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1. 发明申请

US20070054358A1 Insertion sequence-free bacteria 有权
标题翻译：插入无序细菌
公开(公告)号：US20070054358A1
公开(公告)日：2007-03-08
申请号：US11400711
申请日：2006-04-07
申请人： Frederick Blattner , John Campbell , David Frisch , Guy Plunkett , Gyorgy Posfai
发明人： Frederick Blattner , John Campbell , David Frisch , Guy Plunkett , Gyorgy Posfai
IPC分类号： C12P21/06 , C07K14/195 , C12N1/21 , C12P19/34 , C07H21/04 , C12N15/74
CPC分类号： C12N1/20 , C07K14/245 , C12N1/08 , C12N15/70 , C12P19/34 , C12P21/02 , C12R1/19
摘要： A bacteria lacking genomic and non-genomic IS elements is provided. The bacteria may be more stable and useful for the production of amino acids, polypeptides, nucleic acids and other products.
摘要翻译：提供了缺乏基因组和非基因组IS元件的细菌。细菌可能更稳定并且可用于生产氨基酸，多肽，核酸和其它产物。

2. 发明授权

US08119365B2 Insertion sequence-free bacteria 有权
标题翻译：插入无序细菌
公开(公告)号：US08119365B2
公开(公告)日：2012-02-21
申请号：US11400711
申请日：2006-04-07
申请人： Frederick R. Blattner , John W. Campbell , David Frisch , Guy Plunkett , Gyorgy Posfai
发明人： Frederick R. Blattner , John W. Campbell , David Frisch , Guy Plunkett , Gyorgy Posfai
IPC分类号： C12N15/09 , C12N15/10 , C12N1/20
CPC分类号： C12N1/20 , C07K14/245 , C12N1/08 , C12N15/70 , C12P19/34 , C12P21/02 , C12R1/19
摘要： A bacteria lacking genomic and non-genomic IS elements is provided. The bacteria may be more stable and useful for the production of amino acids, polypeptides, nucleic acids and other products.
摘要翻译：提供了缺乏基因组和非基因组IS元件的细菌。细菌可能更稳定并且可用于生产氨基酸，多肽，核酸和其它产物。

3. 发明申请

US20120219994A1 PROPHAGE ELEMENT-FREE BACTERIA 有权
标题翻译：推荐元素免费细菌
公开(公告)号：US20120219994A1
公开(公告)日：2012-08-30
申请号：US13363848
申请日：2012-02-01
申请人： FREDERICK R. BLATTNER , John W. Campbell , David Frisch , Guy Plunkett, III , Gyorgy Posfai
发明人： FREDERICK R. BLATTNER , John W. Campbell , David Frisch , Guy Plunkett, III , Gyorgy Posfai
IPC分类号： C12N1/20 , C12N1/21 , C12P21/02
CPC分类号： C07K14/245 , C12N1/08 , C12N1/20 , C12N15/70 , C12P19/34 , C12P21/02 , C12R1/19
摘要： The present invention provides a bacterium having a genome that is genetically engineered to be smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.
摘要翻译：本发明提供了一种具有遗传工程化以小于其天然亲本菌株基因组的基因组的细菌。具有较小基因组的细菌可以更有效地产生商业产品。本发明还提供从细菌基因组中删除基因和其他DNA序列的方法。该方法提供精确的缺失，很少引入缺失位点周围基因组DNA序列的突变。因此，该方法可用于在细菌中产生一系列缺失，而不增加基因组内不希望的同源重组的可能性。此外，本发明提供的一些方法也可用于用期望的DNA序列替代细菌基因组的区域。

4. 发明授权

US08765408B2 Prophage element-free bacteria 有权
标题翻译：无菌元素细菌
公开(公告)号：US08765408B2
公开(公告)日：2014-07-01
申请号：US13363848
申请日：2012-02-01
申请人： Frederick R. Blattner , John W. Campbell , David Frisch , Guy Plunkett, III , Gyorgy Posfai
发明人： Frederick R. Blattner , John W. Campbell , David Frisch , Guy Plunkett, III , Gyorgy Posfai
IPC分类号： C12P21/06 , C12N1/20 , C12N1/21
CPC分类号： C07K14/245 , C12N1/08 , C12N1/20 , C12N15/70 , C12P19/34 , C12P21/02 , C12R1/19
摘要： The present invention provides a bacterium having a genome that is genetically engineered to be smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.
摘要翻译：本发明提供了一种具有遗传工程化以小于其天然亲本菌株的基因组的基因组的细菌。具有较小基因组的细菌可以更有效地产生商业产品。本发明还提供从细菌基因组中删除基因和其他DNA序列的方法。该方法提供精确的缺失，很少引入缺失位点周围基因组DNA序列的突变。因此，该方法可用于在细菌中产生一系列缺失，而不增加基因组内不希望的同源重组的可能性。此外，本发明提供的一些方法也可用于用期望的DNA序列替代细菌基因组的区域。

5. 发明申请

US20110053274A1 LAC EXPRESSION SYSTEM 审中-公开
标题翻译： LAC表达系统
公开(公告)号：US20110053274A1
公开(公告)日：2011-03-03
申请号：US12745443
申请日：2008-11-26
申请人： Frederick Blattner , Dimitry Schevchenko , David Frisch , John Campbell
发明人： Frederick Blattner , Dimitry Schevchenko , David Frisch , John Campbell
IPC分类号： C12N15/87 , C07H21/04 , C12N1/21
CPC分类号： C12N15/72
摘要： Provided herein is a nucleic acid comprising a mutant lac operator operably linked to a gene of interest, a host cell comprising the nucleic acid, and a method of using the host cell to express the gene of interest. Also provided is a recA-mediated cloning method.
摘要翻译：本文提供的是包含可操作地连接到目标基因的突变型lac操纵子的核酸，包含该核酸的宿主细胞，以及使用该宿主细胞表达感兴趣的基因的方法。还提供了recA介导的克隆方法。

6. 发明申请

US20100304445A1 NUCLEIC ACID PACKAGING SYSTEM 审中-公开
标题翻译：核酸包装系统
公开(公告)号：US20100304445A1
公开(公告)日：2010-12-02
申请号：US12664899
申请日：2008-06-16
申请人： Waclaw Szybalski , Frederick Blattner , David Frisch , Douglas Wieczorek
发明人： Waclaw Szybalski , Frederick Blattner , David Frisch , Douglas Wieczorek
IPC分类号： C12N15/64 , C12P19/34
CPC分类号： C07H1/00 , C07H5/10 , C12Q1/686 , C12Q1/6869 , C12Q2565/537 , C12Q2565/515 , C12Q2535/125
摘要： The present invention relates to cloning target nucleic acids using phage packaging mechanisms. Packaging initiation sites may be introduced into the target DNA. Components of a phage packaging system may be combined with the target DNA to package the DNA into phage capsids. The packaged DNA may be used to create a library of target nucleic acids, or it may be sequenced.
摘要翻译：本发明涉及使用噬菌体包装机制克隆靶核酸。可以将包装起始位点引入目标DNA。噬菌体包装系统的组分可以与目标DNA组合以将DNA包装到噬菌体衣壳中。包装的DNA可用于产生靶核酸文库，或者可以测序。

7. 发明申请

US20070148674A1 OPTICAL FINGERPRINTING OF NUCLEIC ACID SEQUENCES 审中-公开
标题翻译：核酸序列的光学指纹
公开(公告)号：US20070148674A1
公开(公告)日：2007-06-28
申请号：US11615695
申请日：2006-12-22
申请人： Mark BERRES , David Frisch , Fredrick Blattner
发明人： Mark BERRES , David Frisch , Fredrick Blattner
IPC分类号： C12Q1/68 , C12M3/00
CPC分类号： G01N33/5308 , C12Q1/6816 , C12Q1/6837 , C12Q1/6869 , C12Q2565/601 , C12Q2565/513 , C12Q2522/101
摘要： The present invention provides methods and apparatus for the optical fingerprinting of nucleic acid molecules. In certain embodiments, the invention provides methods for labeling nucleic acid molecules using-site-specific nucleic acid binding partners that bind to nucleic acids without cleaving the molecule. The nucleic acid binding partners can be labeled directly with a fluorophore, such as a quantum dot (QD), or indirectly. Examples of suitable binding partners include cut-deficient restriction endonucleases (cdREs), transcription factors, the binding domains of nucleic acid polymerases, antibodies and the like. The methods disclosed make the assembly of fingerprint contigs easier and allows for digitizing the results so as to provide for easier computer manipulation and assembly. The invention also includes a microarray, the microarray designed to provide for easy deposition and high-throughput fingerprinting. The invention also provides for methods of using the microarrays. The invention also provides kits for the use of the invention.
摘要翻译：本发明提供了核酸分子的光学指纹图谱的方法和装置。在某些实施方案中，本发明提供了使用与核酸结合但不裂解该分子的位点特异性核酸结合配偶体标记核酸分子的方法。核酸结合配偶体可直接用荧光团（例如量子点（QD））或间接地标记。合适的结合配偶体的实例包括切断缺陷型限制性内切核酸酶（cdRE），转录因子，核酸聚合酶的结合结构域，抗体等。所公开的方法使得指纹重叠群的组装更容易，并允许数字化结果，从而提供更容易的计算机操纵和组装。本发明还包括微阵列，微阵列被设计成提供易于沉积和高通量指纹。本发明还提供了使用微阵列的方法。本发明还提供了用于本发明的试剂盒。

8. 外观设计

USD668067S1 Side-lit mirror 有权
标题翻译：侧光镜
公开(公告)号：USD668067S1
公开(公告)日：2012-10-02
申请号：US29391355
申请日：2011-05-06
申请人： David Frisch
设计人： David Frisch

9. 发明申请

US20100144548A1 VECTOR SYSTEMS 审中-公开
标题翻译：矢量系统
公开(公告)号：US20100144548A1
公开(公告)日：2010-06-10
申请号：US12375911
申请日：2007-08-03
申请人： Waclaw Szybalski , Jadwiga Wild , Zdenka Hradecna , David Frisch , Frederick R. Blattner , Katarzyna Gromek
发明人： Waclaw Szybalski , Jadwiga Wild , Zdenka Hradecna , David Frisch , Frederick R. Blattner , Katarzyna Gromek
IPC分类号： C40B40/02 , C12N15/74 , C12N1/21 , C12N15/64 , C40B50/06 , C12N5/071
CPC分类号： C12N15/70 , C12N15/1093 , C12N15/64 , C12N15/66
摘要： The present invention relates generally to the field of molecular biology and genomics. More specifically, the present invention concerns the cloning of nucleic acid molecules and the production of nucleic acid libraries, as well as the expression of recombinant proteins and bactofection.
摘要翻译：本发明一般涉及分子生物学和基因组学领域。更具体地，本发明涉及核酸分子的克隆和核酸文库的产生，以及重组蛋白质和细菌转染的表达。

10. 发明申请

US20130027913A1 MAKEUP MIRROR WITH LIGHT SOURCE 审中-公开
标题翻译：用光源制造镜子
公开(公告)号：US20130027913A1
公开(公告)日：2013-01-31
申请号：US13194416
申请日：2011-07-29
申请人： David Frisch , Kelly O'Brien Martelli , Kelly Shea Kuethe
发明人： David Frisch , Kelly O'Brien Martelli , Kelly Shea Kuethe
IPC分类号： F21V33/00
CPC分类号： A45D42/10
摘要： A makeup mirror having a light source configured to have output that not only enhances the visibility of objects as they are reflected by the mirror, but also optimizes visual acuity of the reflected image as captured by the human eye. In some cases, the makeup mirror allows for separate adjustment of the mirror and the light source, which, in combination with the light source, enables the reflected objects to be lit in uniform manner and for optimized human visual acuity while allowing for areas of the objects to be examined in detail as desired.
摘要翻译：具有光源的化妆镜被配置为具有输出，其不仅增强了被反射镜反射的物体的可视性，而且还优化了由人眼捕获的反射图像的视敏度。在某些情况下，化妆镜允许对镜子和光源进行单独的调整，光源与光源组合使得能够以均匀的方式点亮反射物体并且优化人的视力，同时允许根据需要详细检查的对象。

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