专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06537783B1 Prefunctionalized nucleotide and process for amplifying a sequence using a prefunctionalized nucleotide 失效
标题翻译：预功能化核苷酸和使用预功能化核苷酸扩增序列的方法
公开(公告)号：US06537783B1
公开(公告)日：2003-03-25
申请号：US09214658
申请日：1999-01-11
申请人： Françoise Guillou-Bonnici , Eric Defranc , Antoine Hoang , Ali Laayoun , Jean Lhomme , Emmanuelle Trevisiol
发明人： Françoise Guillou-Bonnici , Eric Defranc , Antoine Hoang , Ali Laayoun , Jean Lhomme , Emmanuelle Trevisiol
IPC分类号： C12P1934
CPC分类号： C12P19/34 , C07H19/06 , C07H21/00 , C12N15/10 , C12P19/30 , C12Q1/686 , C12Q2527/125 , C12Q2525/101
摘要： A target nucleic acid sequence amplification method wherein at least the target nucleic acid sequence, at least one oligonucleotide primer specific for the target sequence, one or more enzymatic activities and nucleotides are provided, and the target sequence is amplified under conditions suitable in particular for said enzymatic or activity or activities, is disclosed. According to the method, at least one of the nucleotides is a prefunctionalized nucleotide that differs from the other nucleotides at least in that it includes at least one unprotected reactive covalence function in at least one predetermined site of the base of said nucleotide, in order to give a prefunctionalized amplificati product including at least one such prefunctionalized nucleotide. Nucleotides for carrying out the method are also disclosed.
摘要翻译：一种靶核酸序列扩增方法，其中至少提供所述目标核酸序列，至少一种对靶序列特异性的寡核苷酸引物，一种或多种酶活性和核苷酸，并且所述靶序列在适合于所述酶或活性或活性。根据该方法，至少一个核苷酸是与其它核苷酸不同的前功能化核苷酸，至少在至少一个所述核苷酸的碱基的一个预定位点中包含至少一个未保护的反应性共价功能，以便得到包括至少一种这样的前官能化核苷酸的前功能化扩增产物。还公开了用于实施该方法的核苷酸。

2. 发明授权

US06875858B1 Functionalized polynucleotide compound, optionally marked and method for detecting a target nucleic acid 有权
标题翻译：官能化多核苷酸化合物，任选地标记和检测靶核酸的方法
公开(公告)号：US06875858B1
公开(公告)日：2005-04-05
申请号：US09869796
申请日：2000-01-05
申请人： Eric DeFrancq , Ali Laayoun , Jean Lhomme , Emmanuelle Trevisiol
发明人： Eric DeFrancq , Ali Laayoun , Jean Lhomme , Emmanuelle Trevisiol
IPC分类号： C07H21/00 , C12P19/34 , C12Q1/68 , C07H19/04
CPC分类号： C07H21/00 , C12P19/34 , C12Q1/68
摘要： The invention relates to a functionalized compound of general formula (I): in which W represents a nucleotide analog, L represents a linker arm comprising at least four atoms, R1 represents a linear or branched alkyl chain, A functionalized polynucleotide comprising at least one said compound and a labeled functionalized polynucleotide comprising at least one functionalized compound corresponding to the formula (I′).A method for detecting a target nucleic acid using a said compound is described.
摘要翻译：本发明涉及通式（I）的官能化化合物：其中W表示核苷酸类似物，L表示包含至少四个原子的连接基团，R 1表示直链或支链烷基链，A官能化多核苷酸包含至少一种所述化合物和包含至少一种对应于式（I'）的官能化化合物的标记的官能化多核苷酸，描述了使用所述化合物检测靶核酸的方法。

3. 发明授权

US07060441B2 Method for fragmenting and labeling DNA involving abasic sites and phosphate labeling 失效
标题翻译：用于分离和标记涉及无碱基位点和磷酸盐标记的DNA的方法
公开(公告)号：US07060441B2
公开(公告)日：2006-06-13
申请号：US10137460
申请日：2002-05-03
申请人： Cecile Bourget , Mitsuharu Kotera , Jean Lhomme , Emmanuelle Trevisiol , Ali Laayoun , Christelle Tora , Isabelle Sothier
发明人： Cecile Bourget , Mitsuharu Kotera , Jean Lhomme , Emmanuelle Trevisiol , Ali Laayoun , Christelle Tora , Isabelle Sothier
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6806 , C12Q1/6837 , C12Q2600/156 , Y10T436/143333 , C12Q2525/119 , C12Q2525/101 , C12Q2523/107
摘要： A single- or double-stranded deoxyribonucleic acid (DNA) is fragmented and labeled by chemically fragmenting the DNA by creating at least one abasic site on the DNA, and attaching a marker to at least one of the fragments by means of a labeling reagent, the reagent covalently and predominantly coupling to at least one phosphate of the fragment. This method can be applied in the field of diagnosis.
摘要翻译：单链或双链脱氧核糖核酸（DNA）被片段化，并通过在DNA上产生至少一个脱碱基位点并通过标记试剂将标记连接至至少一个片段，该试剂共价并主要与该片段的至少一个磷酸酯偶联。该方法可应用于诊断领域。

4. 发明授权

US07338805B2 Labeling reagents, methods for synthesizing such reagents and methods for detecting biological molecules 有权
标题翻译：标记试剂，合成这些试剂的方法和检测生物分子的方法
公开(公告)号：US07338805B2
公开(公告)日：2008-03-04
申请号：US10137454
申请日：2002-05-03
申请人： Cecile Bourget , Jean Lhomme , Ali Laayoun , Mitsuharu Kotera , Emmanuelle Trevisiol , Lionel Menou , Eloy Bernal Mendez
发明人： Cecile Bourget , Jean Lhomme , Ali Laayoun , Mitsuharu Kotera , Emmanuelle Trevisiol , Lionel Menou , Eloy Bernal Mendez
IPC分类号： G01N37/00 , G01N33/48
CPC分类号： C07D209/08 , C07C245/14 , C07C245/18 , C07D311/90 , C07D495/04 , Y10T436/13 , Y10T436/143333
摘要： The present invention relates to a temperature-stable labeling reagent of formula: in which R1 represents H or an alkyl, aryl or substituted aryl group, R2 represents a detectable marker or at least two detectable markers linked together by at least one multimeric structure, L is a linking arm containing a linear succession of at least two covalent bonds and n an integer equal to 0 or 1, R3 and R4 represent independently of each other: H, NO2, Cl, Br, F, I, R2-(L)n-Y—X—, OR, SR, NR2, R, NHCOR, CONHR, COOR with R=alkyl or aryl, A is a linking arm containing at least one covalent double bond allowing conjugation of the diazo functional group with the aromatic ring and u is an integer between 0 and 2, preferably equal to 0 or 1, and —Y—X— represents —CONH—, —NHCO—, —CH2O—, —CH2S—. The present invention also describes a method for synthesizing said markers as well as applications for labeling biological molecules, in particular nucleic acids, with a labeling reagent carrying the diazomethyl functional group.The invention finds a preferred application in the field of diagnosis.
摘要翻译：本发明涉及下式的温度稳定的标记试剂：其中R 1表示H或烷基，芳基或取代的芳基，R 2表示可检测标记或至少两个通过至少一个多聚体结构连接在一起的可检测标记，L是含有至少两个共价键的线性连续并且n为等于0或1的整数的连接臂，R 3和 R 4彼此独立地表示：H，NO 2，Cl，Br，F，I，R 2 - （L）_{O-，-CH 2 S-。本发明还描述了一种用于合成所述标记的方法以及用携带重氮甲基官能团的标记试剂标记生物分子，特别是核酸的应用。本发明在诊断领域找到优选的应用。}

5. 发明申请

US20100136538A1 MARKING REAGENTS BEARING DIAZO AND NITRO FUNCTIONS, METHODS FOR THE SYNTHESIS OF SUCH REAGENTS AND METHODS FOR DETECTING BIOLOGICAL MOLECULES 失效
标题翻译：携带DIAZO和NITRO功能的标记试剂，合成这些试剂的方法和检测生物分子的方法
公开(公告)号：US20100136538A1
公开(公告)日：2010-06-03
申请号：US12451502
申请日：2008-06-09
申请人： Alain Laurent , Ali Laayoun , Mitsuharu Kotera
发明人： Alain Laurent , Ali Laayoun , Mitsuharu Kotera
IPC分类号： C12Q1/68 , C07D495/04 , C07H21/00 , C12P19/34
CPC分类号： C07D495/04 , Y10T436/13
摘要： The present invention relates to a labeling reagent of formula: in which: R1 represents at least one detectable label, L and A are each a linker arm, n is an integer equal to 1, and u is an integer between 0 and 2. The present invention also describes a method of synthesizing said markers and also applications for the labeling of biological molecules, more particularly nucleic acids, with a labeling reagent bearing diazo and nitro functions. The invention is particularly suitable for use in the diagnostics field.
摘要翻译：本发明涉及下式的标记试剂：其中：R1表示至少一个可检测标记，L和A各自为连接臂，n为等于1的整数，u为0至2之间的整数。本发明还描述了一种合成所述标记的方法，以及用于用具有重氮和硝基功能的标记试剂标记生物分子，更特别是核酸的应用。本发明特别适合用于诊断领域。

6. 发明申请

US20130253179A1 Functionalization Processes and Reactants Used in Such Processes Using an Isatoic Anhydride or a Derivative Thereof, Biological Molecules Thus Treated and Kits 审中-公开
标题翻译：官能化方法和反应物在这些方法中使用的盐酸酐或其衍生物，如此处理的生物分子和试剂盒
公开(公告)号：US20130253179A1
公开(公告)日：2013-09-26
申请号：US13992049
申请日：2011-12-05
申请人： Arnaud Burr , Ali Laayoun , Alain Laurent
发明人： Arnaud Burr , Ali Laayoun , Alain Laurent
IPC分类号： C07H21/02
CPC分类号： C07H21/02 , C07D413/12 , C07D413/14 , C07D495/04 , C12N15/111 , C12N2330/30 , C12Q1/68 , C12Q2563/179
摘要： The present invention relates to a process of functionalising at least one ribonucleic acid (RNA) molecule which comprises the following steps: a) having at least: a binding molecule constituted by an isatoic anhydride or a derivative thereof, a group of interest, and a binding arm linking the binding molecule with the group of interest, b) reacting the anhydride function of the binding molecule with at least one hydroxyl group in: position 2′ of the ribose of one of the RNA nucleotides, and/or position(s) 2′ and/or 3′ of the ribose of the nucleotide at the 3′ terminal end of the RNA, and c) obtaining an anthranilate linking, via the binding arm, the RNA to the group of interest. The invention also relates to a functionalising reagent able to be used in such processes, a functionalised biological RNA molecule capable of being obtained by these processes and a kit for detecting a target RNA molecule comprising such a reagent.Said invention finds a preferential application in the field of in vitro diagnosis.
摘要翻译：本发明涉及功能化至少一种核糖核酸（RNA）分子的方法，该方法包括以下步骤：a）至少具有：由靛红酸酐或其衍生物，感兴趣的组和将结合分子与感兴趣的基团连接的结合臂，b）使结合分子的酸酐官能团与至少一个羟基在一个RNA核苷酸的核糖的位置2'和/ 2'和/或3'，并且c）获得通过结合臂将RNA连接到感兴趣的组中的邻氨基苯甲酸酯。本发明还涉及能够用于这些方法的功能化试剂，能够通过这些方法获得的官能化生物RNA分子和用于检测包含这种试剂的靶RNA分子的试剂盒。所述发明在体外诊断领域中优先应用。

7. 发明授权

US07691635B2 Labeling reagents, methods for the synthesis of such reagents and methods for the detection of biological molecules 失效
标题翻译：标记试剂，合成这些试剂的方法和检测生物分子的方法
公开(公告)号：US07691635B2
公开(公告)日：2010-04-06
申请号：US10590973
申请日：2005-03-24
申请人： Ali Laayoun , Eloy Bernal-Mendez
发明人： Ali Laayoun , Eloy Bernal-Mendez
IPC分类号： G01N37/00 , G01N33/48 , C07D239/70 , A61K31/4188
CPC分类号： C07D495/04 , Y10T436/13 , Y10T436/143333
摘要： The present invention relates to a temperature-stable labeling reagent of formula (0): in which: R1 represents H or an alkyl, aryl or substituted aryl group, R2 represents a detectable marker or at least two detectable markers interlinked by at least one multimeric structure, L is a linker arm comprising a linear chain of at least two covalent bonds and n is an integer equal to 0 or 1, R3 and R4 represent, independently of one another: H, NO2, Cl, Br, F, I, R2-(L)n-Y—X—, OR, SR, NR2, R, NHCOR, CONHR, COOR, —CO—NH—(CH2)3—(O—CH2—CH2)3—CH2—NH—R2, —CO—NH—(CH2)3—(O—CH2—CH2)4—CH2—NH—R2 with R=alkyl or aryl, A is a linker arm comprising at least one covalent double bond enabling the conjugation of the diazo function with the aromatic ring and u is an integer between 0 and 2, preferably 0 or 1, —Y—X— represents —CONH—, —NHCO—, —CH2O—, —CH2S—, —Z— represents —NH—, —NHCO—, —CONH— or —O—, m is an integer between 1 and 10, preferably between 1 and 3, and p is an integer between 1 and 10, preferably between 1 and 3. The present invention also describes a method for the synthesis of said labels and also applications for the labeling of biological molecules, in particular of nucleic acids, with a labeling reagent bearing the diazomethyl function.The invention is particularly suitable for use in the field of diagnostics.
摘要翻译：本发明涉及式（O）的温度稳定的标记试剂：其中：R 1表示H或烷基，芳基或取代的芳基，R 2表示可检测标记或至少两个可检测标记，其与至少一个多聚体结构，L是包含至少两个共价键的直链的连接臂，n是等于0或1的整数，R 3和R 4彼此独立地表示：H，NO 2，Cl，Br，F，I， R 2 - （L）n Y-X，OR，SR，NR 2，R，NHCOR，CONHR，COOR，-CO-NH-（CH 2）3 - （O-CH 2 -CH 2）3 -CH 2 -NH-R 2， CO-NH-（CH 2）3 - （O-CH 2 -CH 2）4 -CH 2 -NH-R 2，其中R =烷基或芳基，A是连接臂，其包含至少一个共价双键，使得重氮官能团与芳环和u是0和2之间的整数，优选0或1，-Y-X-表示-CONH - ， - NHCO - ， - CH 2 O - ， - CH 2 S - ， - Z-表示-NH-，-NHCO - ， - CONH-或-O-，m是1 a之间的整数 nd 10，优选在1和3之间，p是1至10之间，优选1至3之间的整数。本发明还描述了合成所述标记的方法以及用于标记生物分子的应用，特别是的核酸，具有带有重氮甲基功能的标记试剂。本发明特别适用于诊断领域。

8. 发明授权

US06376179B1 Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby 有权
标题翻译：用于标记核糖核酸的方法和由此获得的标记的RNA片段
公开(公告)号：US06376179B1
公开(公告)日：2002-04-23
申请号：US09446156
申请日：1999-12-17
申请人： Ali Laayoun
发明人： Ali Laayoun
IPC分类号： C12Q168
CPC分类号： C07H21/00
摘要： The present invention relates to a process for labeling a synthetic or natural ribonucleic acid (RNA). It also relates to RNA fragments, which have been labeled by fragmenting the RNA to free a terminal phosphate of each fragment for further reaction, and labeling each fragment at the freed terminal phosphate which is located at the 3′ end and/or the 5′ end of each fragment of the RNA, and to the use of such RNA fragments, for example, in the field of medical diagnosis.
摘要翻译：本发明涉及用于标记合成或天然核糖核酸（RNA）的方法。它还涉及RNA片段，其已经通过片段化RNA来标记以释放每个片段的末端磷酸酯用于进一步的反应，并且在位于3'末端和/或5'端的释放的磷酸末端标记每个片段， RNA的每个片段的末端，以及例如在医学诊断领域中使用这样的RNA片段。

9. 发明授权

US09365894B2 Methods and kits for avoiding amplification of contaminating nucleic acids 有权
标题翻译：用于避免污染性核酸扩增的方法和试剂盒
公开(公告)号：US09365894B2
公开(公告)日：2016-06-14
申请号：US13131174
申请日：2010-01-04
申请人： Ali Laayoun , Alain Troesch
发明人： Ali Laayoun , Alain Troesch
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6848 , C12Q2523/125
摘要： A method of amplifying a nucleic acid of interest comprises (i) treating a biological sample chemically or enzymatically to permit conversion of one type of nucleic acid base to another type of base, (ii) purifying the treated biological sample before adding amplification primers and amplification reagents to the biological sample, (iii) adding the amplification primers and amplification reagents to the biological sample, each primer being constituted of three different types of bases and being specific to a converted nucleic acid of interest or to a nucleic acid that is complementary to the converted nucleic acid of interest, and (iv) amplifying the converted nucleic acid of interest provided that the nucleic acid of interest was present in the biological sample. In the method, amplification of contaminating nucleic acids is avoided by converting the one type of nucleic acid base to another type of base prior to adding the amplification reagents to the biological sample.
摘要翻译：扩增目的核酸的方法包括（i）以化学或酶学方式处理生物样品以允许将一种类型的核酸碱基转化为另一种类型的碱，（ii）在加入扩增引物和扩增前纯化经处理的生物样品生物样品的试剂，（iii）将扩增引物和扩增试剂加入到生物样品中，每个引物由三种不同类型的碱基构成，并且对转化的目的核酸具有特异性，转化的目标核酸，以及（iv）扩增所转化的转化的核酸，只要感兴趣的核酸存在于生物样品中即可。在该方法中，通过在向生物样品中加入扩增试剂之前将一种类型的核酸碱基转化为另一种碱基来避免污染性核酸的扩增。

10. 发明授权

US09266902B2 Labelling reagents having a pyridine nucleus bearing a diazomethyl function, process for synthesis of such reagents and processes for detection of biological molecules 有权
标题翻译：具有具有重氮甲基功能的吡啶核的标记试剂，用于合成这些试剂的方法和检测生物分子的方法
公开(公告)号：US09266902B2
公开(公告)日：2016-02-23
申请号：US13001712
申请日：2009-07-28
申请人： Alain Laurent , Ali Laayoun , Mitsuhara Kotera
发明人： Alain Laurent , Ali Laayoun , Mitsuhara Kotera
IPC分类号： C07D333/24 , C07D233/32 , C07D213/24 , C07D495/04
CPC分类号： C07D495/04 , C07D213/24 , C07D233/32 , C07D333/24 , Y10T436/143333
摘要： A process for synthesis of a labelling reagent, a process for the labelling of a biological molecule, a labelled biological molecule obtained by the process, a process for labelling and fragmentation of a single or double strand nucleic acid, a labelled nucleic acid capable of being obtained by the process, a kit for detection of a target nucleic acid containing a labelled nucleic acid, a solid support onto which is attached a reagent and a process for capture of nucleic acids.
摘要翻译：标记试剂的合成方法，生物分子的标记方法，通过该方法得到的标记的生物分子，单链或双链核酸的标记和断裂的方法，能够被通过该方法获得用于检测含有标记核酸的靶核酸的试剂盒，其上附着有试剂的固体支持物和捕获核酸的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式