri , Barbara N. Kunkel , Michael Nicholas Mindrinos , Guo-Liang Yu , Barbara Baker , Jeffrey Ellis , John Salmeron
发明人： Frederick M. Ausubel , Brian J. Staskawicz , Andrew F. Bent , Douglas Dahlbeck , Fumiaki Katagiri , Barbara N. Kunkel , Michael Nicholas Mindrinos , Guo-Liang Yu , Barbara Baker , Jeffrey Ellis , John Salmeron
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895 , C07K14/415 , C12N15/8281 , C12Q2600/13 , C12Q2600/156
摘要： The invention relates to a method of identifying a plant disease-resistance gene that includes the steps of (a) providing a plant tissue sample; (b)introducing by biolistic transformation into the plant tissue sample a candidate plant disease-resistance gene; (c) expressing the candidate plant disease-resistance gene within the plant tissue sample; and (d) determining whether the plant tissue sample exhibits a disease-resistance response, whereby a response identifies a plant disease-resistance gene.
摘要翻译：本发明涉及鉴定植物病害抗性基因的方法，其包括以下步骤：（a）提供植物组织样品; （b）通过生物弹性转化将候选植物抗病基因引入植物组织样品中; （c）在植物组织样品内表达候选植物病害抗性基因; 和（d）确定植物组织样品是否表现出抗病性反应，由此响应识别植物病害抗性基因。

2. 发明授权

US06262248B1 RPS gene family, primers, probes, and detection methods 有权
标题翻译： RPS基因家族，引物，探针和检测方法
公开(公告)号：US06262248B1
公开(公告)日：2001-07-17
申请号：US09301085
申请日：1999-04-28
申请人： Frederick M. Ausubel , Brian J. Staskawicz , Andrew F. Bent , Douglas Dahlbeck , Fumiaki Katagiri , Barbara N. Kunkel , Michael Nicholas Mindrinos , Guo-Liang Yu , Barbara Baker , Jeffrey Ellis , John Salmeron
发明人： Frederick M. Ausubel , Brian J. Staskawicz , Andrew F. Bent , Douglas Dahlbeck , Fumiaki Katagiri , Barbara N. Kunkel , Michael Nicholas Mindrinos , Guo-Liang Yu , Barbara Baker , Jeffrey Ellis , John Salmeron
IPC分类号： C07A2104
CPC分类号： C12Q1/6895 , C07K14/415 , C12N15/8281 , C12Q2600/13 , C12Q2600/156
摘要： Disclosed is substantially pure DNA encoding an Arabidopsis thaliana Rps2 polypeptide; substantially pure Rps2 polypeptide; and methods of using such DNA to express the Rps2 polypeptide in plant cells and whole plants to provide, in transgenic plants, disease resistance to pathogens. Also disclosed are conserved regions characteristic of the RPS family and primers and probes for the identification and isolation of additional RPS disease-resistance genes.
摘要翻译：公开了编码拟南芥Rps2多肽的基本上纯的DNA; 基本上纯的Rps2多肽; 以及使用这些DNA在植物细胞和整个植物中表达Rps2多肽的方法，以在转基因植物中提供对病原体的抗病性。还公开了用于鉴定和分离另外的RPS抗病基因的RPS家族特征的保守区和引物和探针。

3. 发明授权

US5981730A RPS gene family, primers, probes, and detection methods 失效
公开(公告)号：US5981730A
公开(公告)日：1999-11-09
申请号：US310912
申请日：1994-09-22
申请人： Frederick M. Ausubel , Brian J. Staskawicz , Fumiaki Katagiri , Barbara Baker , Jeffrey Ellis , John Salmeron
发明人： Frederick M. Ausubel , Brian J. Staskawicz , Fumiaki Katagiri , Barbara Baker , Jeffrey Ellis , John Salmeron
IPC分类号： A01H1/00 , A01H5/00 , C07K14/415 , C07K16/16 , C12N5/10 , C12N15/09 , C12N15/82 , C12P21/02 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6895 , C07K14/415 , C12N15/8281 , C12Q2600/13 , C12Q2600/156
摘要： Disclosed is substantially pure DNA encoding an Arabidopsis thaliana Rps2 polypeptide; substantially pure Rps2 polypeptide; and methods of using such DNA to express the Rps2 polypeptide in plant cells and whole plants to provide, in transgenic plants, disease resistance to pathogens. Also disclosed are conserved regions characteristic of the RPS family and primers and probes for the identification and isolation of additional RPS disease-resistance genes.

4. 发明申请

US20080085835A1 RPS GENE FAMILY, PRIMERS, PROBES AND DETECTION METHODS 审中-公开
标题翻译： RPS基因家族，PRIMERS，PROBES和检测方法
公开(公告)号：US20080085835A1
公开(公告)日：2008-04-10
申请号：US11537495
申请日：2006-09-29
申请人： Frederick M. AUSUBEL , Brian J. Staskawicz , Andrew F. Bent , Douglas Dahlbeck , Fumiaki Katagiri , Barbara N. Kunkel , Michael Nicholas , Guo-Liang Yu , Barbara Baker , Jeffrey Ellis , John Salmeron
发明人： Frederick M. AUSUBEL , Brian J. Staskawicz , Andrew F. Bent , Douglas Dahlbeck , Fumiaki Katagiri , Barbara N. Kunkel , Michael Nicholas , Guo-Liang Yu , Barbara Baker , Jeffrey Ellis , John Salmeron
IPC分类号： C40B20/00 , C07H21/00 , C12Q1/68
CPC分类号： C12Q1/6895 , C07K14/415 , C12N15/8281 , C12Q2600/13 , C12Q2600/156
摘要： Disclosed is substantially pure DNA encoding an Arabidopsis thaliana Rps2 polypeptide, substantially pure Rps2 polyneptide; and methods of using such DNA to express the Rps2 polypeptide in plant cells and whole plants to provide, in transgenic plants, disease resistance to pathogens. Also disclosed are conserved regions characteristic of the RPS family and primers and probes for the identification and isolation of additional RPS disease-resistance genes.
摘要翻译：公开了编码拟南芥Rps 2多肽，基本上纯的Rps 2多肽的基本上纯的DNA; 以及使用这些DNA在植物细胞和整个植物中表达Rps2多肽的方法，以在转基因植物中提供对病原体的抗病性。还公开了用于鉴定和分离另外的RPS抗病基因的RPS家族特征的保守区和引物和探针。

5. 发明申请

US20070192909A1 Methods for screening for gene specific hybridization polymorphisms (GSHPs) and their use in genetic mapping ane marker development 审中-公开
标题翻译：筛选基因特异性杂交多态性（GSHPs）的方法及其在遗传图谱分析中的应用
公开(公告)号：US20070192909A1
公开(公告)日：2007-08-16
申请号：US11725636
申请日：2007-03-20
申请人： John Salmeron , Tong Zhu
发明人： John Salmeron , Tong Zhu
IPC分类号： A01H5/00 , C12Q1/68 , G06F19/00
CPC分类号： C12Q1/6827 , G16B25/00
摘要： A method for identification of gene specific hybridization polymorphisms (GSHPs) and their use is presented. The method involves the steps of a) global screening for hybridization polymorphisms using microarray; b) enzyme mediated genome complexity reduction; c) enzyme mediated differential signal amplification and noise reduction; d) data extraction and GSHP identification; and e) use of GSHPs in high throughput screening. The method is useful in a particular application for the fingerprinting of maize exotic germplasm and the identification of new and commercially useful loci therein.
摘要翻译：提出了一种鉴定基因特异性杂交多态性（GSHPs）及其应用的方法。该方法包括以下步骤：a）使用微阵列全面筛选杂交多态性; b）酶介导的基因组复杂性降低; c）酶介导的差异信号放大和降噪; d）数据提取和GSHP鉴定; 和e）在高通量筛选中使用GSHPs。该方法在特定应用中用于玉米异种种质的指纹识别和其中新的和商业上有用的基因座的鉴定。

6. 发明授权

US06956115B2 Nucleic acid molecules from rice encoding RAR1 disease resistance proteins and uses thereof 失效
标题翻译：编码RAR1抗病性蛋白的水稻的核酸分子及其用途
公开(公告)号：US06956115B2
公开(公告)日：2005-10-18
申请号：US10305770
申请日：2002-11-27
申请人： Manuel B. Sainz , John Salmeron
发明人： Manuel B. Sainz , John Salmeron
IPC分类号： C07K14/415 , C12N15/82 , C12N15/29 , A01H5/00 , C12N15/09
CPC分类号： C12N15/8281 , C07K14/415 , C12N15/8279 , C12N15/8282
摘要： The present invention pertains to isolated nucleic acid molecules comprising nucleotide sequences isolated from Oryza sativa that encode RARI proteins involved in disease resistance, and recombinant vectors comprising said nucleotide sequences.
摘要翻译：本发明涉及包含从水稻分离的编码参与抗病性的RARI蛋白的核苷酸序列的分离的核酸分子，以及包含所述核苷酸序列的重组载体。

7. 发明授权

US07098378B2 Transgenic plants compromising nucleic acid molecules encoding RAR1 disease resistance proteins and uses thereof 失效
标题翻译：转基因植物损害编码RAR1抗病性蛋白质的核酸分子及其用途
公开(公告)号：US07098378B2
公开(公告)日：2006-08-29
申请号：US11011906
申请日：2004-12-14
申请人： Manuel B. Sainz , John Salmeron
发明人： Manuel B. Sainz , John Salmeron
IPC分类号： C12N15/09 , C12N15/29 , C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8281 , C07K14/415 , C12N15/8279 , C12N15/8282
摘要： The present invention pertains to transgenic plants comprising nucleic acid molecules comprising nucleotide sequences that encode RAR1 proteins involved in disease resistance, and the RAR1 polypeptides. The invention particularly relates to methods of altering the expressing nucleic acid molecules encoding RAR1 proteins in transgenic plants to alter the level disease resistance, and to transgenic plants, progeny and seed therefrom, having altered enhanced disease resistance. The invention further relates to methods of enhancing expression of R resistance genes, disease resistance signal transduction genes, genes involved in mediating disease resistance or involved in the synthesis of molecules mediating disease resistance. The invention also relates to methods of regulating the expression of other coding sequences of interest by increasing the expression of the nucleic acid molecules of the invention.
摘要翻译：本发明涉及包含核酸分子的转基因植物，所述核酸分子包含编码参与抗病性的RAR1蛋白的核苷酸序列和RAR1多肽。本发明特别涉及改变转基因植物中编码RAR1蛋白的表达核酸分子以改变水平的抗病性的方法，以及改变增强的抗病性的转基因植物，后代和种子。本发明还涉及增强R抗性基因表达，抗病性信号转导基因，介导抗病性的基因或参与抗病性分子合成的方法。本发明还涉及通过增加本发明的核酸分子的表达来调节其它编码序列的表达的方法。

8. 发明申请

US20050132438A1 Novel monocotylednous plant genes and uses thereof 审中-公开
标题翻译：新型单子叶植物基因及其用途
公开(公告)号：US20050132438A1
公开(公告)日：2005-06-16
申请号：US10220665
申请日：2001-03-05
申请人： Helen Wang , John Salmeron , Michael Willits , Kay Lawton
发明人： Helen Wang , John Salmeron , Michael Willits , Kay Lawton
IPC分类号： A01H5/00 , C07K14/415 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/29 , C12N15/82 , C12Q1/68 , A01H1/00 , C07H21/04 , C12N5/04 , C12N9/24
CPC分类号： C12N15/8279 , C07K14/415
摘要： Homologues of the Arabidopsis NIM1 gene, which is involved in the signal transduction cascade leading to systemic acquired resistance (SAR), are isolated from monocotyledonous crops such as Triticum aestivum (wheat) and Oryza sativa (rice). The invention further concerns transformation vectors and processes for expressing the monocotyledonous NIM1 homologues in transgenic plants to increase SAR gene expression and enhance broad spectrum disease resistance
摘要翻译：拟南芥NIM1基因的同源物，其参与导致系统性获得性抗性（SAR）的信号转导级联，从单子叶作物如小麦（Triticum aestivum）（小麦）和水稻（Rice）中分离。本发明还涉及用于在转基因植物中表达单子叶NIM1同源物的转化载体和方法，以增加SAR基因表达并增强广谱抗病性

9. 发明授权

US06646184B2 Trichothecene-resistant transgenic plants 有权
标题翻译：抗逆转录病毒转基因植物
公开(公告)号：US06646184B2
公开(公告)日：2003-11-11
申请号：US10074279
申请日：2002-02-12
申请人： Thomas M. Hohn , Cheryl Peters , John Salmeron
发明人： Thomas M. Hohn , Cheryl Peters , John Salmeron
IPC分类号： C12N1509
CPC分类号： C07H21/04 , C12N9/1029 , C12N15/8209 , C12N15/8242 , C12N15/8274 , C12N15/8282
摘要： The present invention discloses trichothecene-resistant transgenic plants, plant tissues, plant seeds, and plant cells comprising a heterologous polynucleotide encoding a gene product having tricothecene resistance activity that thereby confers trichothecene resistance to the transgenic plants, plant tissues, plant seeds, and plant cells. Trichothecene resistance activity, as used herein, refers to an activity that reduces or inhibits the phytotoxicity of a trichothecene, particularly to a fungus and/or plant. In a particular embodiment, trichothecene resistance activity refers to an activity that transfers an acetate to the C-3 position of a trichothecene such as T-2 toxin, HT-2 toxin, isotrichodermol, diacetoxyscirpenol (“DAS”), 3-deacetylcalonectrin, 3,15-dideacetylcalonectrin, scirpentriol, neosolaniol; 15-acetyldeoxynivalenol, nivalenol, 4-acetylnivalenol (fusarenone-X), 4,15-diacetylnivalenol, 4,7,15-acetylnivalenol, and deoxynivalenol (“DON”) and their various acetylated derivatives. In another particular embodiment, the gene product having trichthecene resistance activity is a 3-O-acetyltransferase from a trichothecene-producing species of Fusarium, such as Fusarium graminearum or Fusarium sporotrichioides.
摘要翻译：本发明公开了头孢霉烯抗性转基因植物，植物组织，植物种子和植物细胞，其包含编码具有三氯生物抗性活性的基因产物的异源多核苷酸，从而赋予转基因植物，植物组织，植物种子和植物细胞的单端孢霉烯抗性。如本文所用，三氯乙炔抗性活性是指减少或抑制单端孢霉烯特别是真菌和/或植物的植物毒性的活性。在一个特定的实施方案中，单端孢霉烯抗性活性是指将乙酸酯转移到单端孢霉烯C-3位的活性，例如T-2毒素，HT-2毒素，异维甲酸，二乙酰氧基甲苯酚（“DAS”），3-脱乙酰基木聚糖， 3,15-二乙酰胆碱，顺丁三醇，新溶胶甘醇; 15-乙酰基过氧化雪腐镰刀菌烯醇，新戊烯醇，4-乙酰基雪腐镰刀菌烯醇（fisarenone-X），4,15-二乙酰基雪腐镰刀菌烯醇，4,7,15-乙酰基雪腐镰刀菌烯醇和脱氧雪腐镰刀菌烯醇（“DON”）及其各种乙酰化衍生物。在另一个具体实施方案中，具有三辛抗性活性的基因产物是来自产生单端孢霉烯的镰刀菌属物种如禾谷镰刀菌（Fusarium graminearum）或孢子镰刀菌（Fusarium sporotrichioides）的3-O-乙酰转移酶。

10. 发明申请

US20050097637A1 Transgenic plants compromising nucleic acid molecules encoding RAR 1 disease resistance proteins and uses thereof 审中-公开
标题翻译：转基因植物损害编码RAR1抗病性蛋白质的核酸分子及其用途
公开(公告)号：US20050097637A1
公开(公告)日：2005-05-05
申请号：US11012974
申请日：2004-12-15
申请人： Manuel Sainz , John Salmeron
发明人： Manuel Sainz , John Salmeron
IPC分类号： C07K14/415 , C12N15/82 , A01H1/00
CPC分类号： C12N15/8281 , C07K14/415 , C12N15/8279 , C12N15/8282
摘要： The present invention pertains to transgenic plants comprising nucleic acid molecules comprising nucleotide sequences that encode RAR1 proteins involved in disease resistance, and the RAR1 polypeptides. The invention particularly relates to methods of altering the expressing nucleic acid molecules encoding RAR1 proteins in transgenic plants to alter the level disease resistance, and to transgenic plants, progeny and seed therefrom, having altered enhanced disease resistance. The invention further relates to methods of enhancing expression of R resistance genes, disease resistance signal transduction genes, genes involved in mediating disease resistance or involved in the synthesis of molecules mediating disease resistance. The invention also relates to methods of regulating the expression of other coding sequences of interest by increasing the expression of the nucleic acid molecules of the invention.
摘要翻译：本发明涉及包含核酸分子的转基因植物，所述核酸分子包含编码参与抗病性的RAR1蛋白的核苷酸序列和RAR1多肽。本发明特别涉及改变转基因植物中编码RAR1蛋白的表达核酸分子以改变水平的抗病性的方法，以及改变增强的抗病性的转基因植物，后代和种子。本发明还涉及增强R抗性基因表达，抗病性信号转导基因，介导抗病性的基因或参与抗病性分子合成的方法。本发明还涉及通过增加本发明的核酸分子的表达来调节其它编码序列的表达的方法。

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