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1. 发明申请

US20080233632A1 Process for Labeling and Purification of Nucleic Acids of Interest Present in a Biological Sample to be Treated in a Single Reaction Vessel 审中-公开
标题翻译：用于在单个反应容器中处理的生物样品中存在的感兴趣的核酸的标记和纯化方法
公开(公告)号：US20080233632A1
公开(公告)日：2008-09-25
申请号：US11658028
申请日：2005-07-21
申请人： Ali Laayoun , Lionel Menou , Frederic Ginot
发明人： Ali Laayoun , Lionel Menou , Frederic Ginot
IPC分类号： C07H1/00
CPC分类号： C07D333/00 , C07D233/32 , C07D495/04 , C07H1/08 , C07H21/00
摘要： The present invention relates to a process for labeling and purification of nucleic acids of interest present in a biological sample to be treated, comprising: taking a single reaction vessel, introducing into the reaction vessel: the biological sample, at least one labeling reagent for nucleic acids, at least one solid support enabling the adsorption of said nucleic acids, any ingredient necessary for the labeling of the nucleic acids and/or for the immobilization of said nucleic acids on the support, incubating the contents of the reaction vessel, and isolating the nucleic acids thus labeled. The invention finds a preferred application in the diagnostics field.
摘要翻译：本发明涉及一种用于标记和纯化待处理的生物样品中感兴趣的核酸的方法，包括：取单个反应容器，将反应容器引入生物样品，至少一种用于核酸的标记试剂酸，至少一种能够吸附所述核酸的固体支持物，用于标记核酸所必需的任何成分和/或将所述核酸固定在载体上，孵育反应容器的内容物，并分离如此标记的核酸。本发明在诊断领域中找到了优选的应用。

2. 发明授权

US08637322B2 Method for labeling or treating a biological sample containing biological molecules of interest, in particular nucleic acids 有权
标题翻译：用于标记或处理含有感兴趣的生物分子，特别是核酸的生物样品的方法
公开(公告)号：US08637322B2
公开(公告)日：2014-01-28
申请号：US11919668
申请日：2006-05-31
申请人： Eloy Bernal-Mendez , Ali Laayoun , Lionel Menou
发明人： Eloy Bernal-Mendez , Ali Laayoun , Lionel Menou
IPC分类号： G01N33/48 , C07H1/00
CPC分类号： G01N33/58 , G01N33/532 , G01N33/54353 , Y10T436/11 , Y10T436/143333
摘要： The present invention relates to a method for labeling biological molecules of interest contained in a biological sample, consisting in: a) providing a reaction vessel, b) immobilizing capture molecules, which are capable of binding a label of the biological molecules of interest, on a solid support of the vessel, c) introducing the biological sample but also at least one label of the biological molecules of interest into said vessel and optionally any ingredient required for labeling or prelabeling the molecules of interest, d) incubating the vessel content and immobilizing the label which is not reacted with the molecules of interest by binding to the capture molecules, and e) using the labeled molecules of interest for subsequent steps. A method for treating a biological sample is also disclosed.Said invention is preferably used in a manual or automated method for purifying nucleic acids.
摘要翻译：本发明涉及用于标记生物样品中所含感兴趣生物分子的方法，其包括：a）提供反应容器，b）使能够将感兴趣的生物分子的标记结合的捕获分子固定在所述容器的固体支持物，c）将所述生物学样品引入所述生物样品，还将所述生物分子的至少一个标记物引入所述容器，以及任选的任何标记或预标记目的分子所需的成分，d）孵育所述容器内容物并固定通过结合捕获分子不与感兴趣的分子反应的标记，和e）使用目的标记的分子进行后续步骤。还公开了一种用于处理生物样品的方法。所述发明优选以用于纯化核酸的手动或自动化方法使用。

3. 发明授权

US07338805B2 Labeling reagents, methods for synthesizing such reagents and methods for detecting biological molecules 有权
标题翻译：标记试剂，合成这些试剂的方法和检测生物分子的方法
公开(公告)号：US07338805B2
公开(公告)日：2008-03-04
申请号：US10137454
申请日：2002-05-03
申请人： Cecile Bourget , Jean Lhomme , Ali Laayoun , Mitsuharu Kotera , Emmanuelle Trevisiol , Lionel Menou , Eloy Bernal Mendez
发明人： Cecile Bourget , Jean Lhomme , Ali Laayoun , Mitsuharu Kotera , Emmanuelle Trevisiol , Lionel Menou , Eloy Bernal Mendez
IPC分类号： G01N37/00 , G01N33/48
CPC分类号： C07D209/08 , C07C245/14 , C07C245/18 , C07D311/90 , C07D495/04 , Y10T436/13 , Y10T436/143333
摘要： The present invention relates to a temperature-stable labeling reagent of formula: in which R1 represents H or an alkyl, aryl or substituted aryl group, R2 represents a detectable marker or at least two detectable markers linked together by at least one multimeric structure, L is a linking arm containing a linear succession of at least two covalent bonds and n an integer equal to 0 or 1, R3 and R4 represent independently of each other: H, NO2, Cl, Br, F, I, R2-(L)n-Y—X—, OR, SR, NR2, R, NHCOR, CONHR, COOR with R=alkyl or aryl, A is a linking arm containing at least one covalent double bond allowing conjugation of the diazo functional group with the aromatic ring and u is an integer between 0 and 2, preferably equal to 0 or 1, and —Y—X— represents —CONH—, —NHCO—, —CH2O—, —CH2S—. The present invention also describes a method for synthesizing said markers as well as applications for labeling biological molecules, in particular nucleic acids, with a labeling reagent carrying the diazomethyl functional group.The invention finds a preferred application in the field of diagnosis.
摘要翻译：本发明涉及下式的温度稳定的标记试剂：其中R 1表示H或烷基，芳基或取代的芳基，R 2表示可检测标记或至少两个通过至少一个多聚体结构连接在一起的可检测标记，L是含有至少两个共价键的线性连续并且n为等于0或1的整数的连接臂，R 3和 R 4彼此独立地表示：H，NO 2，Cl，Br，F，I，R 2 - （L）_{O-，-CH 2 S-。本发明还描述了一种用于合成所述标记的方法以及用携带重氮甲基官能团的标记试剂标记生物分子，特别是核酸的应用。本发明在诊断领域找到优选的应用。}

4. 发明申请

US20090215188A1 Method for labeling or treating a biological sample containing biological molecules of interest, in particular nucleic acids 有权
标题翻译：用于标记或处理含有感兴趣的生物分子，特别是核酸的生物样品的方法
公开(公告)号：US20090215188A1
公开(公告)日：2009-08-27
申请号：US11919668
申请日：2006-05-31
申请人： Eloy Bernal-Mendez , Ali Laayoun , Lionel Menou
发明人： Eloy Bernal-Mendez , Ali Laayoun , Lionel Menou
IPC分类号： G01N33/48 , C07H1/00
CPC分类号： G01N33/58 , G01N33/532 , G01N33/54353 , Y10T436/11 , Y10T436/143333
摘要： The present invention relates to a method for labeling biological molecules of interest contained in a biological sample, consisting in: a) providing a reaction vessel, b) immobilizing capture molecules, which are capable of binding a label of the biological molecules of interest, on a solid support of the vessel, c) introducing the biological sample but also at least one label of the biological molecules of interest into said vessel and optionally any ingredient required for labeling or prelabeling the molecules of interest, d) incubating the vessel content and immobilizing the label which is not reacted with the molecules of interest by binding to the capture molecules, and e) using the labeled molecules of interest for subsequent steps. A method for treating a biological sample is also disclosed.Said invention is preferably used in a manual or automated method for purifying nucleic acids.
摘要翻译：本发明涉及用于标记生物样品中所含感兴趣生物分子的方法，其包括：a）提供反应容器，b）使能够将感兴趣的生物分子的标记结合的捕获分子固定在所述容器的固体支持物，c）将所述生物学样品引入所述生物样品，还将所述生物分子的至少一个标记物引入所述容器，以及任选的任何标记或预标记目的分子所需的成分，d）孵育所述容器内容物并固定通过结合捕获分子不与感兴趣的分子反应的标记，和e）使用目的标记的分子进行后续步骤。还公开了一种用于处理生物样品的方法。所述发明优选以用于纯化核酸的手动或自动化方法使用。

5. 发明授权

US06902891B2 Process for labeling a nucleic acid 有权
标题翻译：核酸标记方法
公开(公告)号：US06902891B2
公开(公告)日：2005-06-07
申请号：US09736151
申请日：2000-12-15
申请人： Ali Laayoun , Lionel Menou , Christelle Tora , Aloke R. Banerjee , Michael M. Becker , Kenneth A. Browne , Matthew C. Friedenberg , Fred F. Hajjar
发明人： Ali Laayoun , Lionel Menou , Christelle Tora , Aloke R. Banerjee , Michael M. Becker , Kenneth A. Browne , Matthew C. Friedenberg , Fred F. Hajjar
IPC分类号： C07H21/00 , C12Q1/68 , C07D225/00 , C07D259/00 , C07H21/04 , C12P19/34
CPC分类号： C07H21/00 , C12Q2523/107
摘要： A process of fragmenting and labeling a synthetic or natural nucleic acid, comprising the steps of providing a mixture containing a nucleic acid, a labeling agent containing a detectable label, and at least one multivalent metal cation in a substantially aqueous solution; chemically fragmenting the nucleic acid in the mixture to produce a multiplicity of nucleic acid fragments; and attaching at least one label to at least one of the nucleic acid fragments to produce a detectably labeled nucleic acid fragment.
摘要翻译：包括以下步骤：提供含有核酸，含有可检测标记的标记试剂和至少一种多价金属阳离子在基本水溶液中的混合物的步骤; 将混合物中的核酸化学碎裂以产生多种核酸片段; 以及将至少一个标记附着到至少一个所述核酸片段以产生可检测地标记的核酸片段。

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