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    • 9. 发明授权
    • Mutant gene causing a defect in mitochondrial recombination and a method
for its detection
    • 导致线粒体重组缺陷的突变基因及其检测方法
    • US5908747A
    • 1999-06-01
    • US668448
    • 1996-06-21
    • Takehiko ShibataFeng LingFusao Makishima
    • Takehiko ShibataFeng LingFusao Makishima
    • A61K38/00C07K14/395C12N15/01C12N15/04C12Q1/68C07H21/04
    • C12N15/04C07K14/395C12N15/01A61K38/00
    • A multi-step method of detecting a gene in a nuclear chromosome which is involved in mitochondrial recombination is disclosed. The method disclosed includes the steps: a) fusing enucleated .omega..sup.- mitochondrial donor cells expressing a first marker gene with nucleus-containing .omega..sup.+ mitochondrial recipient cells expressing a second marker gene different from the first marker gene to form fused cells wherein the fused cells have one type of mitochondria and b) selecting fused cells that are .omega..sup.+ and express the first and second marker genes to identify those enucleated .omega..sup.- mitochondrial donor cells with a reduced recombination frequency. The method includes the further steps of judging that the gene in a nuclear chromosome of the recipient cells involved in mitochondrial recombination is normal when the mitochondrial recombination frequency is high or that the gene is mutated when the recombination frequency is low. The judging steps permit the detection of a mutant gene in a nuclear chromosome of the recipient cells used for cell fusion. An isolated mhr1 and MHR1 gene coding for a polypeptide is also disclosed.
    • 公开了一种检测参与线粒体重组的核染色体中的基因的多步法。 所公开的方法包括以下步骤:a)将表达第一标记基因的去核的ω-线粒体供体细胞与含有核的含有ω+线粒体受体细胞融合,其表达与第一标记基因不同的第二标记基因以形成融合细胞,其中融合细胞具有 一种类型的线粒体,以及b)选择ω+的融合细胞并表达第一和第二标记基因以鉴定具有降低的重组频率的那些去核的ω-线粒体供体细胞。 该方法包括以下步骤:当线粒体重组频率高时参与线粒体重组的受体细胞的核染色体中的基因正常,或者当重组频率低时基因突变。 判断步骤允许检测用于细胞融合的受体细胞的核染色体中的突变基因。 还公开了编码多肽的分离的mhr1和MHR1基因。