专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06646184B2 Trichothecene-resistant transgenic plants 有权
标题翻译：抗逆转录病毒转基因植物
公开(公告)号：US06646184B2
公开(公告)日：2003-11-11
申请号：US10074279
申请日：2002-02-12
申请人： Thomas M. Hohn , Cheryl Peters , John Salmeron
发明人： Thomas M. Hohn , Cheryl Peters , John Salmeron
IPC分类号： C12N1509
CPC分类号： C07H21/04 , C12N9/1029 , C12N15/8209 , C12N15/8242 , C12N15/8274 , C12N15/8282
摘要： The present invention discloses trichothecene-resistant transgenic plants, plant tissues, plant seeds, and plant cells comprising a heterologous polynucleotide encoding a gene product having tricothecene resistance activity that thereby confers trichothecene resistance to the transgenic plants, plant tissues, plant seeds, and plant cells. Trichothecene resistance activity, as used herein, refers to an activity that reduces or inhibits the phytotoxicity of a trichothecene, particularly to a fungus and/or plant. In a particular embodiment, trichothecene resistance activity refers to an activity that transfers an acetate to the C-3 position of a trichothecene such as T-2 toxin, HT-2 toxin, isotrichodermol, diacetoxyscirpenol (“DAS”), 3-deacetylcalonectrin, 3,15-dideacetylcalonectrin, scirpentriol, neosolaniol; 15-acetyldeoxynivalenol, nivalenol, 4-acetylnivalenol (fusarenone-X), 4,15-diacetylnivalenol, 4,7,15-acetylnivalenol, and deoxynivalenol (“DON”) and their various acetylated derivatives. In another particular embodiment, the gene product having trichthecene resistance activity is a 3-O-acetyltransferase from a trichothecene-producing species of Fusarium, such as Fusarium graminearum or Fusarium sporotrichioides.
摘要翻译：本发明公开了头孢霉烯抗性转基因植物，植物组织，植物种子和植物细胞，其包含编码具有三氯生物抗性活性的基因产物的异源多核苷酸，从而赋予转基因植物，植物组织，植物种子和植物细胞的单端孢霉烯抗性。如本文所用，三氯乙炔抗性活性是指减少或抑制单端孢霉烯特别是真菌和/或植物的植物毒性的活性。在一个特定的实施方案中，单端孢霉烯抗性活性是指将乙酸酯转移到单端孢霉烯C-3位的活性，例如T-2毒素，HT-2毒素，异维甲酸，二乙酰氧基甲苯酚（“DAS”），3-脱乙酰基木聚糖， 3,15-二乙酰胆碱，顺丁三醇，新溶胶甘醇; 15-乙酰基过氧化雪腐镰刀菌烯醇，新戊烯醇，4-乙酰基雪腐镰刀菌烯醇（fisarenone-X），4,15-二乙酰基雪腐镰刀菌烯醇，4,7,15-乙酰基雪腐镰刀菌烯醇和脱氧雪腐镰刀菌烯醇（“DON”）及其各种乙酰化衍生物。在另一个具体实施方案中，具有三辛抗性活性的基因产物是来自产生单端孢霉烯的镰刀菌属物种如禾谷镰刀菌（Fusarium graminearum）或孢子镰刀菌（Fusarium sporotrichioides）的3-O-乙酰转移酶。

2. 发明授权

US07049421B2 Trichothecene-resistant transgenic plants 有权
标题翻译：抗逆转录病毒转基因植物
公开(公告)号：US07049421B2
公开(公告)日：2006-05-23
申请号：US10614954
申请日：2003-07-07
申请人： Thomas M. Hohn , Cheryl Peters , John Salmeron
发明人： Thomas M. Hohn , Cheryl Peters , John Salmeron
IPC分类号： C12N15/09 , C12N15/31 , A01H5/00
CPC分类号： C07H21/04 , C12N9/1029 , C12N15/8209 , C12N15/8242 , C12N15/8274 , C12N15/8282
摘要： The present invention discloses isolated polynucleotides encoding polypeptides having trichothecene 3-O-acetyltransferase activity, recombinant vectors and host cell comprising said polynucleotides.
摘要翻译：本发明公开了编码具有单端孢霉素3-O-乙酰转移酶活性的多肽的分离的多核苷酸，重组载体和包含所述多核苷酸的宿主细胞。

3. 发明授权

US06346655B1 Trichothecne-Resistant transgenic plants 有权
标题翻译：抗逆转录病毒转基因植物
公开(公告)号：US06346655B1
公开(公告)日：2002-02-12
申请号：US09538414
申请日：2000-03-29
申请人： Thomas M. Hohn , Cheryl Peters , John Salmeron
发明人： Thomas M. Hohn , Cheryl Peters , John Salmeron
IPC分类号： C12N1509
CPC分类号： C07H21/04 , C12N9/1029 , C12N15/8209 , C12N15/8242 , C12N15/8274 , C12N15/8282
摘要： The present invention discloses trichothecene-resistant transgenic plants, plant tissues, plant seeds, and plant cells comprising a heterologous polynucleotide encoding a gene product having tricothecene resistance activity that thereby confers trichothecene resistance to the transgenic plants, plant tissues, plant seeds, and plant cells. Trichothecene resistance activity, as used herein, refers to an activity that reduces or inhibits the phytotoxicity of a trichothecene, particularly to a fungus and/or plant. In a particular embodiment, trichothecene resistance activity refers to an activity that transfers an acetate to the C-3 position of a trichothecene such as T-2 toxin, HT-2 toxin, isotrichodermol, diacetoxyscirpenol (“DAS”), 3-deacetylcalonectrin, 3,15-dideacetylcalonectrin, scirpentriol, neosolaniol; 15-acetyldeoxynivalenol, nivalenol, 4-acetylnivalenol (fusarenone-X), 4,15-diacetylnivalenol, 4,7,15-acetylnivalenol, and deoxynivalenol (“DON”) and their various acetylated derivatives. In another particular embodiment, the gene product having trichthecene resistance activity is a 3-O-acetyltransferase from a trichothecene-producing species of Fusarium, such as Fusarium graminearum or Fusarium sporotrichioides.
摘要翻译：本发明公开了头孢霉烯抗性转基因植物，植物组织，植物种子和植物细胞，其包含编码具有三氯生物抗性活性的基因产物的异源多核苷酸，从而赋予转基因植物，植物组织，植物种子和植物细胞的单端孢霉烯抗性。如本文所用，三氯乙炔抗性活性是指减少或抑制单端孢霉烯特别是真菌和/或植物的植物毒性的活性。在一个具体实施方案中，单端孢霉烯抗性活性是指将乙酸酯转移至单端孢霉烯C-3位的活性，例如T-2毒素，HT-2毒素，异维甲酸，二乙酰氧基蓖麻酚（“DAS”），3-脱乙酰基木聚糖， 3,15-二乙酰胆碱，顺丁三醇，新溶胶甘醇; 15-乙酰基过氧化雪腐镰刀菌烯醇，新戊烯醇，4-乙酰基雪腐镰刀菌烯醇（fisarenone-X），4,15-二乙酰基雪腐镰刀菌烯醇，4,7,15-乙酰基雪腐镰刀菌烯醇和脱氧雪腐镰刀菌烯醇（“DON”）及其各种乙酰化衍生物。在另一个具体实施方案中，具有三辛抗性活性的基因产物是来自产生单端孢霉烯的镰刀菌属物种如禾谷镰刀菌（Fusarium graminearum）或孢子镰刀菌（Fusarium sporotrichioides）的3-O-乙酰转移酶。

4. 发明授权

US06521435B1 Nucleic acid sequences encoding cell wall-degrading enzymes and use to engineer resistance to Fusarium and other pathogens 失效
标题翻译：编码细胞壁降解酶的核酸序列，用于设计对镰刀菌和其他病原体的抗性
公开(公告)号：US06521435B1
公开(公告)日：2003-02-18
申请号：US09649747
申请日：2000-08-28
申请人： Patricia A. Okubara , Ann E. Blechl , Thomas M. Hohn , Randy M. Berka
发明人： Patricia A. Okubara , Ann E. Blechl , Thomas M. Hohn , Randy M. Berka
IPC分类号： C12N900
CPC分类号： C12Y302/01014 , C12N9/2405 , C12N9/2442 , C12N15/8216 , C12N15/8222 , C12N15/8231 , C12N15/8234 , C12N15/8282 , C12Y302/01052
摘要： The invention is directed to nucleic acid sequences derived from Fusarium fungal genes which encode the cell wall-degrading enzymes glucanase, endochitinase, and exochitinase; isolated polypeptides having glucanase, endochitinase or exochitinase activity; recombinant nucleic acid molecules, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides, including expression in plant cells to confer or enhance a plant's resistance to Fusarium and other pathogens.
摘要翻译：本发明涉及来自编码细胞壁降解酶葡聚糖酶，内切壳酶和外切蛋白酶的镰刀霉真菌基因的核酸序列; 分离的多肽具有葡聚糖酶，内切壳酶或外切蛋白酶活性; 包含核酸序列的重组核酸分子，载体和宿主细胞以及用于产生和使用多肽的方法，包括在植物细胞中表达以赋予或增强植物对镰孢属和其他病原体的抗性。

5. 发明授权

US06372479B1 Fusarium sporotrichioides strains for production of B-carotene 失效
标题翻译：用于生产β-胡萝卜素的Fusarium sporotrichioides菌株
公开(公告)号：US06372479B1
公开(公告)日：2002-04-16
申请号：US09656062
申请日：2000-09-06
申请人： James D. Jones , Thomas M. Hohn , Timothy D. Leathers
发明人： James D. Jones , Thomas M. Hohn , Timothy D. Leathers
IPC分类号： C12N115
CPC分类号： C12Y205/01029 , C12N15/52 , C12N15/66
摘要： The instant invention is drawn towards transformed strains of Fusarium sporotrichioides effective for the production of B-carotene. The transformed strains comprise an expression cassette having four genes encoding, respectively, geranylgeranyl-pyrophosphate synthase, phytoene synthase, phytoene desaturase and lycopene cyclase (i.e. Tri5crtE, Tri5crtB, TriScrtI and CrtY). The transformed strains of Fusarium sporotrichioides of the instant invention produce B-carotene at levels of up to 3-4 milligrams per gram of fungus (dry weight).
摘要翻译：本发明涉及有效用于生产β-胡萝卜素的转化株Fusarium sporotrichioides。转化的菌株包含具有分别编码香叶基香叶基 - 焦磷酸合成酶，八氢番茄红素合成酶，八氢番茄红素去饱和酶和番茄红素环化酶（即Tri5crtE，Tri5crtB，TriScrtI和CrtY）的四个基因的表达盒。本发明的转化的镰刀菌孢子孢子菌菌株以每克真菌（干重）高达3-4毫克的量产生β-胡萝卜素。

6. 发明授权

US06184000B2 System for the sequential, directional cloning of multiple DNA sequences 失效
标题翻译：用于顺序定向克隆多个DNA序列的系统
公开(公告)号：US06184000B2
公开(公告)日：2001-02-06
申请号：US09360083
申请日：1999-07-23
申请人： James D. Jones , Thomas M. Hohn , Timothy D. Leathers
发明人： James D. Jones , Thomas M. Hohn , Timothy D. Leathers
IPC分类号： C12N1566
CPC分类号： C12Y205/01029 , C12N15/52 , C12N15/66
摘要： A method which combines the use of polymerase chain reaction (PCR) or oligonucleotide linkers and restriction enzymes which cleave degenerate or variable recognition site sequences to allow the cloning of multiple DNA sequences into a vector is disclosed. In this invention, a plurality of unrelated DNA sequences may be directionally cloned within a single vector by adding onto the ends of the sequences, restriction sites with specific sequences which are cleaved by corresponding restriction endonucleases which recognize degenerate or variable recognition sites and which generate cohesive ends upon cleavage. The compatibility (or ability to anneal) of the cohesive ends on different DNA sequences is controlled by the choice of the nucleotide sequence within the recognition sequences of the restriction endonucleases, allowing the DNA sequences to be inserted or joined in any desired orientation. These restriction sites may be selectively incorporated onto either or both end of any DNA sequence of interest using oligonucleotidelinkers or using PCR by adding the restriction sites onto the termini of the 5′ and/or 3′ primers.
摘要翻译：公开了一种结合使用聚合酶链反应（PCR）或寡核苷酸接头和限制酶，其切割简并或可变识别位点序列以允许将多个DNA序列克隆到载体中的方法。在本发明中，多个不相关的DNA序列可以通过在序列的末端加入具有特定序列的限制性位点，通过相应的限制性内切核酸酶切割而识别简并或可变识别位点并产生粘性结束于分裂。通过选择限制性内切核酸酶的识别序列内的核苷酸序列来控制不同DNA序列上的粘性末端的相容性（或退火能力），允许以任何期望的方向插入或连接DNA序列。这些限制性位点可以使用寡核苷酸维持索引选择性地并入目的DNA序列的任一端或两端，或者通过将限制性位点添加到5'和/或3'引物的末端上来进行PCR。

7. 发明授权

US06696282B2 Fusarium sporotrichioides strains for production of lycopene 失效
标题翻译：用于生产番茄红素的镰刀菌孢子菌素菌株
公开(公告)号：US06696282B2
公开(公告)日：2004-02-24
申请号：US10046849
申请日：2002-01-14
申请人： James D. Jones , Thomas M. Hohn , Timothy D. Leathers
发明人： James D. Jones , Thomas M. Hohn , Timothy D. Leathers
IPC分类号： C12N115
CPC分类号： C12Y205/01029 , C12N15/52 , C12N15/66
摘要： The instant invention is drawn towards transformed strains of Fusarium sporotrichioides effective for the production of lycopene. The transformed strains comprise an expression cassette having three genes encoding, respectively, geranylgeranyl-pyrophosphate synthase, phytoene synthase and phytoene desaturase (i.e. Tri5crtE, Tri5crtB and Tri5crtl). The transformed strains of Fusarium sporotrichioides of the instant invention produce lycopene at levels of up to 0.5 milligrams per gram culture dry weight.
摘要翻译：本发明涉及有效用于生产番茄红素的转化菌Fosarium sporotrichioides。转化的菌株包含具有分别编码香叶基香叶基 - 焦磷酸合成酶，八氢番茄红素合成酶和八氢番茄红素去饱和酶（即Tri5crtE，Tri5crtB和Tri5crt1）的三个基因的表达盒。本发明的转化的尖孢镰孢菌孢子菌素产生番茄红素，其水平高达每毫升培养干重0.5毫克。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式